Restriction map for
pF1KB4702
(Circular) (Six-base) MAPSORT of: pF1KB4702.seq Check: 7025 from: 1 to: 4835
pF1KB4702 4835 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 146 1873 146
Size: 1727 3108
Acc65I G'GTAC_C
Cuts at: 1851 1851
Size: 4835
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2660 3092 3445 4107 2660
Size: 432 353 662 3388
Fragments arranged by size:
3388 662 432 353
AflIII A'CryG_T
Cuts at: 933 2187 3987 4268 933
Size: 1254 1800 281 1500
Fragments arranged by size:
1800 1500 1254 281
AleI CACnn'nnGTG
Cuts at: 708 708
Size: 4835
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 336 336
Size: 4835
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 224
Size: 32 4803
AlwNI CAG_nnn'CTG
Cuts at: 946 2140 3578 946
Size: 1194 1438 2203
Fragments arranged by size:
2203 1438 1194
ApaLI G'TGCA_C
Cuts at: 3673 3673
Size: 4835
ApoI r'AATT_y
Cuts at: 381 1319 1390 1453 1839 4188 4444 381
Size: 938 71 63 386 2349 256 772
Fragments arranged by size:
2349 938 772 386 256 71 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 1855 1855
Size: 4835
AvrII C'CTAG_G
Cuts at: 348 348
Size: 4835
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 1851 2265 2513 2548 1851
Size: 414 248 35 4138
Fragments arranged by size:
4138 414 248 35
BanII G_rGCy'C
Cuts at: 1849 2879 1849
Size: 1030 3805
BbeI G_GCGC'C
Cuts at: 2269 2517 2269
Size: 248 4587
BciVI GTATCCnnnnn_n'
Cuts at: 2733 3789 4835 2733
Size: 1056 1046 2733
Fragments arranged by size:
2733 1056 1046
BclI T'GATC_A
Cuts at: 2355 2355
Size: 4835
BglI GCCn_nnn'nGGC
Cuts at: 161 161
Size: 4835
BglII A'GATC_T
Cuts at: 2350 4387 2350
Size: 2037 2798
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 2460 2553 3677 2460
Size: 93 1124 3618
Fragments arranged by size:
3618 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2047 2128 2463 4607 2047
Size: 81 335 2144 2275
Fragments arranged by size:
2275 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 855 3202 855
Size: 2347 2488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1682 1832 488
Size: 1194 150 3491
Fragments arranged by size:
3491 1194 150
Bpu10I CC'TnA_GC
Cuts at: 1069 1069
Size: 4835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 1508 2011 2890 3604 3902 545
Size: 963 503 879 714 298 1478
Fragments arranged by size:
1478 963 879 714 503 298
BsaI GGTCTCn'nnnn_
Cuts at: 4539 4539
Size: 4835
BsaAI yAC'GTr
Cuts at: 2188 2188
Size: 4835
BsaBI GATnn'nnATC
Cuts at: 6 2349 4386 6
Size: 2343 2037 455
Fragments arranged by size:
2343 2037 455
BsaHI Gr'CG_yC
Cuts at: 2266 2514 3216 4712 2266
Size: 248 702 1496 2389
Fragments arranged by size:
2389 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2024 2545 3223 3634 3781 4055 2024
Size: 521 678 411 147 274 2804
Fragments arranged by size:
2804 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 128 158 224 254 1685 1715 128
Size: 30 66 30 1431 30 3248
Fragments arranged by size:
3248 1431 66 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 902 902
Size: 4835
BseYI C'CCAG_C
Cuts at: 2260 3018 3683 4091 2260
Size: 758 665 408 3004
Fragments arranged by size:
3004 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 892
Size: 4835
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3653 104
Size: 2273 46 1230 1286
Fragments arranged by size:
2273 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 550 643 1668 1849 2627 2817 3677 550
Size: 93 1025 181 778 190 860 1708
Fragments arranged by size:
1708 1025 860 778 190 181 93
BsmI GAATG_Cn'
Cuts at: 753 960 753
Size: 207 4628
Bsp1286I G_dGCh'C
Cuts at: 550 643 1668 1849 2460 2553 2627 2817 2879
Size: 93 1025 181 611 93 74 190 62
Cuts at: 2879 3677 550
Size: 798 1708
Fragments arranged by size:
1708 1025 798 611 190 181 93 93
74 62
BspHI T'CATG_A
Cuts at: 276 624 3267 276
Size: 348 2643 1844
Fragments arranged by size:
2643 1844 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1885 2099 2401 2782 1885
Size: 214 302 381 3938
Fragments arranged by size:
3938 381 302 214
BsrBI CCG'CTC
Cuts at: 50 139 3127 3181 4665 4830 50
Size: 89 2988 54 1484 165 55
Fragments arranged by size:
2988 1484 165 89 55 54
BsrDI GCAATG_nn'
Cuts at: 1204 2747 1204
Size: 1543 3292
BsrFI r'CCGG_y
Cuts at: 155 2833 3014 155
Size: 2678 181 1976
Fragments arranged by size:
2678 1976 181
BssSI C'ACGA_G
Cuts at: 3106 3814 3106
Size: 708 4127
BstAPI GCAn_nnn'nTGC
Cuts at: 654 955 2212 654
Size: 301 1257 3277
Fragments arranged by size:
3277 1257 301
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstYI r'GATC_y
Cuts at: 1 782 1860 2327 2350 2685 2931 3237 3249
Size: 781 1078 467 23 335 246 306 12
Cuts at: 3249 3335 4387 1
Size: 86 1052 449
Fragments arranged by size:
1078 1052 781 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4835
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 810 2078 810
Size: 1268 3567
DraI TTT'AAA
Cuts at: 1834 1834
Size: 4835
DraIII CAC_nnn'GTG
Cuts at: 530 654 530
Size: 124 4711
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3885 2541
Size: 1344 3491
EaeI y'GGCC_r
Cuts at: 153 261 1719 2420 2594 2985 3012 4357 153
Size: 108 1458 701 174 391 27 1345 631
Fragments arranged by size:
1458 1345 701 631 391 174 108 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 303 305 2858 3068 303
Size: 2 2553 210 2070
Fragments arranged by size:
2553 2070 210 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 3785 3931 4645 3043
Size: 742 146 714 3233
Fragments arranged by size:
3233 742 714 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1682 1832 2660 3092 3445 4107 488
Size: 1194 150 828 432 353 662 1216
Fragments arranged by size:
1216 1194 828 662 432 353 150
EcoO109I rG'GnC_Cy
Cuts at: 1974 1974
Size: 4835
EcoRI G'AATT_C
Cuts at: 1390 1839 1390
Size: 449 4386
EcoRV GAT'ATC
Cuts at: 464 464
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 523 2854 523
Size: 2331 2504
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 2269 2517 3747 4518 2269
Size: 248 1230 771 2586
Fragments arranged by size:
2586 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 881 913 1774 1806 2677 2707 2709
Size: 32 388 32 861 32 871 30 2
Cuts at: 2709 2739 461
Size: 30 2557
Fragments arranged by size:
2557 871 861 388 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1601 1874 1601
Size: 273 4562
HpaI GTT'AAC
Cuts at: 1601 1601
Size: 4835
KasI G'GCGC_C
Cuts at: 2265 2513 2265
Size: 248 4587
KpnI G_GTAC'C
Cuts at: 1855 1855
Size: 4835
MfeI C'AATT_G
Cuts at: 1412 1412
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 843 1235 1432 1760 3594 3778 843
Size: 392 197 328 1834 184 1900
Fragments arranged by size:
1900 1834 392 328 197 184
MscI TGG'CCA
Cuts at: 263 2596 263
Size: 2333 2502
MslI CAynn'nnrTG
Cuts at: 708 1049 2824 2951 708
Size: 341 1775 127 2592
Fragments arranged by size:
2592 1775 341 127
MspA1I CmG'CkG
Cuts at: 178 1946 2260 2620 3404 3649 178
Size: 1768 314 360 784 245 1364
Fragments arranged by size:
1768 1364 784 360 314 245
NaeI GCC'GGC
Cuts at: 157 3016 157
Size: 2859 1976
NarI GG'CG_CC
Cuts at: 2266 2514 2266
Size: 248 4587
NcoI C'CATG_G
Cuts at: 107 107
Size: 4835
NgoMIV G'CCGG_C
Cuts at: 155 3014 155
Size: 2859 1976
NspI r_CATG'y
Cuts at: 661 937 1888 2388 3991 661
Size: 276 951 500 1603 1505
Fragments arranged by size:
1603 1505 951 500 276
PciI A'CATG_T
Cuts at: 933 3987 933
Size: 3054 1781
PflMI CCAn_nnn'nTGG
Cuts at: 1414 1414
Size: 4835
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 224 256 3281 3313 224
Size: 32 3025 32 1746
Fragments arranged by size:
3025 1746 32 32
PstI C_TGCA'G
Cuts at: 416 447 951 1119 1882 2567 416
Size: 31 504 168 763 685 2684
Fragments arranged by size:
2684 763 685 504 168 31
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 178 2260 2620 178
Size: 2082 360 2393
Fragments arranged by size:
2393 2082 360
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 303 2858 3068 303
Size: 2555 210 2070
Fragments arranged by size:
2555 2070 210
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 4352 4352
Size: 4835
SexAI A'CCwGG_T
Cuts at: 650 650
Size: 4835
SfcI C'TryA_G
Cuts at: 33 179 412 443 947 1115 1433 1878 2563
Size: 146 233 31 504 168 318 445 685
Cuts at: 2563 3531 3722 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 504 445 318 233 191
168 146 31
SfoI GGC'GCC
Cuts at: 2267 2515 2267
Size: 248 4587
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 1857 1857
Size: 4835
SmlI C'TyrA_G
Cuts at: 560 1523 1990 2905 3619 3881 560
Size: 963 467 915 714 262 1514
Fragments arranged by size:
1514 963 915 714 467 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 661 1888 2388 661
Size: 1227 500 3108
Fragments arranged by size:
3108 1227 500
StyI C'CwwG_G
Cuts at: 107 348 1969 2323 107
Size: 241 1621 354 2619
Fragments arranged by size:
2619 1621 354 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2418 2551 3010 3219 2418
Size: 133 459 209 4034
Fragments arranged by size:
4034 459 209 133
TatI w'GTAC_w
Cuts at: 4350 4350
Size: 4835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4352 4384 4352
Size: 32 4803
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2330 114
Size: 2216 2619
XmaI C'CCGG_G
Cuts at: 1855 1855
Size: 4835
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI