Restriction map for
pF1KB4783
(Circular) (Six-base) MAPSORT of: pF1KB4783.seq Check: 7614 from: 1 to: 5069
pF1KB4783 5069 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 645 645
Size: 5069
AatII G_ACGT'C
Cuts at: 783 783
Size: 5069
AccI GT'mk_AC
Cuts at: 2107 2107
Size: 5069
Acc65I G'GTAC_C
Cuts at: 1417 2085 1417
Size: 668 4401
AclI AA'CG_TT
Cuts at: 4909 4909
Size: 5069
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1574 2894 3326 4073 4341 1574
Size: 1320 432 747 268 2302
Fragments arranged by size:
2302 1320 747 432 268
AflIII A'CryG_T
Cuts at: 2421 3525 4502 2421
Size: 1104 977 2988
Fragments arranged by size:
2988 1104 977
AgeI A'CCGG_T
Cuts at: 3457 3457
Size: 5069
AleI CACnn'nnGTG
Cuts at: 930 930
Size: 5069
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 869
Size: 32 5037
AlwNI CAG_nnn'CTG
Cuts at: 927 1950 2374 3941 927
Size: 1023 424 1567 2055
Fragments arranged by size:
2055 1567 1023 424
ApaLI G'TGCA_C
Cuts at: 1378 3839 1378
Size: 2461 2608
ApoI r'AATT_y
Cuts at: 196 267 1743 2073 4422 4678 196
Size: 71 1476 330 2349 256 587
Fragments arranged by size:
2349 1476 587 330 256 71
AseI AT'TA_AT
Cuts at: 20 20
Size: 5069
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
AvaI C'yCGr_G
Cuts at: 2089 2089
Size: 5069
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1401 1434 1401
Size: 33 5036
BamHI G'GATC_C
Cuts at: 2094 2094
Size: 5069
BanI G'GyrC_C
Cuts at: 1275 1417 1789 1813 2085 2499 2747 2782 1275
Size: 142 372 24 272 414 248 35 3562
Fragments arranged by size:
3562 414 372 272 248 142 35 24
BanII G_rGCy'C
Cuts at: 251 1900 2083 3113 251
Size: 1649 183 1030 2207
Fragments arranged by size:
2207 1649 1030 183
BbeI G_GCGC'C
Cuts at: 2503 2751 2503
Size: 248 4821
BbsI GAAGACnn'nnnn_
Cuts at: 538 538
Size: 5069
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5035
BciVI GTATCCnnnnn_n'
Cuts at: 1222 2967 3728 5069 1222
Size: 1745 761 1341 1222
Fragments arranged by size:
1745 1341 1222 761
BclI T'GATC_A
Cuts at: 1340 2589 1340
Size: 1249 3820
BglI GCCn_nnn'nGGC
Cuts at: 918 1495 918
Size: 577 4492
BglII A'GATC_T
Cuts at: 758 2584 4621 758
Size: 1826 2037 1206
Fragments arranged by size:
2037 1826 1206
BlpI GC'TnA_GC
Cuts at: 2181 2181
Size: 5069
Bme1580I G_kGCm'C
Cuts at: 1382 2694 2787 3843 1382
Size: 1312 93 1056 2608
Fragments arranged by size:
2608 1312 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1889 2281 2362 2697 4841 1889
Size: 392 81 335 2144 2117
Fragments arranged by size:
2144 2117 392 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 5069
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1027 1036 817
Size: 210 9 4850
Fragments arranged by size:
4850 210 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2245 3124 3616 3914 1817
Size: 428 879 492 298 2972
Fragments arranged by size:
2972 879 492 428 298
BsaI GGTCTCn'nnnn_
Cuts at: 4773 4773
Size: 5069
BsaAI yAC'GTr
Cuts at: 2059 2422 2059
Size: 363 4706
BsaBI GATnn'nnATC
Cuts at: 6 2583 4620 6
Size: 2577 2037 455
Fragments arranged by size:
2577 2037 455
BsaHI Gr'CG_yC
Cuts at: 780 2500 2748 3450 4946 780
Size: 1720 248 702 1496 903
Fragments arranged by size:
1720 1496 903 702 248
BsaWI w'CCGG_w
Cuts at: 285 1577 2258 2779 3457 3731 3878 4289 285
Size: 1292 681 521 678 274 147 411 1065
Fragments arranged by size:
1292 1065 681 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 131 1550 1658 2021 2024 2027 2036 2039 131
Size: 1419 108 363 3 3 9 3 3161
Fragments arranged by size:
3161 1419 363 108 9 3 3 3
BseYI C'CCAG_C
Cuts at: 641 2494 3252 3829 4325 641
Size: 1853 758 577 496 1385
Fragments arranged by size:
1853 1385 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2611 2657 3865 104
Size: 2507 46 1208 1308
Fragments arranged by size:
2507 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 251 717 1382 1761 1900 2083 2861 3051 3843
Size: 466 665 379 139 183 778 190 792
Cuts at: 3843 251
Size: 1477
Fragments arranged by size:
1477 792 778 665 466 379 190 183
139
BsmI GAATG_Cn'
Cuts at: 863 863
Size: 5069
BsmBI CGTCTCn'nnnn_
Cuts at: 1149 1149
Size: 5069
Bsp1286I G_dGCh'C
Cuts at: 251 717 1382 1761 1900 2083 2694 2787 2861
Size: 466 665 379 139 183 611 93 74
Cuts at: 2861 3051 3113 3843 251
Size: 190 62 730 1477
Fragments arranged by size:
1477 730 665 611 466 379 190 183
139 93 74 62
BspEI T'CCGG_A
Cuts at: 285 4289 285
Size: 4004 1065
BspHI T'CATG_A
Cuts at: 4245 4245
Size: 5069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 645 2119 2333 2635 3016 645
Size: 1474 214 302 381 2698
Fragments arranged by size:
2698 1474 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3361 3415 4899 5064 50
Size: 3311 54 1484 165 55
Fragments arranged by size:
3311 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 509 2981 509
Size: 2472 2597
BsrFI r'CCGG_y
Cuts at: 1925 3067 3248 3457 1925
Size: 1142 181 209 3537
Fragments arranged by size:
3537 1142 209 181
BssSI C'ACGA_G
Cuts at: 3340 3698 3340
Size: 358 4711
BstAPI GCAn_nnn'nTGC
Cuts at: 2446 2446
Size: 5069
BstBI TT'CG_AA
Cuts at: 3430 3430
Size: 5069
BstYI r'GATC_y
Cuts at: 1 758 884 2094 2561 2584 2919 3165 4177
Size: 757 126 1210 467 23 335 246 1012
Cuts at: 4177 4263 4275 4621 1
Size: 86 12 346 449
Fragments arranged by size:
1210 1012 757 467 449 346 335 246
126 86 23 12
BtgI C'CryG_G
Cuts at: 150 1215 1308 150
Size: 1065 93 3911
Fragments arranged by size:
3911 1065 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3019 3200 3363 3019
Size: 181 163 4725
Fragments arranged by size:
4725 181 163
BtsI GCAGTG_nn'
Cuts at: 1731 2312 1731
Size: 581 4488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1638 1673 1638
Size: 35 5034
DraI TTT'AAA
Cuts at: 2068 2068
Size: 5069
DrdI GACnn_nn'nnGTC
Cuts at: 2775 3633 2775
Size: 858 4211
EaeI y'GGCC_r
Cuts at: 492 659 946 1198 1389 1659 2654 2828 3219
Size: 167 287 252 191 270 995 174 391
Cuts at: 3219 3246 4591 492
Size: 27 1345 970
Fragments arranged by size:
1345 995 970 391 287 270 252 191
174 167 27
EagI C'GGCC_G
Cuts at: 2654 2654
Size: 5069
EarI CTCTTCn'nnn_
Cuts at: 685 1478 3092 3302 685
Size: 793 1614 210 2452
Fragments arranged by size:
2452 1614 793 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1559 3277 3587 3733 4879 1559
Size: 1718 310 146 1146 1749
Fragments arranged by size:
1749 1718 1146 310 146
EcoICRI GAG'CTC
Cuts at: 249 1898 2081 249
Size: 1649 183 3237
Fragments arranged by size:
3237 1649 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1027 1036 1574 2894 3326 4073 4341 817
Size: 210 9 538 1320 432 747 268 1545
Fragments arranged by size:
1545 1320 747 538 432 268 210 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1453 1453
Size: 5069
EcoO109I rG'GnC_Cy
Cuts at: 724 2208 724
Size: 1484 3585
EcoRI G'AATT_C
Cuts at: 2073 2073
Size: 5069
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5069
FspI TGC'GCA
Cuts at: 1186 2850 1186
Size: 1664 3405
HaeII r_GCGC'y
Cuts at: 2503 2751 3773 4752 2503
Size: 248 1022 979 2820
Fragments arranged by size:
2820 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1288 1320 1454 1486 2911 2941 2943 2973 1288
Size: 32 134 32 1425 30 2 30 3384
Fragments arranged by size:
3384 1425 134 32 32 30 30 2
HincII GTy'rAC
Cuts at: 900 1231 2108 900
Size: 331 877 3861
Fragments arranged by size:
3861 877 331
KasI G'GCGC_C
Cuts at: 2499 2747 2499
Size: 248 4821
KpnI G_GTAC'C
Cuts at: 1421 2089 1421
Size: 668 4401
MfeI C'AATT_G
Cuts at: 557 557
Size: 5069
MluI A'CGCG_T
Cuts at: 4502 4502
Size: 5069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1116 3740 3924 1116
Size: 2624 184 2261
Fragments arranged by size:
2624 2261 184
MscI TGG'CCA
Cuts at: 948 1200 2830 948
Size: 252 1630 3187
Fragments arranged by size:
3187 1630 252
MslI CAynn'nnrTG
Cuts at: 930 3058 3185 930
Size: 2128 127 2814
Fragments arranged by size:
2814 2128 127
MspA1I CmG'CkG
Cuts at: 1804 2180 2494 2854 3867 4112 1804
Size: 376 314 360 1013 245 2761
Fragments arranged by size:
2761 1013 376 360 314 245
NaeI GCC'GGC
Cuts at: 3250 3250
Size: 5069
NarI GG'CG_CC
Cuts at: 2500 2748 2500
Size: 248 4821
NcoI C'CATG_G
Cuts at: 1215 1215
Size: 5069
NdeI CA'TA_TG
Cuts at: 309 309
Size: 5069
NgoMIV G'CCGG_C
Cuts at: 3248 3248
Size: 5069
NspI r_CATG'y
Cuts at: 2122 2622 3529 2122
Size: 500 907 3662
Fragments arranged by size:
3662 907 500
PasI CC'CwG_GG
Cuts at: 1441 1441
Size: 5069
PciI A'CATG_T
Cuts at: 3525 3525
Size: 5069
PflMI CCAn_nnn'nTGG
Cuts at: 1050 1098 1050
Size: 48 5021
PfoI T'CCnGG_A
Cuts at: 774 794 1977 774
Size: 20 1183 3866
Fragments arranged by size:
3866 1183 20
PmeI GTTT'AAAC
Cuts at: 2068 2068
Size: 5069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 5037
PpuMI rG'GwC_Cy
Cuts at: 724 724
Size: 5069
PshAI GACnn'nnGTC
Cuts at: 1226 1226
Size: 5069
PstI C_TGCA'G
Cuts at: 2116 2801 2116
Size: 685 4384
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5069
PvuII CAG'CTG
Cuts at: 1804 2494 2854 1804
Size: 690 360 4019
Fragments arranged by size:
4019 690 360
RsrII CG'GwC_CG
Cuts at: 3264 3264
Size: 5069
SacI G_AGCT'C
Cuts at: 251 1900 2083 251
Size: 1649 183 3237
Fragments arranged by size:
3237 1649 183
SalI G'TCGA_C
Cuts at: 2106 2106
Size: 5069
SapI GCTCTTCn'nnn_
Cuts at: 3092 3302 3092
Size: 210 4859
SbfI CC_TGCA'GG
Cuts at: 2116 2116
Size: 5069
ScaI AGT'ACT
Cuts at: 696 4586 696
Size: 3890 1179
SfcI C'TryA_G
Cuts at: 33 1292 2112 2797 3790 3981 33
Size: 1259 820 685 993 191 1121
Fragments arranged by size:
1259 1121 993 820 685 191
SfoI GGC'GCC
Cuts at: 2501 2749 2501
Size: 248 4821
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5069
SmaI CCC'GGG
Cuts at: 2091 2091
Size: 5069
SmlI C'TyrA_G
Cuts at: 1832 2224 3139 3631 3893 1832
Size: 392 915 492 262 3008
Fragments arranged by size:
3008 915 492 392 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5069
SphI G_CATG'C
Cuts at: 2122 2622 2122
Size: 500 4569
SspI AAT'ATT
Cuts at: 1139 1139
Size: 5069
StuI AGG'CCT
Cuts at: 912 912
Size: 5069
StyI C'CwwG_G
Cuts at: 1215 2203 2557 1215
Size: 988 354 3727
Fragments arranged by size:
3727 988 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1009 1293 1646 2652 2785 3244 3453 1009
Size: 284 353 1006 133 459 209 2625
Fragments arranged by size:
2625 1006 459 353 284 209 133
TatI w'GTAC_w
Cuts at: 648 694 826 4584 648
Size: 46 132 3758 1133
Fragments arranged by size:
3758 1133 132 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4586 4618 4586
Size: 32 5037
Tth111I GACn'n_nGTC
Cuts at: 2866 2866
Size: 5069
XbaI T'CTAG_A
Cuts at: 682 2100 682
Size: 1418 3651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1241 1777 1912 2564 1241
Size: 536 135 652 3746
Fragments arranged by size:
3746 652 536 135
XmaI C'CCGG_G
Cuts at: 2089 2089
Size: 5069
ZraI GAC'GTC
Cuts at: 781 781
Size: 5069
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI ApaI AscI AvrII BbvCI
BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI