Restriction map for
pF1KB4879
(Circular) (Six-base) MAPSORT of: pF1KB4879.seq Check: 8407 from: 1 to: 5543
pF1KB4879 5543 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1800 1800
Size: 5543
AatII G_ACGT'C
Cuts at: 1059 2210 1059
Size: 1151 4392
AccI GT'mk_AC
Cuts at: 2581 2581
Size: 5543
Acc65I G'GTAC_C
Cuts at: 2559 2559
Size: 5543
AclI AA'CG_TT
Cuts at: 5383 5383
Size: 5543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1012 1441 1910 3368 3800 4153 4815 257
Size: 755 429 469 1458 432 353 662 985
Fragments arranged by size:
1458 985 755 662 469 432 429 353
AflIII A'CryG_T
Cuts at: 1559 2386 2486 2895 4695 4976 1559
Size: 827 100 409 1800 281 2126
Fragments arranged by size:
2126 1800 827 409 281 100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1685 1685
Size: 5543
AlwNI CAG_nnn'CTG
Cuts at: 235 264 511 1750 2848 4286 235
Size: 29 247 1239 1098 1438 1492
Fragments arranged by size:
1492 1438 1239 1098 247 29
ApaLI G'TGCA_C
Cuts at: 4381 4381
Size: 5543
ApoI r'AATT_y
Cuts at: 144 457 948 1603 1624 2547 4896 5152 144
Size: 313 491 655 21 923 2349 256 535
Fragments arranged by size:
2349 923 655 535 491 313 256 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 5543
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5543
AvaI C'yCGr_G
Cuts at: 2563 2563
Size: 5543
AvrII C'CTAG_G
Cuts at: 1344 1344
Size: 5543
BamHI G'GATC_C
Cuts at: 1618 2568 1618
Size: 950 4593
BanI G'GyrC_C
Cuts at: 2559 2973 3221 3256 2559
Size: 414 248 35 4846
Fragments arranged by size:
4846 414 248 35
BanII G_rGCy'C
Cuts at: 1937 2557 3587 1937
Size: 620 1030 3893
Fragments arranged by size:
3893 1030 620
BbeI G_GCGC'C
Cuts at: 2977 3225 2977
Size: 248 5295
BbsI GAAGACnn'nnnn_
Cuts at: 305 857 1007 1018 1910 2149 305
Size: 552 150 11 892 239 3699
Fragments arranged by size:
3699 892 552 239 150 11
BbvCI CC'TCA_GC
Cuts at: 206 206
Size: 5543
BciVI GTATCCnnnnn_n'
Cuts at: 3441 4497 5543 3441
Size: 1056 1046 3441
Fragments arranged by size:
3441 1056 1046
BclI T'GATC_A
Cuts at: 2408 2436 3063 2408
Size: 28 627 4888
Fragments arranged by size:
4888 627 28
BglII A'GATC_T
Cuts at: 3058 5095 3058
Size: 2037 3506
BlpI GC'TnA_GC
Cuts at: 1925 2655 1925
Size: 730 4813
Bme1580I G_kGCm'C
Cuts at: 3168 3261 4385 3168
Size: 93 1124 4326
Fragments arranged by size:
4326 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1126 1505 2755 2836 3171 5315 1126
Size: 379 1250 81 335 2144 1354
Fragments arranged by size:
2144 1354 1250 379 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3910 3910
Size: 5543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 1487 1226
Size: 261 5282
Bpu10I CC'TnA_GC
Cuts at: 206 581 797 1080 1247 1916 206
Size: 375 216 283 167 669 3833
Fragments arranged by size:
3833 669 375 283 216 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1631 2719 3598 4312 4610 1288
Size: 343 1088 879 714 298 2221
Fragments arranged by size:
2221 1088 879 714 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 2221 2317 2512 5247 2221
Size: 96 195 2735 2517
Fragments arranged by size:
2735 2517 195 96
BsaAI yAC'GTr
Cuts at: 2389 2896 2389
Size: 507 5036
BsaBI GATnn'nnATC
Cuts at: 6 1575 3057 5094 6
Size: 1569 1482 2037 455
Fragments arranged by size:
2037 1569 1482 455
BsaHI Gr'CG_yC
Cuts at: 1056 1723 1826 2207 2974 3222 3924 5420 1056
Size: 667 103 381 767 248 702 1496 1179
Fragments arranged by size:
1496 1179 767 702 667 381 248 103
BsaWI w'CCGG_w
Cuts at: 516 2732 3253 3931 4342 4489 4763 516
Size: 2216 521 678 411 147 274 1296
Fragments arranged by size:
2216 1296 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 348 1987 348
Size: 1639 3904
BseYI C'CCAG_C
Cuts at: 984 2968 3726 4391 4799 984
Size: 1984 758 665 408 1728
Fragments arranged by size:
1984 1728 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1772 584
Size: 1188 4355
BsiEI CG_ry'CG
Cuts at: 104 3085 3131 4361 104
Size: 2981 46 1230 1286
Fragments arranged by size:
2981 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 646 1674 2557 3335 3525 4385 646
Size: 1028 883 778 190 860 1804
Fragments arranged by size:
1804 1028 883 860 778 190
BsmI GAATG_Cn'
Cuts at: 1278 1278
Size: 5543
Bsp1286I G_dGCh'C
Cuts at: 646 1674 1937 2557 3168 3261 3335 3525 3587
Size: 1028 263 620 611 93 74 190 62
Cuts at: 3587 4385 646
Size: 798 1804
Fragments arranged by size:
1804 1028 798 620 611 263 190 93
74 62
BspHI T'CATG_A
Cuts at: 3975 3975
Size: 5543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 918 1220 1800 2593 2807 3109 3490 918
Size: 302 580 793 214 302 381 2971
Fragments arranged by size:
2971 793 580 381 302 302 214
BsrBI CCG'CTC
Cuts at: 50 321 3835 3889 5373 5538 50
Size: 271 3514 54 1484 165 55
Fragments arranged by size:
3514 1484 271 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3455 3455
Size: 5543
BsrFI r'CCGG_y
Cuts at: 3541 3722 3541
Size: 181 5362
BsrGI T'GTAC_A
Cuts at: 2448 2448
Size: 5543
BssSI C'ACGA_G
Cuts at: 3814 4522 3814
Size: 708 4835
BstAPI GCAn_nnn'nTGC
Cuts at: 2920 2920
Size: 5543
BstBI TT'CG_AA
Cuts at: 3904 3904
Size: 5543
BstYI r'GATC_y
Cuts at: 1 543 600 1618 2568 3035 3058 3393 3639
Size: 542 57 1018 950 467 23 335 246
Cuts at: 3639 3945 3957 4043 5095 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 1018 950 542 467 449 335 306
246 86 57 23 12
BtgI C'CryG_G
Cuts at: 1140 2041 1140
Size: 901 4642
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3493 3674 3837 3493
Size: 181 163 5199
Fragments arranged by size:
5199 181 163
BtsI GCAGTG_nn'
Cuts at: 567 738 2786 567
Size: 171 2048 3324
Fragments arranged by size:
3324 2048 171
DraI TTT'AAA
Cuts at: 491 1167 2542 491
Size: 676 1375 3492
Fragments arranged by size:
3492 1375 676
DrdI GACnn_nn'nnGTC
Cuts at: 2290 3249 4593 2290
Size: 959 1344 3240
Fragments arranged by size:
3240 1344 959
EaeI y'GGCC_r
Cuts at: 1638 3128 3302 3693 3720 5065 1638
Size: 1490 174 391 27 1345 2116
Fragments arranged by size:
2116 1490 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3128 3128
Size: 5543
EarI CTCTTCn'nnn_
Cuts at: 115 161 473 632 1193 3566 3776 115
Size: 46 312 159 561 2373 210 1882
Fragments arranged by size:
2373 1882 561 312 210 159 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 897 3751 4493 4639 5353 897
Size: 2854 742 146 714 1087
Fragments arranged by size:
2854 1087 742 714 146
EcoICRI GAG'CTC
Cuts at: 2555 2555
Size: 5543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 1012 1226 1441 1487 1910 3368 3800 4153
Size: 755 214 215 46 423 1458 432 353
Cuts at: 4153 4815 257
Size: 662 985
Fragments arranged by size:
1458 985 755 662 432 423 353 215
214 46
EcoO109I rG'GnC_Cy
Cuts at: 495 2128 2442 2682 495
Size: 1633 314 240 3356
Fragments arranged by size:
3356 1633 314 240
EcoRI G'AATT_C
Cuts at: 2547 2547
Size: 5543
EcoRV GAT'ATC
Cuts at: 1338 1338
Size: 5543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 829 3562 829
Size: 2733 2810
FspI TGC'GCA
Cuts at: 3324 3324
Size: 5543
HaeII r_GCGC'y
Cuts at: 2977 3225 4455 5226 2977
Size: 248 1230 771 3294
Fragments arranged by size:
3294 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 1230 1262 1322 1354 1635 1667 3385
Size: 32 888 32 60 32 281 32 1718
Cuts at: 3385 3415 3417 3447 310
Size: 30 2 30 2406
Fragments arranged by size:
2406 1718 888 281 60 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 995 2582 995
Size: 1587 3956
HindIII A'AGCT_T
Cuts at: 269 701 269
Size: 432 5111
HpaI GTT'AAC
Cuts at: 995 995
Size: 5543
KasI G'GCGC_C
Cuts at: 2973 3221 2973
Size: 248 5295
KpnI G_GTAC'C
Cuts at: 2563 2563
Size: 5543
MfeI C'AATT_G
Cuts at: 500 500
Size: 5543
MluI A'CGCG_T
Cuts at: 4976 4976
Size: 5543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 737 4302 4486 737
Size: 3565 184 1794
Fragments arranged by size:
3565 1794 184
MscI TGG'CCA
Cuts at: 3304 3304
Size: 5543
MslI CAynn'nnrTG
Cuts at: 2491 3532 3659 2491
Size: 1041 127 4375
Fragments arranged by size:
4375 1041 127
MspA1I CmG'CkG
Cuts at: 235 1750 2654 2968 3328 4112 4357 235
Size: 1515 904 314 360 784 245 1421
Fragments arranged by size:
1515 1421 904 784 360 314 245
NaeI GCC'GGC
Cuts at: 3724 3724
Size: 5543
NarI GG'CG_CC
Cuts at: 2974 3222 2974
Size: 248 5295
NcoI C'CATG_G
Cuts at: 1140 1140
Size: 5543
NgoMIV G'CCGG_C
Cuts at: 3722 3722
Size: 5543
NspI r_CATG'y
Cuts at: 738 796 2490 2596 3096 4699 738
Size: 58 1694 106 500 1603 1582
Fragments arranged by size:
1694 1603 1582 500 106 58
PciI A'CATG_T
Cuts at: 2486 4695 2486
Size: 2209 3334
PflMI CCAn_nnn'nTGG
Cuts at: 1146 1146
Size: 5543
PfoI T'CCnGG_A
Cuts at: 1726 1726
Size: 5543
PmeI GTTT'AAAC
Cuts at: 2542 2542
Size: 5543
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 506 538 3989 4021 506
Size: 32 3451 32 2028
Fragments arranged by size:
3451 2028 32 32
PpuMI rG'GwC_Cy
Cuts at: 2128 2442 2128
Size: 314 5229
PshAI GACnn'nnGTC
Cuts at: 1769 1964 1769
Size: 195 5348
PsiI TTA'TAA
Cuts at: 1433 1433
Size: 5543
PstI C_TGCA'G
Cuts at: 2590 3275 2590
Size: 685 4858
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5543
PvuII CAG'CTG
Cuts at: 235 1750 2968 3328 235
Size: 1515 1218 360 2450
Fragments arranged by size:
2450 1515 1218 360
RsrII CG'GwC_CG
Cuts at: 3738 3738
Size: 5543
SacI G_AGCT'C
Cuts at: 2557 2557
Size: 5543
SalI G'TCGA_C
Cuts at: 2580 2580
Size: 5543
SapI GCTCTTCn'nnn_
Cuts at: 3566 3776 3566
Size: 210 5333
SbfI CC_TGCA'GG
Cuts at: 2590 2590
Size: 5543
ScaI AGT'ACT
Cuts at: 5060 5060
Size: 5543
SfcI C'TryA_G
Cuts at: 33 1580 2586 3271 4239 4430 33
Size: 1547 1006 685 968 191 1146
Fragments arranged by size:
1547 1146 1006 968 685 191
SfoI GGC'GCC
Cuts at: 2975 3223 2975
Size: 248 5295
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5543
SmaI CCC'GGG
Cuts at: 2565 2565
Size: 5543
SmlI C'TyrA_G
Cuts at: 1267 1646 2698 3613 4327 4589 1267
Size: 379 1052 915 714 262 2221
Fragments arranged by size:
2221 1052 915 714 379 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5543
SphI G_CATG'C
Cuts at: 2596 3096 2596
Size: 500 5043
StuI AGG'CCT
Cuts at: 1686 1686
Size: 5543
StyI C'CwwG_G
Cuts at: 943 1140 1187 1344 1405 2498 2677 3031 943
Size: 197 47 157 61 1093 179 354 3455
Fragments arranged by size:
3455 1093 354 197 179 157 61 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1781 2495 2536 3126 3259 3718 3927 1781
Size: 714 41 590 133 459 209 3397
Fragments arranged by size:
3397 714 590 459 209 133 41
TatI w'GTAC_w
Cuts at: 2448 5058 2448
Size: 2610 2933
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1530 1562 5060 5092 1530
Size: 32 3498 32 1981
Fragments arranged by size:
3498 1981 32 32
Tth111I GACn'n_nGTC
Cuts at: 3340 3340
Size: 5543
XbaI T'CTAG_A
Cuts at: 2574 2574
Size: 5543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3038 3038
Size: 5543
XmaI C'CCGG_G
Cuts at: 2563 2563
Size: 5543
ZraI GAC'GTC
Cuts at: 1057 2208 1057
Size: 1151 4392
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AloI ApaI AscI
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI NdeI NheI NotI
NruI NsiI PacI PasI PmlI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI