Restriction map for
pF1KB4815
(Circular) (Six-base) MAPSORT of: pF1KB4815.seq Check: 6954 from: 1 to: 7535
pF1KB4815 7535 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1200 1868 2344 1200
Size: 668 476 6391
Fragments arranged by size:
6391 668 476
AccI GT'mk_AC
Cuts at: 602 2214 4073 4573 602
Size: 1612 1859 500 3564
Fragments arranged by size:
3564 1859 1612 500
Acc65I G'GTAC_C
Cuts at: 1354 4551 1354
Size: 3197 4338
AclI AA'CG_TT
Cuts at: 7375 7375
Size: 7535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1597 1806 2228 2447 2756 2849 2912 3265
Size: 537 209 422 219 309 93 63 353
Cuts at: 3265 3938 4303 5360 5792 6539 6807 1060
Size: 673 365 1057 432 747 268 1788
Fragments arranged by size:
1788 1057 747 673 537 432 422 365
353 309 268 219 209 93 63
AflIII A'CryG_T
Cuts at: 894 2217 4887 5991 6968 894
Size: 1323 2670 1104 977 1461
Fragments arranged by size:
2670 1461 1323 1104 977
AgeI A'CCGG_T
Cuts at: 5923 5923
Size: 7535
AleI CACnn'nnGTG
Cuts at: 3871 3871
Size: 7535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3851 3851
Size: 7535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 394 426 394
Size: 32 7503
AlwNI CAG_nnn'CTG
Cuts at: 184 283 2383 2779 4149 4386 4840 6407 184
Size: 99 2100 396 1370 237 454 1567 1312
Fragments arranged by size:
2100 1567 1370 1312 454 396 237 99
ApaLI G'TGCA_C
Cuts at: 6305 6305
Size: 7535
ApoI r'AATT_y
Cuts at: 445 541 775 973 1193 2712 3973 4539 6888
Size: 96 234 198 220 1519 1261 566 2349
Cuts at: 6888 7144 445
Size: 256 836
Fragments arranged by size:
2349 1519 1261 836 566 256 234 220
198 96
AseI AT'TA_AT
Cuts at: 20 2057 20
Size: 2037 5498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7535
AvaI C'yCGr_G
Cuts at: 2939 3263 4555 2939
Size: 324 1292 5919
Fragments arranged by size:
5919 1292 324
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2063 2096 2063
Size: 33 7502
BamHI G'GATC_C
Cuts at: 612 4560 612
Size: 3948 3587
BanI G'GyrC_C
Cuts at: 1354 1561 2766 3678 4551 4965 5213 5248 1354
Size: 207 1205 912 873 414 248 35 3641
Fragments arranged by size:
3641 1205 912 873 414 248 207 35
BanII G_rGCy'C
Cuts at: 701 712 1496 1946 2495 3355 4549 5579 701
Size: 11 784 450 549 860 1194 1030 2657
Fragments arranged by size:
2657 1194 1030 860 784 549 450 11
BbeI G_GCGC'C
Cuts at: 4969 5217 4969
Size: 248 7287
BbsI GAAGACnn'nnnn_
Cuts at: 380 1023 1667 2033 3253 3511 3944 380
Size: 643 644 366 1220 258 433 3971
Fragments arranged by size:
3971 1220 644 643 433 366 258
BbvCI CC'TCA_GC
Cuts at: 2363 3719 2363
Size: 1356 6179
BciVI GTATCCnnnnn_n'
Cuts at: 5433 6194 7535 5433
Size: 761 1341 5433
Fragments arranged by size:
5433 1341 761
BclI T'GATC_A
Cuts at: 5055 5055
Size: 7535
BfrBI ATG'CAT
Cuts at: 2199 2570 2199
Size: 371 7164
BglII A'GATC_T
Cuts at: 4309 4428 4515 5050 7087 4309
Size: 119 87 535 2037 4757
Fragments arranged by size:
4757 2037 535 119 87
BlpI GC'TnA_GC
Cuts at: 1940 4647 1940
Size: 2707 4828
Bme1580I G_kGCm'C
Cuts at: 5160 5253 6309 5160
Size: 93 1056 6386
Fragments arranged by size:
6386 1056 93
BmgBI CAC'GTC
Cuts at: 1758 1758
Size: 7535
BmrI ACTGGGnnnn_n'
Cuts at: 1640 2614 3932 4414 4747 4828 5163 7307 1640
Size: 974 1318 482 333 81 335 2144 1868
Fragments arranged by size:
2144 1868 1318 974 482 335 333 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5902 5902
Size: 7535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 2060 2989 3083 3457 3511 4063 358
Size: 1702 929 94 374 54 552 3830
Fragments arranged by size:
3830 1702 929 552 374 94 54
Bpu10I CC'TnA_GC
Cuts at: 2363 3719 2363
Size: 1356 6179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 1510 2521 2729 3592 4711 5590 6082 6380
Size: 858 1011 208 863 1119 879 492 298
Cuts at: 6380 652
Size: 1807
Fragments arranged by size:
1807 1119 1011 879 863 858 492 298
208
BsaI GGTCTCn'nnnn_
Cuts at: 226 2287 4020 4269 7239 226
Size: 2061 1733 249 2970 522
Fragments arranged by size:
2970 2061 1733 522 249
BsaAI yAC'GTr
Cuts at: 2218 4888 2218
Size: 2670 4865
BsaBI GATnn'nnATC
Cuts at: 6 5049 7086 6
Size: 5043 2037 455
Fragments arranged by size:
5043 2037 455
BsaHI Gr'CG_yC
Cuts at: 173 1235 1951 4966 5214 5916 7412 173
Size: 1062 716 3015 248 702 1496 296
Fragments arranged by size:
3015 1496 1062 716 702 296 248
BsaWI w'CCGG_w
Cuts at: 4724 5245 5923 6197 6344 6755 4724
Size: 521 678 274 147 411 5504
Fragments arranged by size:
5504 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 394 424 1273 1303 1607 1637 1823 1853 1860
Size: 30 849 30 304 30 186 30 7
Cuts at: 1860 1890 2058 2088 2363 2393 394
Size: 30 168 30 275 30 5536
Fragments arranged by size:
5536 849 304 275 186 168 30 30
30 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 415 1868 3401 3736 3843 139
Size: 276 1453 1533 335 107 3831
Fragments arranged by size:
3831 1533 1453 335 276 107
BseYI C'CCAG_C
Cuts at: 1569 1842 3764 4960 5718 6295 6791 1569
Size: 273 1922 1196 758 577 496 2313
Fragments arranged by size:
2313 1922 1196 758 577 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2605 3917 4162 2605
Size: 1312 245 5978
Fragments arranged by size:
5978 1312 245
BsiEI CG_ry'CG
Cuts at: 104 4247 5077 5123 6331 104
Size: 4143 830 46 1208 1308
Fragments arranged by size:
4143 1308 1208 830 46
BsiHKAI G_wGCw'C
Cuts at: 758 1946 2495 3355 3746 4549 5327 5517 6309
Size: 1188 549 860 391 803 778 190 792
Cuts at: 6309 758
Size: 1984
Fragments arranged by size:
1984 1188 860 803 792 778 549 391
190
BsiWI C'GTAC_G
Cuts at: 2298 2298
Size: 7535
BsmI GAATG_Cn'
Cuts at: 2137 3840 2137
Size: 1703 5832
BsmBI CGTCTCn'nnnn_
Cuts at: 4462 4462
Size: 7535
Bsp1286I G_dGCh'C
Cuts at: 701 712 758 1496 1946 2495 3355 3746 4549
Size: 11 46 738 450 549 860 391 803
Cuts at: 4549 5160 5253 5327 5517 5579 6309 701
Size: 611 93 74 190 62 730 1927
Fragments arranged by size:
1927 860 803 738 730 611 549 450
391 190 93 74 62 46 11
BspEI T'CCGG_A
Cuts at: 6755 6755
Size: 7535
BspHI T'CATG_A
Cuts at: 1503 1925 6711 1503
Size: 422 4786 2327
Fragments arranged by size:
4786 2327 422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1200 1868 1976 2344 3568 4002 4585 4799 5101
Size: 668 108 368 1224 434 583 214 302
Cuts at: 5101 5482 1200
Size: 381 3253
Fragments arranged by size:
3253 1224 668 583 434 381 368 302
214 108
BsrBI CCG'CTC
Cuts at: 50 4226 5827 5881 7365 7530 50
Size: 4176 1601 54 1484 165 55
Fragments arranged by size:
4176 1601 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1706 3480 4018 5447 1706
Size: 1774 538 1429 3794
Fragments arranged by size:
3794 1774 1429 538
BsrFI r'CCGG_y
Cuts at: 5533 5714 5923 5533
Size: 181 209 7145
Fragments arranged by size:
7145 209 181
BsrGI T'GTAC_A
Cuts at: 1320 2105 1320
Size: 785 6750
BssSI C'ACGA_G
Cuts at: 5806 6164 5806
Size: 358 7177
BstAPI GCAn_nnn'nTGC
Cuts at: 4149 4912 4149
Size: 763 6772
BstBI TT'CG_AA
Cuts at: 5896 5896
Size: 7535
BstEII G'GTnAC_C
Cuts at: 3139 3580 3139
Size: 441 7094
BstXI CCAn_nnnn'nTGG
Cuts at: 468 1350 1809 468
Size: 882 459 6194
Fragments arranged by size:
6194 882 459
BstYI r'GATC_y
Cuts at: 1 612 1082 2324 4309 4428 4515 4560 5027
Size: 611 470 1242 1985 119 87 45 467
Cuts at: 5027 5050 5385 5631 6643 6729 6741 7087 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1985 1242 1012 611 470 467 449 346
335 246 119 87 86 45 23 12
BstZ17I GTA'TAC
Cuts at: 603 603
Size: 7535
BtgI C'CryG_G
Cuts at: 107 557 767 942 996 1301 2543 3525 107
Size: 450 210 175 54 305 1242 982 4117
Fragments arranged by size:
4117 1242 982 450 305 210 175 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 615 3411 5485 5666 5829 615
Size: 2796 2074 181 163 2321
Fragments arranged by size:
2796 2321 2074 181 163
BtsI GCAGTG_nn'
Cuts at: 1445 2453 2643 3361 3550 4778 1445
Size: 1008 190 718 189 1228 4202
Fragments arranged by size:
4202 1228 1008 718 190 189
ClaI AT'CG_AT
Cuts at: 3281 3281
Size: 7535
DraI TTT'AAA
Cuts at: 4534 4534
Size: 7535
DraIII CAC_nnn'GTG
Cuts at: 2399 2399
Size: 7535
DrdI GACnn_nn'nnGTC
Cuts at: 178 490 2210 3646 3948 5241 6099 178
Size: 312 1720 1436 302 1293 858 1614
Fragments arranged by size:
1720 1614 1436 1293 858 312 302
EaeI y'GGCC_r
Cuts at: 1518 1799 5120 5294 5685 5712 7057 1518
Size: 281 3321 174 391 27 1345 1996
Fragments arranged by size:
3321 1996 1345 391 281 174 27
EagI C'GGCC_G
Cuts at: 5120 5120
Size: 7535
EarI CTCTTCn'nnn_
Cuts at: 331 833 1036 1427 2244 2273 2454 4112 5558
Size: 502 203 391 817 29 181 1658 1446
Cuts at: 5558 5768 331
Size: 210 2098
Fragments arranged by size:
2098 1658 1446 817 502 391 210 203
181 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 3402 5743 6053 6199 7345 358
Size: 3044 2341 310 146 1146 548
Fragments arranged by size:
3044 2341 1146 548 310 146
EcoICRI GAG'CTC
Cuts at: 1944 2493 3353 4547 1944
Size: 549 860 1194 4932
Fragments arranged by size:
4932 1194 860 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1060 1597 1806 2060 2228 2447 2756 2849
Size: 702 537 209 254 168 219 309 93
Cuts at: 2849 2912 2989 3083 3265 3457 3511 3938 4063
Size: 63 77 94 182 192 54 427 125
Cuts at: 4063 4303 5360 5792 6539 6807 358
Size: 240 1057 432 747 268 1086
Fragments arranged by size:
1086 1057 747 702 537 432 427 309
268 254 240 219 209 192 182 168
125 94 93 77 63 54
EcoNI CCTnn'n_nnAGG
Cuts at: 4366 4366
Size: 7535
EcoO109I rG'GnC_Cy
Cuts at: 1983 2411 3786 4674 1983
Size: 428 1375 888 4844
Fragments arranged by size:
4844 1375 888 428
EcoRI G'AATT_C
Cuts at: 1193 4539 1193
Size: 3346 4189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1605 5554 1605
Size: 3949 3586
FspI TGC'GCA
Cuts at: 5316 5316
Size: 7535
HaeII r_GCGC'y
Cuts at: 3663 4969 5217 6239 7218 3663
Size: 1306 248 1022 979 3980
Fragments arranged by size:
3980 1306 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1483 1515 1607 1639 2363 2395 2429 2461 2933
Size: 32 92 32 724 32 34 32 472
Cuts at: 2933 2965 3094 3126 3257 3289 3718 3750 3778
Size: 32 129 32 131 32 429 32 28
Cuts at: 3778 3810 4137 4169 4357 4389 5377 5407 5409
Size: 32 327 32 188 32 988 30 2
Cuts at: 5409 5439 1483
Size: 30 3579
Fragments arranged by size:
3579 988 724 472 429 327 188 131
129 92 34 32 32 32 32 32
32 32 32 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 4574 4574
Size: 7535
KasI G'GCGC_C
Cuts at: 4965 5213 4965
Size: 248 7287
KpnI G_GTAC'C
Cuts at: 1358 4555 1358
Size: 3197 4338
MfeI C'AATT_G
Cuts at: 4484 4484
Size: 7535
MluI A'CGCG_T
Cuts at: 6968 6968
Size: 7535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 959 4259 6206 6390 959
Size: 3300 1947 184 2104
Fragments arranged by size:
3300 2104 1947 184
MscI TGG'CCA
Cuts at: 1520 1801 5296 1520
Size: 281 3495 3759
Fragments arranged by size:
3759 3495 281
MslI CAynn'nnrTG
Cuts at: 466 3170 3871 4051 5524 5651 466
Size: 2704 701 180 1473 127 2350
Fragments arranged by size:
2704 2350 1473 701 180 127
MspA1I CmG'CkG
Cuts at: 1038 1873 2589 4646 4960 5320 6333 6578 1038
Size: 835 716 2057 314 360 1013 245 1995
Fragments arranged by size:
2057 1995 1013 835 716 360 314 245
NaeI GCC'GGC
Cuts at: 5716 5716
Size: 7535
NarI GG'CG_CC
Cuts at: 4966 5214 4966
Size: 248 7287
NcoI C'CATG_G
Cuts at: 107 557 996 3525 107
Size: 450 439 2529 4117
Fragments arranged by size:
4117 2529 450 439
NdeI CA'TA_TG
Cuts at: 873 3310 873
Size: 2437 5098
NgoMIV G'CCGG_C
Cuts at: 5714 5714
Size: 7535
NsiI A_TGCA'T
Cuts at: 2201 2572 2201
Size: 371 7164
NspI r_CATG'y
Cuts at: 898 2275 4588 5088 5995 898
Size: 1377 2313 500 907 2438
Fragments arranged by size:
2438 2313 1377 907 500
PasI CC'CwG_GG
Cuts at: 1999 3840 1999
Size: 1841 5694
PciI A'CATG_T
Cuts at: 894 5991 894
Size: 5097 2438
PflMI CCAn_nnn'nTGG
Cuts at: 2878 2878
Size: 7535
PmeI GTTT'AAAC
Cuts at: 4534 4534
Size: 7535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 394 426 1620 1652 6674 6706 394
Size: 32 1194 32 5022 32 1223
Fragments arranged by size:
5022 1223 1194 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1983 3786 1983
Size: 1803 5732
PshAI GACnn'nnGTC
Cuts at: 4333 4333
Size: 7535
PspXI vC'TCGA_Gb
Cuts at: 3263 3263
Size: 7535
PstI C_TGCA'G
Cuts at: 4010 4582 5267 4010
Size: 572 685 6278
Fragments arranged by size:
6278 685 572
PvuI CG_AT'CG
Cuts at: 104 4247 104
Size: 4143 3392
PvuII CAG'CTG
Cuts at: 1038 2589 4960 5320 1038
Size: 1551 2371 360 3253
Fragments arranged by size:
3253 2371 1551 360
RsrII CG'GwC_CG
Cuts at: 5730 5730
Size: 7535
SacI G_AGCT'C
Cuts at: 1946 2495 3355 4549 1946
Size: 549 860 1194 4932
Fragments arranged by size:
4932 1194 860 549
SalI G'TCGA_C
Cuts at: 4572 4572
Size: 7535
SapI GCTCTTCn'nnn_
Cuts at: 2273 5558 5768 2273
Size: 3285 210 4040
Fragments arranged by size:
4040 3285 210
SbfI CC_TGCA'GG
Cuts at: 4582 4582
Size: 7535
ScaI AGT'ACT
Cuts at: 2308 3605 7052 2308
Size: 1297 3447 2791
Fragments arranged by size:
3447 2791 1297
SfcI C'TryA_G
Cuts at: 33 3180 4006 4578 5263 6256 6447 33
Size: 3147 826 572 685 993 191 1121
Fragments arranged by size:
3147 1121 993 826 685 572 191
SfoI GGC'GCC
Cuts at: 4967 5215 4967
Size: 248 7287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7535
SmaI CCC'GGG
Cuts at: 4557 4557
Size: 7535
SmlI C'TyrA_G
Cuts at: 667 1489 2536 2744 3263 3607 4690 5605 6097
Size: 822 1047 208 519 344 1083 915 492
Cuts at: 6097 6359 667
Size: 262 1843
Fragments arranged by size:
1843 1083 1047 915 822 519 492 344
262 208
SpeI A'CTAG_T
Cuts at: 67 597 67
Size: 530 7005
SphI G_CATG'C
Cuts at: 4588 5088 4588
Size: 500 7035
SspI AAT'ATT
Cuts at: 4101 4101
Size: 7535
StuI AGG'CCT
Cuts at: 2527 2527
Size: 7535
StyI C'CwwG_G
Cuts at: 107 296 557 996 1025 3113 3525 3674 4669
Size: 189 261 439 29 2088 412 149 995
Cuts at: 4669 5023 107
Size: 354 2619
Fragments arranged by size:
2619 2088 995 439 412 354 261 189
149 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1404 3874 5118 5251 5710 5919 1404
Size: 2470 1244 133 459 209 3020
Fragments arranged by size:
3020 2470 1244 459 209 133
TatI w'GTAC_w
Cuts at: 798 1320 2086 2105 2306 3063 3603 4493 7050
Size: 522 766 19 201 757 540 890 2557
Cuts at: 7050 798
Size: 1283
Fragments arranged by size:
2557 1283 890 766 757 540 522 201
19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1088 1120 1634 1666 1823 1855 1860 1892 2058
Size: 32 514 32 157 32 5 32 166
Cuts at: 2058 2090 7052 7084 1088
Size: 32 4962 32 1539
Fragments arranged by size:
4962 1539 514 166 157 32 32 32
32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 2800 5332 2800
Size: 2532 5003
XbaI T'CTAG_A
Cuts at: 4566 4566
Size: 7535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 658 1347 3013 3432 3552 3681 5030 658
Size: 689 1666 419 120 129 1349 3163
Fragments arranged by size:
3163 1666 1349 689 419 129 120
XhoI C'TCGA_G
Cuts at: 3263 3263
Size: 7535
XmaI C'CCGG_G
Cuts at: 4555 4555
Size: 7535
XmnI GAAnn'nnTTC
Cuts at: 569 779 569
Size: 210 7325
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BcgI
BglI BmtI BssHII Bsu36I CspCI EcoRV FseI FspAI
HindIII HpaI NheI NotI NruI PacI PfoI PmlI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI ZraI