Restriction map for
pF1KB4741
(Circular) (Six-base) MAPSORT of: pF1KB4741.seq Check: 4224 from: 1 to: 6548
pF1KB4741 6548 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 3296 3296
Size: 6548
AccI GT'mk_AC
Cuts at: 3586 3586
Size: 6548
Acc65I G'GTAC_C
Cuts at: 3564 3564
Size: 6548
AclI AA'CG_TT
Cuts at: 1173 6388 1173
Size: 5215 1333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2130 2142 2732 2761 3107 3461 3479 4373 4805
Size: 12 590 29 346 354 18 894 432
Cuts at: 4805 5158 5820 2130
Size: 353 662 2858
Fragments arranged by size:
2858 894 662 590 432 354 353 346
29 18 12
AfeI AGC'GCT
Cuts at: 258 258
Size: 6548
AflIII A'CryG_T
Cuts at: 304 3900 5700 5981 304
Size: 3596 1800 281 871
Fragments arranged by size:
3596 1800 871 281
AleI CACnn'nnGTG
Cuts at: 2183 2183
Size: 6548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2201 3032 2201
Size: 831 5717
AlwNI CAG_nnn'CTG
Cuts at: 3853 5291 3853
Size: 1438 5110
ApaLI G'TGCA_C
Cuts at: 2176 5386 2176
Size: 3210 3338
ApoI r'AATT_y
Cuts at: 966 1591 2385 2464 2544 3078 3364 3552 5901
Size: 625 794 79 80 534 286 188 2349
Cuts at: 5901 6157 966
Size: 256 1357
Fragments arranged by size:
2349 1357 794 625 534 286 256 188
80 79
AseI AT'TA_AT
Cuts at: 20 508 3034 20
Size: 488 2526 3534
Fragments arranged by size:
3534 2526 488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6548
AvaI C'yCGr_G
Cuts at: 1943 3568 1943
Size: 1625 4923
BamHI G'GATC_C
Cuts at: 2103 2430 3573 2103
Size: 327 1143 5078
Fragments arranged by size:
5078 1143 327
BanI G'GyrC_C
Cuts at: 2094 3421 3564 3978 4226 4261 2094
Size: 1327 143 414 248 35 4381
Fragments arranged by size:
4381 1327 414 248 143 35
BanII G_rGCy'C
Cuts at: 247 3562 4592 247
Size: 3315 1030 2203
Fragments arranged by size:
3315 2203 1030
BbeI G_GCGC'C
Cuts at: 3982 4230 3982
Size: 248 6300
BbsI GAAGACnn'nnnn_
Cuts at: 1581 1581
Size: 6548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2282 2316 2282
Size: 34 6514
BciVI GTATCCnnnnn_n'
Cuts at: 4446 5502 6548 4446
Size: 1056 1046 4446
Fragments arranged by size:
4446 1056 1046
BclI T'GATC_A
Cuts at: 947 4068 947
Size: 3121 3427
BfrBI ATG'CAT
Cuts at: 1128 2683 1128
Size: 1555 4993
BglII A'GATC_T
Cuts at: 1251 4063 6100 1251
Size: 2812 2037 1699
Fragments arranged by size:
2812 2037 1699
BlpI GC'TnA_GC
Cuts at: 3660 3660
Size: 6548
Bme1580I G_kGCm'C
Cuts at: 1942 2180 3426 4173 4266 5390 1942
Size: 238 1246 747 93 1124 3100
Fragments arranged by size:
3100 1246 1124 747 238 93
BmrI ACTGGGnnnn_n'
Cuts at: 2319 2432 2859 3760 3841 4176 6320 2319
Size: 113 427 901 81 335 2144 2547
Fragments arranged by size:
2547 2144 901 427 335 113 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 4915 3131
Size: 1784 4764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1854 2514 3018 1331
Size: 523 660 504 4861
Fragments arranged by size:
4861 660 523 504
Bpu10I CC'TnA_GC
Cuts at: 2287 2729 2287
Size: 442 6106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 4603 5317 5615 3724
Size: 879 714 298 4657
Fragments arranged by size:
4657 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2740 2819 6252 2740
Size: 79 3433 3036
Fragments arranged by size:
3433 3036 79
BsaAI yAC'GTr
Cuts at: 3901 3901
Size: 6548
BsaBI GATnn'nnATC
Cuts at: 6 4062 6099 6
Size: 4056 2037 455
Fragments arranged by size:
4056 2037 455
BsaHI Gr'CG_yC
Cuts at: 3293 3979 4227 4929 6425 3293
Size: 686 248 702 1496 3416
Fragments arranged by size:
3416 1496 702 686 248
BsaWI w'CCGG_w
Cuts at: 3737 4258 4936 5347 5494 5768 3737
Size: 521 678 411 147 274 4517
Fragments arranged by size:
4517 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1946 1976 1946
Size: 30 6518
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1064 2107 1064
Size: 1043 5505
BseYI C'CCAG_C
Cuts at: 3973 4731 5396 5804 3973
Size: 758 665 408 4717
Fragments arranged by size:
4717 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 821 1781 1931 821
Size: 960 150 5438
Fragments arranged by size:
5438 960 150
BsiEI CG_ry'CG
Cuts at: 104 4090 4136 5366 104
Size: 3986 46 1230 1286
Fragments arranged by size:
3986 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1074 2180 2844 3562 4340 4530 5390 1074
Size: 1106 664 718 778 190 860 2232
Fragments arranged by size:
2232 1106 860 778 718 664 190
BsmI GAATG_Cn'
Cuts at: 2145 2145
Size: 6548
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 2453
Size: 6548
Bsp1286I G_dGCh'C
Cuts at: 247 1074 1942 2180 2844 3426 3562 4173 4266
Size: 827 868 238 664 582 136 611 93
Cuts at: 4266 4340 4530 4592 5390 247
Size: 74 190 62 798 1405
Fragments arranged by size:
1405 868 827 798 664 611 582 238
190 136 93 74 62
BspHI T'CATG_A
Cuts at: 4980 4980
Size: 6548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3598 3812 4114 4495 3598
Size: 214 302 381 5651
Fragments arranged by size:
5651 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4840 4894 6378 6543 50
Size: 4790 54 1484 165 55
Fragments arranged by size:
4790 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1013 1052 1790 3296 4460 1013
Size: 39 738 1506 1164 3101
Fragments arranged by size:
3101 1506 1164 738 39
BsrFI r'CCGG_y
Cuts at: 4546 4727 4546
Size: 181 6367
BsrGI T'GTAC_A
Cuts at: 556 556
Size: 6548
BssSI C'ACGA_G
Cuts at: 3474 4819 5527 3474
Size: 1345 708 4495
Fragments arranged by size:
4495 1345 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3925 3925
Size: 6548
BstBI TT'CG_AA
Cuts at: 4909 4909
Size: 6548
BstYI r'GATC_y
Cuts at: 1 1251 1447 2103 2430 3139 3573 4040 4063
Size: 1250 196 656 327 709 434 467 23
Cuts at: 4063 4398 4644 4950 4962 5048 6100 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1250 1052 709 656 467 449 434 335
327 306 246 196 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2099 2099
Size: 6548
BtgI C'CryG_G
Cuts at: 1606 2088 1606
Size: 482 6066
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4498 4679 4842 4498
Size: 181 163 6204
Fragments arranged by size:
6204 181 163
BtsI GCAGTG_nn'
Cuts at: 2178 2190 3126 3791 2178
Size: 12 936 665 4935
Fragments arranged by size:
4935 936 665 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1848 1883 1848
Size: 35 6513
DraI TTT'AAA
Cuts at: 593 971 3530 3547 593
Size: 378 2559 17 3594
Fragments arranged by size:
3594 2559 378 17
DraIII CAC_nnn'GTG
Cuts at: 2094 2440 3421 2094
Size: 346 981 5221
Fragments arranged by size:
5221 981 346
DrdI GACnn_nn'nnGTC
Cuts at: 4254 5598 4254
Size: 1344 5204
EaeI y'GGCC_r
Cuts at: 1921 2080 4133 4307 4698 4725 6070 1921
Size: 159 2053 174 391 27 1345 2399
Fragments arranged by size:
2399 2053 1345 391 174 159 27
EagI C'GGCC_G
Cuts at: 4133 4133
Size: 6548
EarI CTCTTCn'nnn_
Cuts at: 1928 2043 3194 4571 4781 1928
Size: 115 1151 1377 210 3695
Fragments arranged by size:
3695 1377 1151 210 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4756 5498 5644 6358 4756
Size: 742 146 714 4946
Fragments arranged by size:
4946 742 714 146
EcoICRI GAG'CTC
Cuts at: 3560 3560
Size: 6548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 1854 2130 2142 2514 2732 2761 3018 3107
Size: 523 276 12 372 218 29 257 89
Cuts at: 3107 3461 3479 4373 4805 5158 5820 1331
Size: 354 18 894 432 353 662 2059
Fragments arranged by size:
2059 894 662 523 432 372 354 353
276 257 218 89 29 18 12
EcoO109I rG'GnC_Cy
Cuts at: 1804 3687 1804
Size: 1883 4665
EcoRI G'AATT_C
Cuts at: 2464 2544 3078 3552 2464
Size: 80 534 474 5460
Fragments arranged by size:
5460 534 474 80
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1594 3542 4567 1594
Size: 1948 1025 3575
Fragments arranged by size:
3575 1948 1025
FspI TGC'GCA
Cuts at: 4329 4329
Size: 6548
HaeII r_GCGC'y
Cuts at: 260 3982 4230 5460 6231 260
Size: 3722 248 1230 771 577
Fragments arranged by size:
3722 1230 771 577 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1752 2620 2652 2712 2744 4390 4420 4422
Size: 32 868 32 60 32 1646 30 2
Cuts at: 4422 4452 1720
Size: 30 3816
Fragments arranged by size:
3816 1646 868 60 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1677 2889 3587 1677
Size: 1212 698 4638
Fragments arranged by size:
4638 1212 698
KasI G'GCGC_C
Cuts at: 3978 4226 3978
Size: 248 6300
KpnI G_GTAC'C
Cuts at: 3568 3568
Size: 6548
MluI A'CGCG_T
Cuts at: 5981 5981
Size: 6548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1520 5307 5491 1520
Size: 3787 184 2577
Fragments arranged by size:
3787 2577 184
MscI TGG'CCA
Cuts at: 2082 4309 2082
Size: 2227 4321
MslI CAynn'nnrTG
Cuts at: 112 331 2173 2183 4537 4664 112
Size: 219 1842 10 2354 127 1996
Fragments arranged by size:
2354 1996 1842 219 127 10
MspA1I CmG'CkG
Cuts at: 1608 2078 3659 3973 4333 5117 5362 1608
Size: 470 1581 314 360 784 245 2794
Fragments arranged by size:
2794 1581 784 470 360 314 245
NaeI GCC'GGC
Cuts at: 4729 4729
Size: 6548
NarI GG'CG_CC
Cuts at: 3979 4227 3979
Size: 248 6300
NdeI CA'TA_TG
Cuts at: 2562 2871 2562
Size: 309 6239
NgoMIV G'CCGG_C
Cuts at: 4727 4727
Size: 6548
NsiI A_TGCA'T
Cuts at: 1130 2685 1130
Size: 1555 4993
NspI r_CATG'y
Cuts at: 308 1006 1132 3601 4101 5704 308
Size: 698 126 2469 500 1603 1152
Fragments arranged by size:
2469 1603 1152 698 500 126
PasI CC'CwG_GG
Cuts at: 1148 1808 1148
Size: 660 5888
PciI A'CATG_T
Cuts at: 304 5700 304
Size: 5396 1152
PfoI T'CCnGG_A
Cuts at: 798 1308 798
Size: 510 6038
PmeI GTTT'AAAC
Cuts at: 3547 3547
Size: 6548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4994 5026 4994
Size: 32 6516
PpuMI rG'GwC_Cy
Cuts at: 1804 1804
Size: 6548
PsiI TTA'TAA
Cuts at: 1346 1550 1697 2692 1346
Size: 204 147 995 5202
Fragments arranged by size:
5202 995 204 147
PstI C_TGCA'G
Cuts at: 2185 3021 3133 3595 4280 2185
Size: 836 112 462 685 4453
Fragments arranged by size:
4453 836 685 462 112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6548
PvuII CAG'CTG
Cuts at: 2078 3973 4333 2078
Size: 1895 360 4293
Fragments arranged by size:
4293 1895 360
RsrII CG'GwC_CG
Cuts at: 4743 4743
Size: 6548
SacI G_AGCT'C
Cuts at: 3562 3562
Size: 6548
SacII CC_GC'GG
Cuts at: 1609 1609
Size: 6548
SalI G'TCGA_C
Cuts at: 3585 3585
Size: 6548
SapI GCTCTTCn'nnn_
Cuts at: 4571 4781 4571
Size: 210 6338
SbfI CC_TGCA'GG
Cuts at: 3595 3595
Size: 6548
ScaI AGT'ACT
Cuts at: 2848 6065 2848
Size: 3217 3331
SfcI C'TryA_G
Cuts at: 33 642 2181 3017 3129 3591 4276 5244 5435
Size: 609 1539 836 112 462 685 968 191
Cuts at: 5435 33
Size: 1146
Fragments arranged by size:
1539 1146 968 836 685 609 462 191
112
SfoI GGC'GCC
Cuts at: 3980 4228 3980
Size: 248 6300
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6548
SmaI CCC'GGG
Cuts at: 3570 3570
Size: 6548
SmlI C'TyrA_G
Cuts at: 3703 4618 5332 5594 3703
Size: 915 714 262 4657
Fragments arranged by size:
4657 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6548
SphI G_CATG'C
Cuts at: 3601 4101 3601
Size: 500 6048
SspI AAT'ATT
Cuts at: 512 606 636 1261 3158 512
Size: 94 30 625 1897 3902
Fragments arranged by size:
3902 1897 625 94 30
StuI AGG'CCT
Cuts at: 1143 1285 2240 1143
Size: 142 955 5451
Fragments arranged by size:
5451 955 142
StyI C'CwwG_G
Cuts at: 3026 3682 4036 3026
Size: 656 354 5538
Fragments arranged by size:
5538 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2451 4131 4264 4723 4932 2451
Size: 1680 133 459 209 4067
Fragments arranged by size:
4067 1680 459 209 133
TatI w'GTAC_w
Cuts at: 487 556 2327 2846 3109 6063 487
Size: 69 1771 519 263 2954 972
Fragments arranged by size:
2954 1771 972 519 263 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1893 1925 2039 2071 6065 6097 1893
Size: 32 114 32 3994 32 2344
Fragments arranged by size:
3994 2344 114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4345 4345
Size: 6548
XbaI T'CTAG_A
Cuts at: 3579 3579
Size: 6548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1085 4043 1085
Size: 2958 3590
XmaI C'CCGG_G
Cuts at: 3568 3568
Size: 6548
XmnI GAAnn'nnTTC
Cuts at: 539 2143 539
Size: 1604 4944
ZraI GAC'GTC
Cuts at: 3294 3294
Size: 6548
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PasI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BsiWI BspEI BssHII
BstEII BstXI BstZ17I ClaI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI PacI
PflMI PmlI PshAI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI