Restriction map for
pF1KB4700
(Circular) (Six-base) MAPSORT of: pF1KB4700.seq Check: 7780 from: 1 to: 5831
pF1KB4700 5831 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1141 1295 2869 1141
Size: 154 1574 4103
Fragments arranged by size:
4103 1574 154
Acc65I G'GTAC_C
Cuts at: 1645 2847 1645
Size: 1202 4629
AclI AA'CG_TT
Cuts at: 5671 5671
Size: 5831
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 239 496 3656 4088 4441 5103 148
Size: 91 257 3160 432 353 662 876
Fragments arranged by size:
3160 876 662 432 353 257 91
AfeI AGC'GCT
Cuts at: 128 128
Size: 5831
AflIII A'CryG_T
Cuts at: 1064 2804 3183 4983 5264 1064
Size: 1740 379 1800 281 1631
Fragments arranged by size:
1800 1740 1631 379 281
AgeI A'CCGG_T
Cuts at: 2272 2272
Size: 5831
AleI CACnn'nnGTG
Cuts at: 2239 2239
Size: 5831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1959 1959
Size: 5831
AlwNI CAG_nnn'CTG
Cuts at: 712 937 3136 4574 712
Size: 225 2199 1438 1969
Fragments arranged by size:
2199 1969 1438 225
ApaLI G'TGCA_C
Cuts at: 4669 4669
Size: 5831
ApoI r'AATT_y
Cuts at: 1526 1850 2113 2835 5184 5440 1526
Size: 324 263 722 2349 256 1917
Fragments arranged by size:
2349 1917 722 324 263 256
AseI AT'TA_AT
Cuts at: 20 833 1901 1970 2615 20
Size: 813 1068 69 645 3236
Fragments arranged by size:
3236 1068 813 645 69
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
AvaI C'yCGr_G
Cuts at: 753 2851 753
Size: 2098 3733
BamHI G'GATC_C
Cuts at: 2856 2856
Size: 5831
BanI G'GyrC_C
Cuts at: 1645 2269 2275 2847 3261 3509 3544 1645
Size: 624 6 572 414 248 35 3932
Fragments arranged by size:
3932 624 572 414 248 35 6
BanII G_rGCy'C
Cuts at: 2561 2722 2845 3875 2561
Size: 161 123 1030 4517
Fragments arranged by size:
4517 1030 161 123
BbeI G_GCGC'C
Cuts at: 3265 3513 3265
Size: 248 5583
BbsI GAAGACnn'nnnn_
Cuts at: 2456 2456
Size: 5831
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 224 234 258 268 982 1016 224
Size: 10 24 10 714 34 5039
Fragments arranged by size:
5039 714 34 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 2576 3729 4785 5831 2576
Size: 1153 1056 1046 2576
Fragments arranged by size:
2576 1153 1056 1046
BclI T'GATC_A
Cuts at: 3351 3351
Size: 5831
BglII A'GATC_T
Cuts at: 1883 3346 5383 1883
Size: 1463 2037 2331
Fragments arranged by size:
2331 2037 1463
BlpI GC'TnA_GC
Cuts at: 2093 2943 2093
Size: 850 4981
Bme1580I G_kGCm'C
Cuts at: 819 3456 3549 4673 819
Size: 2637 93 1124 1977
Fragments arranged by size:
2637 1977 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 597 710 2556 2784 3043 3124 3459 5603 597
Size: 113 1846 228 259 81 335 2144 825
Fragments arranged by size:
2144 1846 825 335 259 228 113 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4198 4198
Size: 5831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 1229 1937 2024 2615 606
Size: 623 708 87 591 3822
Fragments arranged by size:
3822 708 623 591 87
Bpu10I CC'TnA_GC
Cuts at: 1670 1670
Size: 5831
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 623 1901 1913 2487 2718 2736 3007 3886 4600
Size: 1278 12 574 231 18 271 879 714
Cuts at: 4600 4898 623
Size: 298 1556
Fragments arranged by size:
1556 1278 879 714 574 298 271 231
18 12
BsaI GGTCTCn'nnnn_
Cuts at: 430 5535 430
Size: 5105 726
BsaAI yAC'GTr
Cuts at: 3184 3184
Size: 5831
BsaBI GATnn'nnATC
Cuts at: 6 1331 3345 5382 6
Size: 1325 2014 2037 455
Fragments arranged by size:
2037 2014 1325 455
BsaHI Gr'CG_yC
Cuts at: 169 3262 3510 4212 5708 169
Size: 3093 248 702 1496 292
Fragments arranged by size:
3093 1496 702 292 248
BsaWI w'CCGG_w
Cuts at: 2272 2568 3020 3541 4219 4630 4777 5051 2272
Size: 296 452 521 678 411 147 274 3052
Fragments arranged by size:
3052 678 521 452 411 296 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 2456 508
Size: 1948 3883
BseYI C'CCAG_C
Cuts at: 2707 3256 4014 4679 5087 2707
Size: 549 758 665 408 3451
Fragments arranged by size:
3451 758 665 549 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 254 2217 254
Size: 1963 3868
BsiEI CG_ry'CG
Cuts at: 104 1211 3373 3419 4649 104
Size: 1107 2162 46 1230 1286
Fragments arranged by size:
2162 1286 1230 1107 46
BsiHKAI G_wGCw'C
Cuts at: 2722 2845 3623 3813 4673 2722
Size: 123 778 190 860 3880
Fragments arranged by size:
3880 860 778 190 123
Bsp1286I G_dGCh'C
Cuts at: 819 2561 2722 2845 3456 3549 3623 3813 3875
Size: 1742 161 123 611 93 74 190 62
Cuts at: 3875 4673 819
Size: 798 1977
Fragments arranged by size:
1977 1742 798 611 190 161 123 93
74 62
BspHI T'CATG_A
Cuts at: 4263 4263
Size: 5831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2881 3095 3397 3778 2881
Size: 214 302 381 4934
Fragments arranged by size:
4934 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4123 4177 5661 5826 50
Size: 4073 54 1484 165 55
Fragments arranged by size:
4073 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 303 2282 3743 303
Size: 1979 1461 2391
Fragments arranged by size:
2391 1979 1461
BsrFI r'CCGG_y
Cuts at: 1642 2272 3829 4010 1642
Size: 630 1557 181 3463
Fragments arranged by size:
3463 1557 630 181
BsrGI T'GTAC_A
Cuts at: 1364 1364
Size: 5831
BssSI C'ACGA_G
Cuts at: 4102 4810 4102
Size: 708 5123
BstAPI GCAn_nnn'nTGC
Cuts at: 985 3208 985
Size: 2223 3608
BstBI TT'CG_AA
Cuts at: 4192 4192
Size: 5831
BstYI r'GATC_y
Cuts at: 1 1383 1746 1883 2856 3323 3346 3681 3927
Size: 1382 363 137 973 467 23 335 246
Cuts at: 3927 4233 4245 4331 5383 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1382 1052 973 467 449 363 335 306
246 137 86 23 12
BstZ17I GTA'TAC
Cuts at: 1142 1142
Size: 5831
Bsu36I CC'TnA_GG
Cuts at: 938 938
Size: 5831
BtgI C'CryG_G
Cuts at: 1205 1442 2782 1205
Size: 237 1340 4254
Fragments arranged by size:
4254 1340 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3781 3962 4125 3781
Size: 181 163 5487
Fragments arranged by size:
5487 181 163
BtsI GCAGTG_nn'
Cuts at: 333 682 1367 1664 2700 2702 3074 333
Size: 349 685 297 1036 2 372 3090
Fragments arranged by size:
3090 1036 685 372 349 297 2
ClaI AT'CG_AT
Cuts at: 1683 1683
Size: 5831
DraI TTT'AAA
Cuts at: 2105 2420 2830 2105
Size: 315 410 5106
Fragments arranged by size:
5106 410 315
DrdI GACnn_nn'nnGTC
Cuts at: 1435 3537 4881 1435
Size: 2102 1344 2385
Fragments arranged by size:
2385 2102 1344
EaeI y'GGCC_r
Cuts at: 561 1208 3416 3590 3981 4008 5353 561
Size: 647 2208 174 391 27 1345 1039
Fragments arranged by size:
2208 1345 1039 647 391 174 27
EagI C'GGCC_G
Cuts at: 1208 3416 1208
Size: 2208 3623
EarI CTCTTCn'nnn_
Cuts at: 275 399 574 3854 4064 275
Size: 124 175 3280 210 2042
Fragments arranged by size:
3280 2042 210 175 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1394 4039 4781 4927 5641 1394
Size: 2645 742 146 714 1584
Fragments arranged by size:
2645 1584 742 714 146
EcoICRI GAG'CTC
Cuts at: 2720 2843 2720
Size: 123 5708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 239 496 606 1229 1937 2024 2615 3656
Size: 91 257 110 623 708 87 591 1041
Cuts at: 3656 4088 4441 5103 148
Size: 432 353 662 876
Fragments arranged by size:
1041 876 708 662 623 591 432 353
257 110 91 87
EcoNI CCTnn'n_nnAGG
Cuts at: 163 163
Size: 5831
EcoO109I rG'GnC_Cy
Cuts at: 628 1674 2622 2711 2970 628
Size: 1046 948 89 259 3489
Fragments arranged by size:
3489 1046 948 259 89
EcoRI G'AATT_C
Cuts at: 1526 1850 2835 1526
Size: 324 985 4522
Fragments arranged by size:
4522 985 324
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 501 1070 1651 3850 501
Size: 569 581 2199 2482
Fragments arranged by size:
2482 2199 581 569
FspI TGC'GCA
Cuts at: 3612 3612
Size: 5831
HaeII r_GCGC'y
Cuts at: 130 3265 3513 4743 5514 130
Size: 3135 248 1230 771 447
Fragments arranged by size:
3135 1230 771 447 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 575 607 1777 1809 2055 2087 3673 3703 3705
Size: 32 1170 32 246 32 1586 30 2
Cuts at: 3705 3735 575
Size: 30 2671
Fragments arranged by size:
2671 1586 1170 246 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1547 1655 2870 1547
Size: 108 1215 4508
Fragments arranged by size:
4508 1215 108
HindIII A'AGCT_T
Cuts at: 381 381
Size: 5831
KasI G'GCGC_C
Cuts at: 3261 3509 3261
Size: 248 5583
KpnI G_GTAC'C
Cuts at: 1649 2851 1649
Size: 1202 4629
MfeI C'AATT_G
Cuts at: 980 1731 1772 980
Size: 751 41 5039
Fragments arranged by size:
5039 751 41
MluI A'CGCG_T
Cuts at: 5264 5264
Size: 5831
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 748 2246 2550 4590 4774 748
Size: 1498 304 2040 184 1805
Fragments arranged by size:
2040 1805 1498 304 184
MscI TGG'CCA
Cuts at: 563 3592 563
Size: 3029 2802
MslI CAynn'nnrTG
Cuts at: 1102 1219 2239 3820 3947 1102
Size: 117 1020 1581 127 2986
Fragments arranged by size:
2986 1581 1020 127 117
MspA1I CmG'CkG
Cuts at: 147 762 1111 2475 2942 3256 3616 4400 4645
Size: 615 349 1364 467 314 360 784 245
Cuts at: 4645 147
Size: 1333
Fragments arranged by size:
1364 1333 784 615 467 360 349 314
245
NaeI GCC'GGC
Cuts at: 4012 4012
Size: 5831
NarI GG'CG_CC
Cuts at: 3262 3510 3262
Size: 248 5583
NdeI CA'TA_TG
Cuts at: 960 1185 2661 960
Size: 225 1476 4130
Fragments arranged by size:
4130 1476 225
NgoMIV G'CCGG_C
Cuts at: 4010 4010
Size: 5831
NruI TCG'CGA
Cuts at: 788 788
Size: 5831
NspI r_CATG'y
Cuts at: 406 823 1068 2808 2884 3384 4987 406
Size: 417 245 1740 76 500 1603 1250
Fragments arranged by size:
1740 1603 1250 500 417 245 76
PciI A'CATG_T
Cuts at: 1064 2804 4983 1064
Size: 1740 2179 1912
Fragments arranged by size:
2179 1912 1740
PflMI CCAn_nnn'nTGG
Cuts at: 2008 2593 2008
Size: 585 5246
PmeI GTTT'AAAC
Cuts at: 2830 2830
Size: 5831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 4277
Size: 32 5799
PpuMI rG'GwC_Cy
Cuts at: 628 1674 2711 628
Size: 1046 1037 3748
Fragments arranged by size:
3748 1046 1037
PshAI GACnn'nnGTC
Cuts at: 1228 1228
Size: 5831
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1348 1380 1348
Size: 32 5799
PstI C_TGCA'G
Cuts at: 339 1500 1883 2480 2583 2878 3563 339
Size: 1161 383 597 103 295 685 2607
Fragments arranged by size:
2607 1161 685 597 383 295 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5831
PvuII CAG'CTG
Cuts at: 762 1111 2475 3256 3616 762
Size: 349 1364 781 360 2977
Fragments arranged by size:
2977 1364 781 360 349
RsrII CG'GwC_CG
Cuts at: 2571 4026 2571
Size: 1455 4376
SacI G_AGCT'C
Cuts at: 2722 2845 2722
Size: 123 5708
SalI G'TCGA_C
Cuts at: 2868 2868
Size: 5831
SapI GCTCTTCn'nnn_
Cuts at: 3854 4064 3854
Size: 210 5621
SbfI CC_TGCA'GG
Cuts at: 2878 2878
Size: 5831
ScaI AGT'ACT
Cuts at: 5348 5348
Size: 5831
SexAI A'CCwGG_T
Cuts at: 914 914
Size: 5831
SfcI C'TryA_G
Cuts at: 33 173 335 1160 1496 1879 2476 2579 2874
Size: 140 162 825 336 383 597 103 295
Cuts at: 2874 3559 4527 4718 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 825 685 597 383 336 295
191 162 140 103
SfoI GGC'GCC
Cuts at: 3263 3511 3263
Size: 248 5583
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
SgrAI Cr'CCGG_yG
Cuts at: 2272 2272
Size: 5831
SmaI CCC'GGG
Cuts at: 2853 2853
Size: 5831
SmlI C'TyrA_G
Cuts at: 638 1916 1928 2466 2715 2733 2986 3901 4615
Size: 1278 12 538 249 18 253 915 714
Cuts at: 4615 4877 638
Size: 262 1592
Fragments arranged by size:
1592 1278 915 714 538 262 253 249
18 12
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5831
SphI G_CATG'C
Cuts at: 406 2884 3384 406
Size: 2478 500 2853
Fragments arranged by size:
2853 2478 500
SspI AAT'ATT
Cuts at: 1701 1974 2184 2415 1701
Size: 273 210 231 5117
Fragments arranged by size:
5117 273 231 210
StyI C'CwwG_G
Cuts at: 1520 2965 3319 1520
Size: 1445 354 4032
Fragments arranged by size:
4032 1445 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1074 1339 1426 1802 2245 3414 3547 4006 4215
Size: 265 87 376 443 1169 133 459 209
Cuts at: 4215 1074
Size: 2690
Fragments arranged by size:
2690 1169 459 443 376 265 209 133
87
TatI w'GTAC_w
Cuts at: 312 1364 5346 312
Size: 1052 3982 797
Fragments arranged by size:
3982 1052 797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 830 862 947 979 2241 2273 5348 5380 830
Size: 32 85 32 1262 32 3075 32 1281
Fragments arranged by size:
3075 1281 1262 85 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 589 865 3628 589
Size: 276 2763 2792
Fragments arranged by size:
2792 2763 276
XbaI T'CTAG_A
Cuts at: 2862 2862
Size: 5831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 724 922 2514 3326 724
Size: 198 1592 812 3229
Fragments arranged by size:
3229 1592 812 198
XmaI C'CCGG_G
Cuts at: 2851 2851
Size: 5831
XmnI GAAnn'nnTTC
Cuts at: 1634 1634
Size: 5831
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstXI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NheI NotI NsiI
PacI PasI PfoI PmlI PsiI PspOMI PspXI SacII
SanDI SfiI SnaBI SrfI StuI SwaI XhoI ZraI