Restriction map for
pF1KB4708
(Circular) (Six-base) MAPSORT of: pF1KB4708.seq Check: 5030 from: 1 to: 4445
pF1KB4708 4445 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1204 1204
Size: 4445
AccI GT'mk_AC
Cuts at: 668 1483 668
Size: 815 3630
Acc65I G'GTAC_C
Cuts at: 1461 1461
Size: 4445
AclI AA'CG_TT
Cuts at: 4285 4285
Size: 4445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 1417 2270 2702 3449 3717 153
Size: 1264 853 432 747 268 881
Fragments arranged by size:
1264 881 853 747 432 268
AflIII A'CryG_T
Cuts at: 497 818 1797 2901 3878 497
Size: 321 979 1104 977 1064
Fragments arranged by size:
1104 1064 979 977 321
AgeI A'CCGG_T
Cuts at: 2833 2833
Size: 4445
AlwNI CAG_nnn'CTG
Cuts at: 1750 3317 1750
Size: 1567 2878
ApaI G_GGCC'C
Cuts at: 453 1374 453
Size: 921 3524
ApaLI G'TGCA_C
Cuts at: 3215 3215
Size: 4445
ApoI r'AATT_y
Cuts at: 346 703 1449 3798 4054 346
Size: 357 746 2349 256 737
Fragments arranged by size:
2349 746 737 357 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4445
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
AvaI C'yCGr_G
Cuts at: 267 1465 267
Size: 1198 3247
BamHI G'GATC_C
Cuts at: 795 1470 795
Size: 675 3770
BanI G'GyrC_C
Cuts at: 159 727 1461 1875 2123 2158 159
Size: 568 734 414 248 35 2446
Fragments arranged by size:
2446 734 568 414 248 35
BanII G_rGCy'C
Cuts at: 404 453 1065 1374 1459 2489 404
Size: 49 612 309 85 1030 2360
Fragments arranged by size:
2360 1030 612 309 85 49
BbeI G_GCGC'C
Cuts at: 1879 2127 1879
Size: 248 4197
BbsI GAAGACnn'nnnn_
Cuts at: 466 466
Size: 4445
BbvCI CC'TCA_GC
Cuts at: 467 467
Size: 4445
BciVI GTATCCnnnnn_n'
Cuts at: 2343 3104 4445 2343
Size: 761 1341 2343
Fragments arranged by size:
2343 1341 761
BclI T'GATC_A
Cuts at: 1965 1965
Size: 4445
BglII A'GATC_T
Cuts at: 1960 3997 1960
Size: 2037 2408
BlpI GC'TnA_GC
Cuts at: 1557 1557
Size: 4445
Bme1580I G_kGCm'C
Cuts at: 164 293 453 1374 2070 2163 3219 164
Size: 129 160 921 696 93 1056 1390
Fragments arranged by size:
1390 1056 921 696 160 129 93
BmrI ACTGGGnnnn_n'
Cuts at: 993 1657 1738 2073 4217 993
Size: 664 81 335 2144 1221
Fragments arranged by size:
2144 1221 664 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2812 2812
Size: 4445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 556 1114 320
Size: 236 558 3651
Fragments arranged by size:
3651 558 236
Bpu10I CC'TnA_GC
Cuts at: 467 467
Size: 4445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1621 2500 2992 3290 1621
Size: 879 492 298 2776
Fragments arranged by size:
2776 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4149 4149
Size: 4445
BsaAI yAC'GTr
Cuts at: 498 819 1798 498
Size: 321 979 3145
Fragments arranged by size:
3145 979 321
BsaBI GATnn'nnATC
Cuts at: 6 1959 3996 6
Size: 1953 2037 455
Fragments arranged by size:
2037 1953 455
BsaHI Gr'CG_yC
Cuts at: 1424 1876 2124 2826 4322 1424
Size: 452 248 702 1496 1547
Fragments arranged by size:
1547 1496 702 452 248
BsaWI w'CCGG_w
Cuts at: 1019 1634 2155 2833 3107 3254 3665 1019
Size: 615 521 678 274 147 411 1799
Fragments arranged by size:
1799 678 615 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 412 499 502 193
Size: 219 87 3 4136
Fragments arranged by size:
4136 219 87 3
BseYI C'CCAG_C
Cuts at: 173 403 1870 2628 3205 3701 173
Size: 230 1467 758 577 496 917
Fragments arranged by size:
1467 917 758 577 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 799
Size: 4445
BsiEI CG_ry'CG
Cuts at: 104 1987 2033 3241 104
Size: 1883 46 1208 1308
Fragments arranged by size:
1883 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1262 1459 2237 2427 3219 1262
Size: 197 778 190 792 2488
Fragments arranged by size:
2488 792 778 197 190
Bsp1286I G_dGCh'C
Cuts at: 164 293 404 453 1065 1262 1374 1459 2070
Size: 129 111 49 612 197 112 85 611
Cuts at: 2070 2163 2237 2427 2489 3219 164
Size: 93 74 190 62 730 1390
Fragments arranged by size:
1390 730 612 611 197 190 129 112
111 93 85 74 62 49
BspEI T'CCGG_A
Cuts at: 3665 3665
Size: 4445
BspHI T'CATG_A
Cuts at: 959 3621 959
Size: 2662 1783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 884 1204 1495 1709 2011 2392 206
Size: 678 320 291 214 302 381 2259
Fragments arranged by size:
2259 678 381 320 302 291 214
BsrBI CCG'CTC
Cuts at: 50 2737 2791 4275 4440 50
Size: 2687 54 1484 165 55
Fragments arranged by size:
2687 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 542 579 2357 542
Size: 37 1778 2630
Fragments arranged by size:
2630 1778 37
BsrFI r'CCGG_y
Cuts at: 2443 2624 2833 2443
Size: 181 209 4055
Fragments arranged by size:
4055 209 181
BssSI C'ACGA_G
Cuts at: 2716 3074 2716
Size: 358 4087
BstAPI GCAn_nnn'nTGC
Cuts at: 198 316 1822 198
Size: 118 1506 2821
Fragments arranged by size:
2821 1506 118
BstBI TT'CG_AA
Cuts at: 2806 2806
Size: 4445
BstYI r'GATC_y
Cuts at: 1 795 1470 1937 1960 2295 2541 3553 3639
Size: 794 675 467 23 335 246 1012 86
Cuts at: 3639 3651 3997 1
Size: 12 346 449
Fragments arranged by size:
1012 794 675 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 454 454
Size: 4445
BtgI C'CryG_G
Cuts at: 107 414 107
Size: 307 4138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 513 736 2395 2576 2739 513
Size: 223 1659 181 163 2219
Fragments arranged by size:
2219 1659 223 181 163
BtsI GCAGTG_nn'
Cuts at: 1688 1688
Size: 4445
DraI TTT'AAA
Cuts at: 1444 1444
Size: 4445
DrdI GACnn_nn'nnGTC
Cuts at: 2151 3009 2151
Size: 858 3587
EaeI y'GGCC_r
Cuts at: 654 714 2030 2204 2595 2622 3967 654
Size: 60 1316 174 391 27 1345 1132
Fragments arranged by size:
1345 1316 1132 391 174 60 27
EagI C'GGCC_G
Cuts at: 2030 2030
Size: 4445
EarI CTCTTCn'nnn_
Cuts at: 1273 2468 2678 1273
Size: 1195 210 3040
Fragments arranged by size:
3040 1195 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2653 2963 3109 4255 2653
Size: 310 146 1146 2843
Fragments arranged by size:
2843 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1457 1457
Size: 4445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 320 556 1114 1417 2270 2702 3449 3717
Size: 167 236 558 303 853 432 747 268
Cuts at: 3717 153
Size: 881
Fragments arranged by size:
881 853 747 558 432 303 268 236
167
EcoO109I rG'GnC_Cy
Cuts at: 450 1370 1371 1391 1584 450
Size: 920 1 20 193 3311
Fragments arranged by size:
3311 920 193 20 1
EcoRI G'AATT_C
Cuts at: 346 1449 346
Size: 1103 3342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2464 2464
Size: 4445
FspI TGC'GCA
Cuts at: 202 2226 202
Size: 2024 2421
HaeII r_GCGC'y
Cuts at: 1879 2127 3149 4128 1879
Size: 248 1022 979 2196
Fragments arranged by size:
2196 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2287 2317 2319 2349 2287
