FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KB4708, 444 aa 1>>>pF1KB4708 444 - 444 aa - 444 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 5.0400+/-0.000433; mu= 18.4644+/- 0.027 mean_var=57.4569+/-12.231, 0's: 0 Z-trim(109.8): 37 B-trim: 509 in 1/49 Lambda= 0.169201 statistics sampled from 18058 (18086) to 18058 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.577), E-opt: 0.2 (0.212), width: 16 Scan time: 8.100 The best scores are: opt bits E(85289) NP_004256 (OMIM: 606149) fatty acid desaturase 2 i ( 444) 3118 769.8 0 NP_001268430 (OMIM: 606149) fatty acid desaturase ( 422) 2641 653.3 3.4e-187 NP_001268431 (OMIM: 606149) fatty acid desaturase ( 413) 2637 652.3 6.6e-187 NP_068373 (OMIM: 606150) fatty acid desaturase 3 [ ( 445) 2051 509.3 8.1e-144 XP_011543325 (OMIM: 606150) PREDICTED: fatty acid ( 454) 2014 500.3 4.3e-141 NP_037534 (OMIM: 606148) fatty acid desaturase 1 [ ( 501) 1984 493.0 7.5e-139 XP_016873213 (OMIM: 606150) PREDICTED: fatty acid ( 491) 1793 446.4 8e-125 XP_016873212 (OMIM: 606150) PREDICTED: fatty acid ( 500) 1756 437.3 4.3e-122 XP_011543324 (OMIM: 606148) PREDICTED: fatty acid ( 430) 1753 436.6 6.2e-122 NP_683725 (OMIM: 250790,613218) cytochrome b5 isof ( 134) 210 59.7 5.6e-09 NP_001177736 (OMIM: 250790,613218) cytochrome b5 i ( 124) 208 59.2 7.3e-09 XP_011524137 (OMIM: 250790,613218) PREDICTED: cyto ( 98) 206 58.6 8.4e-09 NP_001905 (OMIM: 250790,613218) cytochrome b5 isof ( 98) 206 58.6 8.4e-09 NP_085056 (OMIM: 611964) cytochrome b5 type B [Hom ( 150) 203 58.0 2e-08 NP_057314 (OMIM: 608343) cytochrome b5 reductase 4 ( 521) 165 49.0 3.6e-05 >>NP_004256 (OMIM: 606149) fatty acid desaturase 2 isofo (444 aa) initn: 3118 init1: 3118 opt: 3118 Z-score: 4110.6 bits: 769.8 E(85289): 0 Smith-Waterman score: 3118; 100.0% identity (100.0% similar) in 444 aa overlap (1-444:1-444) 10 20 30 40 50 60 pF1KB4 MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVI 10 20 30 40 50 60 70 80 90 100 110 120 pF1KB4 GHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 GHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTA 70 80 90 100 110 120 130 140 150 160 170 180 pF1KB4 EDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 EDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQH 130 140 150 160 170 180 190 200 210 220 230 240 pF1KB4 DYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 DYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFV 190 200 210 220 230 240 250 260 270 280 290 300 pF1KB4 LGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 LGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAV 250 260 270 280 290 300 310 320 330 340 350 360 pF1KB4 SYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 SYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQL 310 320 330 340 350 360 370 380 390 400 410 420 pF1KB4 TATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_004 TATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLL 370 380 390 400 410 420 430 440 pF1KB4 RALLDIIRSLKKSGKLWLDAYLHK :::::::::::::::::::::::: NP_004 RALLDIIRSLKKSGKLWLDAYLHK 430 440 >>NP_001268430 (OMIM: 606149) fatty acid desaturase 2 is (422 aa) initn: 2637 init1: 2637 opt: 2641 Z-score: 3481.7 bits: 653.3 E(85289): 3.4e-187 Smith-Waterman score: 2641; 96.7% identity (98.0% similar) in 393 aa overlap (53-444:35-422) 30 40 50 60 70 80 pF1KB4 WEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGH-YAGEDATDAFRAFHPDLEF ::.. :: .:.. :::::::::::: NP_001 EAGPFVCVCVLLASIPTPQTPLLQASLPPFHPASA---GHPITGQQ--DAFRAFHPDLEF 10 20 30 40 50 90 100 110 120 130 140 pF1KB4 VGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHII :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 VGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHII 60 70 80 90 100 110 150 160 170 180 190 200 pF1KB4 ALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 ALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFV 120 130 140 150 160 170 210 220 230 240 250 260 pF1KB4 IGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 IGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNH 180 190 200 210 220 230 270 280 290 300 310 320 pF1KB4 QHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 QHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALL 240 250 260 270 280 290 330 340 350 360 370 380 pF1KB4 FLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 FLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNF 300 310 320 330 340 350 390 400 410 420 430 440 pF1KB4 QIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAY :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 QIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAY 360 370 380 390 400 410 pF1KB4 LHK ::: NP_001 LHK 420 >>NP_001268431 (OMIM: 606149) fatty acid desaturase 2 is (413 aa) initn: 2637 init1: 2637 opt: 2637 Z-score: 3476.6 bits: 652.3 E(85289): 6.6e-187 Smith-Waterman score: 2637; 100.0% identity (100.0% similar) in 375 aa overlap (70-444:39-413) 40 50 60 70 80 90 pF1KB4 DRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEP :::::::::::::::::::::::::::::: NP_001 RRVNSLMLYTLRSITSHRSSHPERWATSSQDAFRAFHPDLEFVGKFLKPLLIGELAPEEP 10 20 30 40 50 60 100 110 120 130 140 150 pF1KB4 SQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 SQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWI 70 80 90 100 110 120 160 170 180 190 200 210 pF1KB4 PTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 PTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQ 130 140 150 160 170 180 220 230 240 250 260 270 pF1KB4 HHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 HHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYF 190 200 210 220 230 240 280 290 300 310 320 330 pF1KB4 QYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 QYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQ 250 260 270 280 290 300 340 350 360 370 380 390 pF1KB4 MNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKI 310 320 330 340 350 360 400 410 420 430 440 pF1KB4 APLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK ::::::::::::::::::::::::::::::::::::::::::::: NP_001 APLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK 370 380 390 400 410 >>NP_068373 (OMIM: 606150) fatty acid desaturase 3 [Homo (445 aa) initn: 2076 init1: 1064 opt: 2051 Z-score: 2703.0 bits: 509.3 E(85289): 8.1e-144 Smith-Waterman score: 2051; 62.6% identity (84.1% similar) in 446 aa overlap (1-444:1-445) 10 20 30 40 50 pF1KB4 MGKGGNQG--EGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQR :: :. : :: :. . .::: ::.:. :. :.::::.:.::.:..:. .::::.: NP_068 MGGVGEPGPREGPAQPGAPLPTFCWEQIRAHDQPGDKWLVIERRVYDISRWAQRHPGGSR 10 20 30 40 50 60 60 70 80 90 100 110 pF1KB4 VIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRK .:::...::::::::::: ::.:: :::.::::::::::::::: :....::::::.. NP_068 LIGHHGAEDATDAFRAFHQDLNFVRKFLQPLLIGELAPEEPSQDGPLNAQLVEDFRALHQ 70 80 90 100 110 120 120 130 140 150 160 170 pF1KB4 TAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWL .::::.:: .. .:: .::.::.:.: .::. .. .: ::.:. ..::.:: ::::. : NP_068 AAEDMKLFDASPTFFAFLLGHILAMEVLAWLLIYLLGPGWVPSALAAFILAISQAQSWCL 130 140 150 160 170 180 180 190 200 210 220 230 pF1KB4 QHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHV ::: :: :...: :::...:::.:.::: ::.::: ::::::::::::::::::.. : NP_068 QHDLGHASIFKKSWWNHVAQKFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPV 190 200 210 220 230 240 240 250 260 270 280 290 pF1KB4 FVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAW :.::: . .:::::: .:::::.:: :::::::::: . :. . . :.: .:.:: : NP_068 FLLGE-SSVEYGKKKRRYLPYNQQHLYFFLIGPPLLTLVNFEVENLAYMLVCMQWADLLW 250 260 270 280 290 300 310 320 330 340 350 pF1KB4 AVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSS :.:.: :::..:.::::. :.:::. .: ::::::::.:::::: :: .: .::: :: NP_068 AASFYARFFLSYLPFYGVPGVLLFFVAVRVLESHWFVWITQMNHIPKEIGHEKHRDWVSS 300 310 320 330 340 350 360 370 380 390 400 410 pF1KB4 QLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKP ::.:::::: :.:..:::::::::::::::: ::::: ..::::::::::::. :. :: NP_068 QLAATCNVEPSLFTNWFSGHLNFQIEHHLFPRMPRHNYSRVAPLVKSLCAKHGLSYEVKP 360 370 380 390 400 410 420 430 440 pF1KB4 LLRALLDIIRSLKKSGKLWLDAYLHK .: ::.::.::::::: .:::::::. NP_068 FLTALVDIVRSLKKSGDIWLDAYLHQ 420 430 440 >>XP_011543325 (OMIM: 606150) PREDICTED: fatty acid desa (454 aa) initn: 2053 init1: 1064 opt: 2014 Z-score: 2654.0 bits: 500.3 E(85289): 4.3e-141 Smith-Waterman score: 2014; 61.1% identity (82.4% similar) in 455 aa overlap (1-444:1-454) 10 20 30 40 50 pF1KB4 MGKGGNQG--EGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQR :: :. : :: :. . .::: ::.:. :. :.::::.:.::.:..:. .::::.: XP_011 MGGVGEPGPREGPAQPGAPLPTFCWEQIRAHDQPGDKWLVIERRVYDISRWAQRHPGGSR 10 20 30 40 50 60 60 70 80 90 100 110 pF1KB4 VIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRK .:::...::::::::::: ::.:: :::.::::::::::::::: :....::::::.. XP_011 LIGHHGAEDATDAFRAFHQDLNFVRKFLQPLLIGELAPEEPSQDGPLNAQLVEDFRALHQ 70 80 90 100 110 120 120 130 140 150 160 170 pF1KB4 TAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWL .::::.:: .. .:: .::.::.:.: .::. .. .: ::.:. ..::.:: ::::. : XP_011 AAEDMKLFDASPTFFAFLLGHILAMEVLAWLLIYLLGPGWVPSALAAFILAISQAQSWCL 130 140 150 160 170 180 180 190 200 210 220 230 pF1KB4 QHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHV ::: :: :...: :::...:::.:.::: ::.::: ::::::::::::::::::.. : XP_011 QHDLGHASIFKKSWWNHVAQKFVMGQLKGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPV 190 200 210 220 230 240 240 250 260 270 280 290 pF1KB4 FVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAW :.::: . .:::::: .:::::.:: :::::::::: . :. . . :.: .:.:: : XP_011 