Restriction map for
pF1KB4802
(Circular) (Six-base) MAPSORT of: pF1KB4802.seq Check: 1090 from: 1 to: 6629
pF1KB4802 6629 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2598 3625 2598
Size: 1027 5602
AccI GT'mk_AC
Cuts at: 1908 3667 1908
Size: 1759 4870
Acc65I G'GTAC_C
Cuts at: 3645 3645
Size: 6629
AclI AA'CG_TT
Cuts at: 6469 6469
Size: 6629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 4454 4886 5633 5901 2225
Size: 2229 432 747 268 2953
Fragments arranged by size:
2953 2229 747 432 268
AfeI AGC'GCT
Cuts at: 511 982 1333 1644 2713 511
Size: 471 351 311 1069 4427
Fragments arranged by size:
4427 1069 471 351 311
AflIII A'CryG_T
Cuts at: 1078 3981 5085 6062 1078
Size: 2903 1104 977 1645
Fragments arranged by size:
2903 1645 1104 977
AgeI A'CCGG_T
Cuts at: 3377 5017 3377
Size: 1640 4989
AleI CACnn'nnGTG
Cuts at: 922 1397 922
Size: 475 6154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 977 2990 977
Size: 2013 4616
AlwNI CAG_nnn'CTG
Cuts at: 552 633 1896 1945 2100 2887 3171 3934 5501
Size: 81 1263 49 155 787 284 763 1567
Cuts at: 5501 552
Size: 1680
Fragments arranged by size:
1680 1567 1263 787 763 284 155 81
49
ApaI G_GGCC'C
Cuts at: 485 1738 1793 485
Size: 1253 55 5321
Fragments arranged by size:
5321 1253 55
ApaLI G'TGCA_C
Cuts at: 986 5399 986
Size: 4413 2216
ApoI r'AATT_y
Cuts at: 728 3633 5982 6238 728
Size: 2905 2349 256 1119
Fragments arranged by size:
2905 2349 1119 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6629
AvaI C'yCGr_G
Cuts at: 581 1209 1737 3649 581
Size: 628 528 1912 3561
Fragments arranged by size:
3561 1912 628 528
BamHI G'GATC_C
Cuts at: 1976 3419 3654 1976
Size: 1443 235 4951
Fragments arranged by size:
4951 1443 235
BanI G'GyrC_C
Cuts at: 623 1205 2389 3115 3304 3412 3645 4059 4307
Size: 582 1184 726 189 108 233 414 248
Cuts at: 4307 4342 623
Size: 35 2910
Fragments arranged by size:
2910 1184 726 582 414 248 233 189
108 35
BanII G_rGCy'C
Cuts at: 485 1278 1738 1793 3027 3245 3643 4673 485
Size: 793 460 55 1234 218 398 1030 2441
Fragments arranged by size:
2441 1234 1030 793 460 398 218 55
BbeI G_GCGC'C
Cuts at: 1209 4063 4311 1209
Size: 2854 248 3527
Fragments arranged by size:
3527 2854 248
BbsI GAAGACnn'nnnn_
Cuts at: 2423 3439 2423
Size: 1016 5613
BbvCI CC'TCA_GC
Cuts at: 1573 2999 3005 3155 1573
Size: 1426 6 150 5047
Fragments arranged by size:
5047 1426 150 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 601 635 2665 2699 2803 2837 601
Size: 34 2030 34 104 34 4393
Fragments arranged by size:
4393 2030 104 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4527 5288 6629 4527
Size: 761 1341 4527
Fragments arranged by size:
4527 1341 761
BclI T'GATC_A
Cuts at: 4149 4149
Size: 6629
BglI GCCn_nnn'nGGC
Cuts at: 642 1778 1814 2587 2857 642
Size: 1136 36 773 270 4414
Fragments arranged by size:
4414 1136 773 270 36
BglII A'GATC_T
Cuts at: 4144 6181 4144
Size: 2037 4592
BlpI GC'TnA_GC
Cuts at: 2260 2294 2615 3537 3741 2260
Size: 34 321 922 204 5148
Fragments arranged by size:
5148 922 321 204 34
Bme1580I G_kGCm'C
Cuts at: 485 791 990 1070 1609 1738 1793 2709 2985
Size: 306 199 80 539 129 55 916 276
Cuts at: 2985 3330 3417 4254 4347 5403 485
Size: 345 87 837 93 1056 1711
Fragments arranged by size:
1711 1056 916 837 539 345 306 276
199 129 93 87 80 55
BmgBI CAC'GTC
Cuts at: 1686 1686
Size: 6629
BmrI ACTGGGnnnn_n'
Cuts at: 273 1282 3841 3922 4257 6401 273
Size: 1009 2559 81 335 2144 501
Fragments arranged by size:
2559 2144 1009 501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 531 2035 4996 531
Size: 1504 2961 2164
Fragments arranged by size:
2961 2164 1504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 1032 1972 2077 2140 2173 2443 2463 2629
Size: 729 940 105 63 33 270 20 166
Cuts at: 2629 3577 303
Size: 948 3355
Fragments arranged by size:
3355 948 940 729 270 166 105 63
33 20
Bpu10I CC'TnA_GC
Cuts at: 1573 2054 2332 2999 3005 3098 3155 1573
Size: 481 278 667 6 93 57 5047
Fragments arranged by size:
5047 667 481 278 93 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 3805 4684 5176 5474 777
Size: 3028 879 492 298 1932
Fragments arranged by size:
3028 1932 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 105 259 1008 1222 2141 2185 2307 2713 3454
Size: 154 749 214 919 44 122 406 741
Cuts at: 3454 6333 105
Size: 2879 401
Fragments arranged by size:
2879 919 749 741 406 401 214 154
122 44
BsaAI yAC'GTr
Cuts at: 3982 3982
Size: 6629
BsaBI GATnn'nnATC
Cuts at: 6 4143 6180 6
Size: 4137 2037 455
Fragments arranged by size:
4137 2037 455
BsaHI Gr'CG_yC
Cuts at: 1034 1206 4060 4308 5010 6506 1034
Size: 172 2854 248 702 1496 1157
Fragments arranged by size:
2854 1496 1157 702 248 172
BsaWI w'CCGG_w
Cuts at: 1196 1254 1670 2252 3377 3818 4339 5017 5291
Size: 58 416 582 1125 441 521 678 274
Cuts at: 5291 5438 5849 1196
Size: 147 411 1976
Fragments arranged by size:
1976 1125 678 582 521 441 416 411
274 147 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 2116 2119 2146 2149 553
Size: 30 1533 3 27 3 5033
Fragments arranged by size:
5033 1533 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 504 1459 2137 2140 2393 2396 2399 3253 3460
Size: 955 678 3 253 3 3 854 207
Cuts at: 3460 504
Size: 3673
Fragments arranged by size:
3673 955 854 678 253 207 3 3
3
BseYI C'CCAG_C
Cuts at: 811 833 2639 2708 3213 3287 3399 4054 4812
Size: 22 1806 69 505 74 112 655 758
Cuts at: 4812 5389 5885 811
Size: 577 496 1555
Fragments arranged by size:
1806 1555 758 655 577 505 496 112
74 69 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 549 946 1171 1342 1659 258
Size: 291 397 225 171 317 5228
Fragments arranged by size:
5228 397 317 291 225 171
BsiEI CG_ry'CG
Cuts at: 104 4171 4217 5425 104
Size: 4067 46 1208 1308
Fragments arranged by size:
4067 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 990 1506 2266 3245 3605 3643 4421 4611 5403
Size: 516 760 979 360 38 778 190 792
Cuts at: 5403 990
Size: 2216
Fragments arranged by size:
2216 979 792 778 760 516 360 190
38
BsmI GAATG_Cn'
Cuts at: 1048 1048
Size: 6629
BsmBI CGTCTCn'nnnn_
Cuts at: 1676 2122 1676
Size: 446 6183
Bsp1286I G_dGCh'C
Cuts at: 485 791 990 1070 1278 1506 1609 1738 1793
Size: 306 199 80 208 228 103 129 55
Cuts at: 1793 2266 2709 2985 3027 3245 3330 3417 3605
Size: 473 443 276 42 218 85 87 188
Cuts at: 3605 3643 4254 4347 4421 4611 4673 5403 485
Size: 38 611 93 74 190 62 730 1711
Fragments arranged by size:
1711 730 611 473 443 306 276 228
218 208 199 190 188 129 103 93
87 85 80 74 62 55 42 38
BspEI T'CCGG_A
Cuts at: 1196 1254 2252 5849 1196
Size: 58 998 3597 1976
Fragments arranged by size:
3597 1976 998 58
BspHI T'CATG_A
Cuts at: 464 5805 464
Size: 5341 1288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 918 1240 2289 2598 2778 3074 3156 3625
Size: 706 322 1049 309 180 296 82 469
Cuts at: 3625 3679 3893 4195 4576 212
Size: 54 214 302 381 2265
Fragments arranged by size:
2265 1049 706 469 381 322 309 302
296 214 180 82 54
BsrBI CCG'CTC
Cuts at: 50 1176 2741 3125 4921 4975 6459 6624 50
Size: 1126 1565 384 1796 54 1484 165 55
Fragments arranged by size:
1796 1565 1484 1126 384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1068 1923 2918 2972 4541 1068
Size: 855 995 54 1569 3156
Fragments arranged by size:
3156 1569 995 855 54
BsrFI r'CCGG_y
Cuts at: 493 964 1004 1017 1202 2392 2581 2987 3377
Size: 471 40 13 185 1190 189 406 390
Cuts at: 3377 4627 4808 5017 493
Size: 1250 181 209 2105
Fragments arranged by size:
2105 1250 1190 471 406 390 209 189
185 181 40 13
BssSI C'ACGA_G
Cuts at: 4900 5258 4900
Size: 358 6271
BstAPI GCAn_nnn'nTGC
Cuts at: 1241 1896 3171 3325 4006 1241
Size: 655 1275 154 681 3864
Fragments arranged by size:
3864 1275 681 655 154
BstBI TT'CG_AA
Cuts at: 4990 4990
Size: 6629
BstXI CCAn_nnnn'nTGG
Cuts at: 1479 2409 2487 2536 3213 3592 1479
Size: 930 78 49 677 379 4516
Fragments arranged by size:
4516 930 677 379 78 49
BstYI r'GATC_y
Cuts at: 1 1976 2006 2109 2909 3419 3575 3654 4121
Size: 1975 30 103 800 510 156 79 467
Cuts at: 4121 4144 4479 4725 5737 5823 5835 6181 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1975 1012 800 510 467 449 346 335
246 156 103 86 79 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 2080 2416 2080
Size: 336 6293
BtgI C'CryG_G
Cuts at: 174 1959 3464 174
Size: 1785 1505 3339
Fragments arranged by size:
3339 1785 1505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4579 4760 4923 4579
Size: 181 163 6285
Fragments arranged by size:
6285 181 163
BtsI GCAGTG_nn'
Cuts at: 959 1271 1306 1609 2235 3038 3085 3872 959
Size: 312 35 303 626 803 47 787 3716
Fragments arranged by size:
3716 803 787 626 312 303 47 35
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2451 2486 2451
Size: 35 6594
DraI TTT'AAA
Cuts at: 3628 3628
Size: 6629
DrdI GACnn_nn'nnGTC
Cuts at: 4335 5193 4335
Size: 858 5771
EaeI y'GGCC_r
Cuts at: 867 1523 1530 1688 2060 3175 4214 4388 4779
Size: 656 7 158 372 1115 1039 174 391
Cuts at: 4779 4806 6151 867
Size: 27 1345 1345
Fragments arranged by size:
1345 1345 1115 1039 656 391 372 174
158 27 7
EagI C'GGCC_G
Cuts at: 4214 4214
Size: 6629
EarI CTCTTCn'nnn_
Cuts at: 2197 4652 4862 2197
Size: 2455 210 3964
Fragments arranged by size:
3964 2455 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 577 1036 1645 3235 4837 5147 5293 6439 577
Size: 459 609 1590 1602 310 146 1146 767
Fragments arranged by size:
1602 1590 1146 767 609 459 310 146
EcoICRI GAG'CTC
Cuts at: 3243 3641 3243
Size: 398 6231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 1032 1972 2077 2140 2173 2225 2443 2463
Size: 729 940 105 63 33 52 218 20
Cuts at: 2463 2629 3577 4454 4886 5633 5901 303
Size: 166 948 877 432 747 268 1031
Fragments arranged by size:
1031 948 940 877 747 729 432 268
218 166 105 63 52 33 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2122 3070 2122
Size: 948 5681
EcoO109I rG'GnC_Cy
Cuts at: 179 201 218 481 899 910 1418 1483 1734
Size: 22 17 263 418 11 508 65 251
Cuts at: 1734 1790 1893 1942 2097 2588 3768 179
Size: 56 103 49 155 491 1180 3040
Fragments arranged by size:
3040 1180 508 491 418 263 251 155
103 65 56 49 22 17 11
EcoRI G'AATT_C
Cuts at: 3633 3633
Size: 6629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1371 4648 1371
Size: 3277 3352
FspI TGC'GCA
Cuts at: 885 4410 885
Size: 3525 3104
HaeII r_GCGC'y
Cuts at: 513 818 984 1209 1335 1458 1646 2715 2735
Size: 305 166 225 126 123 188 1069 20
Cuts at: 2735 4063 4311 5333 6312 513
Size: 1328 248 1022 979 830
Fragments arranged by size:
1328 1069 1022 979 830 305 248 225
188 166 126 123 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 682 714 1738 1770 2119 2151 4471
Size: 32 97 32 1024 32 349 32 2320
Cuts at: 4471 4501 4503 4533 553
Size: 30 2 30 2649
Fragments arranged by size:
2649 2320 1024 349 97 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3668 3668
Size: 6629
HindIII A'AGCT_T
Cuts at: 2169 2404 2169
Size: 235 6394
KasI G'GCGC_C
Cuts at: 1205 4059 4307 1205
Size: 2854 248 3527
Fragments arranged by size:
3527 2854 248
KpnI G_GTAC'C
Cuts at: 3649 3649
Size: 6629
MfeI C'AATT_G
Cuts at: 1140 1140
Size: 6629
MluI A'CGCG_T
Cuts at: 6062 6062
Size: 6629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 699 2330 3479 5300 5484 699
Size: 1631 1149 1821 184 1844
Fragments arranged by size:
1844 1821 1631 1149 184
MscI TGG'CCA
Cuts at: 1525 1532 1690 2062 4390 1525
Size: 7 158 372 2328 3764
Fragments arranged by size:
3764 2328 372 158 7
MslI CAynn'nnrTG
Cuts at: 869 922 1397 3469 3590 4618 4745 869
Size: 53 475 2072 121 1028 127 2753
Fragments arranged by size:
2753 2072 1028 475 127 121 53
MspA1I CmG'CkG
Cuts at: 429 922 1096 1185 1397 1763 1796 2106 2558
Size: 493 174 89 212 366 33 310 452
Cuts at: 2558 2622 2649 3213 3541 3740 4054 4414 5427
Size: 64 27 564 328 199 314 360 1013
Cuts at: 5427 5672 429
Size: 245 1386
Fragments arranged by size:
1386 1013 564 493 452 366 360 328
314 310 245 212 199 174 89 64
33 27
NaeI GCC'GGC
Cuts at: 495 966 4810 495
Size: 471 3844 2314
Fragments arranged by size:
3844 2314 471
NarI GG'CG_CC
Cuts at: 1206 4060 4308 1206
Size: 2854 248 3527
Fragments arranged by size:
3527 2854 248
NcoI C'CATG_G
Cuts at: 3464 3464
Size: 6629
NdeI CA'TA_TG
Cuts at: 1122 1122
Size: 6629
NgoMIV G'CCGG_C
Cuts at: 493 964 4808 493
Size: 471 3844 2314
Fragments arranged by size:
3844 2314 471
NspI r_CATG'y
Cuts at: 157 501 1082 1321 1434 3682 4182 5089 157
Size: 344 581 239 113 2248 500 907 1697
Fragments arranged by size:
2248 1697 907 581 500 344 239 113
PasI CC'CwG_GG
Cuts at: 2576 2576
Size: 6629
PciI A'CATG_T
Cuts at: 1078 5085 1078
Size: 4007 2622
PflMI CCAn_nnn'nTGG
Cuts at: 196 386 552 1934 2715 3033 196
Size: 190 166 1382 781 318 3792
Fragments arranged by size:
3792 1382 781 318 190 166
PfoI T'CCnGG_A
Cuts at: 1420 1883 1972 2792 2834 3140 1420
Size: 463 89 820 42 306 4909
Fragments arranged by size:
4909 820 463 306 89 42
PmeI GTTT'AAAC
Cuts at: 3628 3628
Size: 6629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 403 435 5768 5800 403
Size: 32 5333 32 1232
Fragments arranged by size:
5333 1232 32 32
PpuMI rG'GwC_Cy
Cuts at: 201 218 1418 1483 201
Size: 17 1200 65 5347
Fragments arranged by size:
5347 1200 65 17
PspOMI G'GGCC_C
Cuts at: 481 1734 1789 481
Size: 1253 55 5321
Fragments arranged by size:
5321 1253 55
PstI C_TGCA'G
Cuts at: 428 848 909 1650 2105 2563 2897 3092 3676
Size: 420 61 741 455 458 334 195 584
Cuts at: 3676 4361 428
Size: 685 2696
Fragments arranged by size:
2696 741 685 584 458 455 420 334
195 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6629
PvuII CAG'CTG
Cuts at: 429 1185 2106 2622 3213 4054 4414 429
Size: 756 921 516 591 841 360 2644
Fragments arranged by size:
2644 921 841 756 591 516 360
RsrII CG'GwC_CG
Cuts at: 4824 4824
Size: 6629
SacI G_AGCT'C
Cuts at: 3245 3643 3245
Size: 398 6231
SalI G'TCGA_C
Cuts at: 3666 3666
Size: 6629
SanDI GG'GwC_CC
Cuts at: 1418 1483 1418
Size: 65 6564
SapI GCTCTTCn'nnn_
Cuts at: 4652 4862 4652
Size: 210 6419
SbfI CC_TGCA'GG
Cuts at: 3676 3676
Size: 6629
ScaI AGT'ACT
Cuts at: 6146 6146
Size: 6629
SfcI C'TryA_G
Cuts at: 33 339 424 844 905 1646 2101 2559 2893
Size: 306 85 420 61 741 455 458 334
Cuts at: 2893 3088 3672 4357 5350 5541 33
Size: 195 584 685 993 191 1121
Fragments arranged by size:
1121 993 741 685 584 458 455 420
334 306 195 191 85 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 2587 2857 2587
Size: 270 6359
SfoI GGC'GCC
Cuts at: 1207 4061 4309 1207
Size: 2854 248 3527
Fragments arranged by size:
3527 2854 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6629
SmaI CCC'GGG
Cuts at: 1211 1739 3651 1211
Size: 528 1912 4189
Fragments arranged by size:
4189 1912 528
SmlI C'TyrA_G
Cuts at: 756 3784 4699 5191 5453 756
Size: 3028 915 492 262 1932
Fragments arranged by size:
3028 1932 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6629
SphI G_CATG'C
Cuts at: 157 501 3682 4182 157
Size: 344 3181 500 2604
Fragments arranged by size:
3181 2604 500 344
SrfI GCCC'GGGC
Cuts at: 1739 1739
Size: 6629
StuI AGG'CCT
Cuts at: 645 645
Size: 6629
StyI C'CwwG_G
Cuts at: 3464 3763 4117 3464
Size: 299 354 5976
Fragments arranged by size:
5976 354 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 903 2466 3205 3391 4212 4345 4804 5013
Size: 611 1563 739 186 821 133 459 209
Cuts at: 5013 292
Size: 1908
Fragments arranged by size:
1908 1563 821 739 611 459 209 186
133
TatI w'GTAC_w
Cuts at: 6144 6144
Size: 6629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 1420 1452 3411 3443 6146 6178 607
Size: 32 781 32 1959 32 2703 32 1058
Fragments arranged by size:
2703 1959 1058 781 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2133 4426 2133
Size: 2293 4336
XbaI T'CTAG_A
Cuts at: 3660 3660
Size: 6629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1120 2943 3471 4124 1120
Size: 1823 528 653 3625
Fragments arranged by size:
3625 1823 653 528
XmaI C'CCGG_G
Cuts at: 1209 1737 3649 1209
Size: 528 1912 4189
Fragments arranged by size:
4189 1912 528
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BfrBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HpaI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SexAI
SgrAI SnaBI SspI SwaI XhoI XmnI ZraI