Size: 30 2 30 4383
Fragments arranged by size:
4383 30 30 2
HincII GTy'rAC
Cuts at: 1484 1484
Size: 4445
KasI G'GCGC_C
Cuts at: 1875 2123 1875
Size: 248 4197
KpnI G_GTAC'C
Cuts at: 1465 1465
Size: 4445
MluI A'CGCG_T
Cuts at: 3878 3878
Size: 4445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3300 3116
Size: 184 4261
MscI TGG'CCA
Cuts at: 656 716 2206 656
Size: 60 1490 2895
Fragments arranged by size:
2895 1490 60
MslI CAynn'nnrTG
Cuts at: 650 934 964 1123 1138 2434 2561 650
Size: 284 30 159 15 1296 127 2534
Fragments arranged by size:
2534 1296 284 159 127 30 15
MspA1I CmG'CkG
Cuts at: 173 279 924 1185 1311 1556 1870 2230 3243
Size: 106 645 261 126 245 314 360 1013
Cuts at: 3243 3488 173
Size: 245 1130
Fragments arranged by size:
1130 1013 645 360 314 261 245 245
126 106
NaeI GCC'GGC
Cuts at: 2626 2626
Size: 4445
NarI GG'CG_CC
Cuts at: 1876 2124 1876
Size: 248 4197
NcoI C'CATG_G
Cuts at: 107 107
Size: 4445
NgoMIV G'CCGG_C
Cuts at: 2624 2624
Size: 4445
NspI r_CATG'y
Cuts at: 813 1498 1998 2905 813
Size: 685 500 907 2353
Fragments arranged by size:
2353 907 685 500
PciI A'CATG_T
Cuts at: 2901 2901
Size: 4445
PflMI CCAn_nnn'nTGG
Cuts at: 743 743
Size: 4445
PfoI T'CCnGG_A
Cuts at: 1091 1091
Size: 4445
PmeI GTTT'AAAC
Cuts at: 1444 1444
Size: 4445
PmlI CAC'GTG
Cuts at: 498 819 498
Size: 321 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4413
PpuMI rG'GwC_Cy
Cuts at: 1391 1391
Size: 4445
PspOMI G'GGCC_C
Cuts at: 449 1370 449
Size: 921 3524
PstI C_TGCA'G
Cuts at: 1492 2177 1492
Size: 685 3760
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4445
PvuII CAG'CTG
Cuts at: 173 1185 1870 2230 173
Size: 1012 685 360 2388
Fragments arranged by size:
2388 1012 685 360
RsrII CG'GwC_CG
Cuts at: 2640 2640
Size: 4445
SacI G_AGCT'C
Cuts at: 1459 1459
Size: 4445
SalI G'TCGA_C
Cuts at: 1482 1482
Size: 4445
SapI GCTCTTCn'nnn_
Cuts at: 2468 2678 2468
Size: 210 4235
SbfI CC_TGCA'GG
Cuts at: 1492 1492
Size: 4445
ScaI AGT'ACT
Cuts at: 3962 3962
Size: 4445
SfcI C'TryA_G
Cuts at: 33 669 1488 2173 3166 3357 33
Size: 636 819 685 993 191 1121
Fragments arranged by size:
1121 993 819 685 636 191
SfoI GGC'GCC
Cuts at: 1877 2125 1877
Size: 248 4197
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4445
SmaI CCC'GGG
Cuts at: 269 1467 269
Size: 1198 3247
SmlI C'TyrA_G
Cuts at: 1600 2515 3007 3269 1600
Size: 915 492 262 2776
Fragments arranged by size:
2776 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4445
SphI G_CATG'C
Cuts at: 1498 1998 1498
Size: 500 3945
StuI AGG'CCT
Cuts at: 1159 1159
Size: 4445
StyI C'CwwG_G
Cuts at: 107 1579 1933 107
Size: 1472 354 2619
Fragments arranged by size:
2619 1472 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 975 2028 2161 2620 2829 975
Size: 1053 133 459 209 2591
Fragments arranged by size:
2591 1053 459 209 133
TatI w'GTAC_w
Cuts at: 3960 3960
Size: 4445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 4413
Tth111I GACn'n_nGTC
Cuts at: 2242 2242
Size: 4445
XbaI T'CTAG_A
Cuts at: 1476 1476
Size: 4445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1200 1940 1200
Size: 740 3705
XmaI C'CCGG_G
Cuts at: 267 1465 267
Size: 1198 3247
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 4445
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI