Restriction map for
pF1KB4862
(Circular) (Six-base) MAPSORT of: pF1KB4862.seq Check: 6410 from: 1 to: 4568
pF1KB4862 4568 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 1544 1544
Size: 4568
AccI GT'mk_AC
Cuts at: 518 1606 518
Size: 1088 3480
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2825 3178 3840 2393
Size: 432 353 662 3121
Fragments arranged by size:
3121 662 432 353
AflIII A'CryG_T
Cuts at: 944 1469 1920 3720 4001 944
Size: 525 451 1800 281 1511
Fragments arranged by size:
1800 1511 525 451 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 570 570
Size: 4568
AlwNI CAG_nnn'CTG
Cuts at: 1500 1873 3311 1500
Size: 373 1438 2757
Fragments arranged by size:
2757 1438 373
ApaLI G'TGCA_C
Cuts at: 3406 3406
Size: 4568
ApoI r'AATT_y
Cuts at: 499 1324 1572 3921 4177 499
Size: 825 248 2349 256 890
Fragments arranged by size:
2349 890 825 256 248
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 580 976 1588 580
Size: 396 612 3560
Fragments arranged by size:
3560 612 396
BamHI G'GATC_C
Cuts at: 431 1593 431
Size: 1162 3406
BanI G'GyrC_C
Cuts at: 1144 1584 1998 2246 2281 1144
Size: 440 414 248 35 3431
Fragments arranged by size:
3431 440 414 248 35
BanII G_rGCy'C
Cuts at: 773 827 1582 2612 773
Size: 54 755 1030 2729
Fragments arranged by size:
2729 1030 755 54
BbeI G_GCGC'C
Cuts at: 2002 2250 2002
Size: 248 4320
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3522 4568 2466
Size: 1056 1046 2466
Fragments arranged by size:
2466 1056 1046
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4568
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 1680 1680
Size: 4568
Bme1580I G_kGCm'C
Cuts at: 1147 2193 2286 3410 1147
Size: 1046 93 1124 2305
Fragments arranged by size:
2305 1124 1046 93
BmrI ACTGGGnnnn_n'
Cuts at: 436 486 735 1029 1780 1861 2196 4340 436
Size: 50 249 294 751 81 335 2144 664
Fragments arranged by size:
2144 751 664 335 294 249 81 50
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 568 846 895 2935 568
Size: 278 49 2040 2201
Fragments arranged by size:
2201 2040 278 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1062 865
Size: 197 4371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 820 1744 2623 3337 3635 362
Size: 458 924 879 714 298 1295
Fragments arranged by size:
1295 924 879 714 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 866 4272 866
Size: 3406 1162
BsaAI yAC'GTr
Cuts at: 1470 1921 1470
Size: 451 4117
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 1541 1999 2247 2949 4445 1541
Size: 458 248 702 1496 1664
Fragments arranged by size:
1664 1496 702 458 248
BsaWI w'CCGG_w
Cuts at: 1757 2278 2956 3367 3514 3788 1757
Size: 521 678 411 147 274 2537
Fragments arranged by size:
2537 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 948 978 948
Size: 30 4538
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 817 1534 817
Size: 717 3851
BseYI C'CCAG_C
Cuts at: 130 678 772 1319 1503 1993 2751 3416 3824
Size: 548 94 547 184 490 758 665 408
Cuts at: 3824 130
Size: 874
Fragments arranged by size:
874 758 665 548 547 490 408 184
94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 1510 353
Size: 1157 3411
BsiEI CG_ry'CG
Cuts at: 104 2110 2156 3386 104
Size: 2006 46 1230 1286
Fragments arranged by size:
2006 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 827 1582 2360 2550 3410 827
Size: 755 778 190 860 1985
Fragments arranged by size:
1985 860 778 755 190
Bsp1286I G_dGCh'C
Cuts at: 773 827 1147 1582 2193 2286 2360 2550 2612
Size: 54 320 435 611 93 74 190 62
Cuts at: 2612 3410 773
Size: 798 1931
Fragments arranged by size:
1931 798 611 435 320 190 93 74
62 54
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1499 1618 1832 2134 2515 1499
Size: 119 214 302 381 3552
Fragments arranged by size:
3552 381 302 214 119
BsrBI CCG'CTC
Cuts at: 50 2860 2914 4398 4563 50
Size: 2810 54 1484 165 55
Fragments arranged by size:
2810 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2480 2480
Size: 4568
BsrFI r'CCGG_y
Cuts at: 2566 2747 2566
Size: 181 4387
BssSI C'ACGA_G
Cuts at: 2839 3547 2839
Size: 708 3860
BstAPI GCAn_nnn'nTGC
Cuts at: 1500 1533 1945 1500
Size: 33 412 4123
Fragments arranged by size:
4123 412 33
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstYI r'GATC_y
Cuts at: 1 204 431 629 1593 2060 2083 2418 2664
Size: 203 227 198 964 467 23 335 246
Cuts at: 2664 2970 2982 3068 4120 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 964 467 449 335 306 246 227
203 198 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1517 1517
Size: 4568
BtgI C'CryG_G
Cuts at: 1410 1410
Size: 4568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 176 457 1239 1811 176
Size: 281 782 572 2933
Fragments arranged by size:
2933 782 572 281
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 1195 2274 3618 1195
Size: 1079 1344 2145
Fragments arranged by size:
2145 1344 1079
EaeI y'GGCC_r
Cuts at: 1352 2153 2327 2718 2745 4090 1352
Size: 801 174 391 27 1345 1830
Fragments arranged by size:
1830 1345 801 391 174 27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 815 2591 2801 815
Size: 1776 210 2582
Fragments arranged by size:
2582 1776 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2776 3518 3664 4378 2776
Size: 742 146 714 2966
Fragments arranged by size:
2966 742 714 146
EcoICRI GAG'CTC
Cuts at: 825 1580 825
Size: 755 3813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1062 2393 2825 3178 3840 865
Size: 197 1331 432 353 662 1593
Fragments arranged by size:
1593 1331 662 432 353 197
EcoNI CCTnn'n_nnAGG
Cuts at: 1183 1183
Size: 4568
EcoO109I rG'GnC_Cy
Cuts at: 184 1707 184
Size: 1523 3045
EcoRI G'AATT_C
Cuts at: 499 1324 1572 499
Size: 825 248 3495
Fragments arranged by size:
3495 825 248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1164 2587 1164
Size: 1423 3145
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4568
HaeII r_GCGC'y
Cuts at: 160 2002 2250 3480 4251 160
Size: 1842 248 1230 771 477
Fragments arranged by size:
1842 1230 771 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 416 448 789 821 841 873 948
Size: 32 117 32 341 32 20 32 75
Cuts at: 948 980 2410 2440 2442 2472 267
Size: 32 1430 30 2 30 2363
Fragments arranged by size:
2363 1430 341 117 75 32 32 32
32 32 30 30 20 2
HincII GTy'rAC
Cuts at: 675 1607 675
Size: 932 3636
KasI G'GCGC_C
Cuts at: 1998 2246 1998
Size: 248 4320
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3327 3511 3327
Size: 184 4384
MscI TGG'CCA
Cuts at: 1354 2329 1354
Size: 975 3593
MslI CAynn'nnrTG
Cuts at: 949 2557 2684 949
Size: 1608 127 2833
Fragments arranged by size:
2833 1608 127
MspA1I CmG'CkG
Cuts at: 1248 1304 1456 1679 1993 2353 3137 3382 1248
Size: 56 152 223 314 360 784 245 2434
Fragments arranged by size:
2434 784 360 314 245 223 152 56
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 4568
NarI GG'CG_CC
Cuts at: 1999 2247 1999
Size: 248 4320
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 4568
NspI r_CATG'y
Cuts at: 739 948 1621 2121 3724 739
Size: 209 673 500 1603 1583
Fragments arranged by size:
1603 1583 673 500 209
PasI CC'CwG_GG
Cuts at: 561 561
Size: 4568
PciI A'CATG_T
Cuts at: 944 3720 944
Size: 2776 1792
PflMI CCAn_nnn'nTGG
Cuts at: 1361 1361
Size: 4568
PfoI T'CCnGG_A
Cuts at: 1039 1039
Size: 4568
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 184 184
Size: 4568
PstI C_TGCA'G
Cuts at: 171 638 1073 1496 1615 2300 171
Size: 467 435 423 119 685 2439
Fragments arranged by size:
2439 685 467 435 423 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 1248 1304 1993 2353 1248
Size: 56 689 360 3463
Fragments arranged by size:
3463 689 360 56
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 827 1582 827
Size: 755 3813
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 815 2591 2801 815
Size: 1776 210 2582
Fragments arranged by size:
2582 1776 210
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 167 634 1069 1492 1611 2296 3264 3455
Size: 134 467 435 423 119 685 968 191
Cuts at: 3455 33
Size: 1146
Fragments arranged by size:
1146 968 685 467 435 423 191 134
119
SfoI GGC'GCC
Cuts at: 2000 2248 2000
Size: 248 4320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4568
SmlI C'TyrA_G
Cuts at: 377 580 835 1723 2638 3352 3614 377
Size: 203 255 888 915 714 262 1331
Fragments arranged by size:
1331 915 888 714 262 255 203
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 739 1621 2121 739
Size: 882 500 3186
Fragments arranged by size:
3186 882 500
StyI C'CwwG_G
Cuts at: 1139 1702 2056 1139
Size: 563 354 3651
Fragments arranged by size:
3651 563 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 468 849 1048 2151 2284 2743 2952 468
Size: 381 199 1103 133 459 209 2084
Fragments arranged by size:
2084 1103 459 381 209 199 133
TatI w'GTAC_w
Cuts at: 4083 4083
Size: 4568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 951 983 4085 4117 951
Size: 32 3102 32 1402
Fragments arranged by size:
3102 1402 32 32
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 624 1599 624
Size: 975 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1247 2063 1247
Size: 816 3752
XhoI C'TCGA_G
Cuts at: 580 580
Size: 4568
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4568
XmnI GAAnn'nnTTC
Cuts at: 499 499
Size: 4568
ZraI GAC'GTC
Cuts at: 1542 1542
Size: 4568
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI