Restriction map for
pF1KB4745
(Circular) (Six-base) MAPSORT of: pF1KB4745.seq Check: 7444 from: 1 to: 4232
pF1KB4745 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 947 1270 947
Size: 323 3909
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 2057 2489 2842 3504 388
Size: 1669 432 353 662 1116
Fragments arranged by size:
1669 1116 662 432 353
AfeI AGC'GCT
Cuts at: 529 529
Size: 4232
AflIII A'CryG_T
Cuts at: 1584 3384 3665 1584
Size: 1800 281 2151
Fragments arranged by size:
2151 1800 281
AleI CACnn'nnGTG
Cuts at: 805 805
Size: 4232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 271 303 271
Size: 32 4200
AlwNI CAG_nnn'CTG
Cuts at: 204 717 1537 2975 204
Size: 513 820 1438 1461
Fragments arranged by size:
1461 1438 820 513
ApaI G_GGCC'C
Cuts at: 449 449
Size: 4232
ApaLI G'TGCA_C
Cuts at: 1079 3070 1079
Size: 1991 2241
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 453 1252 453
Size: 799 3433
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 1248 1662 1910 1945 1248
Size: 414 248 35 3535
Fragments arranged by size:
3535 414 248 35
BanII G_rGCy'C
Cuts at: 141 449 659 1246 2276 141
Size: 308 210 587 1030 2097
Fragments arranged by size:
2097 1030 587 308 210
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BciVI GTATCCnnnnn_n'
Cuts at: 2130 3186 4232 2130
Size: 1056 1046 2130
Fragments arranged by size:
2130 1056 1046
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 449 1083 1857 1950 3074 449
Size: 634 774 93 1124 1607
Fragments arranged by size:
1607 1124 774 634 93
BmgBI CAC'GTC
Cuts at: 972 972
Size: 4232
BmrI ACTGGGnnnn_n'
Cuts at: 452 1444 1525 1860 4004 452
Size: 992 81 335 2144 680
Fragments arranged by size:
2144 992 680 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1235 2599 1235
Size: 1364 2868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 763 907 1045 587
Size: 176 144 138 3774
Fragments arranged by size:
3774 176 144 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 3001 3299 1408
Size: 879 714 298 2341
Fragments arranged by size:
2341 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 126 3936 126
Size: 3810 422
BsaAI yAC'GTr
Cuts at: 147 1585 147
Size: 1438 2794
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 979 1663 1911 2613 4109 979
Size: 684 248 702 1496 1102
Fragments arranged by size:
1496 1102 702 684 248
BsaWI w'CCGG_w
Cuts at: 698 1421 1942 2620 3031 3178 3452 698
Size: 723 521 678 411 147 274 1478
Fragments arranged by size:
1478 723 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 271 301 271
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 569 685 760 569
Size: 116 75 4041
Fragments arranged by size:
4041 116 75
BseYI C'CCAG_C
Cuts at: 1657 2415 3080 3488 1657
Size: 758 665 408 2401
Fragments arranged by size:
2401 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 730 805 816 127
Size: 603 75 11 3543
Fragments arranged by size:
3543 603 75 11
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3050 104
Size: 1670 46 1230 1286
Fragments arranged by size:
1670 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 141 659 903 920 1083 1246 2024 2214 3074
Size: 518 244 17 163 163 778 190 860
Cuts at: 3074 141
Size: 1299
Fragments arranged by size:
1299 860 778 518 244 190 163 163
17
BsmBI CGTCTCn'nnnn_
Cuts at: 1194 1194
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 141 449 659 903 920 1083 1246 1857 1950
Size: 308 210 244 17 163 163 611 93
Cuts at: 1950 2024 2214 2276 3074 141
Size: 74 190 62 798 1299
Fragments arranged by size:
1299 798 611 308 244 210 190 163
163 93 74 62 17
BspEI T'CCGG_A
Cuts at: 698 698
Size: 4232
BspHI T'CATG_A
Cuts at: 2664 2664
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 1282 1496 1798 2179 510
Size: 772 214 302 381 2563
Fragments arranged by size:
2563 772 381 302 214
BsrBI CCG'CTC
Cuts at: 50 729 2524 2578 4062 4227 50
Size: 679 1795 54 1484 165 55
Fragments arranged by size:
1795 1484 679 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 835 1000 1148 2230 2411 835
Size: 165 148 1082 181 2656
Fragments arranged by size:
2656 1082 181 165 148
BssSI C'ACGA_G
Cuts at: 2503 3211 2503
Size: 708 3524
BstAPI GCAn_nnn'nTGC
Cuts at: 717 1609 717
Size: 892 3340
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 819 1132 819
Size: 313 3919
BstYI r'GATC_y
Cuts at: 1 689 1257 1724 1747 2082 2328 2634 2646
Size: 688 568 467 23 335 246 306 12
Cuts at: 2646 2732 3784 1
Size: 86 1052 449
Fragments arranged by size:
1052 688 568 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 275 431 1022 1100 107
Size: 168 156 591 78 3239
Fragments arranged by size:
3239 591 168 156 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1002 2182 2363 2526 1002
Size: 1180 181 163 2708
Fragments arranged by size:
2708 1180 181 163
BtsI GCAGTG_nn'
Cuts at: 900 1475 900
Size: 575 3657
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DraIII CAC_nnn'GTG
Cuts at: 150 255 150
Size: 105 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1186 1938 3282 1186
Size: 752 1344 2136
Fragments arranged by size:
2136 1344 752
EaeI y'GGCC_r
Cuts at: 428 809 1025 1817 1991 2382 2409 3754 428
Size: 381 216 792 174 391 27 1345 906
Fragments arranged by size:
1345 906 792 391 381 216 174 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 846 2440 3182 3328 4042 846
Size: 1594 742 146 714 1036
Fragments arranged by size:
1594 1036 742 714 146
EcoICRI GAG'CTC
Cuts at: 139 657 1244 139
Size: 518 587 3127
Fragments arranged by size:
3127 587 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 587 763 907 1045 2057 2489 2842 3504
Size: 199 176 144 138 1012 432 353 662
Cuts at: 3504 388
Size: 1116
Fragments arranged by size:
1116 1012 662 432 353 199 176 144
138
EcoNI CCTnn'n_nnAGG
Cuts at: 700 700
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 113 201 402 446 1179 1371 113
Size: 88 201 44 733 192 2974
Fragments arranged by size:
2974 733 201 192 88 44
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 258 351 2251 258
Size: 93 1900 2239
Fragments arranged by size:
2239 1900 93
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 531 1666 1914 3144 3915 531
Size: 1135 248 1230 771 848
Fragments arranged by size:
1230 1135 848 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 761 793 978 1010 2074 2104 2106 2136 761
Size: 32 185 32 1064 30 2 30 2857
Fragments arranged by size:
2857 1064 185 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1038 1271 1038
Size: 233 3999
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2991 3175 2991
Size: 184 4048
MscI TGG'CCA
Cuts at: 430 811 1027 1993 430
Size: 381 216 966 2669
Fragments arranged by size:
2669 966 381 216
MslI CAynn'nnrTG
Cuts at: 223 251 544 781 805 1021 2221 2348 223
Size: 28 293 237 24 216 1200 127 2107
Fragments arranged by size:
2107 1200 293 237 216 127 28 24
MspA1I CmG'CkG
Cuts at: 195 714 1053 1343 1657 2017 2801 3046 195
Size: 519 339 290 314 360 784 245 1381
Fragments arranged by size:
1381 784 519 360 339 314 290 245
NaeI GCC'GGC
Cuts at: 1002 1150 2413 1002
Size: 148 1263 2821
Fragments arranged by size:
2821 1263 148
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NcoI C'CATG_G
Cuts at: 107 1100 107
Size: 993 3239
NgoMIV G'CCGG_C
Cuts at: 1000 1148 2411 1000
Size: 148 1263 2821
Fragments arranged by size:
2821 1263 148
NspI r_CATG'y
Cuts at: 1285 1785 3388 1285
Size: 500 1603 2129
Fragments arranged by size:
2129 1603 500
PciI A'CATG_T
Cuts at: 3384 3384
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 255 255
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PmlI CAC'GTG
Cuts at: 147 147
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 271 303 2678 2710 271
Size: 32 2375 32 1793
Fragments arranged by size:
2375 1793 32 32
PpuMI rG'GwC_Cy
Cuts at: 113 1179 113
Size: 1066 3166
PspOMI G'GGCC_C
Cuts at: 445 445
Size: 4232
PstI C_TGCA'G
Cuts at: 200 392 1279 1964 200
Size: 192 887 685 2468
Fragments arranged by size:
2468 887 685 192
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 195 714 1657 2017 195
Size: 519 943 360 2410
Fragments arranged by size:
2410 943 519 360
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 141 659 1246 141
Size: 518 587 3127
Fragments arranged by size:
3127 587 518
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SanDI GG'GwC_CC
Cuts at: 113 113
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SexAI A'CCwGG_T
Cuts at: 818 818
Size: 4232
SfcI C'TryA_G
Cuts at: 33 196 388 1275 1960 2928 3119 33
Size: 163 192 887 685 968 191 1146
Fragments arranged by size:
1146 968 887 685 192 191 163
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 455 1254 455
Size: 799 3433
SmlI C'TyrA_G
Cuts at: 1387 2302 3016 3278 1387
Size: 915 714 262 2341
Fragments arranged by size:
2341 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
StuI AGG'CCT
Cuts at: 499 1066 499
Size: 567 3665
StyI C'CwwG_G
Cuts at: 107 1100 1366 1720 107
Size: 993 266 354 2619
Fragments arranged by size:
2619 993 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 1815 1948 2407 2616 148
Size: 1667 133 459 209 1764
Fragments arranged by size:
1764 1667 459 209 133
TatI w'GTAC_w
Cuts at: 773 3747 773
Size: 2974 1258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1073 1105 3749 3781 1073
Size: 32 2644 32 1524
Fragments arranged by size:
2644 1524 32 32
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1020 1727 1020
Size: 707 3525
XmaI C'CCGG_G
Cuts at: 453 1252 453
Size: 799 3433
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI