Restriction map for
pF1KB4822
(Circular) (Six-base) MAPSORT of: pF1KB4822.seq Check: 7100 from: 1 to: 4466
pF1KB4822 4466 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1504 1504
Size: 4466
Acc65I G'GTAC_C
Cuts at: 1482 1482
Size: 4466
AclI AA'CG_TT
Cuts at: 4306 4306
Size: 4466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2291 2723 3076 3738 2291
Size: 432 353 662 3019
Fragments arranged by size:
3019 662 432 353
AflIII A'CryG_T
Cuts at: 1818 3618 3899 1818
Size: 1800 281 2385
Fragments arranged by size:
2385 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1235 1235
Size: 4466
AlwNI CAG_nnn'CTG
Cuts at: 189 978 1771 3209 189
Size: 789 793 1438 1446
Fragments arranged by size:
1446 1438 793 789
ApaI G_GGCC'C
Cuts at: 1124 1124
Size: 4466
ApaLI G'TGCA_C
Cuts at: 3304 3304
Size: 4466
ApoI r'AATT_y
Cuts at: 1470 3819 4075 1470
Size: 2349 256 1861
Fragments arranged by size:
2349 1861 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4466
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
AvaI C'yCGr_G
Cuts at: 220 919 1486 220
Size: 699 567 3200
Fragments arranged by size:
3200 699 567
BamHI G'GATC_C
Cuts at: 1491 1491
Size: 4466
BanI G'GyrC_C
Cuts at: 1482 1896 2144 2179 1482
Size: 414 248 35 3769
Fragments arranged by size:
3769 414 248 35
BanII G_rGCy'C
Cuts at: 1079 1124 1480 2510 1079
Size: 45 356 1030 3035
Fragments arranged by size:
3035 1030 356 45
BbeI G_GCGC'C
Cuts at: 1900 2148 1900
Size: 248 4218
BbsI GAAGACnn'nnnn_
Cuts at: 394 394
Size: 4466
BciVI GTATCCnnnnn_n'
Cuts at: 2364 3420 4466 2364
Size: 1056 1046 2364
Fragments arranged by size:
2364 1056 1046
BclI T'GATC_A
Cuts at: 1986 1986
Size: 4466
BglI GCCn_nnn'nGGC
Cuts at: 923 923
Size: 4466
BglII A'GATC_T
Cuts at: 1432 1981 4018 1432
Size: 549 2037 1880
Fragments arranged by size:
2037 1880 549
BlpI GC'TnA_GC
Cuts at: 1030 1578 1030
Size: 548 3918
Bme1580I G_kGCm'C
Cuts at: 333 780 1124 2091 2184 3308 333
Size: 447 344 967 93 1124 1491
Fragments arranged by size:
1491 1124 967 447 344 93
BmgBI CAC'GTC
Cuts at: 911 911
Size: 4466
BmrI ACTGGGnnnn_n'
Cuts at: 1122 1678 1759 2094 4238 1122
Size: 556 81 335 2144 1350
Fragments arranged by size:
2144 1350 556 335 81
BmtI G_CTAG'C
Cuts at: 1336 1336
Size: 4466
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 550 660 1060 1177 136
Size: 414 110 400 117 3425
Fragments arranged by size:
3425 414 400 117 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1642 2521 3235 3533 1642
Size: 879 714 298 2575
Fragments arranged by size:
2575 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1157 4170 1157
Size: 3013 1453
BsaAI yAC'GTr
Cuts at: 950 1320 1819 950
Size: 370 499 3597
Fragments arranged by size:
3597 499 370
BsaBI GATnn'nnATC
Cuts at: 6 1980 4017 6
Size: 1974 2037 455
Fragments arranged by size:
2037 1974 455
BsaHI Gr'CG_yC
Cuts at: 308 956 1897 2145 2847 4343 308
Size: 648 941 248 702 1496 431
Fragments arranged by size:
1496 941 702 648 431 248
BsaWI w'CCGG_w
Cuts at: 1655 2176 2854 3265 3412 3686 1655
Size: 521 678 411 147 274 2435
Fragments arranged by size:
2435 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1219 1249 1219
Size: 30 4436
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 316 1288 256
Size: 60 972 3434
Fragments arranged by size:
3434 972 60
BseYI C'CCAG_C
Cuts at: 591 596 729 1891 2649 3314 3722 591
Size: 5 133 1162 758 665 408 1335
Fragments arranged by size:
1335 1162 758 665 408 133 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 847
Size: 4466
BsiEI CG_ry'CG
Cuts at: 104 129 633 1143 1233 2008 2054 3284 104
Size: 25 504 510 90 775 46 1230 1286
Fragments arranged by size:
1286 1230 775 510 504 90 46 25
BsiHKAI G_wGCw'C
Cuts at: 1480 2258 2448 3308 1480
Size: 778 190 860 2638
Fragments arranged by size:
2638 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 333 780 1079 1124 1480 2091 2184 2258 2448
Size: 447 299 45 356 611 93 74 190
Cuts at: 2448 2510 3308 333
Size: 62 798 1491
Fragments arranged by size:
1491 798 611 447 356 299 190 93
74 62 45
BspHI T'CATG_A
Cuts at: 2898 2898
Size: 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 1180 1516 1730 2032 2413 591
Size: 589 336 214 302 381 2644
Fragments arranged by size:
2644 589 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 444 1235 2758 2812 4296 4461 50
Size: 394 791 1523 54 1484 165 55
Fragments arranged by size:
1523 1484 791 394 165 55 54
BsrDI GCAATG_nn'
Cuts at: 426 2378 426
Size: 1952 2514
BsrFI r'CCGG_y
Cuts at: 857 1142 2464 2645 857
Size: 285 1322 181 2678
Fragments arranged by size:
2678 1322 285 181
BsrGI T'GTAC_A
Cuts at: 479 479
Size: 4466
BssSI C'ACGA_G
Cuts at: 892 2737 3445 892
Size: 1845 708 1913
Fragments arranged by size:
1913 1845 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1843 1843
Size: 4466
BstBI TT'CG_AA
Cuts at: 2827 2827
Size: 4466
BstXI CCAn_nnnn'nTGG
Cuts at: 114 966 1155 114
Size: 852 189 3425
Fragments arranged by size:
3425 852 189
BstYI r'GATC_y
Cuts at: 1 932 1106 1432 1491 1958 1981 2316 2562
Size: 931 174 326 59 467 23 335 246
Cuts at: 2562 2868 2880 2966 4018 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 931 467 449 335 326 306 246
174 86 59 23 12
Bsu36I CC'TnA_GG
Cuts at: 494 1381 494
Size: 887 3579
BtgI C'CryG_G
Cuts at: 708 939 1227 708
Size: 231 288 3947
Fragments arranged by size:
3947 288 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2416 2597 2760 2416
Size: 181 163 4122
Fragments arranged by size:
4122 181 163
BtsI GCAGTG_nn'
Cuts at: 1709 1709
Size: 4466
DraI TTT'AAA
Cuts at: 339 1465 339
Size: 1126 3340
DrdI GACnn_nn'nnGTC
Cuts at: 2172 3516 2172
Size: 1344 3122
EaeI y'GGCC_r
Cuts at: 126 711 1002 1230 2051 2225 2616 2643 3988
Size: 585 291 228 821 174 391 27 1345
Cuts at: 3988 126
Size: 604
Fragments arranged by size:
1345 821 604 585 391 291 228 174
27
EagI C'GGCC_G
Cuts at: 126 1230 2051 126
Size: 1104 821 2541
Fragments arranged by size:
2541 1104 821
EarI CTCTTCn'nnn_
Cuts at: 241 322 2489 2699 241
Size: 81 2167 210 2008
Fragments arranged by size:
2167 2008 210 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 149 169 1429 2674 3416 3562 4276 149
Size: 20 1260 1245 742 146 714 339
Fragments arranged by size:
1260 1245 742 714 339 146 20
EcoICRI GAG'CTC
Cuts at: 1478 1478
Size: 4466
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 550 660 1060 1177 2291 2723 3076 3738
Size: 414 110 400 117 1114 432 353 662
Cuts at: 3738 136
Size: 864
Fragments arranged by size:
1114 864 662 432 414 400 353 117
110
EcoNI CCTnn'n_nnAGG
Cuts at: 895 970 895
Size: 75 4391
EcoO109I rG'GnC_Cy
Cuts at: 534 975 1121 1605 534
Size: 441 146 484 3395
Fragments arranged by size:
3395 484 441 146
EcoRI G'AATT_C
Cuts at: 1470 1470
Size: 4466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 270 2485 270
Size: 2215 2251
FspI TGC'GCA
Cuts at: 2247 2247
Size: 4466
HaeII r_GCGC'y
Cuts at: 1132 1900 2148 3378 4149 1132
Size: 768 248 1230 771 1449
Fragments arranged by size:
1449 1230 771 768 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 344 376 2308 2338 2340 2370 344
Size: 32 1932 30 2 30 2440
Fragments arranged by size:
2440 1932 32 30 30 2
HincII GTy'rAC
Cuts at: 1505 1505
Size: 4466
KasI G'GCGC_C
Cuts at: 1896 2144 1896
Size: 248 4218
KpnI G_GTAC'C
Cuts at: 1486 1486
Size: 4466
MluI A'CGCG_T
Cuts at: 3899 3899
Size: 4466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3225 3409 3225
Size: 184 4282
MscI TGG'CCA
Cuts at: 1004 2227 1004
Size: 1223 3243
MslI CAynn'nnrTG
Cuts at: 950 2455 2582 950
Size: 1505 127 2834
Fragments arranged by size:
2834 1505 127
MspA1I CmG'CkG
Cuts at: 209 453 710 1577 1891 2251 3035 3280 209
Size: 244 257 867 314 360 784 245 1395
Fragments arranged by size:
1395 867 784 360 314 257 245 244
NaeI GCC'GGC
Cuts at: 859 2647 859
Size: 1788 2678
NarI GG'CG_CC
Cuts at: 1897 2145 1897
Size: 248 4218
NcoI C'CATG_G
Cuts at: 939 939
Size: 4466
NgoMIV G'CCGG_C
Cuts at: 857 2645 857
Size: 1788 2678
NheI G'CTAG_C
Cuts at: 1332 1332
Size: 4466
NspI r_CATG'y
Cuts at: 1519 2019 3622 1519
Size: 500 1603 2363
Fragments arranged by size:
2363 1603 500
PciI A'CATG_T
Cuts at: 3618 3618
Size: 4466
PfoI T'CCnGG_A
Cuts at: 653 798 653
Size: 145 4321
PmeI GTTT'AAAC
Cuts at: 1465 1465
Size: 4466
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 4434
PpuMI rG'GwC_Cy
Cuts at: 534 975 534
Size: 441 4025
PspOMI G'GGCC_C
Cuts at: 1120 1120
Size: 4466
PstI C_TGCA'G
Cuts at: 1513 2198 1513
Size: 685 3781
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4466
PvuII CAG'CTG
Cuts at: 453 1891 2251 453
Size: 1438 360 2668
Fragments arranged by size:
2668 1438 360
RsrII CG'GwC_CG
Cuts at: 2661 2661
Size: 4466
SacI G_AGCT'C
Cuts at: 1480 1480
Size: 4466
SacII CC_GC'GG
Cuts at: 711 711
Size: 4466
SalI G'TCGA_C
Cuts at: 1503 1503
Size: 4466
SapI GCTCTTCn'nnn_
Cuts at: 241 322 2489 2699 241
Size: 81 2167 210 2008
Fragments arranged by size:
2167 2008 210 81
SbfI CC_TGCA'GG
Cuts at: 1513 1513
Size: 4466
ScaI AGT'ACT
Cuts at: 3983 3983
Size: 4466
SexAI A'CCwGG_T
Cuts at: 971 971
Size: 4466
SfcI C'TryA_G
Cuts at: 33 1509 2194 3162 3353 33
Size: 1476 685 968 191 1146
Fragments arranged by size:
1476 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1898 2146 1898
Size: 248 4218
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4466
SmaI CCC'GGG
Cuts at: 222 1488 222
Size: 1266 3200
SmlI C'TyrA_G
Cuts at: 1621 2536 3250 3512 1621
Size: 915 714 262 2575
Fragments arranged by size:
2575 915 714 262
SnaBI TAC'GTA
Cuts at: 950 950
Size: 4466
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4466
SphI G_CATG'C
Cuts at: 1519 2019 1519
Size: 500 3966
StyI C'CwwG_G
Cuts at: 839 939 1600 1954 839
Size: 100 661 354 3351
Fragments arranged by size:
3351 661 354 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 647 2049 2182 2641 2850 647
Size: 1402 133 459 209 2263
Fragments arranged by size:
2263 1402 459 209 133
TatI w'GTAC_w
Cuts at: 479 875 3981 479
Size: 396 3106 964
Fragments arranged by size:
3106 964 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1219 1251 3983 4015 1219
Size: 32 2732 32 1670
Fragments arranged by size:
2732 1670 32 32
Tth111I GACn'n_nGTC
Cuts at: 649 909 973 2263 649
Size: 260 64 1290 2852
Fragments arranged by size:
2852 1290 260 64
XbaI T'CTAG_A
Cuts at: 1497 1497
Size: 4466
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1961 1961
Size: 4466
XmaI C'CCGG_G
Cuts at: 220 1486 220
Size: 1266 3200
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI Bpu10I BsiWI
BsmI BsmBI BspEI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SanDI SfiI SgrAI SrfI SspI
StuI SwaI XhoI XmnI ZraI