Restriction map for
pF1KB4875
(Circular) (Six-base) MAPSORT of: pF1KB4875.seq Check: 1984 from: 1 to: 6239
pF1KB4875 6239 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 3277 3277
Size: 6239
Acc65I G'GTAC_C
Cuts at: 1056 3255 1056
Size: 2199 4040
AclI AA'CG_TT
Cuts at: 6079 6079
Size: 6239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 4064 4496 4849 5511 533
Size: 3531 432 353 662 1261
Fragments arranged by size:
3531 1261 662 432 353
AfeI AGC'GCT
Cuts at: 1640 2245 1640
Size: 605 5634
AflIII A'CryG_T
Cuts at: 1328 1475 3591 5391 5672 1328
Size: 147 2116 1800 281 1895
Fragments arranged by size:
2116 1895 1800 281 147
AleI CACnn'nnGTG
Cuts at: 1851 2236 1851
Size: 385 5854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1942 1942
Size: 6239
AlwNI CAG_nnn'CTG
Cuts at: 421 1713 2046 2313 2349 2476 2529 2569 3019
Size: 1292 333 267 36 127 53 40 450
Cuts at: 3019 3544 4982 421
Size: 525 1438 1678
Fragments arranged by size:
1678 1438 1292 525 450 333 267 127
53 40 36
ApaI G_GGCC'C
Cuts at: 253 826 965 1251 1819 2469 253
Size: 573 139 286 568 650 4023
Fragments arranged by size:
4023 650 573 568 286 139
ApaLI G'TGCA_C
Cuts at: 5077 5077
Size: 6239
ApoI r'AATT_y
Cuts at: 3243 5592 5848 3243
Size: 2349 256 3634
Fragments arranged by size:
3634 2349 256
AscI GG'CGCG_CC
Cuts at: 2128 2284 2128
Size: 156 6083
AseI AT'TA_AT
Cuts at: 20 20
Size: 6239
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6239
AvaI C'yCGr_G
Cuts at: 613 1811 1873 2278 2329 2948 3259 613
Size: 1198 62 405 51 619 311 3593
Fragments arranged by size:
3593 1198 619 405 311 62 51
BamHI G'GATC_C
Cuts at: 3264 3264
Size: 6239
BanI G'GyrC_C
Cuts at: 591 874 931 1005 1056 1796 1822 1869 1926
Size: 283 57 74 51 740 26 47 57
Cuts at: 1926 1971 2358 2790 3255 3669 3917 3952 591
Size: 45 387 432 465 414 248 35 2878
Fragments arranged by size:
2878 740 465 432 414 387 283 248
74 57 57 51 47 45 35 26
BanII G_rGCy'C
Cuts at: 139 253 495 614 826 965 1021 1042 1144
Size: 114 242 119 212 139 56 21 102
Cuts at: 1144 1251 1819 1846 1899 2469 3214 3253 4283
Size: 107 568 27 53 570 745 39 1030
Cuts at: 4283 139
Size: 2095
Fragments arranged by size:
2095 1030 745 570 568 242 212 139
119 114 107 102 56 53 39 27
21
BbeI G_GCGC'C
Cuts at: 1009 1800 1826 1873 1975 2362 2794 3673 3921
Size: 791 26 47 102 387 432 879 248
Cuts at: 3921 1009
Size: 3327
Fragments arranged by size:
3327 879 791 432 387 248 102 47
26
BbvCI CC'TCA_GC
Cuts at: 2800 2800
Size: 6239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2064 2098 2320 2354 90
Size: 34 1940 34 222 34 3975
Fragments arranged by size:
3975 1940 222 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4137 5193 6239 4137
Size: 1056 1046 4137
Fragments arranged by size:
4137 1056 1046
BclI T'GATC_A
Cuts at: 3759 3759
Size: 6239
BglI GCCn_nnn'nGGC
Cuts at: 204 960 1133 1391 1894 1916 1940 2201 2263
Size: 756 173 258 503 22 24 261 62
Cuts at: 2263 2457 3229 204
Size: 194 772 3214
Fragments arranged by size:
3214 772 756 503 261 258 194 173
62 24 22
BglII A'GATC_T
Cuts at: 3754 5791 3754
Size: 2037 4202
BlpI GC'TnA_GC
Cuts at: 1015 1041 1093 3008 3351 1015
Size: 26 52 1915 343 3903
Fragments arranged by size:
3903 1915 343 52 26
Bme1580I G_kGCm'C
Cuts at: 253 318 324 594 665 826 877 965 1251
Size: 65 6 270 71 161 51 88 286
Cuts at: 1251 1497 1819 1931 2469 3864 3957 5081 253
Size: 246 322 112 538 1395 93 1124 1411
Fragments arranged by size:
1411 1395 1124 538 322 286 270 246
161 112 93 88 71 65 51 6
BmgBI CAC'GTC
Cuts at: 468 2016 468
Size: 1548 4691
BmrI ACTGGGnnnn_n'
Cuts at: 2647 2708 2756 3451 3532 3867 6011 2647
Size: 61 48 695 81 335 2144 2875
Fragments arranged by size:
2875 2144 695 335 81 61 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 549 2931 3057 4606 549
Size: 2382 126 1549 2182
Fragments arranged by size:
2382 2182 1549 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 754 1015 2546 2902 2956 3133 718
Size: 36 261 1531 356 54 177 3824
Fragments arranged by size:
3824 1531 356 261 177 54 36
Bpu10I CC'TnA_GC
Cuts at: 1898 2800 3061 1898
Size: 902 261 5076
Fragments arranged by size:
5076 902 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 3415 4294 5008 5306 460
Size: 2955 879 714 298 1393
Fragments arranged by size:
2955 1393 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 506 2152 2972 3045 5943 506
Size: 1646 820 73 2898 802
Fragments arranged by size:
2898 1646 820 802 73
BsaAI yAC'GTr
Cuts at: 1002 1329 1476 2238 2821 3592 1002
Size: 327 147 762 583 771 3649
Fragments arranged by size:
3649 771 762 583 327 147
BsaBI GATnn'nnATC
Cuts at: 6 3753 5790 6
Size: 3747 2037 455
Fragments arranged by size:
3747 2037 455
BsaHI Gr'CG_yC
Cuts at: 1006 1797 1823 1870 1972 2216 2359 2791 3670
Size: 791 26 47 102 244 143 432 879
Cuts at: 3670 3918 4620 6116 1006
Size: 248 702 1496 1129
Fragments arranged by size:
1496 1129 879 791 702 432 248 244
143 102 47 26
BsaWI w'CCGG_w
Cuts at: 917 1376 3428 3949 4627 5038 5185 5459 917
Size: 459 2052 521 678 411 147 274 1697
Fragments arranged by size:
2052 1697 678 521 459 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1141 1171 2875 2905 2929 2959 3173 3203 1141
Size: 30 1704 30 24 30 214 30 4177
Fragments arranged by size:
4177 1704 214 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 715 865 1162 1165 1363 2599 2671 3130 3133
Size: 150 297 3 198 1236 72 459 3
Cuts at: 3133 3178 715
Size: 45 3776
Fragments arranged by size:
3776 1236 459 297 198 150 72 45
3 3
BseYI C'CCAG_C
Cuts at: 393 819 1021 1206 1250 1938 2317 2342 3207
Size: 426 202 185 44 688 379 25 865
Cuts at: 3207 3664 4422 5087 5495 393
Size: 457 758 665 408 1137
Fragments arranged by size:
1137 865 758 688 665 457 426 408
379 202 185 44 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 865 1491 2500 2515 2587 3194 653
Size: 212 626 1009 15 72 607 3698
Fragments arranged by size:
3698 1009 626 607 212 72 15
BsiEI CG_ry'CG
Cuts at: 104 686 1661 1952 1986 2064 2403 3781 3827
Size: 582 975 291 34 78 339 1378 46
Cuts at: 3827 5057 104
Size: 1230 1286
Fragments arranged by size:
1378 1286 1230 975 582 339 291 78
46 34
BsiHKAI G_wGCw'C
Cuts at: 614 740 1001 3253 4031 4221 5081 614
Size: 126 261 2252 778 190 860 1772
Fragments arranged by size:
2252 1772 860 778 261 190 126
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 2254
Size: 6239
Bsp1286I G_dGCh'C
Cuts at: 139 253 318 324 495 594 614 665 740
Size: 114 65 6 171 99 20 51 75
Cuts at: 740 826 877 965 1001 1021 1042 1144 1251
Size: 86 51 88 36 20 21 102 107
Cuts at: 1251 1497 1819 1846 1899 1931 2469 3214 3253
Size: 246 322 27 53 32 538 745 39
Cuts at: 3253 3864 3957 4031 4221 4283 5081 139
Size: 611 93 74 190 62 798 1297
Fragments arranged by size:
1297 798 745 611 538 322 246 190
171 114 107 102 99 93 88 86
75 74 65 62 53 51 51 39
36 32 27 21 20 20 6
BspEI T'CCGG_A
Cuts at: 917 1376 917
Size: 459 5780
BspHI T'CATG_A
Cuts at: 4671 4671
Size: 6239
BspMI ACCTGCnnnn'nnnn_
Cuts at: 357 587 1721 3089 3289 3503 3805 4186 357
Size: 230 1134 1368 200 214 302 381 2410
Fragments arranged by size:
2410 1368 1134 381 302 230 214 200
BsrBI CCG'CTC
Cuts at: 50 1460 1907 2399 4531 4585 6069 6234 50
Size: 1410 447 492 2132 54 1484 165 55
Fragments arranged by size:
2132 1484 1410 492 447 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4151 4151
Size: 6239
BsrFI r'CCGG_y
Cuts at: 121 1179 1660 2402 2456 3146 4237 4418 121
Size: 1058 481 742 54 690 1091 181 1942
Fragments arranged by size:
1942 1091 1058 742 690 481 181 54
BsrGI T'GTAC_A
Cuts at: 1157 2429 1157
Size: 1272 4967
BssHII G'CGCG_C
Cuts at: 1669 1863 2128 2284 1669
Size: 194 265 156 5624
Fragments arranged by size:
5624 265 194 156
BssSI C'ACGA_G
Cuts at: 1312 4510 5218 1312
Size: 3198 708 2333
Fragments arranged by size:
3198 2333 708
BstAPI GCAn_nnn'nTGC
Cuts at: 477 1713 2529 3616 477
Size: 1236 816 1087 3100
Fragments arranged by size:
3100 1236 1087 816
BstBI TT'CG_AA
Cuts at: 4600 4600
Size: 6239
BstEII G'GTnAC_C
Cuts at: 1737 2499 1737
Size: 762 5477
BstXI CCAn_nnnn'nTGG
Cuts at: 1426 3207 1426
Size: 1781 4458
BstYI r'GATC_y
Cuts at: 1 2472 2574 3264 3731 3754 4089 4335 4641
Size: 2471 102 690 467 23 335 246 306
Cuts at: 4641 4653 4739 5791 1
Size: 12 86 1052 449
Fragments arranged by size:
2471 1052 690 467 449 335 306 246
102 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2984 2984
Size: 6239
BtgI C'CryG_G
Cuts at: 927 967 1060 1685 2058 2064 2111 2148 927
Size: 40 93 625 373 6 47 37 5018
Fragments arranged by size:
5018 625 373 93 47 40 37 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 287 1226 4189 4370 4533 287
Size: 939 2963 181 163 1993
Fragments arranged by size:
2963 1993 939 181 163
BtsI GCAGTG_nn'
Cuts at: 388 670 839 2877 3482 388
Size: 282 169 2038 605 3145
Fragments arranged by size:
3145 2038 605 282 169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2807 2842 2807
Size: 35 6204
DraI TTT'AAA
Cuts at: 3238 3238
Size: 6239
DraIII CAC_nnn'GTG
Cuts at: 928 928
Size: 6239
DrdI GACnn_nn'nnGTC
Cuts at: 1746 3945 5289 1746
Size: 2199 1344 2696
Fragments arranged by size:
2696 2199 1344
EaeI y'GGCC_r
Cuts at: 683 1532 1631 1658 1662 1688 1724 1949 1983
Size: 849 99 27 4 26 36 225 34
Cuts at: 1983 2061 2103 2304 2400 2579 2687 3230 3824
Size: 78 42 201 96 179 108 543 594
Cuts at: 3824 3998 4389 4416 5761 683
Size: 174 391 27 1345 1161
Fragments arranged by size:
1345 1161 849 594 543 391 225 201
179 174 108 99 96 78 42 36
34 27 27 26 4
EagI C'GGCC_G
Cuts at: 683 1658 1949 1983 2061 2400 3824 683
Size: 975 291 34 78 339 1424 3098
Fragments arranged by size:
3098 1424 975 339 291 78 34
EarI CTCTTCn'nnn_
Cuts at: 329 376 4262 4472 329
Size: 47 3886 210 2096
Fragments arranged by size:
3886 2096 210 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1601 3112 3175 4447 5189 5335 6049 1601
Size: 1511 63 1272 742 146 714 1791
Fragments arranged by size:
1791 1511 1272 742 714 146 63
EcoICRI GAG'CTC
Cuts at: 612 3251 612
Size: 2639 3600
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 718 754 1015 2546 2902 2956 3133 4064
Size: 185 36 261 1531 356 54 177 931
Cuts at: 4064 4496 4849 5511 533
Size: 432 353 662 1261
Fragments arranged by size:
1531 1261 931 662 432 356 353 261
185 177 54 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2740 3085 3115 2740
Size: 345 30 5864
Fragments arranged by size:
5864 345 30
EcoO109I rG'GnC_Cy
Cuts at: 206 249 290 539 961 962 1591 1815 2096
Size: 43 41 249 422 1 629 224 281
Cuts at: 2096 2465 2466 2566 2649 2804 2876 3078 3378
Size: 369 1 100 83 155 72 202 300
Cuts at: 3378 206
Size: 3067
Fragments arranged by size:
3067 629 422 369 300 281 249 224
202 155 100 83 72 43 41 1
1
EcoRI G'AATT_C
Cuts at: 3243 3243
Size: 6239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4258 4258
Size: 6239
FseI GG_CCGG'CC
Cuts at: 1664 2406 1664
Size: 742 5497
FspI TGC'GCA
Cuts at: 262 4020 262
Size: 3758 2481
HaeII r_GCGC'y
Cuts at: 1009 1386 1642 1800 1826 1873 1975 2176 2247
Size: 377 256 158 26 47 102 201 71
Cuts at: 2247 2362 2794 3673 3921 5151 5922 1009
Size: 115 432 879 248 1230 771 1326
Fragments arranged by size:
1326 1230 879 771 432 377 256 248
201 158 115 102 71 47 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2929 2948 2961 2980 4081 4111 4113 4143 2929
Size: 19 13 19 1101 30 2 30 5025
Fragments arranged by size:
5025 1101 30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 3278 3278
Size: 6239
KasI G'GCGC_C
Cuts at: 1005 1796 1822 1869 1971 2358 2790 3669 3917
Size: 791 26 47 102 387 432 879 248
Cuts at: 3917 1005
Size: 3327
Fragments arranged by size:
3327 879 791 432 387 248 102 47
26
KpnI G_GTAC'C
Cuts at: 1060 3259 1060
Size: 2199 4040
MfeI C'AATT_G
Cuts at: 1998 1998
Size: 6239
MluI A'CGCG_T
Cuts at: 5672 5672
Size: 6239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1125 2621 2840 4998 5182 1125
Size: 1496 219 2158 184 2182
Fragments arranged by size:
2182 2158 1496 219 184
MscI TGG'CCA
Cuts at: 1633 1690 1726 2689 4000 1633
Size: 57 36 963 1311 3872
Fragments arranged by size:
3872 1311 963 57 36
MslI CAynn'nnrTG
Cuts at: 112 1851 1924 2236 4228 4355 112
Size: 1739 73 312 1992 127 1996
Fragments arranged by size:
1996 1992 1739 312 127 73
MspA1I CmG'CkG
Cuts at: 213 266 384 866 1014 1025 1840 2046 2066
Size: 53 118 482 148 11 815 206 20
Cuts at: 2066 2139 2338 2526 3207 3350 3664 4024 4808
Size: 73 199 188 681 143 314 360 784
Cuts at: 4808 5053 213
Size: 245 1399
Fragments arranged by size:
1399 815 784 681 482 360 314 245
206 199 188 148 143 118 73 53
20 11
NaeI GCC'GGC
Cuts at: 123 1662 2404 2458 4420 123
Size: 1539 742 54 1962 1942
Fragments arranged by size:
1962 1942 1539 742 54
NarI GG'CG_CC
Cuts at: 1006 1797 1823 1870 1972 2359 2791 3670 3918
Size: 791 26 47 102 387 432 879 248
Cuts at: 3918 1006
Size: 3327
Fragments arranged by size:
3327 879 791 432 387 248 102 47
26
NcoI C'CATG_G
Cuts at: 1685 1685
Size: 6239
NgoMIV G'CCGG_C
Cuts at: 121 1660 2402 2456 4418 121
Size: 1539 742 54 1962 1942
Fragments arranged by size:
1962 1942 1539 742 54
NruI TCG'CGA
Cuts at: 2012 2012
Size: 6239
NspI r_CATG'y
Cuts at: 3292 3792 5395 3292
Size: 500 1603 4136
Fragments arranged by size:
4136 1603 500
PasI CC'CwG_GG
Cuts at: 1785 1785
Size: 6239
PciI A'CATG_T
Cuts at: 5391 5391
Size: 6239
PflMI CCAn_nnn'nTGG
Cuts at: 1686 2112 2569 2974 3019 1686
Size: 426 457 405 45 4906
Fragments arranged by size:
4906 457 426 405 45
PfoI T'CCnGG_A
Cuts at: 695 1418 695
Size: 723 5516
PmeI GTTT'AAAC
Cuts at: 3238 3238
Size: 6239
PmlI CAC'GTG
Cuts at: 1002 1329 1476 2238 1002
Size: 327 147 762 5003
Fragments arranged by size:
5003 762 327 147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 703 735 1306 1338 4685 4717 703
Size: 32 571 32 3347 32 2225
Fragments arranged by size:
3347 2225 571 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 539 2096 2876 3078 539
Size: 1557 780 202 3700
Fragments arranged by size:
3700 1557 780 202
PspOMI G'GGCC_C
Cuts at: 249 822 961 1247 1815 2465 249
Size: 573 139 286 568 650 4023
Fragments arranged by size:
4023 650 573 568 286 139
PstI C_TGCA'G
Cuts at: 354 1835 2138 2447 3074 3086 3158 3286 3971
Size: 1481 303 309 627 12 72 128 685
Cuts at: 3971 354
Size: 2622
Fragments arranged by size:
2622 1481 685 627 309 303 128 72
12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6239
PvuII CAG'CTG
Cuts at: 1014 1025 2046 2139 2526 3207 3664 4024 1014
Size: 11 1021 93 387 681 457 360 3229
Fragments arranged by size:
3229 1021 681 457 387 360 93 11
RsrII CG'GwC_CG
Cuts at: 4434 4434
Size: 6239
SacI G_AGCT'C
Cuts at: 614 3253 614
Size: 2639 3600
SacII CC_GC'GG
Cuts at: 2067 2067
Size: 6239
SalI G'TCGA_C
Cuts at: 3276 3276
Size: 6239
SanDI GG'GwC_CC
Cuts at: 539 2096 2876 539
Size: 1557 780 3902
Fragments arranged by size:
3902 1557 780
SapI GCTCTTCn'nnn_
Cuts at: 376 4262 4472 376
Size: 3886 210 2143
Fragments arranged by size:
3886 2143 210
SbfI CC_TGCA'GG
Cuts at: 3286 3286
Size: 6239
ScaI AGT'ACT
Cuts at: 5756 5756
Size: 6239
SfcI C'TryA_G
Cuts at: 33 350 726 1524 1831 2134 2443 3070 3082
Size: 317 376 798 307 303 309 627 12
Cuts at: 3082 3154 3282 3967 4935 5126 33
Size: 72 128 685 968 191 1146
Fragments arranged by size:
1146 968 798 685 627 376 317 309
307 303 191 128 72 12
SfoI GGC'GCC
Cuts at: 1007 1798 1824 1871 1973 2360 2792 3671 3919
Size: 791 26 47 102 387 432 879 248
Cuts at: 3919 1007
Size: 3327
Fragments arranged by size:
3327 879 791 432 387 248 102 47
26
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6239
SmaI CCC'GGG
Cuts at: 1875 2280 3261 1875
Size: 405 981 4853
Fragments arranged by size:
4853 981 405
SmlI C'TyrA_G
Cuts at: 439 3394 4309 5023 5285 439
Size: 2955 915 714 262 1393
Fragments arranged by size:
2955 1393 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6239
SphI G_CATG'C
Cuts at: 3292 3792 3292
Size: 500 5739
StuI AGG'CCT
Cuts at: 635 635
Size: 6239
StyI C'CwwG_G
Cuts at: 982 1052 1685 2363 3373 3727 982
Size: 70 633 678 1010 354 3494
Fragments arranged by size:
3494 1010 678 633 354 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 628 739 1065 1561 1777 3231 3822 3955 4414
Size: 111 326 496 216 1454 591 133 459
Cuts at: 4414 4623 628
Size: 209 2244
Fragments arranged by size:
2244 1454 591 496 459 326 216 209
133 111
TatI w'GTAC_w
Cuts at: 1121 1157 2429 5754 1121
Size: 36 1272 3325 1606
Fragments arranged by size:
3325 1606 1272 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 912 944 2875 2907 3037 3069 3173 3205 5756
Size: 32 1931 32 130 32 104 32 2551
Cuts at: 5756 5788 912
Size: 32 1363
Fragments arranged by size:
2551 1931 1363 130 104 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4036 4036
Size: 6239
XbaI T'CTAG_A
Cuts at: 3270 3270
Size: 6239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1527 1749 3225 3734 1527
Size: 222 1476 509 4032
Fragments arranged by size:
4032 1476 509 222
XmaI C'CCGG_G
Cuts at: 1873 2278 3259 1873
Size: 405 981 4853
Fragments arranged by size:
4853 981 405
XmnI GAAnn'nnTTC
Cuts at: 799 799
Size: 6239
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI AvrII BaeI
BbsI BfrBI BmtI BsiWI BsmI BstZ17I ClaI EcoRV
FspAI HindIII HpaI NdeI NheI NotI NsiI PacI
PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI