Restriction map for
pF1KB4757
(Circular) (Six-base) MAPSORT of: pF1KB4757.seq Check: 3628 from: 1 to: 4745
pF1KB4757 4745 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1459 1459
Size: 4745
AccI GT'mk_AC
Cuts at: 1783 1783
Size: 4745
Acc65I G'GTAC_C
Cuts at: 1761 1761
Size: 4745
AclI AA'CG_TT
Cuts at: 4585 4585
Size: 4745
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 1674 2570 3002 3355 4017 149
Size: 1525 896 432 353 662 877
Fragments arranged by size:
1525 896 877 662 432 353
AflIII A'CryG_T
Cuts at: 454 658 2097 3897 4178 454
Size: 204 1439 1800 281 1021
Fragments arranged by size:
1800 1439 1021 281 204
AleI CACnn'nnGTG
Cuts at: 727 977 1565 1625 1649 1733 727
Size: 250 588 60 24 84 3739
Fragments arranged by size:
3739 588 250 84 60 24
AlwNI CAG_nnn'CTG
Cuts at: 2050 3488 2050
Size: 1438 3307
ApaLI G'TGCA_C
Cuts at: 3583 3583
Size: 4745
ApoI r'AATT_y
Cuts at: 716 800 843 884 968 1052 1136 1220 1472
Size: 84 43 41 84 84 84 84 252
Cuts at: 1472 1640 1749 4098 4354 716
Size: 168 109 2349 256 1107
Fragments arranged by size:
2349 1107 256 252 168 109 84 84
84 84 84 43 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4745
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
AvaI C'yCGr_G
Cuts at: 1765 1765
Size: 4745
AvrII C'CTAG_G
Cuts at: 313 313
Size: 4745
BamHI G'GATC_C
Cuts at: 1770 1770
Size: 4745
BanI G'GyrC_C
Cuts at: 1379 1761 2175 2423 2458 1379
Size: 382 414 248 35 3666
Fragments arranged by size:
3666 414 382 248 35
BanII G_rGCy'C
Cuts at: 610 1198 1759 2789 610
Size: 588 561 1030 2566
Fragments arranged by size:
2566 1030 588 561
BbeI G_GCGC'C
Cuts at: 2179 2427 2179
Size: 248 4497
BciVI GTATCCnnnnn_n'
Cuts at: 2643 3699 4745 2643
Size: 1056 1046 2643
Fragments arranged by size:
2643 1056 1046
BclI T'GATC_A
Cuts at: 2265 2265
Size: 4745
BfrBI ATG'CAT
Cuts at: 286 286
Size: 4745
BglII A'GATC_T
Cuts at: 2260 4297 2260
Size: 2037 2708
BlpI GC'TnA_GC
Cuts at: 1857 1857
Size: 4745
Bme1580I G_kGCm'C
Cuts at: 2370 2463 3587 2370
Size: 93 1124 3528
Fragments arranged by size:
3528 1124 93
BmgBI CAC'GTC
Cuts at: 457 457
Size: 4745
BmrI ACTGGGnnnn_n'
Cuts at: 1070 1957 2038 2373 4517 1070
Size: 887 81 335 2144 1298
Fragments arranged by size:
2144 1298 887 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1217 3112 1217
Size: 1895 2850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 662 746 830 914 1250 1418 1502 211
Size: 451 84 84 84 336 168 84 3454
Fragments arranged by size:
3454 451 336 168 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 1921 2800 3514 3812 426
Size: 1495 879 714 298 1359
Fragments arranged by size:
1495 1359 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 642 1314 4449 642
Size: 672 3135 938
Fragments arranged by size:
3135 938 672
BsaAI yAC'GTr
Cuts at: 2098 2098
Size: 4745
BsaBI GATnn'nnATC
Cuts at: 6 774 2259 4296 6
Size: 768 1485 2037 455
Fragments arranged by size:
2037 1485 768 455
BsaHI Gr'CG_yC
Cuts at: 2176 2424 3126 4622 2176
Size: 248 702 1496 2299
Fragments arranged by size:
2299 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1934 2455 3133 3544 3691 3965 1934
Size: 521 678 411 147 274 2714
Fragments arranged by size:
2714 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 164 2170 2928 3593 4001 164
Size: 2006 758 665 408 908
Fragments arranged by size:
2006 908 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 537
Size: 4745
BsiEI CG_ry'CG
Cuts at: 104 2287 2333 3563 104
Size: 2183 46 1230 1286
Fragments arranged by size:
2183 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 610 1282 1759 2537 2727 3587 610
Size: 672 477 778 190 860 1768
Fragments arranged by size:
1768 860 778 672 477 190
BsmI GAATG_Cn'
Cuts at: 931 931
Size: 4745
BsmBI CGTCTCn'nnnn_
Cuts at: 463 463
Size: 4745
Bsp1286I G_dGCh'C
Cuts at: 610 1198 1282 1759 2370 2463 2537 2727 2789
Size: 588 84 477 611 93 74 190 62
Cuts at: 2789 3587 610
Size: 798 1768
Fragments arranged by size:
1768 798 611 588 477 190 93 84
74 62
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1459 1795 2009 2311 2692 1459
Size: 336 214 302 381 3512
Fragments arranged by size:
3512 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 3037 3091 4575 4740 50
Size: 2987 54 1484 165 55
Fragments arranged by size:
2987 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 837 1425 2657 837
Size: 588 1232 2925
Fragments arranged by size:
2925 1232 588
BsrFI r'CCGG_y
Cuts at: 2743 2924 2743
Size: 181 4564
BssSI C'ACGA_G
Cuts at: 3016 3724 3016
Size: 708 4037
BstAPI GCAn_nnn'nTGC
Cuts at: 2122 2122
Size: 4745
BstBI TT'CG_AA
Cuts at: 1020 3106 1020
Size: 2086 2659
BstYI r'GATC_y
Cuts at: 1 1770 2237 2260 2595 2841 3147 3159 3245
Size: 1769 467 23 335 246 306 12 86
Cuts at: 3245 4297 1
Size: 1052 449
Fragments arranged by size:
1769 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2695 2876 3039 2695
Size: 181 163 4401
Fragments arranged by size:
4401 181 163
BtsI GCAGTG_nn'
Cuts at: 757 1005 1341 1988 757
Size: 248 336 647 3514
Fragments arranged by size:
3514 647 336 248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 819 854 903 938 1407 1442 819
Size: 35 49 35 469 35 4122
Fragments arranged by size:
4122 469 49 35 35 35
DraI TTT'AAA
Cuts at: 1744 1744
Size: 4745
DrdI GACnn_nn'nnGTC
Cuts at: 2451 3795 2451
Size: 1344 3401
EaeI y'GGCC_r
Cuts at: 2330 2504 2895 2922 4267 2330
Size: 174 391 27 1345 2808
Fragments arranged by size:
2808 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2330 2330
Size: 4745
EarI CTCTTCn'nnn_
Cuts at: 125 141 470 1017 2768 2978 125
Size: 16 329 547 1751 210 1892
Fragments arranged by size:
1892 1751 547 329 210 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2953 3695 3841 4555 2953
Size: 742 146 714 3143
Fragments arranged by size:
3143 742 714 146
EcoICRI GAG'CTC
Cuts at: 608 1757 608
Size: 1149 3596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 211 662 746 830 914 1250 1418 1502
Size: 62 451 84 84 84 336 168 84
Cuts at: 1502 1674 2570 3002 3355 4017 149
Size: 172 896 432 353 662 877
Fragments arranged by size:
896 877 662 451 432 353 336 172
168 84 84 84 84 62
EcoNI CCTnn'n_nnAGG
Cuts at: 340 340
Size: 4745
EcoO109I rG'GnC_Cy
Cuts at: 1884 1884
Size: 4745
EcoRI G'AATT_C
Cuts at: 716 800 843 884 968 1052 1136 1220 1472
Size: 84 43 41 84 84 84 84 252
Cuts at: 1472 1640 1749 716
Size: 168 109 3712
Fragments arranged by size:
3712 252 168 109 84 84 84 84
84 43 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 170 2764 170
Size: 2594 2151
FspI TGC'GCA
Cuts at: 2526 2526
Size: 4745
HaeII r_GCGC'y
Cuts at: 2179 2427 3657 4428 2179
Size: 248 1230 771 2496
Fragments arranged by size:
2496 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 471 503 2587 2617 2619 2649 471
Size: 32 2084 30 2 30 2567
Fragments arranged by size:
2567 2084 32 30 30 2
HincII GTy'rAC
Cuts at: 1784 1784
Size: 4745
HindIII A'AGCT_T
Cuts at: 426 426
Size: 4745
KasI G'GCGC_C
Cuts at: 2175 2423 2175
Size: 248 4497
KpnI G_GTAC'C
Cuts at: 1765 1765
Size: 4745
MluI A'CGCG_T
Cuts at: 4178 4178
Size: 4745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 568 3504 3688 568
Size: 2936 184 1625
Fragments arranged by size:
2936 1625 184
MscI TGG'CCA
Cuts at: 2506 2506
Size: 4745
MslI CAynn'nnrTG
Cuts at: 727 741 768 977 1079 1245 1565 1625 1649
Size: 14 27 209 102 166 320 60 24
Cuts at: 1649 1733 2734 2861 727
Size: 84 1001 127 2611
Fragments arranged by size:
2611 1001 320 209 166 127 102 84
60 27 24 14
MspA1I CmG'CkG
Cuts at: 1856 2170 2530 3314 3559 1856
Size: 314 360 784 245 3042
Fragments arranged by size:
3042 784 360 314 245
NaeI GCC'GGC
Cuts at: 2926 2926
Size: 4745
NarI GG'CG_CC
Cuts at: 2176 2424 2176
Size: 248 4497
NdeI CA'TA_TG
Cuts at: 738 994 1466 738
Size: 256 472 4017
Fragments arranged by size:
4017 472 256
NgoMIV G'CCGG_C
Cuts at: 2924 2924
Size: 4745
NsiI A_TGCA'T
Cuts at: 288 288
Size: 4745
NspI r_CATG'y
Cuts at: 290 662 1798 2298 3901 290
Size: 372 1136 500 1603 1134
Fragments arranged by size:
1603 1136 1134 500 372
PciI A'CATG_T
Cuts at: 658 3897 658
Size: 3239 1506
PfoI T'CCnGG_A
Cuts at: 341 341
Size: 4745
PmeI GTTT'AAAC
Cuts at: 1744 1744
Size: 4745
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4713
PsiI TTA'TAA
Cuts at: 824 908 824
Size: 84 4661
PstI C_TGCA'G
Cuts at: 1792 2477 1792
Size: 685 4060
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4745
PvuII CAG'CTG
Cuts at: 2170 2530 2170
Size: 360 4385
RsrII CG'GwC_CG
Cuts at: 2940 2940
Size: 4745
SacI G_AGCT'C
Cuts at: 610 1759 610
Size: 1149 3596
SalI G'TCGA_C
Cuts at: 1782 1782
Size: 4745
SapI GCTCTTCn'nnn_
Cuts at: 1017 2768 2978 1017
Size: 1751 210 2784
Fragments arranged by size:
2784 1751 210
SbfI CC_TGCA'GG
Cuts at: 1792 1792
Size: 4745
ScaI AGT'ACT
Cuts at: 4262 4262
Size: 4745
SfcI C'TryA_G
Cuts at: 33 470 1788 2473 3441 3632 33
Size: 437 1318 685 968 191 1146
Fragments arranged by size:
1318 1146 968 685 437 191
SfoI GGC'GCC
Cuts at: 2177 2425 2177
Size: 248 4497
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4745
SmaI CCC'GGG
Cuts at: 1767 1767
Size: 4745
SmlI C'TyrA_G
Cuts at: 441 1900 2815 3529 3791 441
Size: 1459 915 714 262 1395
Fragments arranged by size:
1459 1395 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4745
SphI G_CATG'C
Cuts at: 1798 2298 1798
Size: 500 4245
StyI C'CwwG_G
Cuts at: 313 1879 2233 313
Size: 1566 354 2825
Fragments arranged by size:
2825 1566 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2328 2461 2920 3129 2328
Size: 133 459 209 3944
Fragments arranged by size:
3944 459 209 133
TatI w'GTAC_w
Cuts at: 4260 4260
Size: 4745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4262 4294 4262
Size: 32 4713
Tth111I GACn'n_nGTC
Cuts at: 2542 2542
Size: 4745
XbaI T'CTAG_A
Cuts at: 1776 1776
Size: 4745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2240 2240
Size: 4745
XmaI C'CCGG_G
Cuts at: 1765 1765
Size: 4745
XmnI GAAnn'nnTTC
Cuts at: 847 931 1099 1603 847
Size: 84 168 504 3989
Fragments arranged by size:
3989 504 168 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BglI BmtI Bpu10I
BsaXI BseRI BsiWI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI