Restriction map for
pF1KB4782
(Circular) (Six-base) MAPSORT of: pF1KB4782.seq Check: 6211 from: 1 to: 4154
pF1KB4782 4154 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 406 406
Size: 4154
AccI GT'mk_AC
Cuts at: 1192 1192
Size: 4154
Acc65I G'GTAC_C
Cuts at: 1170 1170
Size: 4154
AclI AA'CG_TT
Cuts at: 3994 3994
Size: 4154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 783 1979 2411 2764 3426 448
Size: 335 1196 432 353 662 1176
Fragments arranged by size:
1196 1176 662 432 353 335
AflIII A'CryG_T
Cuts at: 740 848 1506 3306 3587 740
Size: 108 658 1800 281 1307
Fragments arranged by size:
1800 1307 658 281 108
AhdI GACnn_n'nnGTC
Cuts at: 900 900
Size: 4154
AlwNI CAG_nnn'CTG
Cuts at: 165 1459 2897 165
Size: 1294 1438 1422
Fragments arranged by size:
1438 1422 1294
ApaLI G'TGCA_C
Cuts at: 1105 2992 1105
Size: 1887 2267
ApoI r'AATT_y
Cuts at: 1158 3507 3763 1158
Size: 2349 256 1549
Fragments arranged by size:
2349 1549 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4154
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
AvaI C'yCGr_G
Cuts at: 176 1018 1174 176
Size: 842 156 3156
Fragments arranged by size:
3156 842 156
BamHI G'GATC_C
Cuts at: 863 1179 863
Size: 316 3838
BanI G'GyrC_C
Cuts at: 1170 1584 1832 1867 1170
Size: 414 248 35 3457
Fragments arranged by size:
3457 414 248 35
BanII G_rGCy'C
Cuts at: 946 1168 2198 946
Size: 222 1030 2902
Fragments arranged by size:
2902 1030 222
BbeI G_GCGC'C
Cuts at: 1588 1836 1588
Size: 248 3906
BbsI GAAGACnn'nnnn_
Cuts at: 421 557 421
Size: 136 4018
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4120
BciVI GTATCCnnnnn_n'
Cuts at: 1093 2052 3108 4154 1093
Size: 959 1056 1046 1093
Fragments arranged by size:
1093 1056 1046 959
BclI T'GATC_A
Cuts at: 608 1674 608
Size: 1066 3088
BfrBI ATG'CAT
Cuts at: 699 699
Size: 4154
BglII A'GATC_T
Cuts at: 440 1669 3706 440
Size: 1229 2037 888
Fragments arranged by size:
2037 1229 888
BlpI GC'TnA_GC
Cuts at: 1266 1266
Size: 4154
Bme1580I G_kGCm'C
Cuts at: 189 1109 1779 1872 2996 189
Size: 920 670 93 1124 1347
Fragments arranged by size:
1347 1124 920 670 93
BmrI ACTGGGnnnn_n'
Cuts at: 1366 1447 1782 3926 1366
Size: 81 335 2144 1594
Fragments arranged by size:
2144 1594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1047 2521 1047
Size: 1474 2680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 922 595
Size: 327 3827
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 2209 2923 3221 1330
Size: 879 714 298 2263
Fragments arranged by size:
2263 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 802 1080 3858 802
Size: 278 2778 1098
Fragments arranged by size:
2778 1098 278
BsaAI yAC'GTr
Cuts at: 1104 1507 1104
Size: 403 3751
BsaBI GATnn'nnATC
Cuts at: 6 1129 1668 3705 6
Size: 1123 539 2037 455
Fragments arranged by size:
2037 1123 539 455
BsaHI Gr'CG_yC
Cuts at: 458 761 838 1585 1833 2535 4031 458
Size: 303 77 747 248 702 1496 581
Fragments arranged by size:
1496 747 702 581 303 248 77
BsaWI w'CCGG_w
Cuts at: 855 1343 1864 2542 2953 3100 3374 855
Size: 488 521 678 411 147 274 1635
Fragments arranged by size:
1635 678 521 488 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4154
BseYI C'CCAG_C
Cuts at: 128 1579 2337 3002 3410 128
Size: 1451 758 665 408 872
Fragments arranged by size:
1451 872 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 691 699 457
Size: 234 8 3912
Fragments arranged by size:
3912 234 8
BsiEI CG_ry'CG
Cuts at: 104 1696 1742 2972 104
Size: 1592 46 1230 1286
Fragments arranged by size:
1592 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 695 1028 1109 1168 1946 2136 2996 695
Size: 333 81 59 778 190 860 1853
Fragments arranged by size:
1853 860 778 333 190 81 59
BsmI GAATG_Cn'
Cuts at: 699 699
Size: 4154
Bsp1286I G_dGCh'C
Cuts at: 189 695 946 1028 1109 1168 1779 1872 1946
Size: 506 251 82 81 59 611 93 74
Cuts at: 1946 2136 2198 2996 189
Size: 190 62 798 1347
Fragments arranged by size:
1347 798 611 506 251 190 93 82
81 74 62 59
BspHI T'CATG_A
Cuts at: 2586 2586
Size: 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 406 1204 1418 1720 2101 406
Size: 798 214 302 381 2459
Fragments arranged by size:
2459 798 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2446 2500 3984 4149 50
Size: 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2066 2066
Size: 4154
BsrFI r'CCGG_y
Cuts at: 2152 2333 2152
Size: 181 3973
BsrGI T'GTAC_A
Cuts at: 386 386
Size: 4154
BssSI C'ACGA_G
Cuts at: 190 2425 3133 190
Size: 2235 708 1211
Fragments arranged by size:
2235 1211 708
BstAPI GCAn_nnn'nTGC
Cuts at: 165 1531 165
Size: 1366 2788
BstBI TT'CG_AA
Cuts at: 2515 2515
Size: 4154
BstEII G'GTnAC_C
Cuts at: 775 775
Size: 4154
BstYI r'GATC_y
Cuts at: 1 440 863 1179 1646 1669 2004 2250 2556
Size: 439 423 316 467 23 335 246 306
Cuts at: 2556 2568 2654 3706 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 439 423 335 316 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 237 686 755 237
Size: 449 69 3636
Fragments arranged by size:
3636 449 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2104 2285 2448 2104
Size: 181 163 3810
Fragments arranged by size:
3810 181 163
BtsI GCAGTG_nn'
Cuts at: 1003 1397 1003
Size: 394 3760
DraI TTT'AAA
Cuts at: 1153 1153
Size: 4154
DraIII CAC_nnn'GTG
Cuts at: 1113 1113
Size: 4154
DrdI GACnn_nn'nnGTC
Cuts at: 1860 3204 1860
Size: 1344 2810
EaeI y'GGCC_r
Cuts at: 225 1115 1739 1913 2304 2331 3676 225
Size: 890 624 174 391 27 1345 703
Fragments arranged by size:
1345 890 703 624 391 174 27
EagI C'GGCC_G
Cuts at: 1739 1739
Size: 4154
EarI CTCTTCn'nnn_
Cuts at: 976 2177 2387 976
Size: 1201 210 2743
Fragments arranged by size:
2743 1201 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 352 2362 3104 3250 3964 352
Size: 2010 742 146 714 542
Fragments arranged by size:
2010 742 714 542 146
EcoICRI GAG'CTC
Cuts at: 1166 1166
Size: 4154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 595 783 922 1979 2411 2764 3426 448
Size: 147 188 139 1057 432 353 662 1176
Fragments arranged by size:
1176 1057 662 432 353 188 147 139
EcoO109I rG'GnC_Cy
Cuts at: 274 594 1293 274
Size: 320 699 3135
Fragments arranged by size:
3135 699 320
EcoRI G'AATT_C
Cuts at: 1158 1158
Size: 4154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4154
FspI TGC'GCA
Cuts at: 1935 1935
Size: 4154
HaeII r_GCGC'y
Cuts at: 686 1588 1836 3066 3837 686
Size: 902 248 1230 771 1003
Fragments arranged by size:
1230 1003 902 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 408 440 886 918 1037 1069 1079 1111 1996
Size: 32 446 32 119 32 10 32 885
Cuts at: 1996 2026 2028 2058 408
Size: 30 2 30 2504
Fragments arranged by size:
2504 885 446 119 32 32 32 32
30 30 10 2
HincII GTy'rAC
Cuts at: 1193 1193
Size: 4154
KasI G'GCGC_C
Cuts at: 1584 1832 1584
Size: 248 3906
KpnI G_GTAC'C
Cuts at: 1174 1174
Size: 4154
MluI A'CGCG_T
Cuts at: 3587 3587
Size: 4154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 917 2913 3097 275
Size: 642 1996 184 1332
Fragments arranged by size:
1996 1332 642 184
MscI TGG'CCA
Cuts at: 1117 1915 1117
Size: 798 3356
MslI CAynn'nnrTG
Cuts at: 1135 2143 2270 1135
Size: 1008 127 3019
Fragments arranged by size:
3019 1008 127
MspA1I CmG'CkG
Cuts at: 675 1265 1579 1939 2723 2968 675
Size: 590 314 360 784 245 1861
Fragments arranged by size:
1861 784 590 360 314 245
NaeI GCC'GGC
Cuts at: 2335 2335
Size: 4154
NarI GG'CG_CC
Cuts at: 1585 1833 1585
Size: 248 3906
NcoI C'CATG_G
Cuts at: 237 237
Size: 4154
NgoMIV G'CCGG_C
Cuts at: 2333 2333
Size: 4154
NsiI A_TGCA'T
Cuts at: 701 701
Size: 4154
NspI r_CATG'y
Cuts at: 206 234 699 852 1062 1207 1707 3310 206
Size: 28 465 153 210 145 500 1603 1050
Fragments arranged by size:
1603 1050 500 465 210 153 145 28
PasI CC'CwG_GG
Cuts at: 270 270
Size: 4154
PciI A'CATG_T
Cuts at: 848 3306 848
Size: 2458 1696
PflMI CCAn_nnn'nTGG
Cuts at: 270 270
Size: 4154
PfoI T'CCnGG_A
Cuts at: 641 641
Size: 4154
PmeI GTTT'AAAC
Cuts at: 1153 1153
Size: 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 4122
PpuMI rG'GwC_Cy
Cuts at: 594 594
Size: 4154
PshAI GACnn'nnGTC
Cuts at: 782 782
Size: 4154
PspXI vC'TCGA_Gb
Cuts at: 176 176
Size: 4154
PstI C_TGCA'G
Cuts at: 1201 1886 1201
Size: 685 3469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4154
PvuII CAG'CTG
Cuts at: 675 1579 1939 675
Size: 904 360 2890
Fragments arranged by size:
2890 904 360
RsrII CG'GwC_CG
Cuts at: 2349 2349
Size: 4154
SacI G_AGCT'C
Cuts at: 1168 1168
Size: 4154
SalI G'TCGA_C
Cuts at: 1191 1191
Size: 4154
SapI GCTCTTCn'nnn_
Cuts at: 2177 2387 2177
Size: 210 3944
SbfI CC_TGCA'GG
Cuts at: 1201 1201
Size: 4154
ScaI AGT'ACT
Cuts at: 616 664 3671 616
Size: 48 3007 1099
Fragments arranged by size:
3007 1099 48
SexAI A'CCwGG_T
Cuts at: 1109 1109
Size: 4154
SfcI C'TryA_G
Cuts at: 33 1197 1882 2850 3041 33
Size: 1164 685 968 191 1146
Fragments arranged by size:
1164 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1586 1834 1586
Size: 248 3906
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4154
SmaI CCC'GGG
Cuts at: 1020 1176 1020
Size: 156 3998
SmlI C'TyrA_G
Cuts at: 176 1309 2224 2938 3200 176
Size: 1133 915 714 262 1130
Fragments arranged by size:
1133 1130 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4154
SphI G_CATG'C
Cuts at: 206 1207 1707 206
Size: 1001 500 2653
Fragments arranged by size:
2653 1001 500
StuI AGG'CCT
Cuts at: 174 174
Size: 4154
StyI C'CwwG_G
Cuts at: 169 237 461 780 905 1288 1642 169
Size: 68 224 319 125 383 354 2681
Fragments arranged by size:
2681 383 354 319 224 125 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 530 1737 1870 2329 2538 530
Size: 1207 133 459 209 2146
Fragments arranged by size:
2146 1207 459 209 133
TatI w'GTAC_w
Cuts at: 386 614 662 3669 386
Size: 228 48 3007 871
Fragments arranged by size:
3007 871 228 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4122
Tth111I GACn'n_nGTC
Cuts at: 478 1951 478
Size: 1473 2681
XbaI T'CTAG_A
Cuts at: 1185 1185
Size: 4154
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 235 402 1649 235
Size: 167 1247 2740
Fragments arranged by size:
2740 1247 167
XhoI C'TCGA_G
Cuts at: 176 176
Size: 4154
XmaI C'CCGG_G
Cuts at: 1018 1174 1018
Size: 156 3998
XmnI GAAnn'nnTTC
Cuts at: 970 970
Size: 4154
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BspEI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PmlI PsiI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI