Restriction map for
pF1KB4753
(Circular) (Six-base) MAPSORT of: pF1KB4753.seq Check: 1990 from: 1 to: 3437
pF1KB4753 3437 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 475 475
Size: 3437
Acc65I G'GTAC_C
Cuts at: 453 453
Size: 3437
AclI AA'CG_TT
Cuts at: 3277 3277
Size: 3437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1262 1694 2441 2709 337
Size: 925 432 747 268 1065
Fragments arranged by size:
1065 925 747 432 268
AflIII A'CryG_T
Cuts at: 185 789 1893 2870 185
Size: 604 1104 977 752
Fragments arranged by size:
1104 977 752 604
AgeI A'CCGG_T
Cuts at: 1825 1825
Size: 3437
AlwNI CAG_nnn'CTG
Cuts at: 742 2309 742
Size: 1567 1870
ApaLI G'TGCA_C
Cuts at: 199 2207 199
Size: 2008 1429
ApoI r'AATT_y
Cuts at: 441 2790 3046 441
Size: 2349 256 832
Fragments arranged by size:
2349 832 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3437
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3437
AvaI C'yCGr_G
Cuts at: 457 457
Size: 3437
BamHI G'GATC_C
Cuts at: 462 462
Size: 3437
BanI G'GyrC_C
Cuts at: 453 867 1115 1150 453
Size: 414 248 35 2740
Fragments arranged by size:
2740 414 248 35
BanII G_rGCy'C
Cuts at: 451 1481 451
Size: 1030 2407
BbeI G_GCGC'C
Cuts at: 871 1119 871
Size: 248 3189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3403
BciVI GTATCCnnnnn_n'
Cuts at: 1335 2096 3437 1335
Size: 761 1341 1335
Fragments arranged by size:
1341 1335 761
BclI T'GATC_A
Cuts at: 957 957
Size: 3437
BglII A'GATC_T
Cuts at: 952 2989 952
Size: 2037 1400
BlpI GC'TnA_GC
Cuts at: 549 549
Size: 3437
Bme1580I G_kGCm'C
Cuts at: 193 203 1062 1155 2211 193
Size: 10 859 93 1056 1419
Fragments arranged by size:
1419 1056 859 93 10
BmrI ACTGGGnnnn_n'
Cuts at: 649 730 1065 3209 649
Size: 81 335 2144 877
Fragments arranged by size:
2144 877 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 444
Size: 3437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 1492 1984 2282 613
Size: 879 492 298 1768
Fragments arranged by size:
1768 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3141 3141
Size: 3437
BsaAI yAC'GTr
Cuts at: 188 198 790 188
Size: 10 592 2835
Fragments arranged by size:
2835 592 10
BsaBI GATnn'nnATC
Cuts at: 6 951 2988 6
Size: 945 2037 455
Fragments arranged by size:
2037 945 455
BsaHI Gr'CG_yC
Cuts at: 868 1116 1818 3314 868
Size: 248 702 1496 991
Fragments arranged by size:
1496 991 702 248
BsaWI w'CCGG_w
Cuts at: 626 1147 1825 2099 2246 2657 626
Size: 521 678 274 147 411 1406
Fragments arranged by size:
1406 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 166
Size: 3437
BseYI C'CCAG_C
Cuts at: 862 1620 2197 2693 862
Size: 758 577 496 1606
Fragments arranged by size:
1606 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 979 1025 2233 104
Size: 875 46 1208 1308
Fragments arranged by size:
1308 1208 875 46
BsiHKAI G_wGCw'C
Cuts at: 203 451 1229 1419 2211 203
Size: 248 778 190 792 1429
Fragments arranged by size:
1429 792 778 248 190
Bsp1286I G_dGCh'C
Cuts at: 193 203 451 1062 1155 1229 1419 1481 2211
Size: 10 248 611 93 74 190 62 730
Cuts at: 2211 193
Size: 1419
Fragments arranged by size:
1419 730 611 248 190 93 74 62
10
BspEI T'CCGG_A
Cuts at: 2657 2657
Size: 3437
BspHI T'CATG_A
Cuts at: 2613 2613
Size: 3437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 487 701 1003 1384 487
Size: 214 302 381 2540
Fragments arranged by size:
2540 381 302 214
BsrBI CCG'CTC
Cuts at: 50 362 1729 1783 3267 3432 50
Size: 312 1367 54 1484 165 55
Fragments arranged by size:
1484 1367 312 165 55 54
BsrDI GCAATG_nn'
Cuts at: 408 1349 408
Size: 941 2496
BsrFI r'CCGG_y
Cuts at: 1435 1616 1825 1435
Size: 181 209 3047
Fragments arranged by size:
3047 209 181
BssSI C'ACGA_G
Cuts at: 332 1708 2066 332
Size: 1376 358 1703
Fragments arranged by size:
1703 1376 358
BstAPI GCAn_nnn'nTGC
Cuts at: 188 814 188
Size: 626 2811
BstBI TT'CG_AA
Cuts at: 1798 1798
Size: 3437
BstYI r'GATC_y
Cuts at: 1 462 929 952 1287 1533 2545 2631 2643
Size: 461 467 23 335 246 1012 86 12
Cuts at: 2643 2989 1
Size: 346 449
Fragments arranged by size:
1012 467 461 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1387 1568 1731 1387
Size: 181 163 3093
Fragments arranged by size:
3093 181 163
BtsI GCAGTG_nn'
Cuts at: 680 680
Size: 3437
DraI TTT'AAA
Cuts at: 436 436
Size: 3437
DrdI GACnn_nn'nnGTC
Cuts at: 1143 2001 1143
Size: 858 2579
EaeI y'GGCC_r
Cuts at: 1022 1196 1587 1614 2959 1022
Size: 174 391 27 1345 1500
Fragments arranged by size:
1500 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1022 1022
Size: 3437
EarI CTCTTCn'nnn_
Cuts at: 1460 1670 1460
Size: 210 3227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1645 1955 2101 3247 1645
Size: 310 146 1146 1835
Fragments arranged by size:
1835 1146 310 146
EcoICRI GAG'CTC
Cuts at: 449 449
Size: 3437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 444 1262 1694 2441 2709 337
Size: 107 818 432 747 268 1065
Fragments arranged by size:
1065 818 747 432 268 107
EcoO109I rG'GnC_Cy
Cuts at: 576 576
Size: 3437
EcoRI G'AATT_C
Cuts at: 441 441
Size: 3437
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1456 1456
Size: 3437
FspI TGC'GCA
Cuts at: 1218 1218
Size: 3437
HaeII r_GCGC'y
Cuts at: 871 1119 2141 3120 871
Size: 248 1022 979 1188
Fragments arranged by size:
1188 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1279 1309 1311 1341 1279
Size: 30 2 30 3375
Fragments arranged by size:
3375 30 30 2
HincII GTy'rAC
Cuts at: 294 476 294
Size: 182 3255
KasI G'GCGC_C
Cuts at: 867 1115 867
Size: 248 3189
KpnI G_GTAC'C
Cuts at: 457 457
Size: 3437
MluI A'CGCG_T
Cuts at: 2870 2870
Size: 3437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2292 2108
Size: 184 3253
MscI TGG'CCA
Cuts at: 1198 1198
Size: 3437
MslI CAynn'nnrTG
Cuts at: 332 1426 1553 332
Size: 1094 127 2216
Fragments arranged by size:
2216 1094 127
MspA1I CmG'CkG
Cuts at: 277 422 548 862 1222 2235 2480 277
Size: 145 126 314 360 1013 245 1234
Fragments arranged by size:
1234 1013 360 314 245 145 126
NaeI GCC'GGC
Cuts at: 1618 1618
Size: 3437
NarI GG'CG_CC
Cuts at: 868 1116 868
Size: 248 3189
NgoMIV G'CCGG_C
Cuts at: 1616 1616
Size: 3437
NspI r_CATG'y
Cuts at: 490 990 1897 490
Size: 500 907 2030
Fragments arranged by size:
2030 907 500
PciI A'CATG_T
Cuts at: 1893 1893
Size: 3437
PmeI GTTT'AAAC
Cuts at: 436 436
Size: 3437
PmlI CAC'GTG
Cuts at: 188 188
Size: 3437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2576 2608 2576
Size: 32 3405
PstI C_TGCA'G
Cuts at: 306 369 484 1169 306
Size: 63 115 685 2574
Fragments arranged by size:
2574 685 115 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3437
PvuII CAG'CTG
Cuts at: 277 862 1222 277
Size: 585 360 2492
Fragments arranged by size:
2492 585 360
RsrII CG'GwC_CG
Cuts at: 1632 1632
Size: 3437
SacI G_AGCT'C
Cuts at: 451 451
Size: 3437
SalI G'TCGA_C
Cuts at: 474 474
Size: 3437
SapI GCTCTTCn'nnn_
Cuts at: 1460 1670 1460
Size: 210 3227
SbfI CC_TGCA'GG
Cuts at: 484 484
Size: 3437
ScaI AGT'ACT
Cuts at: 2954 2954
Size: 3437
SfcI C'TryA_G
Cuts at: 33 302 365 480 1165 2158 2349 33
Size: 269 63 115 685 993 191 1121
Fragments arranged by size:
1121 993 685 269 191 115 63
SfoI GGC'GCC
Cuts at: 869 1117 869
Size: 248 3189
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3437
SmaI CCC'GGG
Cuts at: 459 459
Size: 3437
SmlI C'TyrA_G
Cuts at: 592 1507 1999 2261 592
Size: 915 492 262 1768
Fragments arranged by size:
1768 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3437
SphI G_CATG'C
Cuts at: 490 990 490
Size: 500 2937
StyI C'CwwG_G
Cuts at: 571 925 571
Size: 354 3083
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1020 1153 1612 1821 1020
Size: 133 459 209 2636
Fragments arranged by size:
2636 459 209 133
TatI w'GTAC_w
Cuts at: 2952 2952
Size: 3437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 3405
Tth111I GACn'n_nGTC
Cuts at: 1234 1234
Size: 3437
XbaI T'CTAG_A
Cuts at: 468 468
Size: 3437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 932 932
Size: 3437
XmaI C'CCGG_G
Cuts at: 457 457
Size: 3437
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI