Restriction map for
pF1KB4823
(Circular) (Six-base) MAPSORT of: pF1KB4823.seq Check: 3515 from: 1 to: 5336
pF1KB4823 5336 bp
With 182 enzymes: SgfI *
January 16, 2014 17:30 ..
AccI GT'mk_AC
Cuts at: 2374 2374
Size: 5336
Acc65I G'GTAC_C
Cuts at: 2352 2352
Size: 5336
AclI AA'CG_TT
Cuts at: 5176 5176
Size: 5336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 690 1036 1435 2004 3161 3593 4340 4608
Size: 362 346 399 569 1157 432 747 268
Cuts at: 4608 328
Size: 1056
Fragments arranged by size:
1157 1056 747 569 432 399 362 346
268
AflII C'TTAA_G
Cuts at: 1578 1578
Size: 5336
AflIII A'CryG_T
Cuts at: 179 2117 2195 2688 3792 4769 179
Size: 1938 78 493 1104 977 746
Fragments arranged by size:
1938 1104 977 746 493 78
AgeI A'CCGG_T
Cuts at: 3724 3724
Size: 5336
AhdI GACnn_n'nnGTC
Cuts at: 180 180
Size: 5336
AleI CACnn'nnGTG
Cuts at: 151 151
Size: 5336
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1174 1174
Size: 5336
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 907 939 907
Size: 32 5304
AlwNI CAG_nnn'CTG
Cuts at: 2641 4208 2641
Size: 1567 3769
ApaI G_GGCC'C
Cuts at: 335 416 335
Size: 81 5255
ApaLI G'TGCA_C
Cuts at: 418 2143 4106 418
Size: 1725 1963 1648
Fragments arranged by size:
1963 1725 1648
ApoI r'AATT_y
Cuts at: 2340 4689 4945 2340
Size: 2349 256 2731
Fragments arranged by size:
2731 2349 256
AseI AT'TA_AT
Cuts at: 20 1241 20
Size: 1221 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
AvaI C'yCGr_G
Cuts at: 798 936 965 1854 2356 798
Size: 138 29 889 502 3778
Fragments arranged by size:
3778 889 502 138 29
BamHI G'GATC_C
Cuts at: 1816 2361 1816
Size: 545 4791
BanI G'GyrC_C
Cuts at: 744 2352 2766 3014 3049 744
Size: 1608 414 248 35 3031
Fragments arranged by size:
3031 1608 414 248 35
BanII G_rGCy'C
Cuts at: 223 335 416 475 688 1577 1844 2350 3380
Size: 112 81 59 213 889 267 506 1030
Cuts at: 3380 223
Size: 2179
Fragments arranged by size:
2179 1030 889 506 267 213 112 81
59
BbeI G_GCGC'C
Cuts at: 2770 3018 2770
Size: 248 5088
BbsI GAAGACnn'nnnn_
Cuts at: 1042 1042
Size: 5336
BbvCI CC'TCA_GC
Cuts at: 724 724
Size: 5336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1686 1720 1686
Size: 34 5302
BciVI GTATCCnnnnn_n'
Cuts at: 3234 3995 5336 3234
Size: 761 1341 3234
Fragments arranged by size:
3234 1341 761
BclI T'GATC_A
Cuts at: 1365 2856 1365
Size: 1491 3845
BglI GCCn_nnn'nGGC
Cuts at: 556 565 2242 556
Size: 9 1677 3650
Fragments arranged by size:
3650 1677 9
BglII A'GATC_T
Cuts at: 2851 4888 2851
Size: 2037 3299
BlpI GC'TnA_GC
Cuts at: 1825 2448 1825
Size: 623 4713
Bme1580I G_kGCm'C
Cuts at: 335 416 422 2147 2961 3054 4110 335
Size: 81 6 1725 814 93 1056 1561
Fragments arranged by size:
1725 1561 1056 814 93 81 6
BmrI ACTGGGnnnn_n'
Cuts at: 419 1034 2084 2548 2629 2964 5108 419
Size: 615 1050 464 81 335 2144 647
Fragments arranged by size:
2144 1050 647 615 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3703 3703
Size: 5336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 1187 1547 292
Size: 895 360 4081
Fragments arranged by size:
4081 895 360
Bpu10I CC'TnA_GC
Cuts at: 724 724
Size: 5336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 832 1360 2512 3391 3883 4181 543
Size: 289 528 1152 879 492 298 1698
Fragments arranged by size:
1698 1152 879 528 492 298 289
BsaI GGTCTCn'nnnn_
Cuts at: 1511 5040 1511
Size: 3529 1807
BsaAI yAC'GTr
Cuts at: 681 2689 681
Size: 2008 3328
BsaBI GATnn'nnATC
Cuts at: 6 2850 4887 6
Size: 2844 2037 455
Fragments arranged by size:
2844 2037 455
BsaHI Gr'CG_yC
Cuts at: 1018 2767 3015 3717 5213 1018
Size: 1749 248 702 1496 1141
Fragments arranged by size:
1749 1496 1141 702 248
BsaWI w'CCGG_w
Cuts at: 2525 3046 3724 3998 4145 4556 2525
Size: 521 678 274 147 411 3305
Fragments arranged by size:
3305 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 670 700 847 877 1117 1120 1147 1150 670
Size: 30 147 30 240 3 27 3 4856
Fragments arranged by size:
4856 240 147 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 758 1125 289
Size: 469 367 4500
Fragments arranged by size:
4500 469 367
BseYI C'CCAG_C
Cuts at: 761 1612 2088 2277 2761 3519 4096 4592 761
Size: 851 476 189 484 758 577 496 1505
Fragments arranged by size:
1505 851 758 577 496 484 476 189
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1552 1552
Size: 5336
BsiEI CG_ry'CG
Cuts at: 104 2878 2924 4132 104
Size: 2774 46 1208 1308
Fragments arranged by size:
2774 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 422 2147 2350 3128 3318 4110 422
Size: 1725 203 778 190 792 1648
Fragments arranged by size:
1725 1648 792 778 203 190
Bsp1286I G_dGCh'C
Cuts at: 223 335 416 422 475 688 1577 1844 2147
Size: 112 81 6 53 213 889 267 303
Cuts at: 2147 2350 2961 3054 3128 3318 3380 4110 223
Size: 203 611 93 74 190 62 730 1449
Fragments arranged by size:
1449 889 730 611 303 267 213 203
190 112 93 81 74 62 53 6
BspEI T'CCGG_A
Cuts at: 4556 4556
Size: 5336
BspHI T'CATG_A
Cuts at: 1175 4512 1175
Size: 3337 1999
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1059 1943 2386 2600 2902 3283 1059
Size: 884 443 214 302 381 3112
Fragments arranged by size:
3112 884 443 381 302 214
BsrBI CCG'CTC
Cuts at: 50 872 1755 3628 3682 5166 5331 50
Size: 822 883 1873 54 1484 165 55
Fragments arranged by size:
1873 1484 883 822 165 55 54
BsrDI GCAATG_nn'
Cuts at: 291 1989 3248 291
Size: 1698 1259 2379
Fragments arranged by size:
2379 1698 1259
BsrFI r'CCGG_y
Cuts at: 3334 3515 3724 3334
Size: 181 209 4946
Fragments arranged by size:
4946 209 181
BsrGI T'GTAC_A
Cuts at: 1439 1439
Size: 5336
BssSI C'ACGA_G
Cuts at: 3607 3965 3607
Size: 358 4978
BstAPI GCAn_nnn'nTGC
Cuts at: 2713 2713
Size: 5336
BstBI TT'CG_AA
Cuts at: 3697 3697
Size: 5336
BstEII G'GTnAC_C
Cuts at: 1114 1114
Size: 5336
BstXI CCAn_nnnn'nTGG
Cuts at: 1612 1612
Size: 5336
BstYI r'GATC_y
Cuts at: 1 1309 1375 1816 1916 2361 2828 2851 3186
Size: 1308 66 441 100 445 467 23 335
Cuts at: 3186 3432 4444 4530 4542 4888 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1308 1012 467 449 445 441 346 335
246 100 86 66 23 12
BtgI C'CryG_G
Cuts at: 107 569 1881 107
Size: 462 1312 3562
Fragments arranged by size:
3562 1312 462
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3286 3467 3630 3286
Size: 181 163 4992
Fragments arranged by size:
4992 181 163
BtsI GCAGTG_nn'
Cuts at: 1540 1552 1782 2579 1540
Size: 12 230 797 4297
Fragments arranged by size:
4297 797 230 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 222 257 222
Size: 35 5301
DraI TTT'AAA
Cuts at: 2335 2335
Size: 5336
DrdI GACnn_nn'nnGTC
Cuts at: 3042 3900 3042
Size: 858 4478
EaeI y'GGCC_r
Cuts at: 288 456 566 986 2921 3095 3486 3513 4858
Size: 168 110 420 1935 174 391 27 1345
Cuts at: 4858 288
Size: 766
Fragments arranged by size:
1935 1345 766 420 391 174 168 110
27
EagI C'GGCC_G
Cuts at: 2921 2921
Size: 5336
EarI CTCTTCn'nnn_
Cuts at: 640 3359 3569 640
Size: 2719 210 2407
Fragments arranged by size:
2719 2407 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 679 3544 3854 4000 5146 679
Size: 2865 310 146 1146 869
Fragments arranged by size:
2865 1146 869 310 146
EcoICRI GAG'CTC
Cuts at: 2348 2348
Size: 5336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 328 690 1036 1187 1435 1547 2004 3161
Size: 36 362 346 151 248 112 457 1157
Cuts at: 3161 3593 4340 4608 292
Size: 432 747 268 1020
Fragments arranged by size:
1157 1020 747 457 432 362 346 268
248 151 112 36
EcoNI CCTnn'n_nnAGG
Cuts at: 274 274
Size: 5336
EcoO109I rG'GnC_Cy
Cuts at: 331 578 720 1932 2010 2282 2475 331
Size: 247 142 1212 78 272 193 3192
Fragments arranged by size:
3192 1212 272 247 193 142 78
EcoRI G'AATT_C
Cuts at: 2340 2340
Size: 5336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 873 1604 3355 873
Size: 731 1751 2854
Fragments arranged by size:
2854 1751 731
FspI TGC'GCA
Cuts at: 974 3117 974
Size: 2143 3193
HaeII r_GCGC'y
Cuts at: 2770 3018 4040 5019 2770
Size: 248 1022 979 3087
Fragments arranged by size:
3087 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 589 621 847 879 1008 1040 1072
Size: 32 391 32 226 32 129 32 32
Cuts at: 1072 1104 1117 1149 1301 1333 1808 1840 2302
Size: 32 13 32 152 32 475 32 462
Cuts at: 2302 2334 3178 3208 3210 3240 166
Size: 32 844 30 2 30 2262
Fragments arranged by size:
2262 844 475 462 391 226 152 129
32 32 32 32 32 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 591 2028 2375 591
Size: 1437 347 3552
Fragments arranged by size:
3552 1437 347
HindIII A'AGCT_T
Cuts at: 952 952
Size: 5336
KasI G'GCGC_C
Cuts at: 2766 3014 2766
Size: 248 5088
KpnI G_GTAC'C
Cuts at: 2356 2356
Size: 5336
MluI A'CGCG_T
Cuts at: 2117 4769 2117
Size: 2652 2684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1946 4007 4191 1946
Size: 2061 184 3091
Fragments arranged by size:
3091 2061 184
MscI TGG'CCA
Cuts at: 290 458 568 3097 290
Size: 168 110 2529 2529
Fragments arranged by size:
2529 2529 168 110
MslI CAynn'nnrTG
Cuts at: 151 442 904 1834 3325 3452 151
Size: 291 462 930 1491 127 2035
Fragments arranged by size:
2035 1491 930 462 291 127
MspA1I CmG'CkG
Cuts at: 363 716 1056 1914 2447 2761 3121 4134 4379
Size: 353 340 858 533 314 360 1013 245
Cuts at: 4379 363
Size: 1320
Fragments arranged by size:
1320 1013 858 533 360 353 340 314
245
NaeI GCC'GGC
Cuts at: 3517 3517
Size: 5336
NarI GG'CG_CC
Cuts at: 2767 3015 2767
Size: 248 5088
NcoI C'CATG_G
Cuts at: 107 569 107
Size: 462 4874
NgoMIV G'CCGG_C
Cuts at: 3515 3515
Size: 5336
NspI r_CATG'y
Cuts at: 2389 2889 3796 2389
Size: 500 907 3929
Fragments arranged by size:
3929 907 500
PciI A'CATG_T
Cuts at: 3792 3792
Size: 5336
PfoI T'CCnGG_A
Cuts at: 1496 1496
Size: 5336
PmeI GTTT'AAAC
Cuts at: 2335 2335
Size: 5336
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 4475
Size: 32 5304
PpuMI rG'GwC_Cy
Cuts at: 578 720 1932 578
Size: 142 1212 3982
Fragments arranged by size:
3982 1212 142
PspOMI G'GGCC_C
Cuts at: 331 412 331
Size: 81 5255
PstI C_TGCA'G
Cuts at: 1559 1777 2383 3068 1559
Size: 218 606 685 3827
Fragments arranged by size:
3827 685 606 218
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5336
PvuII CAG'CTG
Cuts at: 363 1056 2761 3121 363
Size: 693 1705 360 2578
Fragments arranged by size:
2578 1705 693 360
RsrII CG'GwC_CG
Cuts at: 3531 3531
Size: 5336
SacI G_AGCT'C
Cuts at: 2350 2350
Size: 5336
SalI G'TCGA_C
Cuts at: 2373 2373
Size: 5336
SapI GCTCTTCn'nnn_
Cuts at: 640 3359 3569 640
Size: 2719 210 2407
Fragments arranged by size:
2719 2407 210
SbfI CC_TGCA'GG
Cuts at: 2383 2383
Size: 5336
ScaI AGT'ACT
Cuts at: 4853 4853
Size: 5336
SfcI C'TryA_G
Cuts at: 33 1223 1555 1619 1773 2379 3064 4057 4248
Size: 1190 332 64 154 606 685 993 191
Cuts at: 4248 33
Size: 1121
Fragments arranged by size:
1190 1121 993 685 606 332 191 154
64
SfiI GGCCn_nnn'nGGCC
Cuts at: 565 565
Size: 5336
SfoI GGC'GCC
Cuts at: 2768 3016 2768
Size: 248 5088
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
SmaI CCC'GGG
Cuts at: 967 2358 967
Size: 1391 3945
SmlI C'TyrA_G
Cuts at: 522 847 1339 1578 1854 2491 3406 3898 4160
Size: 325 492 239 276 637 915 492 262
Cuts at: 4160 522
Size: 1698
Fragments arranged by size:
1698 915 637 492 492 325 276 262
239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5336
SphI G_CATG'C
Cuts at: 2389 2889 2389
Size: 500 4836
SrfI GCCC'GGGC
Cuts at: 967 967
Size: 5336
StuI AGG'CCT
Cuts at: 520 520
Size: 5336
StyI C'CwwG_G
Cuts at: 107 188 569 959 1205 1605 2240 2470 2824
Size: 81 381 390 246 400 635 230 354
Cuts at: 2824 107
Size: 2619
Fragments arranged by size:
2619 635 400 390 381 354 246 230
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1138 1534 1655 2277 2919 3052 3511 3720 1138
Size: 396 121 622 642 133 459 209 2754
Fragments arranged by size:
2754 642 622 459 396 209 133 121
TatI w'GTAC_w
Cuts at: 389 1439 2123 4851 389
Size: 1050 684 2728 874
Fragments arranged by size:
2728 1050 874 684
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1082 1114 4853 4885 1082
Size: 32 3739 32 1533
Fragments arranged by size:
3739 1533 32 32
Tth111I GACn'n_nGTC
Cuts at: 582 1084 3133 582
Size: 502 2049 2785
Fragments arranged by size:
2785 2049 502
XbaI T'CTAG_A
Cuts at: 2367 2367
Size: 5336
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2831 2831
Size: 5336
XhoI C'TCGA_G
Cuts at: 1854 1854
Size: 5336
XmaI C'CCGG_G
Cuts at: 965 2356 965
Size: 1391 3945
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SspI SwaI XmnI ZraI