FLLGE-SSVEYGKKKRRYLPYNQQHLYFFLIGPPLLTLVNFEVENLAYMLVCMQWADLLW 250 260 270 280 290 300 310 320 330 340 350 pF1KB4 AVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSS :.:.: :::..:.::::. :.:::. .: ::::::::.:::::: :: .: .::: :: XP_011 AASFYARFFLSYLPFYGVPGVLLFFVAVRVLESHWFVWITQMNHIPKEIGHEKHRDWVSS 300 310 320 330 340 350 360 370 380 390 400 pF1KB4 QLTATCNVEQSFFNDWFSGHLNFQIEHH---------LFPTMPRHNLHKIAPLVKSLCAK ::.:::::: :.:..::::::::::::. ::: ::::: ..:::::::::: XP_011 QLAATCNVEPSLFTNWFSGHLNFQIEHQCQHTLPTPSLFPRMPRHNYSRVAPLVKSLCAK 360 370 380 390 400 410 410 420 430 440 pF1KB4 HGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK ::. :. ::.: ::.::.::::::: .:::::::. XP_011 HGLSYEVKPFLTALVDIVRSLKKSGDIWLDAYLHQ 420 430 440 450 >>NP_037534 (OMIM: 606148) fatty acid desaturase 1 [Homo (501 aa) initn: 2028 init1: 1030 opt: 1984 Z-score: 2613.8 bits: 493.0 E(85289): 7.5e-139 Smith-Waterman score: 1984; 63.4% identity (84.1% similar) in 440 aa overlap (11-444:64-501) 10 20 30 pF1KB4 MGKGGNQGEGAAEREVSVPT---FSWEEIQKHNLRTDRWL ::: .. :: :.:.:. ... .::: NP_037 WSPRTPSTRLTAPAGPARGVARPAMAPDPVAAETAAQGPTPRYFTWDEVAQRSGCEERWL 40 50 60 70 80 90 40 50 60 70 80 90 pF1KB4 VIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPE :::::::::.... .::::.:::.::::.:::: : ::: . .: :... ::::::.:: NP_037 VIDRKVYNISEFTRRHPGGSRVISHYAGQDATDPFVAFHINKGLVKKYMNSLLIGELSPE 100 110 120 130 140 150 100 110 120 130 140 150 pF1KB4 EPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNG .:: . ::...:..:: :: :.: :.:.:.::::::: : ::. :.. ::.:.. ::.. NP_037 QPSFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGTS 160 170 180 190 200 210 160 170 180 190 200 210 pF1KB4 WIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRH ..: :. : .:.. ::::::::::.:::::. :::::.:.::::::::: :.:::: : NP_037 FLPFLLCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMH 220 230 240 250 260 270 220 230 240 250 260 270 pF1KB4 FQHHAKPNIFHKDPDVNMLH--VFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLI :::::::: :.::::.:: : :.::. .: ::.: ::.::::::.:::::::: :. NP_037 FQHHAKPNCFRKDPDINM-HPFFFALGKILSVELGKQKKKYMPYNHQHKYFFLIGPPALL 280 290 300 310 320 330 280 290 300 310 320 330 pF1KB4 PMYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFI-RFLESHWF :.:::. :.. .: .:.:::::: ...:.:::.::.:. : : :.: : :: :::::.:: NP_037 PLYFQWYIFYFVIQRKKWVDLAWMITFYVRFFLTYVPLLG-LKAFLGLFFIVRFLESNWF 340 350 360 370 380 390 340 350 360 370 380 390 pF1KB4 VWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRH ::::::::: :.::.. :: :.:: :::::..: :::::::::::::::::::::::: NP_037 VWVTQMNHIPMHIDHDRNMDWVSTQLQATCNVHKSAFNDWFSGHLNFQIEHHLFPTMPRH 400 410 420 430 440 450 400 410 420 430 440 pF1KB4 NLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK : ::.::::.::::::::::: :::: :. :::.:::.::.::::::::. NP_037 NYHKVAPLVQSLCAKHGIEYQSKPLLSAFADIIHSLKESGQLWLDAYLHQ 460 470 480 490 500 >>XP_016873213 (OMIM: 606150) PREDICTED: fatty acid desa (491 aa) initn: 1822 init1: 986 opt: 1793 Z-score: 2362.0 bits: 446.4 E(85289): 8e-125 Smith-Waterman score: 1793; 63.2% identity (83.5% similar) in 389 aa overlap (56-444:104-491) 30 40 50 60 70 80 pF1KB4 IQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKF :.: :. . ::::::: ::.:: :: XP_016 PPLGALDLTLQDARLEAPQAPLLTDRDSASGSRGRGEALRDAQQDAFRAFHQDLNFVRKF 80 90 100 110 120 130 90 100 110 120 130 140 pF1KB4 LKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALES :.::::::::::::::: :....::::::...::::.:: .. .:: .::.::.:.: XP_016 LQPLLIGELAPEEPSQDGPLNAQLVEDFRALHQAAEDMKLFDASPTFFAFLLGHILAMEV 140 150 160 170 180 190 150 160 170 180 190 200 pF1KB4 IAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHL .::. .. .: ::.:. ..::.:: ::::. :::: :: :...: :::...:::.:.: XP_016 LAWLLIYLLGPGWVPSALAAFILAISQAQSWCLQHDLGHASIFKKSWWNHVAQKFVMGQL 200 210 220 230 240 250 210 220 230 240 250 260 pF1KB4 KGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEY :: ::.::: ::::::::::::::::::.. ::.::: . .:::::: .:::::.:: : XP_016 KGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPVFLLGE-SSVEYGKKKRRYLPYNQQHLY 260 270 280 290 300 310 270 280 290 300 310 320 pF1KB4 FFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNF ::::::::: . :. . . :.: .:.:: ::.:.: :::..:.::::. :.:::. XP_016 FFLIGPPLLTLVNFEVENLAYMLVCMQWADLLWAASFYARFFLSYLPFYGVPGVLLFFVA 320 330 340 350 360 370 330 340 350 360 370 380 pF1KB4 IRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEH .: ::::::::.:::::: :: .: .::: ::::.:::::: :.:..:::::::::::: XP_016 VRVLESHWFVWITQMNHIPKEIGHEKHRDWVSSQLAATCNVEPSLFTNWFSGHLNFQIEH 380 390 400 410 420 430 390 400 410 420 430 440 pF1KB4 HLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK :::: ::::: ..::::::::::::. :. ::.: ::.::.::::::: .:::::::. XP_016 HLFPRMPRHNYSRVAPLVKSLCAKHGLSYEVKPFLTALVDIVRSLKKSGDIWLDAYLHQ 440 450 460 470 480 490 >>XP_016873212 (OMIM: 606150) PREDICTED: fatty acid desa (500 aa) initn: 1799 init1: 810 opt: 1756 Z-score: 2313.0 bits: 437.3 E(85289): 4.3e-122 Smith-Waterman score: 1756; 61.6% identity (81.7% similar) in 398 aa overlap (56-444:104-500) 30 40 50 60 70 80 pF1KB4 IQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKF :.: :. . ::::::: ::.:: :: XP_016 PPLGALDLTLQDARLEAPQAPLLTDRDSASGSRGRGEALRDAQQDAFRAFHQDLNFVRKF 80 90 100 110 120 130 90 100 110 120 130 140 pF1KB4 LKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALES :.::::::::::::::: :....::::::...::::.:: .. .:: .::.::.:.: XP_016 LQPLLIGELAPEEPSQDGPLNAQLVEDFRALHQAAEDMKLFDASPTFFAFLLGHILAMEV 140 150 160 170 180 190 150 160 170 180 190 200 pF1KB4 IAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHL .::. .. .: ::.:. ..::.:: ::::. :::: :: :...: :::...:::.:.: XP_016 LAWLLIYLLGPGWVPSALAAFILAISQAQSWCLQHDLGHASIFKKSWWNHVAQKFVMGQL 200 210 220 230 240 250 210 220 230 240 250 260 pF1KB4 KGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEY :: ::.::: ::::::::::::::::::.. ::.::: . .:::::: .:::::.:: : XP_016 KGFSAHWWNFRHFQHHAKPNIFHKDPDVTVAPVFLLGE-SSVEYGKKKRRYLPYNQQHLY 260 270 280 290 300 310 270 280 290 300 310 320 pF1KB4 FFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNF ::::::::: . :. . . :.: .:.:: ::.:.: :::..:.::::. :.:::. XP_016 FFLIGPPLLTLVNFEVENLAYMLVCMQWADLLWAASFYARFFLSYLPFYGVPGVLLFFVA 320 330 340 350 360 370 330 340 350 360 370 380 pF1KB4 IRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEH .: ::::::::.:::::: :: .: .::: ::::.:::::: :.:..:::::::::::: XP_016 VRVLESHWFVWITQMNHIPKEIGHEKHRDWVSSQLAATCNVEPSLFTNWFSGHLNFQIEH 380 390 400 410 420 430 390 400 410 420 430 pF1KB4 H---------LFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKL . ::: ::::: ..::::::::::::. :. ::.: ::.::.::::::: . XP_016 QCQHTLPTPSLFPRMPRHNYSRVAPLVKSLCAKHGLSYEVKPFLTALVDIVRSLKKSGDI 440 450 460 470 480 490 440 pF1KB4 WLDAYLHK :::::::. XP_016 WLDAYLHQ 500 >>XP_011543324 (OMIM: 606148) PREDICTED: fatty acid desa (430 aa) initn: 1836 init1: 1030 opt: 1753 Z-score: 2310.1 bits: 436.6 E(85289): 6.2e-122 Smith-Waterman score: 1753; 64.8% identity (84.4% similar) in 378 aa overlap (70-444:55-430) 40 50 60 70 80 90 pF1KB4 DRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEP : : ::: . .: :... ::::::.::.: XP_011 PTRRITYRARGCRTCQRESYRHGPYLAQPKDPFVAFHINKGLVKKYMNSLLIGELSPEQP 30 40 50 60 70 80 100 110 120 130 140 150 pF1KB4 SQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWI : . ::...:..:: :: :.: :.:.:.::::::: : ::. :.. ::.:.. ::.... XP_011 SFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTLWVFGTSFL 90 100 110 120 130 140 160 170 180 190 200 210 pF1KB4 PTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQ : :. : .:.. ::::::::::.:::::. :::::.:.::::::::: :.:::: ::: XP_011 PFLLCAVLLSAVQAQAGWLQHDFGHLSVFSTSKWNHLLHHFVIGHLKGAPASWWNHMHFQ 150 160 170 180 190 200 220 230 240 250 260 270 pF1KB4 HHAKPNIFHKDPDVNMLH--VFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPM :::::: :.::::.:: : :.::. .: ::.: ::.::::::.:::::::: :.:. XP_011 HHAKPNCFRKDPDINM-HPFFFALGKILSVELGKQKKKYMPYNHQHKYFFLIGPPALLPL 210 220 230 240 250 260 280 290 300 310 320 330 pF1KB4 YFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFI-RFLESHWFVW :::. :.. .: .:.:::::: ...:.:::.::.:. : : :.: : :: :::::.:::: XP_011 YFQWYIFYFVIQRKKWVDLAWMITFYVRFFLTYVPLLG-LKAFLGLFFIVRFLESNWFVW 270 280 290 300 310 320 340 350 360 370 380 390 pF1KB4 VTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNL ::::::: :.::.. :: :.:: :::::..: ::::::::::::::::::::::::: XP_011 VTQMNHIPMHIDHDRNMDWVSTQLQATCNVHKSAFNDWFSGHLNFQIEHHLFPTMPRHNY 330 340 350 360 370 380 400 410 420 430 440 pF1KB4 HKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK ::.::::.::::::::::: :::: :. :::.:::.::.::::::::. XP_011 HKVAPLVQSLCAKHGIEYQSKPLLSAFADIIHSLKESGQLWLDAYLHQ 390 400 410 420 430 >>NP_683725 (OMIM: 250790,613218) cytochrome b5 isoform (134 aa) initn: 224 init1: 185 opt: 210 Z-score: 282.2 bits: 59.7 E(85289): 5.6e-09 Smith-Waterman score: 210; 35.6% identity (68.3% similar) in 101 aa overlap (11-110:2-101) 10 20 30 40 50 60 pF1KB4 MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVI : . . .: .. ::::::: . ::.. .:::..::. .::::..:. NP_683 MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVL 10 20 30 40 50 70 80 90 100 110 pF1KB4 GHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEE-PSQDHGKNSKITEDFRALRKT . :: :::. :. . . . .. : ..:::: :.. :. .. .. :: NP_683 REQAGGDATENFEDVGHSTD-AREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWW 60 70 80 90 100 110 120 130 140 150 160 170 pF1KB4 AEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQ NP_683 TNWVIPAISAVAVALMYRLYMAED 120 130 444 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Sat Nov 5 06:01:41 2016 done: Sat Nov 5 06:01:42 2016 Total Scan time: 8.100 Total Display time: 0.060 Function used was FASTA [36.3.4 Apr, 2011]