Restriction map for
pF1KB4713
(Circular) (Six-base) MAPSORT of: pF1KB4713.seq Check: 9213 from: 1 to: 5441
pF1KB4713 5441 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 346 346
Size: 5441
AccI GT'mk_AC
Cuts at: 2479 2479
Size: 5441
Acc65I G'GTAC_C
Cuts at: 2457 2457
Size: 5441
AclI AA'CG_TT
Cuts at: 5281 5281
Size: 5441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 827 932 1097 1790 3266 3698 4445 4713
Size: 355 105 165 693 1476 432 747 268
Cuts at: 4713 472
Size: 1200
Fragments arranged by size:
1476 1200 747 693 432 355 268 165
105
AflII C'TTAA_G
Cuts at: 2167 2167
Size: 5441
AflIII A'CryG_T
Cuts at: 2793 3897 4874 2793
Size: 1104 977 3360
Fragments arranged by size:
3360 1104 977
AgeI A'CCGG_T
Cuts at: 3829 3829
Size: 5441
AleI CACnn'nnGTG
Cuts at: 349 637 349
Size: 288 5153
AlwNI CAG_nnn'CTG
Cuts at: 838 2202 2746 4313 838
Size: 1364 544 1567 1966
Fragments arranged by size:
1966 1567 1364 544
ApaI G_GGCC'C
Cuts at: 273 307 1816 273
Size: 34 1509 3898
Fragments arranged by size:
3898 1509 34
ApaLI G'TGCA_C
Cuts at: 4211 4211
Size: 5441
ApoI r'AATT_y
Cuts at: 482 2218 2445 4794 5050 482
Size: 1736 227 2349 256 873
Fragments arranged by size:
2349 1736 873 256 227
AseI AT'TA_AT
Cuts at: 20 20
Size: 5441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
AvaI C'yCGr_G
Cuts at: 157 252 272 300 712 745 1258 1708 1779
Size: 95 20 28 412 33 513 450 71
Cuts at: 1779 2461 157
Size: 682 3137
Fragments arranged by size:
3137 682 513 450 412 95 71 33
28 20
BamHI G'GATC_C
Cuts at: 123 2466 123
Size: 2343 3098
BanI G'GyrC_C
Cuts at: 176 228 262 1834 2457 2871 3119 3154 176
Size: 52 34 1572 623 414 248 35 2463
Fragments arranged by size:
2463 1572 623 414 248 52 35 34
BanII G_rGCy'C
Cuts at: 248 273 307 526 930 1505 1549 1697 1734
Size: 25 34 219 404 575 44 148 37
Cuts at: 1734 1816 2455 3485 248
Size: 82 639 1030 2204
Fragments arranged by size:
2204 1030 639 575 404 219 148 82
44 37 34 25
BbeI G_GCGC'C
Cuts at: 232 266 1838 2875 3123 232
Size: 34 1572 1037 248 2550
Fragments arranged by size:
2550 1572 1037 248 34
BbsI GAAGACnn'nnnn_
Cuts at: 1009 1085 1781 1009
Size: 76 696 4669
Fragments arranged by size:
4669 696 76
BbvCI CC'TCA_GC
Cuts at: 2432 2432
Size: 5441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1615 1649 1615
Size: 34 5407
BciVI GTATCCnnnnn_n'
Cuts at: 3339 4100 5441 3339
Size: 761 1341 3339
Fragments arranged by size:
3339 1341 761
BclI T'GATC_A
Cuts at: 1175 2961 1175
Size: 1786 3655
BglI GCCn_nnn'nGGC
Cuts at: 226 1418 1531 1644 1649 1987 226
Size: 1192 113 113 5 338 3680
Fragments arranged by size:
3680 1192 338 113 113 5
BglII A'GATC_T
Cuts at: 647 2956 4993 647
Size: 2309 2037 1095
Fragments arranged by size:
2309 2037 1095
BlpI GC'TnA_GC
Cuts at: 2553 2553
Size: 5441
Bme1580I G_kGCm'C
Cuts at: 273 307 1816 3066 3159 4215 273
Size: 34 1509 1250 93 1056 1499
Fragments arranged by size:
1509 1499 1250 1056 93 34
BmgBI CAC'GTC
Cuts at: 507 507
Size: 5441
BmrI ACTGGGnnnn_n'
Cuts at: 159 1950 2653 2734 3069 5213 159
Size: 1791 703 81 335 2144 387
Fragments arranged by size:
2144 1791 703 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1581 3808 1581
Size: 2227 3214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1742 1756 1742
Size: 14 5427
Bpu10I CC'TnA_GC
Cuts at: 172 2432 172
Size: 2260 3181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 2617 3496 3988 4286 2116
Size: 501 879 492 298 3271
Fragments arranged by size:
3271 879 501 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5145 5145
Size: 5441
BsaAI yAC'GTr
Cuts at: 1416 2154 2794 1416
Size: 738 640 4063
Fragments arranged by size:
4063 738 640
BsaBI GATnn'nnATC
Cuts at: 6 2955 4992 6
Size: 2949 2037 455
Fragments arranged by size:
2949 2037 455
BsaHI Gr'CG_yC
Cuts at: 208 229 263 283 1523 1616 1747 1835 2872
Size: 21 34 20 1240 93 131 88 1037
Cuts at: 2872 3120 3822 5318 208
Size: 248 702 1496 331
Fragments arranged by size:
1496 1240 1037 702 331 248 131 93
88 34 21 20
BsaWI w'CCGG_w
Cuts at: 1276 2630 3151 3829 4103 4250 4661 1276
Size: 1354 521 678 274 147 411 2056
Fragments arranged by size:
2056 1354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 873 903 873
Size: 30 5411
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 730 920 1489 1518 1555 1621 1642 2193
Size: 186 190 569 29 37 66 21 551
Cuts at: 2193 544
Size: 3792
Fragments arranged by size:
3792 569 551 190 186 66 37 29
21
BseYI C'CCAG_C
Cuts at: 238 247 534 1294 1582 1697 2866 3624 4201
Size: 9 287 760 288 115 1169 758 577
Cuts at: 4201 4697 238
Size: 496 982
Fragments arranged by size:
1169 982 760 758 577 496 288 287
115 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2071 2071
Size: 5441
BsiEI CG_ry'CG
Cuts at: 104 1234 2983 3029 4237 104
Size: 1130 1749 46 1208 1308
Fragments arranged by size:
1749 1308 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 1505 2455 3233 3423 4215 1505
Size: 950 778 190 792 2731
Fragments arranged by size:
2731 950 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1718 1738 1718
Size: 20 5421
Bsp1286I G_dGCh'C
Cuts at: 248 273 307 526 930 1505 1549 1697 1734
Size: 25 34 219 404 575 44 148 37
Cuts at: 1734 1816 2455 3066 3159 3233 3423 3485 4215
Size: 82 639 611 93 74 190 62 730
Cuts at: 4215 248
Size: 1474
Fragments arranged by size:
1474 730 639 611 575 404 219 190
148 93 82 74 62 44 37 34
25
BspEI T'CCGG_A
Cuts at: 1276 4661 1276
Size: 3385 2056
BspHI T'CATG_A
Cuts at: 4617 4617
Size: 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 2491 2705 3007 3388 346
Size: 2145 214 302 381 2399
Fragments arranged by size:
2399 2145 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3733 3787 5271 5436 50
Size: 3683 54 1484 165 55
Fragments arranged by size:
3683 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3353 3353
Size: 5441
BsrFI r'CCGG_y
Cuts at: 151 225 295 1233 1643 3439 3620 3829 151
Size: 74 70 938 410 1796 181 209 1763
Fragments arranged by size:
1796 1763 938 410 209 181 74 70
BssSI C'ACGA_G
Cuts at: 581 1309 3712 4070 581
Size: 728 2403 358 1952
Fragments arranged by size:
2403 1952 728 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2818 2818
Size: 5441
BstBI TT'CG_AA
Cuts at: 3802 3802
Size: 5441
BstXI CCAn_nnnn'nTGG
Cuts at: 351 372 1302 1590 351
Size: 21 930 288 4202
Fragments arranged by size:
4202 930 288 21
BstYI r'GATC_y
Cuts at: 1 123 647 1093 2466 2933 2956 3291 3537
Size: 122 524 446 1373 467 23 335 246
Cuts at: 3537 4549 4635 4647 4993 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1373 1012 524 467 449 446 346 335
246 122 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 455 527 1392 1468 455
Size: 72 865 76 4428
Fragments arranged by size:
4428 865 76 72
BtgI C'CryG_G
Cuts at: 107 329 371 632 1228 1367 1373 1759 107
Size: 222 42 261 596 139 6 386 3789
Fragments arranged by size:
3789 596 386 261 222 139 42 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3391 3572 3735 3391
Size: 181 163 5097
Fragments arranged by size:
5097 181 163
BtsI GCAGTG_nn'
Cuts at: 1846 2054 2684 1846
Size: 208 630 4603
Fragments arranged by size:
4603 630 208
DraI TTT'AAA
Cuts at: 2440 2440
Size: 5441
DrdI GACnn_nn'nnGTC
Cuts at: 3147 4005 3147
Size: 858 4583
EaeI y'GGCC_r
Cuts at: 110 149 501 1231 1247 1370 1410 1655 1802
Size: 39 352 730 16 123 40 245 147
Cuts at: 1802 1994 3026 3200 3591 3618 4963 110
Size: 192 1032 174 391 27 1345 588
Fragments arranged by size:
1345 1032 730 588 391 352 245 192
174 147 123 40 39 27 16
EagI C'GGCC_G
Cuts at: 1231 3026 1231
Size: 1795 3646
EarI CTCTTCn'nnn_
Cuts at: 1948 2191 2266 3464 3674 1948
Size: 243 75 1198 210 3715
Fragments arranged by size:
3715 1198 243 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 3959 4105 5251 3649
Size: 310 146 1146 3839
Fragments arranged by size:
3839 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1503 2453 1503
Size: 950 4491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 827 932 1097 1742 1756 1790 3266 3698
Size: 355 105 165 645 14 34 1476 432
Cuts at: 3698 4445 4713 472
Size: 747 268 1200
Fragments arranged by size:
1476 1200 747 645 432 355 268 165
105 34 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1471 2110 2194 1471
Size: 639 84 4718
Fragments arranged by size:
4718 639 84
EcoO109I rG'GnC_Cy
Cuts at: 161 269 304 1362 1650 1812 1813 1828 2199
Size: 108 35 1058 288 162 1 15 371
Cuts at: 2199 2580 161
Size: 381 3022
Fragments arranged by size:
3022 1058 381 371 288 162 108 35
15 1
EcoRI G'AATT_C
Cuts at: 482 2445 482
Size: 1963 3478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2103 3460 2103
Size: 1357 4084
FseI GG_CCGG'CC
Cuts at: 155 155
Size: 5441
FspI TGC'GCA
Cuts at: 3222 3222
Size: 5441
HaeII r_GCGC'y
Cuts at: 232 266 1589 1838 2258 2420 2875 3123 4145
Size: 34 1323 249 420 162 455 248 1022
Cuts at: 4145 5124 232
Size: 979 549
Fragments arranged by size:
1323 1022 979 549 455 420 249 248
162 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 564 596 892 924 1339 1371 1513 1545 1669
Size: 32 296 32 415 32 142 32 124
Cuts at: 1669 1701 3283 3313 3315 3345 564
Size: 32 1582 30 2 30 2660
Fragments arranged by size:
2660 1582 415 296 142 124 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2480 2480
Size: 5441
KasI G'GCGC_C
Cuts at: 228 262 1834 2871 3119 228
Size: 34 1572 1037 248 2550
Fragments arranged by size:
2550 1572 1037 248 34
KpnI G_GTAC'C
Cuts at: 2461 2461
Size: 5441
MluI A'CGCG_T
Cuts at: 4874 4874
Size: 5441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1811 4112 4296 1811
Size: 2301 184 2956
Fragments arranged by size:
2956 2301 184
MscI TGG'CCA
Cuts at: 503 1412 3202 503
Size: 909 1790 2742
Fragments arranged by size:
2742 1790 909
MslI CAynn'nnrTG
Cuts at: 349 637 2290 2378 3430 3557 349
Size: 288 1653 88 1052 127 2233
Fragments arranged by size:
2233 1653 1052 288 127 88
MspA1I CmG'CkG
Cuts at: 147 1245 1375 2552 2866 3226 4239 4484 147
Size: 1098 130 1177 314 360 1013 245 1104
Fragments arranged by size:
1177 1104 1098 1013 360 314 245 130
NaeI GCC'GGC
Cuts at: 153 227 297 1645 3622 153
Size: 74 70 1348 1977 1972
Fragments arranged by size:
1977 1972 1348 74 70
NarI GG'CG_CC
Cuts at: 229 263 1835 2872 3120 229
Size: 34 1572 1037 248 2550
Fragments arranged by size:
2550 1572 1037 248 34
NcoI C'CATG_G
Cuts at: 107 329 371 1367 107
Size: 222 42 996 4181
Fragments arranged by size:
4181 996 222 42
NgoMIV G'CCGG_C
Cuts at: 151 225 295 1643 3620 151
Size: 74 70 1348 1977 1972
Fragments arranged by size:
1977 1972 1348 74 70
NspI r_CATG'y
Cuts at: 2494 2994 3901 2494
Size: 500 907 4034
Fragments arranged by size:
4034 907 500
PasI CC'CwG_GG
Cuts at: 1143 1816 1143
Size: 673 4768
PciI A'CATG_T
Cuts at: 3897 3897
Size: 5441
PflMI CCAn_nnn'nTGG
Cuts at: 870 1114 2202 2355 870
Size: 244 1088 153 3956
Fragments arranged by size:
3956 1088 244 153
PfoI T'CCnGG_A
Cuts at: 1601 2195 1601
Size: 594 4847
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5441
PmlI CAC'GTG
Cuts at: 1416 1416
Size: 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 4580
Size: 32 5409
PpuMI rG'GwC_Cy
Cuts at: 161 1362 1828 2199 161
Size: 1201 466 371 3403
Fragments arranged by size:
3403 1201 466 371
PshAI GACnn'nnGTC
Cuts at: 1615 1615
Size: 5441
PspOMI G'GGCC_C
Cuts at: 269 303 1812 269
Size: 34 1509 3898
Fragments arranged by size:
3898 1509 34
PspXI vC'TCGA_Gb
Cuts at: 1779 1779
Size: 5441
PstI C_TGCA'G
Cuts at: 627 1137 2147 2488 3173 627
Size: 510 1010 341 685 2895
Fragments arranged by size:
2895 1010 685 510 341
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5441
PvuII CAG'CTG
Cuts at: 1245 2866 3226 1245
Size: 1621 360 3460
Fragments arranged by size:
3460 1621 360
RsrII CG'GwC_CG
Cuts at: 1279 3636 1279
Size: 2357 3084
SacI G_AGCT'C
Cuts at: 1505 2455 1505
Size: 950 4491
SacII CC_GC'GG
Cuts at: 1376 1376
Size: 5441
SalI G'TCGA_C
Cuts at: 2478 2478
Size: 5441
SanDI GG'GwC_CC
Cuts at: 161 161
Size: 5441
SapI GCTCTTCn'nnn_
Cuts at: 1948 2191 3464 3674 1948
Size: 243 1273 210 3715
Fragments arranged by size:
3715 1273 243 210
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5441
ScaI AGT'ACT
Cuts at: 4958 4958
Size: 5441
SexAI A'CCwGG_T
Cuts at: 2201 2201
Size: 5441
SfcI C'TryA_G
Cuts at: 33 623 1133 2143 2315 2484 3169 4162 4353
Size: 590 510 1010 172 169 685 993 191
Cuts at: 4353 33
Size: 1121
Fragments arranged by size:
1121 1010 993 685 590 510 191 172
169
SfiI GGCCn_nnn'nGGCC
Cuts at: 1987 1987
Size: 5441
SfoI GGC'GCC
Cuts at: 230 264 1836 2873 3121 230
Size: 34 1572 1037 248 2550
Fragments arranged by size:
2550 1572 1037 248 34
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
SgrAI Cr'CCGG_yG
Cuts at: 225 225
Size: 5441
SmaI CCC'GGG
Cuts at: 159 254 274 2463 159
Size: 95 20 2189 3137
Fragments arranged by size:
3137 2189 95 20
SmlI C'TyrA_G
Cuts at: 1779 2095 2167 2596 3511 4003 4265 1779
Size: 316 72 429 915 492 262 2955
Fragments arranged by size:
2955 915 492 429 316 262 72
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5441
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4941
StuI AGG'CCT
Cuts at: 685 2083 685
Size: 1398 4043
StyI C'CwwG_G
Cuts at: 107 329 371 1367 2575 2929 107
Size: 222 42 996 1208 354 2619
Fragments arranged by size:
2619 1208 996 354 222 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3024 3157 3616 3825 3024
Size: 133 459 209 4640
Fragments arranged by size:
4640 459 209 133
TatI w'GTAC_w
Cuts at: 1457 2129 4956 1457
Size: 672 2827 1942
Fragments arranged by size:
2827 1942 672
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 400 432 873 905 4958 4990 400
Size: 32 441 32 4053 32 851
Fragments arranged by size:
4053 851 441 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1048 3238 1048
Size: 2190 3251
XbaI T'CTAG_A
Cuts at: 2472 2472
Size: 5441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 2936 255
Size: 2681 2760
XhoI C'TCGA_G
Cuts at: 1779 1779
Size: 5441
XmaI C'CCGG_G
Cuts at: 157 252 272 2461 157
Size: 95 20 2189 3137
Fragments arranged by size:
3137 2189 95 20
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PsiI PsrI
SnaBI SrfI SspI SwaI XmnI ZraI