Restriction map for
pF1KB4699
(Circular) (Six-base) MAPSORT of: pF1KB4699.seq Check: 8546 from: 1 to: 4223
pF1KB4699 4223 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 710 1261 710
Size: 551 3672
Acc65I G'GTAC_C
Cuts at: 1239 1239
Size: 4223
AclI AA'CG_TT
Cuts at: 4063 4063
Size: 4223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 2048 2480 2833 3495 627
Size: 1421 432 353 662 1355
Fragments arranged by size:
1421 1355 662 432 353
AflIII A'CryG_T
Cuts at: 517 1575 3375 3656 517
Size: 1058 1800 281 1084
Fragments arranged by size:
1800 1084 1058 281
AlwNI CAG_nnn'CTG
Cuts at: 1078 1528 2966 1078
Size: 450 1438 2335
Fragments arranged by size:
2335 1438 450
ApaI G_GGCC'C
Cuts at: 1006 1006
Size: 4223
ApaLI G'TGCA_C
Cuts at: 3061 3061
Size: 4223
ApoI r'AATT_y
Cuts at: 1227 3576 3832 1227
Size: 2349 256 1618
Fragments arranged by size:
2349 1618 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4223
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
AvaI C'yCGr_G
Cuts at: 1243 1243
Size: 4223
BamHI G'GATC_C
Cuts at: 1248 1248
Size: 4223
BanI G'GyrC_C
Cuts at: 1239 1653 1901 1936 1239
Size: 414 248 35 3526
Fragments arranged by size:
3526 414 248 35
BanII G_rGCy'C
Cuts at: 1006 1237 2267 1006
Size: 231 1030 2962
Fragments arranged by size:
2962 1030 231
BbeI G_GCGC'C
Cuts at: 1657 1905 1657
Size: 248 3975
BbvCI CC'TCA_GC
Cuts at: 1144 1144
Size: 4223
BciVI GTATCCnnnnn_n'
Cuts at: 2121 3177 4223 2121
Size: 1056 1046 2121
Fragments arranged by size:
2121 1056 1046
BclI T'GATC_A
Cuts at: 1743 1743
Size: 4223
BglII A'GATC_T
Cuts at: 313 1738 3775 313
Size: 1425 2037 761
Fragments arranged by size:
2037 1425 761
BlpI GC'TnA_GC
Cuts at: 1335 1335
Size: 4223
Bme1580I G_kGCm'C
Cuts at: 592 1006 1848 1941 3065 592
Size: 414 842 93 1124 1750
Fragments arranged by size:
1750 1124 842 414 93
BmgBI CAC'GTC
Cuts at: 518 585 518
Size: 67 4156
BmrI ACTGGGnnnn_n'
Cuts at: 1435 1516 1851 3995 1435
Size: 81 335 2144 1663
Fragments arranged by size:
2144 1663 335 81
BmtI G_CTAG'C
Cuts at: 841 841
Size: 4223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 827
Size: 4223
Bpu10I CC'TnA_GC
Cuts at: 1144 1144
Size: 4223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1399 2278 2992 3290 700
Size: 699 879 714 298 1633
Fragments arranged by size:
1633 879 714 699 298
BsaI GGTCTCn'nnnn_
Cuts at: 3927 3927
Size: 4223
BsaAI yAC'GTr
Cuts at: 1576 1576
Size: 4223
BsaBI GATnn'nnATC
Cuts at: 6 1737 3774 6
Size: 1731 2037 455
Fragments arranged by size:
2037 1731 455
BsaHI Gr'CG_yC
Cuts at: 1654 1902 2604 4100 1654
Size: 248 702 1496 1777
Fragments arranged by size:
1777 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1412 1933 2611 3022 3169 3443 1412
Size: 521 678 411 147 274 2192
Fragments arranged by size:
2192 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 194 224 194
Size: 30 4193
BseYI C'CCAG_C
Cuts at: 876 1648 2406 3071 3479 876
Size: 772 758 665 408 1620
Fragments arranged by size:
1620 772 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 741 1765 1811 3041 104
Size: 637 1024 46 1230 1286
Fragments arranged by size:
1286 1230 1024 637 46
BsiHKAI G_wGCw'C
Cuts at: 270 1237 2015 2205 3065 270
Size: 967 778 190 860 1428
Fragments arranged by size:
1428 967 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 270 592 1006 1237 1848 1941 2015 2205 2267
Size: 322 414 231 611 93 74 190 62
Cuts at: 2267 3065 270
Size: 798 1428
Fragments arranged by size:
1428 798 611 414 322 231 190 93
74 62
BspHI T'CATG_A
Cuts at: 2655 2655
Size: 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1273 1487 1789 2170 1273
Size: 214 302 381 3326
Fragments arranged by size:
3326 381 302 214
BsrBI CCG'CTC
Cuts at: 50 638 2515 2569 4053 4218 50
Size: 588 1877 54 1484 165 55
Fragments arranged by size:
1877 1484 588 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2135 2135
Size: 4223
BsrFI r'CCGG_y
Cuts at: 2221 2402 2221
Size: 181 4042
BssSI C'ACGA_G
Cuts at: 2494 3202 2494
Size: 708 3515
BstAPI GCAn_nnn'nTGC
Cuts at: 1600 1600
Size: 4223
BstBI TT'CG_AA
Cuts at: 2584 2584
Size: 4223
BstEII G'GTnAC_C
Cuts at: 1190 1190
Size: 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 876 876
Size: 4223
BstYI r'GATC_y
Cuts at: 1 288 313 1248 1715 1738 2073 2319 2625
Size: 287 25 935 467 23 335 246 306
Cuts at: 2625 2637 2723 3775 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 935 467 449 335 306 287 246
86 25 23 12
Bsu36I CC'TnA_GG
Cuts at: 660 660
Size: 4223
BtgI C'CryG_G
Cuts at: 107 560 107
Size: 453 3770
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2173 2354 2517 2173
Size: 181 163 3879
Fragments arranged by size:
3879 181 163
BtsI GCAGTG_nn'
Cuts at: 428 1466 428
Size: 1038 3185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 687 722 854 889 687
Size: 35 132 35 4021
Fragments arranged by size:
4021 132 35 35
DraI TTT'AAA
Cuts at: 1222 1222
Size: 4223
DrdI GACnn_nn'nnGTC
Cuts at: 1929 3273 1929
Size: 1344 2879
EaeI y'GGCC_r
Cuts at: 557 816 1808 1982 2373 2400 3745 557
Size: 259 992 174 391 27 1345 1035
Fragments arranged by size:
1345 1035 992 391 259 174 27
EagI C'GGCC_G
Cuts at: 1808 1808
Size: 4223
EarI CTCTTCn'nnn_
Cuts at: 448 2246 2456 448
Size: 1798 210 2215
Fragments arranged by size:
2215 1798 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2431 3173 3319 4033 2431
Size: 742 146 714 2621
Fragments arranged by size:
2621 742 714 146
EcoICRI GAG'CTC
Cuts at: 1235 1235
Size: 4223
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 627 827 2048 2480 2833 3495 627
Size: 200 1221 432 353 662 1355
Fragments arranged by size:
1355 1221 662 432 353 200
EcoO109I rG'GnC_Cy
Cuts at: 856 1002 1003 1045 1362 856
Size: 146 1 42 317 3717
Fragments arranged by size:
3717 317 146 42 1
EcoRI G'AATT_C
Cuts at: 1227 1227
Size: 4223
EcoRV GAT'ATC
Cuts at: 306 306
Size: 4223
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1220 2242 1220
Size: 1022 3201
FspI TGC'GCA
Cuts at: 2004 2004
Size: 4223
HaeII r_GCGC'y
Cuts at: 1152 1657 1905 3135 3906 1152
Size: 505 248 1230 771 1469
Fragments arranged by size:
1469 1230 771 505 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2065 2095 2097 2127 2065
Size: 30 2 30 4161
Fragments arranged by size:
4161 30 30 2
HincII GTy'rAC
Cuts at: 1262 1262
Size: 4223
KasI G'GCGC_C
Cuts at: 1653 1901 1653
Size: 248 3975
KpnI G_GTAC'C
Cuts at: 1243 1243
Size: 4223
MfeI C'AATT_G
Cuts at: 831 831
Size: 4223
MluI A'CGCG_T
Cuts at: 3656 3656
Size: 4223
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 654 2982 3166 266
Size: 388 2328 184 1323
Fragments arranged by size:
2328 1323 388 184
MscI TGG'CCA
Cuts at: 559 818 1984 559
Size: 259 1166 2798
Fragments arranged by size:
2798 1166 259
MslI CAynn'nnrTG
Cuts at: 2212 2339 2212
Size: 127 4096
MspA1I CmG'CkG
Cuts at: 130 826 1075 1334 1648 2008 2792 3037 130
Size: 696 249 259 314 360 784 245 1316
Fragments arranged by size:
1316 784 696 360 314 259 249 245
NaeI GCC'GGC
Cuts at: 2404 2404
Size: 4223
NarI GG'CG_CC
Cuts at: 1654 1902 1654
Size: 248 3975
NcoI C'CATG_G
Cuts at: 107 560 107
Size: 453 3770
NgoMIV G'CCGG_C
Cuts at: 2402 2402
Size: 4223
NheI G'CTAG_C
Cuts at: 837 837
Size: 4223
NspI r_CATG'y
Cuts at: 1276 1776 3379 1276
Size: 500 1603 2120
Fragments arranged by size:
2120 1603 500
PciI A'CATG_T
Cuts at: 3375 3375
Size: 4223
PfoI T'CCnGG_A
Cuts at: 804 804
Size: 4223
PmeI GTTT'AAAC
Cuts at: 1222 1222
Size: 4223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 4191
PpuMI rG'GwC_Cy
Cuts at: 856 856
Size: 4223
PspOMI G'GGCC_C
Cuts at: 1002 1002
Size: 4223
PstI C_TGCA'G
Cuts at: 1180 1270 1955 1180
Size: 90 685 3448
Fragments arranged by size:
3448 685 90
PvuI CG_AT'CG
Cuts at: 104 741 104
Size: 637 3586
PvuII CAG'CTG
Cuts at: 130 826 1075 1648 2008 130
Size: 696 249 573 360 2345
Fragments arranged by size:
2345 696 573 360 249
RsrII CG'GwC_CG
Cuts at: 2418 2418
Size: 4223
SacI G_AGCT'C
Cuts at: 1237 1237
Size: 4223
SalI G'TCGA_C
Cuts at: 1260 1260
Size: 4223
SanDI GG'GwC_CC
Cuts at: 856 856
Size: 4223
SapI GCTCTTCn'nnn_
Cuts at: 448 2246 2456 448
Size: 1798 210 2215
Fragments arranged by size:
2215 1798 210
SbfI CC_TGCA'GG
Cuts at: 1270 1270
Size: 4223
ScaI AGT'ACT
Cuts at: 3740 3740
Size: 4223
SexAI A'CCwGG_T
Cuts at: 1010 1010
Size: 4223
SfcI C'TryA_G
Cuts at: 33 117 1049 1176 1266 1951 2919 3110 33
Size: 84 932 127 90 685 968 191 1146
Fragments arranged by size:
1146 968 932 685 191 127 90 84
SfoI GGC'GCC
Cuts at: 1655 1903 1655
Size: 248 3975
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4223
SmaI CCC'GGG
Cuts at: 1245 1245
Size: 4223
SmlI C'TyrA_G
Cuts at: 715 1378 2293 3007 3269 715
Size: 663 915 714 262 1669
Fragments arranged by size:
1669 915 714 663 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4223
SphI G_CATG'C
Cuts at: 1276 1776 1276
Size: 500 3723
StyI C'CwwG_G
Cuts at: 107 185 560 1195 1357 1711 107
Size: 78 375 635 162 354 2619
Fragments arranged by size:
2619 635 375 354 162 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 882 1806 1939 2398 2607 882
Size: 924 133 459 209 2498
Fragments arranged by size:
2498 924 459 209 133
TatI w'GTAC_w
Cuts at: 3738 3738
Size: 4223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 194 226 3740 3772 194
Size: 32 3514 32 645
Fragments arranged by size:
3514 645 32 32
Tth111I GACn'n_nGTC
Cuts at: 549 2020 549
Size: 1471 2752
XbaI T'CTAG_A
Cuts at: 1254 1254
Size: 4223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 1718
Size: 4223
XmaI C'CCGG_G
Cuts at: 1243 1243
Size: 4223
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstZ17I ClaI DraIII EcoNI FseI FspAI HindIII HpaI
NdeI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI