Restriction map for
pF1KB4877
(Circular) (Six-base) MAPSORT of: pF1KB4877.seq Check: 1735 from: 1 to: 5531
pF1KB4877 5531 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1468 2569 1468
Size: 1101 4430
Acc65I G'GTAC_C
Cuts at: 2547 2547
Size: 5531
AclI AA'CG_TT
Cuts at: 5371 5371
Size: 5531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1250 1481 1544 2005 2391 3356 3788 4141
Size: 1090 231 63 461 386 965 432 353
Cuts at: 4141 4803 160
Size: 662 888
Fragments arranged by size:
1090 965 888 662 461 432 386 353
231 63
AflIII A'CryG_T
Cuts at: 1270 2883 4683 4964 1270
Size: 1613 1800 281 1837
Fragments arranged by size:
1837 1800 1613 281
AleI CACnn'nnGTG
Cuts at: 1406 1406
Size: 5531
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1931 1963 1931
Size: 32 5499
AlwNI CAG_nnn'CTG
Cuts at: 1573 1696 2836 4274 1573
Size: 123 1140 1438 2830
Fragments arranged by size:
2830 1438 1140 123
ApaI G_GGCC'C
Cuts at: 466 466
Size: 5531
ApaLI G'TGCA_C
Cuts at: 4369 4369
Size: 5531
ApoI r'AATT_y
Cuts at: 1177 1417 1744 1776 2202 2535 4884 5140 1177
Size: 240 327 32 426 333 2349 256 1568
Fragments arranged by size:
2349 1568 426 333 327 256 240 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 5531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
AvaI C'yCGr_G
Cuts at: 271 315 328 459 541 709 2551 271
Size: 44 13 131 82 168 1842 3251
Fragments arranged by size:
3251 1842 168 131 82 44 13
BamHI G'GATC_C
Cuts at: 2556 2556
Size: 5531
BanI G'GyrC_C
Cuts at: 702 2547 2961 3209 3244 702
Size: 1845 414 248 35 2989
Fragments arranged by size:
2989 1845 414 248 35
BanII G_rGCy'C
Cuts at: 167 209 272 466 542 1728 2220 2545 3575
Size: 42 63 194 76 1186 492 325 1030
Cuts at: 3575 167
Size: 2123
Fragments arranged by size:
2123 1186 1030 492 325 194 76 63
42
BbeI G_GCGC'C
Cuts at: 706 2965 3213 706
Size: 2259 248 3024
Fragments arranged by size:
3024 2259 248
BbsI GAAGACnn'nnnn_
Cuts at: 500 1889 500
Size: 1389 4142
BbvCI CC'TCA_GC
Cuts at: 866 866
Size: 5531
BciVI GTATCCnnnnn_n'
Cuts at: 3429 4485 5531 3429
Size: 1056 1046 3429
Fragments arranged by size:
3429 1056 1046
BclI T'GATC_A
Cuts at: 3051 3051
Size: 5531
BglI GCCn_nnn'nGGC
Cuts at: 448 652 448
Size: 204 5327
BglII A'GATC_T
Cuts at: 1329 3046 5083 1329
Size: 1717 2037 1777
Fragments arranged by size:
2037 1777 1717
BlpI GC'TnA_GC
Cuts at: 2643 2643
Size: 5531
Bme1580I G_kGCm'C
Cuts at: 466 581 2319 3156 3249 4373 466
Size: 115 1738 837 93 1124 1624
Fragments arranged by size:
1738 1624 1124 837 115 93
BmgBI CAC'GTC
Cuts at: 2176 2176
Size: 5531
BmrI ACTGGGnnnn_n'
Cuts at: 1725 2743 2824 3159 5303 1725
Size: 1018 81 335 2144 1953
Fragments arranged by size:
2144 1953 1018 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 343 504 982 3898 343
Size: 161 478 2916 1976
Fragments arranged by size:
2916 1976 478 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 1034 205
Size: 829 4702
Bpu10I CC'TnA_GC
Cuts at: 866 866
Size: 5531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 181 2707 3586 4300 4598 151
Size: 30 2526 879 714 298 1084
Fragments arranged by size:
2526 1084 879 714 298 30
BsaI GGTCTCn'nnnn_
Cuts at: 199 5235 199
Size: 5036 495
BsaAI yAC'GTr
Cuts at: 2884 2884
Size: 5531
BsaBI GATnn'nnATC
Cuts at: 6 3045 5082 6
Size: 3039 2037 455
Fragments arranged by size:
3039 2037 455
BsaHI Gr'CG_yC
Cuts at: 177 703 2962 3210 3912 5408 177
Size: 526 2259 248 702 1496 300
Fragments arranged by size:
2259 1496 702 526 300 248
BsaWI w'CCGG_w
Cuts at: 2720 3241 3919 4330 4477 4751 2720
Size: 521 678 411 147 274 3500
Fragments arranged by size:
3500 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 256 376 379 394 397 445 487 490
Size: 81 120 3 15 3 48 42 3
Cuts at: 490 493 854 1833 2225 2228 175
Size: 3 361 979 392 3 3478
Fragments arranged by size:
3478 979 392 361 120 81 48 42
15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 307 2956 3714 4379 4787 307
Size: 2649 758 665 408 1051
Fragments arranged by size:
2649 1051 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 1430
Size: 5531
BsiEI CG_ry'CG
Cuts at: 104 347 455 3073 3119 4349 104
Size: 243 108 2618 46 1230 1286
Fragments arranged by size:
2618 1286 1230 243 108 46
BsiHKAI G_wGCw'C
Cuts at: 167 209 542 2545 3323 3513 4373 167
Size: 42 333 2003 778 190 860 1325
Fragments arranged by size:
2003 1325 860 778 333 190 42
BsmI GAATG_Cn'
Cuts at: 2381 2381
Size: 5531
BsmBI CGTCTCn'nnnn_
Cuts at: 970 970
Size: 5531
Bsp1286I G_dGCh'C
Cuts at: 167 209 272 466 542 581 1728 2220 2319
Size: 42 63 194 76 39 1147 492 99
Cuts at: 2319 2545 3156 3249 3323 3513 3575 4373 167
Size: 226 611 93 74 190 62 798 1325
Fragments arranged by size:
1325 1147 798 611 492 226 194 190
99 93 76 74 63 62 42 39
BspHI T'CATG_A
Cuts at: 3963 3963
Size: 5531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1660 2581 2795 3097 3478 1660
Size: 921 214 302 381 3713
Fragments arranged by size:
3713 921 381 302 214
BsrBI CCG'CTC
Cuts at: 50 400 3823 3877 5361 5526 50
Size: 350 3423 54 1484 165 55
Fragments arranged by size:
3423 1484 350 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3443 3443
Size: 5531
BsrFI r'CCGG_y
Cuts at: 346 603 3529 3710 346
Size: 257 2926 181 2167
Fragments arranged by size:
2926 2167 257 181
BssSI C'ACGA_G
Cuts at: 797 2312 3802 4510 797
Size: 1515 1490 708 1818
Fragments arranged by size:
1818 1515 1490 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2908 2908
Size: 5531
BstBI TT'CG_AA
Cuts at: 3892 3892
Size: 5531
BstXI CCAn_nnnn'nTGG
Cuts at: 1738 1738
Size: 5531
BstYI r'GATC_y
Cuts at: 1 1329 2556 3023 3046 3381 3627 3933 3945
Size: 1328 1227 467 23 335 246 306 12
Cuts at: 3945 4031 5083 1
Size: 86 1052 449
Fragments arranged by size:
1328 1227 1052 467 449 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1469 1469
Size: 5531
BtgI C'CryG_G
Cuts at: 263 341 443 656 813 2334 2494 263
Size: 78 102 213 157 1521 160 3300
Fragments arranged by size:
3300 1521 213 160 157 102 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 368 3481 3662 3825 368
Size: 3113 181 163 2074
Fragments arranged by size:
3113 2074 181 163
BtsI GCAGTG_nn'
Cuts at: 2774 2774
Size: 5531
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2175 2210 2352 2387 2175
Size: 35 142 35 5319
Fragments arranged by size:
5319 142 35 35
DraI TTT'AAA
Cuts at: 948 2530 948
Size: 1582 3949
DrdI GACnn_nn'nnGTC
Cuts at: 1800 3237 4581 1800
Size: 1437 1344 2750
Fragments arranged by size:
2750 1437 1344
EaeI y'GGCC_r
Cuts at: 344 452 810 1375 2163 2331 3116 3290 3681
Size: 108 358 565 788 168 785 174 391
Cuts at: 3681 3708 5053 344
Size: 27 1345 822
Fragments arranged by size:
1345 822 788 785 565 391 358 174
168 108 27
EagI C'GGCC_G
Cuts at: 344 452 3116 344
Size: 108 2664 2759
Fragments arranged by size:
2759 2664 108
EarI CTCTTCn'nnn_
Cuts at: 353 365 371 545 658 830 1014 1457 1520
Size: 12 6 174 113 172 184 443 63
Cuts at: 1520 2084 3554 3764 353
Size: 564 1470 210 2120
Fragments arranged by size:
2120 1470 564 443 210 184 174 172
113 63 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 782 2249 3739 4481 4627 5341 782
Size: 1467 1490 742 146 714 972
Fragments arranged by size:
1490 1467 972 742 714 146
EcoICRI GAG'CTC
Cuts at: 165 207 540 2543 165
Size: 42 333 2003 3153
Fragments arranged by size:
3153 2003 333 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 205 1034 1250 1481 1544 2005 2391 3356
Size: 45 829 216 231 63 461 386 965
Cuts at: 3356 3788 4141 4803 160
Size: 432 353 662 888
Fragments arranged by size:
965 888 829 662 461 432 386 353
231 216 63 45
EcoNI CCTnn'n_nnAGG
Cuts at: 331 1978 331
Size: 1647 3884
EcoO109I rG'GnC_Cy
Cuts at: 1519 2670 1519
Size: 1151 4380
EcoRI G'AATT_C
Cuts at: 2535 2535
Size: 5531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3550 3550
Size: 5531
FspI TGC'GCA
Cuts at: 3312 3312
Size: 5531
HaeII r_GCGC'y
Cuts at: 315 706 2965 3213 4443 5214 315
Size: 391 2259 248 1230 771 632
Fragments arranged by size:
2259 1230 771 632 391 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3373 3403 3405 3435 3373
Size: 30 2 30 5469
Fragments arranged by size:
5469 30 30 2
HincII GTy'rAC
Cuts at: 2570 2570
Size: 5531
KasI G'GCGC_C
Cuts at: 702 2961 3209 702
Size: 2259 248 3024
Fragments arranged by size:
3024 2259 248
KpnI G_GTAC'C
Cuts at: 2551 2551
Size: 5531
MluI A'CGCG_T
Cuts at: 4964 4964
Size: 5531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 128 4290 4474 128
Size: 4162 184 1185
Fragments arranged by size:
4162 1185 184
MscI TGG'CCA
Cuts at: 2165 3292 2165
Size: 1127 4404
MslI CAynn'nnrTG
Cuts at: 812 899 1406 3520 3647 812
Size: 87 507 2114 127 2696
Fragments arranged by size:
2696 2114 507 127 87
MspA1I CmG'CkG
Cuts at: 307 343 445 658 671 2642 2956 3316 4100
Size: 36 102 213 13 1971 314 360 784
Cuts at: 4100 4345 307
Size: 245 1493
Fragments arranged by size:
1971 1493 784 360 314 245 213 102
36 13
NaeI GCC'GGC
Cuts at: 348 605 3712 348
Size: 257 3107 2167
Fragments arranged by size:
3107 2167 257
NarI GG'CG_CC
Cuts at: 703 2962 3210 703
Size: 2259 248 3024
Fragments arranged by size:
3024 2259 248
NcoI C'CATG_G
Cuts at: 263 2494 263
Size: 2231 3300
NgoMIV G'CCGG_C
Cuts at: 346 603 3710 346
Size: 257 3107 2167
Fragments arranged by size:
3107 2167 257
NspI r_CATG'y
Cuts at: 1217 1274 1393 1603 1945 2259 2584 3084 4687
Size: 57 119 210 342 314 325 500 1603
Cuts at: 4687 1217
Size: 2061
Fragments arranged by size:
2061 1603 500 342 325 314 210 119
57
PasI CC'CwG_GG
Cuts at: 2221 2221
Size: 5531
PciI A'CATG_T
Cuts at: 1270 4683 1270
Size: 3413 2118
PflMI CCAn_nnn'nTGG
Cuts at: 1573 1573
Size: 5531
PfoI T'CCnGG_A
Cuts at: 521 521
Size: 5531
PmeI GTTT'AAAC
Cuts at: 2530 2530
Size: 5531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2124 3977 4009 2092
Size: 32 1853 32 3614
Fragments arranged by size:
3614 1853 32 32
PshAI GACnn'nnGTC
Cuts at: 191 191
Size: 5531
PsiI TTA'TAA
Cuts at: 929 929
Size: 5531
PspOMI G'GGCC_C
Cuts at: 462 462
Size: 5531
PspXI vC'TCGA_Gb
Cuts at: 328 328
Size: 5531
PstI C_TGCA'G
Cuts at: 2578 3263 2578
Size: 685 4846
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5531
PvuII CAG'CTG
Cuts at: 2956 3316 2956
Size: 360 5171
RsrII CG'GwC_CG
Cuts at: 3726 3726
Size: 5531
SacI G_AGCT'C
Cuts at: 167 209 542 2545 167
Size: 42 333 2003 3153
Fragments arranged by size:
3153 2003 333 42
SacII CC_GC'GG
Cuts at: 344 446 659 344
Size: 102 213 5216
Fragments arranged by size:
5216 213 102
SalI G'TCGA_C
Cuts at: 2568 2568
Size: 5531
SapI GCTCTTCn'nnn_
Cuts at: 658 1014 2084 3554 3764 658
Size: 356 1070 1470 210 2425
Fragments arranged by size:
2425 1470 1070 356 210
SbfI CC_TGCA'GG
Cuts at: 2578 2578
Size: 5531
ScaI AGT'ACT
Cuts at: 5048 5048
Size: 5531
SfcI C'TryA_G
Cuts at: 33 2574 3259 4227 4418 33
Size: 2541 685 968 191 1146
Fragments arranged by size:
2541 1146 968 685 191
SfoI GGC'GCC
Cuts at: 704 2963 3211 704
Size: 2259 248 3024
Fragments arranged by size:
3024 2259 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
SgrAI Cr'CCGG_yG
Cuts at: 603 603
Size: 5531
SmaI CCC'GGG
Cuts at: 273 711 2553 273
Size: 438 1842 3251
Fragments arranged by size:
3251 1842 438
SmlI C'TyrA_G
Cuts at: 166 196 328 2686 3601 4315 4577 166
Size: 30 132 2358 915 714 262 1120
Fragments arranged by size:
2358 1120 915 714 262 132 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5531
SphI G_CATG'C
Cuts at: 2584 3084 2584
Size: 500 5031
StuI AGG'CCT
Cuts at: 326 326
Size: 5531
StyI C'CwwG_G
Cuts at: 263 647 2494 2514 2665 3019 263
Size: 384 1847 20 151 354 2775
Fragments arranged by size:
2775 1847 384 354 151 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3114 3247 3706 3915 3114
Size: 133 459 209 4730
Fragments arranged by size:
4730 459 209 133
TatI w'GTAC_w
Cuts at: 827 5046 827
Size: 4219 1312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5048 5080 5048
Size: 32 5499
Tth111I GACn'n_nGTC
Cuts at: 3328 3328
Size: 5531
XbaI T'CTAG_A
Cuts at: 2562 2562
Size: 5531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2173 3026 2173
Size: 853 4678
XhoI C'TCGA_G
Cuts at: 328 328
Size: 5531
XmaI C'CCGG_G
Cuts at: 271 709 2551 271
Size: 438 1842 3251
Fragments arranged by size:
3251 1842 438
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI BspEI
BsrGI BssHII BstEII Bsu36I ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI SwaI XmnI ZraI