Restriction map for
pF1KB4848
(Circular) (Six-base) MAPSORT of: pF1KB4848.seq Check: 2306 from: 1 to: 4370
pF1KB4848 4370 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 323 1386 323
Size: 1063 3307
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2627 2980 3642 2195
Size: 432 353 662 2923
Fragments arranged by size:
2923 662 432 353
AfeI AGC'GCT
Cuts at: 262 262
Size: 4370
AflIII A'CryG_T
Cuts at: 1722 3522 3803 1722
Size: 1800 281 2289
Fragments arranged by size:
2289 1800 281
AgeI A'CCGG_T
Cuts at: 320 320
Size: 4370
AleI CACnn'nnGTG
Cuts at: 349 349
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 1193 1300 1675 3113 1193
Size: 107 375 1438 2450
Fragments arranged by size:
2450 1438 375 107
ApaLI G'TGCA_C
Cuts at: 292 3208 292
Size: 2916 1454
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 1390 1390
Size: 4370
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 315 348 1070 1103 315
Size: 33 722 33 3582
Fragments arranged by size:
3582 722 33 33
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 323 1386 1800 2048 2083 323
Size: 1063 414 248 35 2610
Fragments arranged by size:
2610 1063 414 248 35
BanII G_rGCy'C
Cuts at: 634 961 1069 1325 1360 1384 2414 634
Size: 327 108 256 35 24 1030 2590
Fragments arranged by size:
2590 1030 327 256 108 35 24
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 375 375
Size: 4370
BbvCI CC'TCA_GC
Cuts at: 1277 1277
Size: 4370
BciVI GTATCCnnnnn_n'
Cuts at: 1071 2268 3324 4370 1071
Size: 1197 1056 1046 1071
Fragments arranged by size:
1197 1071 1056 1046
BclI T'GATC_A
Cuts at: 929 1890 929
Size: 961 3409
BglI GCCn_nnn'nGGC
Cuts at: 1329 1329
Size: 4370
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 983 1301 1482 983
Size: 318 181 3871
Fragments arranged by size:
3871 318 181
Bme1580I G_kGCm'C
Cuts at: 296 1995 2088 3212 296
Size: 1699 93 1124 1454
Fragments arranged by size:
1699 1454 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 1222
Size: 4370
Bpu10I CC'TnA_GC
Cuts at: 1277 1277
Size: 4370
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1546 2425 3139 3437 343
Size: 1203 879 714 298 1276
Fragments arranged by size:
1276 1203 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 307 535 694 1168 4074 307
Size: 228 159 474 2906 603
Fragments arranged by size:
2906 603 474 228 159
BsaAI yAC'GTr
Cuts at: 1723 1723
Size: 4370
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 1801 2049 2751 4247 1801
Size: 248 702 1496 1924
Fragments arranged by size:
1924 1496 702 248
BsaWI w'CCGG_w
Cuts at: 320 1559 2080 2758 3169 3316 3590 320
Size: 1239 521 678 411 147 274 1100
Fragments arranged by size:
1239 1100 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 598 914 1225 598
Size: 316 311 3743
Fragments arranged by size:
3743 316 311
BseYI C'CCAG_C
Cuts at: 1186 1249 1263 1332 1795 2553 3218 3626 1186
Size: 63 14 69 463 758 665 408 1930
Fragments arranged by size:
1930 758 665 463 408 69 63 14
BsiEI CG_ry'CG
Cuts at: 104 906 1912 1958 3188 104
Size: 802 1006 46 1230 1286
Fragments arranged by size:
1286 1230 1006 802 46
BsiHKAI G_wGCw'C
Cuts at: 296 1069 1091 1384 2162 2352 3212 296
Size: 773 22 293 778 190 860 1454
Fragments arranged by size:
1454 860 778 773 293 190 22
BsiWI C'GTAC_G
Cuts at: 223 223
Size: 4370
BsmI GAATG_Cn'
Cuts at: 132 132
Size: 4370
Bsp1286I G_dGCh'C
Cuts at: 296 634 961 1069 1091 1325 1360 1384 1995
Size: 338 327 108 22 234 35 24 611
Cuts at: 1995 2088 2162 2352 2414 3212 296
Size: 93 74 190 62 798 1454
Fragments arranged by size:
1454 798 611 338 327 234 190 108
93 74 62 35 24 22
BspHI T'CATG_A
Cuts at: 2802 2802
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1060 1420 1634 1936 2317 1060
Size: 360 214 302 381 3113
Fragments arranged by size:
3113 381 360 302 214
BsrBI CCG'CTC
Cuts at: 50 278 697 2662 2716 4200 4365 50
Size: 228 419 1965 54 1484 165 55
Fragments arranged by size:
1965 1484 419 228 165 55 54
BsrDI GCAATG_nn'
Cuts at: 209 437 2282 209
Size: 228 1845 2297
Fragments arranged by size:
2297 1845 228
BsrFI r'CCGG_y
Cuts at: 320 1229 2368 2549 320
Size: 909 1139 181 2141
Fragments arranged by size:
2141 1139 909 181
BssSI C'ACGA_G
Cuts at: 940 2641 3349 940
Size: 1701 708 1961
Fragments arranged by size:
1961 1701 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1052 1747 1052
Size: 695 3675
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstEII G'GTnAC_C
Cuts at: 454 454
Size: 4370
BstXI CCAn_nnnn'nTGG
Cuts at: 351 1206 1332 351
Size: 855 126 3389
Fragments arranged by size:
3389 855 126
BstYI r'GATC_y
Cuts at: 1 1395 1862 1885 2220 2466 2772 2784 2870
Size: 1394 467 23 335 246 306 12 86
Cuts at: 2870 3922 1
Size: 1052 449
Fragments arranged by size:
1394 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 297 389 107
Size: 190 92 4088
Fragments arranged by size:
4088 190 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 1258 1613 1258
Size: 355 4015
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DraIII CAC_nnn'GTG
Cuts at: 795 795
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 3420 2076
Size: 1344 3026
EaeI y'GGCC_r
Cuts at: 236 1955 2129 2520 2547 3892 236
Size: 1719 174 391 27 1345 714
Fragments arranged by size:
1719 1345 714 391 174 27
EagI C'GGCC_G
Cuts at: 1955 1955
Size: 4370
EarI CTCTTCn'nnn_
Cuts at: 268 343 685 916 949 992 1213 2393 2603
Size: 75 342 231 33 43 221 1180 210
Cuts at: 2603 268
Size: 2035
Fragments arranged by size:
2035 1180 342 231 221 210 75 43
33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 3320 3466 4180 2578
Size: 742 146 714 2768
Fragments arranged by size:
2768 742 714 146
EcoICRI GAG'CTC
Cuts at: 1067 1382 1067
Size: 315 4055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2195 2627 2980 3642 1222
Size: 973 432 353 662 1950
Fragments arranged by size:
1950 973 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 168 1509 168
Size: 1341 3029
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 384 2389 384
Size: 2005 2365
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 264 369 1804 2052 3282 4053 264
Size: 105 1435 248 1230 771 581
Fragments arranged by size:
1435 1230 771 581 248 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2212 2242 2244 2274 2212
Size: 30 2 30 4308
Fragments arranged by size:
4308 30 30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 327 1390 327
Size: 1063 3307
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 740 3129 3313 740
Size: 2389 184 1797
Fragments arranged by size:
2389 1797 184
MscI TGG'CCA
Cuts at: 2131 2131
Size: 4370
MslI CAynn'nnrTG
Cuts at: 349 733 880 1045 2359 2486 349
Size: 384 147 165 1314 127 2233
Fragments arranged by size:
2233 1314 384 165 147 127
MspA1I CmG'CkG
Cuts at: 987 1190 1481 1795 2155 2939 3184 987
Size: 203 291 314 360 784 245 2173
Fragments arranged by size:
2173 784 360 314 291 245 203
NaeI GCC'GGC
Cuts at: 1231 2551 1231
Size: 1320 3050
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NcoI C'CATG_G
Cuts at: 107 389 107
Size: 282 4088
NgoMIV G'CCGG_C
Cuts at: 1229 2549 1229
Size: 1320 3050
NspI r_CATG'y
Cuts at: 666 1423 1923 3526 666
Size: 757 500 1603 1510
Fragments arranged by size:
1603 1510 757 500
PasI CC'CwG_GG
Cuts at: 164 164
Size: 4370
PciI A'CATG_T
Cuts at: 3522 3522
Size: 4370
PflMI CCAn_nnn'nTGG
Cuts at: 144 795 144
Size: 651 3719
PfoI T'CCnGG_A
Cuts at: 308 308
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 835 867 2816 2848 835
Size: 32 1949 32 2357
Fragments arranged by size:
2357 1949 32 32
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 4370
PstI C_TGCA'G
Cuts at: 1417 2102 1417
Size: 685 3685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 987 1190 1795 2155 987
Size: 203 605 360 3202
Fragments arranged by size:
3202 605 360 203
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1069 1384 1069
Size: 315 4055
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SanDI GG'GwC_CC
Cuts at: 168 168
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 268 949 2393 2603 268
Size: 681 1444 210 2035
Fragments arranged by size:
2035 1444 681 210
SbfI CC_TGCA'GG
Cuts at: 1417 1417
Size: 4370
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 1413 2098 3066 3257 33
Size: 1380 685 968 191 1146
Fragments arranged by size:
1380 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 358 1525 2440 3154 3416 358
Size: 1167 915 714 262 1312
Fragments arranged by size:
1312 1167 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StuI AGG'CCT
Cuts at: 802 802
Size: 4370
StyI C'CwwG_G
Cuts at: 107 389 1121 1199 1325 1504 1858 107
Size: 282 732 78 126 179 354 2619
Fragments arranged by size:
2619 732 354 282 179 126 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 559 1200 1324 1953 2086 2545 2754 559
Size: 641 124 629 133 459 209 2175
Fragments arranged by size:
2175 641 629 459 209 133 124
TatI w'GTAC_w
Cuts at: 3885 3885
Size: 4370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 346 378 477 509 3887 3919 346
Size: 32 99 32 3378 32 797
Fragments arranged by size:
3378 797 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1020 1401 1020
Size: 381 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1359 1865 1359
Size: 506 3864
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BcgI BfrBI BmgBI BmtI BsaXI BsgI BsmBI
BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI