Restriction map for
pF1KB4806
(Circular) (Six-base) MAPSORT of: pF1KB4806.seq Check: 8730 from: 1 to: 6524
pF1KB4806 6524 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1362 1362
Size: 6524
AccI GT'mk_AC
Cuts at: 1335 3562 1335
Size: 2227 4297
Acc65I G'GTAC_C
Cuts at: 275 574 3540 275
Size: 299 2966 3259
Fragments arranged by size:
3259 2966 299
AclI AA'CG_TT
Cuts at: 6364 6364
Size: 6524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1003 2152 3004 4349 4781 5528 5796 1003
Size: 1149 852 1345 432 747 268 1731
Fragments arranged by size:
1731 1345 1149 852 747 432 268
AfeI AGC'GCT
Cuts at: 1107 1107
Size: 6524
AflIII A'CryG_T
Cuts at: 3062 3876 4980 5957 3062
Size: 814 1104 977 3629
Fragments arranged by size:
3629 1104 977 814
AgeI A'CCGG_T
Cuts at: 571 4912 571
Size: 4341 2183
AlwNI CAG_nnn'CTG
Cuts at: 541 826 981 1025 1227 1488 1659 1764 2109
Size: 285 155 44 202 261 171 105 345
Cuts at: 2109 2668 3105 3448 3829 5396 541
Size: 559 437 343 381 1567 1669
Fragments arranged by size:
1669 1567 559 437 381 345 343 285
261 202 171 155 105 44
ApaI G_GGCC'C
Cuts at: 1156 1938 2477 1156
Size: 782 539 5203
Fragments arranged by size:
5203 782 539
ApaLI G'TGCA_C
Cuts at: 5294 5294
Size: 6524
ApoI r'AATT_y
Cuts at: 129 3528 5877 6133 129
Size: 3399 2349 256 520
Fragments arranged by size:
3399 2349 520 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6524
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
AvaI C'yCGr_G
Cuts at: 170 296 528 548 1192 3544 170
Size: 126 232 20 644 2352 3150
Fragments arranged by size:
3150 2352 644 232 126 20
AvrII C'CTAG_G
Cuts at: 3073 3073
Size: 6524
BamHI G'GATC_C
Cuts at: 959 1540 3549 959
Size: 581 2009 3934
Fragments arranged by size:
3934 2009 581
BanI G'GyrC_C
Cuts at: 275 317 574 735 3540 3954 4202 4237 275
Size: 42 257 161 2805 414 248 35 2562
Fragments arranged by size:
2805 2562 414 257 248 161 42 35
BanII G_rGCy'C
Cuts at: 679 902 943 1007 1072 1156 1938 2477 3538
Size: 223 41 64 65 84 782 539 1061
Cuts at: 3538 4568 679
Size: 1030 2635
Fragments arranged by size:
2635 1061 1030 782 539 223 84 65
64 41
BbeI G_GCGC'C
Cuts at: 3958 4206 3958
Size: 248 6276
BbsI GAAGACnn'nnnn_
Cuts at: 1060 1898 2641 2656 3065 1060
Size: 838 743 15 409 4519
Fragments arranged by size:
4519 838 743 409 15
BbvCI CC'TCA_GC
Cuts at: 264 1900 264
Size: 1636 4888
BciVI GTATCCnnnnn_n'
Cuts at: 4422 5183 6524 4422
Size: 761 1341 4422
Fragments arranged by size:
4422 1341 761
BclI T'GATC_A
Cuts at: 4044 4044
Size: 6524
BfrBI ATG'CAT
Cuts at: 1471 1471
Size: 6524
BglI GCCn_nnn'nGGC
Cuts at: 551 2513 551
Size: 1962 4562
BglII A'GATC_T
Cuts at: 1587 4039 6076 1587
Size: 2452 2037 2035
Fragments arranged by size:
2452 2037 2035
BlpI GC'TnA_GC
Cuts at: 942 952 1853 3636 942
Size: 10 901 1783 3830
Fragments arranged by size:
3830 1783 901 10
Bme1580I G_kGCm'C
Cuts at: 1156 1938 2477 3227 4149 4242 5298 1156
Size: 782 539 750 922 93 1056 2382
Fragments arranged by size:
2382 1056 922 782 750 539 93
BmrI ACTGGGnnnn_n'
Cuts at: 1319 2331 2423 3736 3817 4152 6296 1319
Size: 1012 92 1313 81 335 2144 1547
Fragments arranged by size:
2144 1547 1313 1012 335 92 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 206 862 2091 3144 4891 206
Size: 656 1229 1053 1747 1839
Fragments arranged by size:
1839 1747 1229 1053 656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 273 895 1465 1863 2179 2185 2194 2734
Size: 79 622 570 398 316 6 9 540
Cuts at: 2734 2812 2820 2949 3115 3127 194
Size: 78 8 129 166 12 3591
Fragments arranged by size:
3591 622 570 540 398 316 166 129
79 78 12 9 8 6
Bpu10I CC'TnA_GC
Cuts at: 264 1900 264
Size: 1636 4888
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 332 2206 2691 3346 3700 4579 5071 5369
Size: 40 1874 485 655 354 879 492 298
Cuts at: 5369 292
Size: 1447
Fragments arranged by size:
1874 1447 879 655 492 485 354 298
40
BsaI GGTCTCn'nnnn_
Cuts at: 194 1398 2386 3208 6228 194
Size: 1204 988 822 3020 490
Fragments arranged by size:
3020 1204 988 822 490
BsaAI yAC'GTr
Cuts at: 2721 3877 2721
Size: 1156 5368
BsaBI GATnn'nnATC
Cuts at: 6 2923 4038 6075 6
Size: 2917 1115 2037 455
Fragments arranged by size:
2917 2037 1115 455
BsaHI Gr'CG_yC
Cuts at: 443 554 637 3955 4203 4905 6401 443
Size: 111 83 3318 248 702 1496 566
Fragments arranged by size:
3318 1496 702 566 248 111 83
BsaWI w'CCGG_w
Cuts at: 571 782 3200 3713 4234 4912 5186 5333 5744
Size: 211 2418 513 521 678 274 147 411
Cuts at: 5744 571
Size: 1351
Fragments arranged by size:
2418 1351 678 521 513 411 274 211
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 277 307 2860 2890 3133 3163 277
Size: 30 2553 30 243 30 3638
Fragments arranged by size:
3638 2553 243 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 844 847 1093 3016 295
Size: 549 3 246 1923 3803
Fragments arranged by size:
3803 1923 549 246 3
BseYI C'CCAG_C
Cuts at: 280 324 712 727 885 901 1010 1018 1220
Size: 44 388 15 158 16 109 8 202
Cuts at: 1220 1383 1652 2143 2622 3949 4707 5284 5780
Size: 163 269 491 479 1327 758 577 496
Cuts at: 5780 280
Size: 1024
Fragments arranged by size:
1327 1024 758 577 496 491 479 388
269 202 163 158 109 44 16 15
8
BsiEI CG_ry'CG
Cuts at: 104 4066 4112 5320 104
Size: 3962 46 1208 1308
Fragments arranged by size:
3962 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 305 520 943 2720 3538 4316 4506 5298 305
Size: 215 423 1777 818 778 190 792 1531
Fragments arranged by size:
1777 1531 818 792 778 423 215 190
BsmI GAATG_Cn'
Cuts at: 1943 1943
Size: 6524
BsmBI CGTCTCn'nnnn_
Cuts at: 434 434
Size: 6524
Bsp1286I G_dGCh'C
Cuts at: 305 520 679 902 943 1007 1072 1156 1938
Size: 215 159 223 41 64 65 84 782
Cuts at: 1938 2477 2720 3227 3538 4149 4242 4316 4506
Size: 539 243 507 311 611 93 74 190
Cuts at: 4506 4568 5298 305
Size: 62 730 1531
Fragments arranged by size:
1531 782 730 611 539 507 311 243
223 215 190 159 93 84 74 65
64 62 41
BspEI T'CCGG_A
Cuts at: 782 5744 782
Size: 4962 1562
BspHI T'CATG_A
Cuts at: 2594 5700 2594
Size: 3106 3418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 263 1362 2752 3574 3788 4090 4471 176
Size: 87 1099 1390 822 214 302 381 2229
Fragments arranged by size:
2229 1390 1099 822 381 302 214 87
BsrBI CCG'CTC
Cuts at: 50 246 2187 4816 4870 6354 6519 50
Size: 196 1941 2629 54 1484 165 55
Fragments arranged by size:
2629 1941 1484 196 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2679 4436 2679
Size: 1757 4767
BsrFI r'CCGG_y
Cuts at: 314 571 869 1083 1799 2558 4522 4703 4912
Size: 257 298 214 716 759 1964 181 209
Cuts at: 4912 314
Size: 1926
Fragments arranged by size:
1964 1926 759 716 298 257 214 209
181
BssHII G'CGCG_C
Cuts at: 2136 2136
Size: 6524
BssSI C'ACGA_G
Cuts at: 4795 5153 4795
Size: 358 6166
BstAPI GCAn_nnn'nTGC
Cuts at: 3901 3901
Size: 6524
BstBI TT'CG_AA
Cuts at: 4885 4885
Size: 6524
BstEII G'GTnAC_C
Cuts at: 1426 1426
Size: 6524
BstXI CCAn_nnnn'nTGG
Cuts at: 151 151
Size: 6524
BstYI r'GATC_y
Cuts at: 1 351 959 1540 1587 2567 3025 3549 4016
Size: 350 608 581 47 980 458 524 467
Cuts at: 4016 4039 4374 4620 5632 5718 5730 6076 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 980 608 581 524 467 458 449
350 346 335 246 86 47 23 12
Bsu36I CC'TnA_GG
Cuts at: 913 1703 913
Size: 790 5734
BtgI C'CryG_G
Cuts at: 144 2699 144
Size: 2555 3969
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2219 4474 4655 4818 2219
Size: 2255 181 163 3925
Fragments arranged by size:
3925 2255 181 163
BtsI GCAGTG_nn'
Cuts at: 953 1182 1776 2394 2782 3767 953
Size: 229 594 618 388 985 3710
Fragments arranged by size:
3710 985 618 594 388 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1352 1387 1352
Size: 35 6489
DraI TTT'AAA
Cuts at: 3523 3523
Size: 6524
DraIII CAC_nnn'GTG
Cuts at: 2853 3250 2853
Size: 397 6127
DrdI GACnn_nn'nnGTC
Cuts at: 4230 5088 4230
Size: 858 5666
EaeI y'GGCC_r
Cuts at: 695 4109 4283 4674 4701 6046 695
Size: 3414 174 391 27 1345 1173
Fragments arranged by size:
3414 1345 1173 391 174 27
EagI C'GGCC_G
Cuts at: 4109 4109
Size: 6524
EarI CTCTTCn'nnn_
Cuts at: 1231 1503 1629 1786 1993 4547 4757 1231
Size: 272 126 157 207 2554 210 2998
Fragments arranged by size:
2998 2554 272 210 207 157 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3304 4732 5042 5188 6334 3304
Size: 1428 310 146 1146 3494
Fragments arranged by size:
3494 1428 1146 310 146
EcoICRI GAG'CTC
Cuts at: 941 3536 941
Size: 2595 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 273 895 1003 1465 1863 2152 2179 2185
Size: 79 622 108 462 398 289 27 6
Cuts at: 2185 2194 2734 2812 2820 2949 3004 3115 3127
Size: 9 540 78 8 129 55 111 12
Cuts at: 3127 4349 4781 5528 5796 194
Size: 1222 432 747 268 922
Fragments arranged by size:
1222 922 747 622 540 462 432 398
289 268 129 111 108 79 78 55
27 12 9 8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 912 2545 912
Size: 1633 4891
EcoO109I rG'GnC_Cy
Cuts at: 777 797 978 1196 2473 2474 2538 3266 3399
Size: 20 181 218 1277 1 64 728 133
Cuts at: 3399 3663 777
Size: 264 3638
Fragments arranged by size:
3638 1277 728 264 218 181 133 64
20 1
EcoRI G'AATT_C
Cuts at: 129 3528 129
Size: 3399 3125
EcoRV GAT'ATC
Cuts at: 611 611
Size: 6524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1302 4543 1302
Size: 3241 3283
FspI TGC'GCA
Cuts at: 1768 4305 1768
Size: 2537 3987
HaeII r_GCGC'y
Cuts at: 847 908 1109 2109 2454 2559 2799 3010 3958
Size: 61 201 1000 345 105 240 211 948
Cuts at: 3958 4206 5228 6207 847
Size: 248 1022 979 1164
Fragments arranged by size:
1164 1022 1000 979 948 345 248 240
211 201 105 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 595 627 1456 1488 1683 1715 1906 1938 3003
Size: 32 829 32 195 32 191 32 1065
Cuts at: 3003 3035 3133 3165 4366 4396 4398 4428 595
Size: 32 98 32 1201 30 2 30 2691
Fragments arranged by size:
2691 1201 1065 829 195 191 98 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3563 3563
Size: 6524
HindIII A'AGCT_T
Cuts at: 1951 1951
Size: 6524
KasI G'GCGC_C
Cuts at: 3954 4202 3954
Size: 248 6276
KpnI G_GTAC'C
Cuts at: 279 578 3544 279
Size: 299 2966 3259
Fragments arranged by size:
3259 2966 299
MluI A'CGCG_T
Cuts at: 5957 5957
Size: 6524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1104 3066 3167 3176 5195 5379 1104
Size: 1962 101 9 2019 184 2249
Fragments arranged by size:
2249 2019 1962 184 101 9
MscI TGG'CCA
Cuts at: 4285 4285
Size: 6524
MslI CAynn'nnrTG
Cuts at: 112 1829 2644 2809 4513 4640 112
Size: 1717 815 165 1704 127 1996
Fragments arranged by size:
1996 1717 1704 815 165 127
MspA1I CmG'CkG
Cuts at: 541 1025 1224 1255 1857 2575 2840 3635 3949
Size: 484 199 31 602 718 265 795 314
Cuts at: 3949 4309 5322 5567 541
Size: 360 1013 245 1498
Fragments arranged by size:
1498 1013 795 718 602 484 360 314
265 245 199 31
NaeI GCC'GGC
Cuts at: 1085 2560 4705 1085
Size: 1475 2145 2904
Fragments arranged by size:
2904 2145 1475
NarI GG'CG_CC
Cuts at: 3955 4203 3955
Size: 248 6276
NcoI C'CATG_G
Cuts at: 2699 2699
Size: 6524
NdeI CA'TA_TG
Cuts at: 1608 1608
Size: 6524
NgoMIV G'CCGG_C
Cuts at: 1083 2558 4703 1083
Size: 1475 2145 2904
Fragments arranged by size:
2904 2145 1475
NruI TCG'CGA
Cuts at: 2238 2238
Size: 6524
NsiI A_TGCA'T
Cuts at: 1473 1473
Size: 6524
NspI r_CATG'y
Cuts at: 657 1422 1842 3577 4077 4984 657
Size: 765 420 1735 500 907 2197
Fragments arranged by size:
2197 1735 907 765 500 420
PasI CC'CwG_GG
Cuts at: 2629 2629
Size: 6524
PciI A'CATG_T
Cuts at: 4980 4980
Size: 6524
PflMI CCAn_nnn'nTGG
Cuts at: 541 591 2363 2668 2853 541
Size: 50 1772 305 185 4212
Fragments arranged by size:
4212 1772 305 185 50
PfoI T'CCnGG_A
Cuts at: 599 854 599
Size: 255 6269
PmeI GTTT'AAAC
Cuts at: 3523 3523
Size: 6524
PmlI CAC'GTG
Cuts at: 2721 2721
Size: 6524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5663 5695 5663
Size: 32 6492
PpuMI rG'GwC_Cy
Cuts at: 797 978 1196 2538 797
Size: 181 218 1342 4783
Fragments arranged by size:
4783 1342 218 181
PspOMI G'GGCC_C
Cuts at: 1152 1934 2473 1152
Size: 782 539 5203
Fragments arranged by size:
5203 782 539
PstI C_TGCA'G
Cuts at: 274 751 2449 3571 4256 274
Size: 477 1698 1122 685 2542
Fragments arranged by size:
2542 1698 1122 685 477
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6524
PvuII CAG'CTG
Cuts at: 541 1025 1857 2840 3949 4309 541
Size: 484 832 983 1109 360 2756
Fragments arranged by size:
2756 1109 983 832 484 360
RsrII CG'GwC_CG
Cuts at: 4719 4719
Size: 6524
SacI G_AGCT'C
Cuts at: 943 3538 943
Size: 2595 3929
SalI G'TCGA_C
Cuts at: 3561 3561
Size: 6524
SanDI GG'GwC_CC
Cuts at: 1196 1196
Size: 6524
SapI GCTCTTCn'nnn_
Cuts at: 1629 1786 4547 4757 1629
Size: 157 2761 210 3396
Fragments arranged by size:
3396 2761 210 157
SbfI CC_TGCA'GG
Cuts at: 274 3571 274
Size: 3297 3227
ScaI AGT'ACT
Cuts at: 2203 3427 6041 2203
Size: 1224 2614 2686
Fragments arranged by size:
2686 2614 1224
SfcI C'TryA_G
Cuts at: 33 270 747 1029 2445 3567 4252 5245 5436
Size: 237 477 282 1416 1122 685 993 191
Cuts at: 5436 33
Size: 1121
Fragments arranged by size:
1416 1122 1121 993 685 477 282 237
191
SfoI GGC'GCC
Cuts at: 3956 4204 3956
Size: 248 6276
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6524
SgrAI Cr'CCGG_yG
Cuts at: 2558 2558
Size: 6524
SmaI CCC'GGG
Cuts at: 298 530 550 1194 3546 298
Size: 232 20 644 2352 3276
Fragments arranged by size:
3276 2352 644 232 20
SmlI C'TyrA_G
Cuts at: 307 347 2221 2706 3325 3679 4594 5086 5348
Size: 40 1874 485 619 354 915 492 262
Cuts at: 5348 307
Size: 1483
Fragments arranged by size:
1874 1483 915 619 492 485 354 262
40
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6524
SphI G_CATG'C
Cuts at: 3577 4077 3577
Size: 500 6024
StuI AGG'CCT
Cuts at: 3283 3283
Size: 6524
StyI C'CwwG_G
Cuts at: 226 462 1064 1073 1595 1673 2357 2511 2534
Size: 236 602 9 522 78 684 154 23
Cuts at: 2534 2699 3073 3658 4012 226
Size: 165 374 585 354 2738
Fragments arranged by size:
2738 684 602 585 522 374 354 236
165 154 78 23 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 390 1362 2300 3047 4107 4240 4699 4908
Size: 233 972 938 747 1060 133 459 209
Cuts at: 4908 157
Size: 1773
Fragments arranged by size:
1773 1060 972 938 747 459 233 209
133
TatI w'GTAC_w
Cuts at: 1715 2201 2492 3425 6039 1715
Size: 486 291 933 2614 2200
Fragments arranged by size:
2614 2200 933 486 291
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 1420 1452 2716 2748 2880 2912 6041
Size: 32 1111 32 1264 32 132 32 3129
Cuts at: 6041 6073 277
Size: 32 728
Fragments arranged by size:
3129 1264 1111 728 132 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 4321 2926
Size: 1395 5129
XbaI T'CTAG_A
Cuts at: 3555 3555
Size: 6524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 151 1534 2946 4019 151
Size: 1383 1412 1073 2656
Fragments arranged by size:
2656 1412 1383 1073
XmaI C'CCGG_G
Cuts at: 296 528 548 1192 3544 296
Size: 232 20 644 2352 3276
Fragments arranged by size:
3276 2352 644 232 20
XmnI GAAnn'nnTTC
Cuts at: 423 2653 423
Size: 2230 4294
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BcgI BmgBI BmtI BsgI BsiWI BsrGI BstZ17I ClaI
FseI FspAI HpaI MfeI NheI NotI PacI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SnaBI SrfI
SspI SwaI XhoI ZraI