Restriction map for
pF1KB4740
(Circular) (Six-base) MAPSORT of: pF1KB4740.seq Check: 2377 from: 1 to: 5723
pF1KB4740 5723 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 885 2439 2626 885
Size: 1554 187 3982
Fragments arranged by size:
3982 1554 187
AatII G_ACGT'C
Cuts at: 871 871
Size: 5723
AccI GT'mk_AC
Cuts at: 2761 2761
Size: 5723
Acc65I G'GTAC_C
Cuts at: 2506 2739 2506
Size: 233 5490
AclI AA'CG_TT
Cuts at: 5563 5563
Size: 5723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 965 1248 2188 3548 3980 4333 4995 689
Size: 276 283 940 1360 432 353 662 1417
Fragments arranged by size:
1417 1360 940 662 432 353 283 276
AflIII A'CryG_T
Cuts at: 1050 3075 4875 5156 1050
Size: 2025 1800 281 1617
Fragments arranged by size:
2025 1800 1617 281
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 5723
AhdI GACnn_n'nnGTC
Cuts at: 2040 2040
Size: 5723
AlwNI CAG_nnn'CTG
Cuts at: 835 991 3028 4466 835
Size: 156 2037 1438 2092
Fragments arranged by size:
2092 2037 1438 156
ApaI G_GGCC'C
Cuts at: 1324 1324
Size: 5723
ApaLI G'TGCA_C
Cuts at: 4561 4561
Size: 5723
ApoI r'AATT_y
Cuts at: 2727 5076 5332 2727
Size: 2349 256 3118
Fragments arranged by size:
3118 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5723
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
AvaI C'yCGr_G
Cuts at: 1133 1536 2249 2743 1133
Size: 403 713 494 4113
Fragments arranged by size:
4113 713 494 403
BamHI G'GATC_C
Cuts at: 1407 1527 2480 2748 1407
Size: 120 953 268 4382
Fragments arranged by size:
4382 953 268 120
BanI G'GyrC_C
Cuts at: 397 641 758 2506 2739 3153 3401 3436 397
Size: 244 117 1748 233 414 248 35 2684
Fragments arranged by size:
2684 1748 414 248 244 233 117 35
BanII G_rGCy'C
Cuts at: 295 1324 2737 3767 295
Size: 1029 1413 1030 2251
Fragments arranged by size:
2251 1413 1030 1029
BbeI G_GCGC'C
Cuts at: 3157 3405 3157
Size: 248 5475
BbsI GAAGACnn'nnnn_
Cuts at: 277 1280 277
Size: 1003 4720
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1923 1957 1923
Size: 34 5689
BciVI GTATCCnnnnn_n'
Cuts at: 3621 4677 5723 3621
Size: 1056 1046 3621
Fragments arranged by size:
3621 1056 1046
BclI T'GATC_A
Cuts at: 3243 3243
Size: 5723
BglI GCCn_nnn'nGGC
Cuts at: 791 940 791
Size: 149 5574
BglII A'GATC_T
Cuts at: 3238 5275 3238
Size: 2037 3686
BlpI GC'TnA_GC
Cuts at: 2835 2835
Size: 5723
Bme1580I G_kGCm'C
Cuts at: 576 1324 3348 3441 4565 576
Size: 748 2024 93 1124 1734
Fragments arranged by size:
2024 1734 1124 748 93
BmgBI CAC'GTC
Cuts at: 1051 1051
Size: 5723
BmrI ACTGGGnnnn_n'
Cuts at: 251 284 1392 2152 2935 3016 3351 5495 251
Size: 33 1108 760 783 81 335 2144 479
Fragments arranged by size:
2144 1108 783 760 479 335 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4090 4090
Size: 5723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 594 614 1091 1228 2059 2131 2329 2362
Size: 210 20 477 137 831 72 198 33
Cuts at: 2362 384
Size: 3745
Fragments arranged by size:
3745 831 477 210 198 137 72 33
20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 399 909 2899 3778 4492 4790 399
Size: 510 1990 879 714 298 1332
Fragments arranged by size:
1990 1332 879 714 510 298
BsaI GGTCTCn'nnnn_
Cuts at: 256 2013 2689 5427 256
Size: 1757 676 2738 552
Fragments arranged by size:
2738 1757 676 552
BsaAI yAC'GTr
Cuts at: 231 1203 1374 1650 3076 231
Size: 972 171 276 1426 2878
Fragments arranged by size:
2878 1426 972 276 171
BsaBI GATnn'nnATC
Cuts at: 6 3237 5274 6
Size: 3231 2037 455
Fragments arranged by size:
3231 2037 455
BsaHI Gr'CG_yC
Cuts at: 868 3154 3402 4104 5600 868
Size: 2286 248 702 1496 991
Fragments arranged by size:
2286 1496 991 702 248
BsaWI w'CCGG_w
Cuts at: 2503 2912 3433 4111 4522 4669 4943 2503
Size: 409 521 678 411 147 274 3283
Fragments arranged by size:
3283 678 521 411 409 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 965 995 1873 1903 965
Size: 30 878 30 4785
Fragments arranged by size:
4785 878 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1774 1881 2215 2707 1774
Size: 107 334 492 4790
Fragments arranged by size:
4790 492 334 107
BseYI C'CCAG_C
Cuts at: 652 720 930 958 984 1112 1337 3148 3906
Size: 68 210 28 26 128 225 1811 758
Cuts at: 3906 4571 4979 652
Size: 665 408 1396
Fragments arranged by size:
1811 1396 758 665 408 225 210 128
68 28 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 866 866
Size: 5723
BsiEI CG_ry'CG
Cuts at: 104 1193 3265 3311 4541 104
Size: 1089 2072 46 1230 1286
Fragments arranged by size:
2072 1286 1230 1089 46
BsiHKAI G_wGCw'C
Cuts at: 295 750 1823 2398 2737 3515 3705 4565 295
Size: 455 1073 575 339 778 190 860 1453
Fragments arranged by size:
1453 1073 860 778 575 455 339 190
Bsp1286I G_dGCh'C
Cuts at: 295 576 750 1324 1823 2398 2737 3348 3441
Size: 281 174 574 499 575 339 611 93
Cuts at: 3441 3515 3705 3767 4565 295
Size: 74 190 62 798 1453
Fragments arranged by size:
1453 798 611 575 574 499 339 281
190 174 93 74 62
BspHI T'CATG_A
Cuts at: 1703 4155 1703
Size: 2452 3271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 885 1130 1733 2439 2518 2626 2773 2987
Size: 403 245 603 706 79 108 147 214
Cuts at: 2987 3289 3670 482
Size: 302 381 2535
Fragments arranged by size:
2535 706 603 403 381 302 245 214
147 108 79
BsrBI CCG'CTC
Cuts at: 50 1095 4015 4069 5553 5718 50
Size: 1045 2920 54 1484 165 55
Fragments arranged by size:
2920 1484 1045 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3635 3635
Size: 5723
BsrFI r'CCGG_y
Cuts at: 638 727 755 939 1733 2405 2503 3721 3902
Size: 89 28 184 794 672 98 1218 181
Cuts at: 3902 638
Size: 2459
Fragments arranged by size:
2459 1218 794 672 184 181 98 89
28
BssSI C'ACGA_G
Cuts at: 1813 2161 3994 4702 1813
Size: 348 1833 708 2834
Fragments arranged by size:
2834 1833 708 348
BstAPI GCAn_nnn'nTGC
Cuts at: 662 3100 662
Size: 2438 3285
BstBI TT'CG_AA
Cuts at: 1682 4084 1682
Size: 2402 3321
BstYI r'GATC_y
Cuts at: 1 676 1128 1407 1527 2432 2480 2634 2748
Size: 675 452 279 120 905 48 154 114
Cuts at: 2748 3215 3238 3573 3819 4125 4137 4223 5275
Size: 467 23 335 246 306 12 86 1052
Cuts at: 5275 1
Size: 449
Fragments arranged by size:
1052 905 675 467 452 449 335 306
279 246 154 120 114 86 48 23
12
BtgI C'CryG_G
Cuts at: 107 1193 1946 1959 107
Size: 1086 753 13 3871
Fragments arranged by size:
3871 1086 753 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1767 1926 3673 3854 4017 1767
Size: 159 1747 181 163 3473
Fragments arranged by size:
3473 1747 181 163 159
BtsI GCAGTG_nn'
Cuts at: 576 1225 2966 576
Size: 649 1741 3333
Fragments arranged by size:
3333 1741 649
DraI TTT'AAA
Cuts at: 356 2722 356
Size: 2366 3357
DraIII CAC_nnn'GTG
Cuts at: 1818 1818
Size: 5723
DrdI GACnn_nn'nnGTC
Cuts at: 3429 4773 3429
Size: 1344 4379
EaeI y'GGCC_r
Cuts at: 797 3308 3482 3873 3900 5245 797
Size: 2511 174 391 27 1345 1275
Fragments arranged by size:
2511 1345 1275 391 174 27
EagI C'GGCC_G
Cuts at: 3308 3308
Size: 5723
EarI CTCTTCn'nnn_
Cuts at: 2207 2255 2386 2470 3746 3956 2207
Size: 48 131 84 1276 210 3974
Fragments arranged by size:
3974 1276 210 131 84 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 524 3931 4673 4819 5533 524
Size: 3407 742 146 714 714
Fragments arranged by size:
3407 742 714 714 146
EcoICRI GAG'CTC
Cuts at: 293 2735 293
Size: 2442 3281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 594 614 689 965 1091 1228 1248 2059
Size: 210 20 75 276 126 137 20 811
Cuts at: 2059 2131 2188 2329 2362 3548 3980 4333 4995
Size: 72 57 141 33 1186 432 353 662
Cuts at: 4995 384
Size: 1112
Fragments arranged by size:
1186 1112 811 662 432 353 276 210
141 137 126 75 72 57 33 20
20
EcoO109I rG'GnC_Cy
Cuts at: 999 1321 1764 2862 999
Size: 322 443 1098 3860
Fragments arranged by size:
3860 1098 443 322
EcoRI G'AATT_C
Cuts at: 2727 2727
Size: 5723
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3742 3742
Size: 5723
FspI TGC'GCA
Cuts at: 1207 3504 1207
Size: 2297 3426
HaeII r_GCGC'y
Cuts at: 1954 3157 3405 4635 5406 1954
Size: 1203 248 1230 771 2271
Fragments arranged by size:
2271 1230 1203 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 888 920 2026 2058 3565 3595 3597 3627 888
Size: 32 1106 32 1507 30 2 30 2984
Fragments arranged by size:
2984 1507 1106 32 32 30 30 2
HincII GTy'rAC
Cuts at: 211 411 1065 2762 211
Size: 200 654 1697 3172
Fragments arranged by size:
3172 1697 654 200
KasI G'GCGC_C
Cuts at: 3153 3401 3153
Size: 248 5475
KpnI G_GTAC'C
Cuts at: 2510 2743 2510
Size: 233 5490
MluI A'CGCG_T
Cuts at: 5156 5156
Size: 5723
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 443 1213 1719 4482 4666 443
Size: 770 506 2763 184 1500
Fragments arranged by size:
2763 1500 770 506 184
MscI TGG'CCA
Cuts at: 799 3484 799
Size: 2685 3038
MslI CAynn'nnrTG
Cuts at: 698 1631 1708 1747 1945 3712 3839 698
Size: 933 77 39 198 1767 127 2582
Fragments arranged by size:
2582 1767 933 198 127 77 39
MspA1I CmG'CkG
Cuts at: 462 594 988 1195 1233 2307 2664 2834 3148
Size: 132 394 207 38 1074 357 170 314
Cuts at: 3148 3508 4292 4537 462
Size: 360 784 245 1648
Fragments arranged by size:
1648 1074 784 394 360 357 314 245
207 170 132 38
NaeI GCC'GGC
Cuts at: 757 1735 3904 757
Size: 978 2169 2576
Fragments arranged by size:
2576 2169 978
NarI GG'CG_CC
Cuts at: 3154 3402 3154
Size: 248 5475
NcoI C'CATG_G
Cuts at: 107 1946 1959 107
Size: 1839 13 3871
Fragments arranged by size:
3871 1839 13
NgoMIV G'CCGG_C
Cuts at: 755 1733 3902 755
Size: 978 2169 2576
Fragments arranged by size:
2576 2169 978
NspI r_CATG'y
Cuts at: 459 2364 2776 3276 4879 459
Size: 1905 412 500 1603 1303
Fragments arranged by size:
1905 1603 1303 500 412
PasI CC'CwG_GG
Cuts at: 1317 1419 1317
Size: 102 5621
PciI A'CATG_T
Cuts at: 4875 4875
Size: 5723
PflMI CCAn_nnn'nTGG
Cuts at: 1088 1178 1793 2075 2133 1088
Size: 90 615 282 58 4678
Fragments arranged by size:
4678 615 282 90 58
PfoI T'CCnGG_A
Cuts at: 527 679 1430 2126 2279 527
Size: 152 751 696 153 3971
Fragments arranged by size:
3971 751 696 153 152
PmeI GTTT'AAAC
Cuts at: 2722 2722
Size: 5723
PmlI CAC'GTG
Cuts at: 1650 1650
Size: 5723
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 5691
PspOMI G'GGCC_C
Cuts at: 1320 1320
Size: 5723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1594 1626 1594
Size: 32 5691
PstI C_TGCA'G
Cuts at: 479 1877 2495 2770 3455 479
Size: 1398 618 275 685 2747
Fragments arranged by size:
2747 1398 685 618 275
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5723
PvuII CAG'CTG
Cuts at: 462 594 2664 3148 3508 462
Size: 132 2070 484 360 2677
Fragments arranged by size:
2677 2070 484 360 132
RsrII CG'GwC_CG
Cuts at: 3918 3918
Size: 5723
SacI G_AGCT'C
Cuts at: 295 2737 295
Size: 2442 3281
SacII CC_GC'GG
Cuts at: 1196 1196
Size: 5723
SalI G'TCGA_C
Cuts at: 2760 2760
Size: 5723
SapI GCTCTTCn'nnn_
Cuts at: 2255 2386 3746 3956 2255
Size: 131 1360 210 4022
Fragments arranged by size:
4022 1360 210 131
SbfI CC_TGCA'GG
Cuts at: 2770 2770
Size: 5723
ScaI AGT'ACT
Cuts at: 5240 5240
Size: 5723
SfcI C'TryA_G
Cuts at: 33 475 1873 2491 2766 3451 4419 4610 33
Size: 442 1398 618 275 685 968 191 1146
Fragments arranged by size:
1398 1146 968 685 618 442 275 191
SfoI GGC'GCC
Cuts at: 3155 3403 3155
Size: 248 5475
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5723
SmaI CCC'GGG
Cuts at: 1538 2745 1538
Size: 1207 4516
SmlI C'TyrA_G
Cuts at: 378 888 2878 3793 4507 4769 378
Size: 510 1990 915 714 262 1332
Fragments arranged by size:
1990 1332 915 714 510 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5723
SphI G_CATG'C
Cuts at: 2364 2776 3276 2364
Size: 412 500 4811
Fragments arranged by size:
4811 500 412
StyI C'CwwG_G
Cuts at: 107 194 1946 1959 2857 3211 107
Size: 87 1752 13 898 354 2619
Fragments arranged by size:
2619 1752 898 354 87 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2713 3306 3439 3898 4107 2713
Size: 593 133 459 209 4329
Fragments arranged by size:
4329 593 459 209 133
TatI w'GTAC_w
Cuts at: 2117 2216 5238 2117
Size: 99 3022 2602
Fragments arranged by size:
3022 2602 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 965 997 5240 5272 965
Size: 32 4243 32 1416
Fragments arranged by size:
4243 1416 32 32
Tth111I GACn'n_nGTC
Cuts at: 3520 3520
Size: 5723
XbaI T'CTAG_A
Cuts at: 2754 2754
Size: 5723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2277 3218 2277
Size: 941 4782
XmaI C'CCGG_G
Cuts at: 1536 2743 1536
Size: 1207 4516
ZraI GAC'GTC
Cuts at: 869 869
Size: 5723
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PpuMI PshAI PsiI
PspXI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI