Restriction map for
pF1KB4835
(Circular) (Six-base) MAPSORT of: pF1KB4835.seq Check: 9125 from: 1 to: 4547
pF1KB4835 4547 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 791 852 1247 791
Size: 61 395 4091
Fragments arranged by size:
4091 395 61
AccI GT'mk_AC
Cuts at: 1585 1585
Size: 4547
Acc65I G'GTAC_C
Cuts at: 634 1563 634
Size: 929 3618
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 959 1447 2372 2804 3551 3819 634
Size: 325 488 925 432 747 268 1362
Fragments arranged by size:
1362 925 747 488 432 325 268
AflIII A'CryG_T
Cuts at: 833 1899 3003 3980 833
Size: 1066 1104 977 1400
Fragments arranged by size:
1400 1104 1066 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AleI CACnn'nnGTG
Cuts at: 154 154
Size: 4547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1324 1356 1324
Size: 32 4515
AlwNI CAG_nnn'CTG
Cuts at: 1335 1852 3419 1335
Size: 517 1567 2463
Fragments arranged by size:
2463 1567 517
ApaI G_GGCC'C
Cuts at: 272 272
Size: 4547
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 511 1551 3900 4156 511
Size: 1040 2349 256 902
Fragments arranged by size:
2349 1040 902 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 461 765 1304 1567 461
Size: 304 539 263 3441
Fragments arranged by size:
3441 539 304 263
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 145 634 1235 1563 1977 2225 2260 145
Size: 489 601 328 414 248 35 2432
Fragments arranged by size:
2432 601 489 414 328 248 35
BanII G_rGCy'C
Cuts at: 272 461 766 1311 1561 2591 272
Size: 189 305 545 250 1030 2228
Fragments arranged by size:
2228 1030 545 305 250 189
BbeI G_GCGC'C
Cuts at: 1981 2229 1981
Size: 248 4299
BbvCI CC'TCA_GC
Cuts at: 172 172
Size: 4547
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 307 341 682 716 307
Size: 34 341 34 4138
Fragments arranged by size:
4138 341 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BfrBI ATG'CAT
Cuts at: 387 387
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 276 276
Size: 4547
BglII A'GATC_T
Cuts at: 1125 2062 4099 1125
Size: 937 2037 1573
Fragments arranged by size:
2037 1573 937
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 148 225 272 1298 2172 2265 3321 148
Size: 77 47 1026 874 93 1056 1374
Fragments arranged by size:
1374 1056 1026 874 93 77 47
BmgBI CAC'GTC
Cuts at: 1422 1422
Size: 4547
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1759 1840 2175 4319 1009
Size: 750 81 335 2144 1237
Fragments arranged by size:
2144 1237 750 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 1156 1348 983
Size: 173 192 4182
Fragments arranged by size:
4182 192 173
Bpu10I CC'TnA_GC
Cuts at: 172 172
Size: 4547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1723 2602 3094 3392 1723
Size: 879 492 298 2878
Fragments arranged by size:
2878 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4251 4251
Size: 4547
BsaAI yAC'GTr
Cuts at: 485 1299 1900 485
Size: 814 601 3132
Fragments arranged by size:
3132 814 601
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 1978 2226 2928 4424 1978
Size: 248 702 1496 2101
Fragments arranged by size:
2101 1496 702 248
BsaWI w'CCGG_w
Cuts at: 350 1514 1736 2257 2935 3209 3356 3767 350
Size: 1164 222 521 678 274 147 411 1130
Fragments arranged by size:
1164 1130 678 521 411 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 332 362 753 783 1324 1354 332
Size: 30 391 30 541 30 3525
Fragments arranged by size:
3525 541 391 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 774 1263 1266 774
Size: 489 3 4055
Fragments arranged by size:
4055 489 3
BseYI C'CCAG_C
Cuts at: 344 530 710 1224 1972 2730 3307 3803 344
Size: 186 180 514 748 758 577 496 1088
Fragments arranged by size:
1088 758 748 577 514 496 186 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 974 1204 1321 1459 974
Size: 230 117 138 4062
Fragments arranged by size:
4062 230 138 117
BsiEI CG_ry'CG
Cuts at: 104 121 251 2089 2135 3343 104
Size: 17 130 1838 46 1208 1308
Fragments arranged by size:
1838 1308 1208 130 46 17
BsiHKAI G_wGCw'C
Cuts at: 766 1561 2339 2529 3321 766
Size: 795 778 190 792 1992
Fragments arranged by size:
1992 795 792 778 190
BsmI GAATG_Cn'
Cuts at: 705 705
Size: 4547
Bsp1286I G_dGCh'C
Cuts at: 148 225 272 461 766 1298 1311 1561 2172
Size: 77 47 189 305 532 13 250 611
Cuts at: 2172 2265 2339 2529 2591 3321 148
Size: 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 532 305 250 190 189
93 77 74 62 47 13
BspEI T'CCGG_A
Cuts at: 350 1514 3767 350
Size: 1164 2253 1130
Fragments arranged by size:
2253 1164 1130
BspHI T'CATG_A
Cuts at: 3723 3723
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 852 1247 1343 1597 1811 2113 2494 791
Size: 61 395 96 254 214 302 381 2844
Fragments arranged by size:
2844 395 381 302 254 214 96 61
BsrBI CCG'CTC
Cuts at: 50 2839 2893 4377 4542 50
Size: 2789 54 1484 165 55
Fragments arranged by size:
2789 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1086 2459 1086
Size: 1373 3174
BsrFI r'CCGG_y
Cuts at: 631 2545 2726 2935 631
Size: 1914 181 209 2243
Fragments arranged by size:
2243 1914 209 181
BssSI C'ACGA_G
Cuts at: 316 2818 3176 316
Size: 2502 358 1687
Fragments arranged by size:
2502 1687 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1344 1437 1924 1344
Size: 93 487 3967
Fragments arranged by size:
3967 487 93
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 388 388
Size: 4547
BstYI r'GATC_y
Cuts at: 1 1125 1572 2039 2062 2397 2643 3655 3741
Size: 1124 447 467 23 335 246 1012 86
Cuts at: 3741 3753 4099 1
Size: 12 346 449
Fragments arranged by size:
1124 1012 467 449 447 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 557 107
Size: 450 4097
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 780 2497 2678 2841 780
Size: 1717 181 163 2486
Fragments arranged by size:
2486 1717 181 163
BtsI GCAGTG_nn'
Cuts at: 212 816 1401 1790 212
Size: 604 585 389 2969
Fragments arranged by size:
2969 604 585 389
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 118 263 495 554 1058 1088 2132 2306 2697
Size: 145 232 59 504 30 1044 174 391
Cuts at: 2697 2724 4069 118
Size: 27 1345 596
Fragments arranged by size:
1345 1044 596 504 391 232 174 145
59 30 27
EagI C'GGCC_G
Cuts at: 118 2132 118
Size: 2014 2533
EarI CTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2755 3065 3211 4357 2755
Size: 310 146 1146 2945
Fragments arranged by size:
2945 1146 310 146
EcoICRI GAG'CTC
Cuts at: 764 1559 764
Size: 795 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 959 983 1156 1348 1447 2372 2804 3551
Size: 325 24 173 192 99 925 432 747
Cuts at: 3551 3819 634
Size: 268 1362
Fragments arranged by size:
1362 925 747 432 325 268 192 173
99 24
EcoO109I rG'GnC_Cy
Cuts at: 269 1043 1686 269
Size: 774 643 3130
Fragments arranged by size:
3130 774 643
EcoRI G'AATT_C
Cuts at: 511 1551 511
Size: 1040 3507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 1981 2229 3251 4230 1981
Size: 248 1022 979 2298
Fragments arranged by size:
2298 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 332 364 1110 1142 1293 1325 2389
Size: 32 122 32 746 32 151 32 1064
Cuts at: 2389 2419 2421 2451 178
Size: 30 2 30 2274
Fragments arranged by size:
2274 1064 746 151 122 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
KasI G'GCGC_C
Cuts at: 1977 2225 1977
Size: 248 4299
KpnI G_GTAC'C
Cuts at: 638 1567 638
Size: 929 3618
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 716 1304 3218 3402 716
Size: 588 1914 184 1861
Fragments arranged by size:
1914 1861 588 184
MscI TGG'CCA
Cuts at: 556 1090 2308 556
Size: 534 1218 2795
Fragments arranged by size:
2795 1218 534
MslI CAynn'nnrTG
Cuts at: 154 370 548 647 2536 2663 154
Size: 216 178 99 1889 127 2038
Fragments arranged by size:
2038 1889 216 178 127 99
MspA1I CmG'CkG
Cuts at: 710 957 1180 1251 1489 1658 1972 2332 3345
Size: 247 223 71 238 169 314 360 1013
Cuts at: 3345 3590 710
Size: 245 1667
Fragments arranged by size:
1667 1013 360 314 247 245 238 223
169 71
NaeI GCC'GGC
Cuts at: 2728 2728
Size: 4547
NarI GG'CG_CC
Cuts at: 1978 2226 1978
Size: 248 4299
NcoI C'CATG_G
Cuts at: 107 107
Size: 4547
NgoMIV G'CCGG_C
Cuts at: 2726 2726
Size: 4547
NsiI A_TGCA'T
Cuts at: 389 389
Size: 4547
NspI r_CATG'y
Cuts at: 837 1107 1600 2100 3007 837
Size: 270 493 500 907 2377
Fragments arranged by size:
2377 907 500 493 270
PasI CC'CwG_GG
Cuts at: 273 1290 273
Size: 1017 3530
PciI A'CATG_T
Cuts at: 833 3003 833
Size: 2170 2377
PflMI CCAn_nnn'nTGG
Cuts at: 584 1107 584
Size: 523 4024
PfoI T'CCnGG_A
Cuts at: 1364 1411 1364
Size: 47 4500
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PmlI CAC'GTG
Cuts at: 485 1299 485
Size: 814 3733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1310 1324 1342 1356 3686 3718 1310
Size: 14 18 14 2330 32 2139
Fragments arranged by size:
2330 2139 32 18 14 14
PpuMI rG'GwC_Cy
Cuts at: 1043 1043
Size: 4547
PspOMI G'GGCC_C
Cuts at: 268 268
Size: 4547
PstI C_TGCA'G
Cuts at: 1594 2279 1594
Size: 685 3862
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 710 957 1180 1251 1489 1972 2332 710
Size: 247 223 71 238 483 360 2925
Fragments arranged by size:
2925 483 360 247 238 223 71
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 766 1561 766
Size: 795 3752
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 4064 4064
Size: 4547
SexAI A'CCwGG_T
Cuts at: 476 476
Size: 4547
SfcI C'TryA_G
Cuts at: 33 1590 2275 3268 3459 33
Size: 1557 685 993 191 1121
Fragments arranged by size:
1557 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1979 2227 1979
Size: 248 4299
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 1306 1569 1306
Size: 263 4284
SmlI C'TyrA_G
Cuts at: 1702 2617 3109 3371 1702
Size: 915 492 262 2878
Fragments arranged by size:
2878 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 892 1133 892
Size: 241 4306
StyI C'CwwG_G
Cuts at: 107 414 875 1681 2035 107
Size: 307 461 806 354 2619
Fragments arranged by size:
2619 806 461 354 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 868 2130 2263 2722 2931 868
Size: 1262 133 459 209 2484
Fragments arranged by size:
2484 1262 459 209 133
TatI w'GTAC_w
Cuts at: 4062 4062
Size: 4547
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4515
Tth111I GACn'n_nGTC
Cuts at: 2344 2344
Size: 4547
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2042 2042
Size: 4547
XmaI C'CCGG_G
Cuts at: 1304 1567 1304
Size: 263 4284
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbsI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI