Restriction map for
pF1KB4886
(Circular) (Six-base) MAPSORT of: pF1KB4886.seq Check: 8576 from: 1 to: 4133
pF1KB4886 4133 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1171 1171
Size: 4133
Acc65I G'GTAC_C
Cuts at: 539 1149 539
Size: 610 3523
AclI AA'CG_TT
Cuts at: 1127 3973 1127
Size: 2846 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1958 2390 3137 3405 1958
Size: 432 747 268 2686
Fragments arranged by size:
2686 747 432 268
AflIII A'CryG_T
Cuts at: 1485 2589 3566 1485
Size: 1104 977 2052
Fragments arranged by size:
2052 1104 977
AgeI A'CCGG_T
Cuts at: 2521 2521
Size: 4133
AleI CACnn'nnGTG
Cuts at: 703 703
Size: 4133
AlwNI CAG_nnn'CTG
Cuts at: 1438 3005 1438
Size: 1567 2566
ApaLI G'TGCA_C
Cuts at: 2903 2903
Size: 4133
ApoI r'AATT_y
Cuts at: 1137 3486 3742 1137
Size: 2349 256 1528
Fragments arranged by size:
2349 1528 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4133
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
AvaI C'yCGr_G
Cuts at: 1060 1153 1060
Size: 93 4040
BamHI G'GATC_C
Cuts at: 1158 1158
Size: 4133
BanI G'GyrC_C
Cuts at: 266 539 712 1149 1563 1811 1846 266
Size: 273 173 437 414 248 35 2553
Fragments arranged by size:
2553 437 414 273 248 173 35
BanII G_rGCy'C
Cuts at: 890 1099 1147 2177 890
Size: 209 48 1030 2846
Fragments arranged by size:
2846 1030 209 48
BbeI G_GCGC'C
Cuts at: 716 1567 1815 716
Size: 851 248 3034
Fragments arranged by size:
3034 851 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 778 812 778
Size: 34 4099
BciVI GTATCCnnnnn_n'
Cuts at: 588 2031 2792 4133 588
Size: 1443 761 1341 588
Fragments arranged by size:
1443 1341 761 588
BclI T'GATC_A
Cuts at: 1653 1653
Size: 4133
BglII A'GATC_T
Cuts at: 278 1118 1648 3685 278
Size: 840 530 2037 726
Fragments arranged by size:
2037 840 726 530
BlpI GC'TnA_GC
Cuts at: 1245 1245
Size: 4133
Bme1580I G_kGCm'C
Cuts at: 269 609 1758 1851 2907 269
Size: 340 1149 93 1056 1495
Fragments arranged by size:
1495 1149 1056 340 93
BmgBI CAC'GTC
Cuts at: 744 744
Size: 4133
BmrI ACTGGGnnnn_n'
Cuts at: 217 303 545 1345 1426 1761 3905 217
Size: 86 242 800 81 335 2144 445
Fragments arranged by size:
2144 800 445 335 242 86 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2500 2500
Size: 4133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 4133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 469 1099 1309 2188 2680 2978 291
Size: 178 630 210 879 492 298 1446
Fragments arranged by size:
1446 879 630 492 298 210 178
BsaI GGTCTCn'nnnn_
Cuts at: 3837 3837
Size: 4133
BsaAI yAC'GTr
Cuts at: 854 1486 854
Size: 632 3501
BsaBI GATnn'nnATC
Cuts at: 6 1647 3684 6
Size: 1641 2037 455
Fragments arranged by size:
2037 1641 455
BsaHI Gr'CG_yC
Cuts at: 713 848 1564 1812 2514 4010 713
Size: 135 716 248 702 1496 836
Fragments arranged by size:
1496 836 716 702 248 135
BsaWI w'CCGG_w
Cuts at: 149 1322 1843 2521 2795 2942 3353 149
Size: 1173 521 678 274 147 411 929
Fragments arranged by size:
1173 929 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 454 484 454
Size: 30 4103
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 387 880 387
Size: 493 3640
BseYI C'CCAG_C
Cuts at: 211 1558 2316 2893 3389 211
Size: 1347 758 577 496 955
Fragments arranged by size:
1347 955 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1040 1040
Size: 4133
BsiEI CG_ry'CG
Cuts at: 104 1017 1675 1721 2929 104
Size: 913 658 46 1208 1308
Fragments arranged by size:
1308 1208 913 658 46
BsiHKAI G_wGCw'C
Cuts at: 890 1147 1925 2115 2907 890
Size: 257 778 190 792 2116
Fragments arranged by size:
2116 792 778 257 190
Bsp1286I G_dGCh'C
Cuts at: 269 609 890 1099 1147 1758 1851 1925 2115
Size: 340 281 209 48 611 93 74 190
Cuts at: 2115 2177 2907 269
Size: 62 730 1495
Fragments arranged by size:
1495 730 611 340 281 209 190 93
74 62 48
BspEI T'CCGG_A
Cuts at: 149 3353 149
Size: 3204 929
BspHI T'CATG_A
Cuts at: 3309 3309
Size: 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 878 1183 1397 1699 2080 878
Size: 305 214 302 381 2931
Fragments arranged by size:
2931 381 305 302 214
BsrBI CCG'CTC
Cuts at: 50 2425 2479 3963 4128 50
Size: 2375 54 1484 165 55
Fragments arranged by size:
2375 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2045 2045
Size: 4133
BsrFI r'CCGG_y
Cuts at: 835 2131 2312 2521 835
Size: 1296 181 209 2447
Fragments arranged by size:
2447 1296 209 181
BssSI C'ACGA_G
Cuts at: 2404 2762 2404
Size: 358 3775
BstAPI GCAn_nnn'nTGC
Cuts at: 1510 1510
Size: 4133
BstBI TT'CG_AA
Cuts at: 2494 2494
Size: 4133
BstEII G'GTnAC_C
Cuts at: 1065 1065
Size: 4133
BstYI r'GATC_y
Cuts at: 1 158 278 1118 1158 1625 1648 1983 2229
Size: 157 120 840 40 467 23 335 246
Cuts at: 2229 3241 3327 3339 3685 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 840 467 449 346 335 246 157
120 86 40 23 12
Bsu36I CC'TnA_GG
Cuts at: 429 429
Size: 4133
BtgI C'CryG_G
Cuts at: 261 1091 261
Size: 830 3303
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2083 2264 2427 2083
Size: 181 163 3789
Fragments arranged by size:
3789 181 163
BtsI GCAGTG_nn'
Cuts at: 806 1376 806
Size: 570 3563
DraI TTT'AAA
Cuts at: 1132 1132
Size: 4133
DrdI GACnn_nn'nnGTC
Cuts at: 1839 2697 1839
Size: 858 3275
EaeI y'GGCC_r
Cuts at: 528 693 777 1035 1088 1718 1892 2283 2310
Size: 165 84 258 53 630 174 391 27
Cuts at: 2310 3655 528
Size: 1345 1006
Fragments arranged by size:
1345 1006 630 391 258 174 165 84
53 27
EagI C'GGCC_G
Cuts at: 1718 1718
Size: 4133
EarI CTCTTCn'nnn_
Cuts at: 868 2156 2366 868
Size: 1288 210 2635
Fragments arranged by size:
2635 1288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2341 2651 2797 3943 2341
Size: 310 146 1146 2531
Fragments arranged by size:
2531 1146 310 146
EcoICRI GAG'CTC
Cuts at: 888 1145 888
Size: 257 3876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1958 2390 3137 3405 139
Size: 1819 432 747 268 867
Fragments arranged by size:
1819 867 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 245 254 1272 245
Size: 9 1018 3106
Fragments arranged by size:
3106 1018 9
EcoRI G'AATT_C
Cuts at: 1137 1137
Size: 4133
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 4133
FspI TGC'GCA
Cuts at: 1914 1914
Size: 4133
HaeII r_GCGC'y
Cuts at: 326 716 1567 1815 2837 3816 326
Size: 390 851 248 1022 979 643
Fragments arranged by size:
1022 979 851 643 390 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 655 687 1975 2005 2007 2037 655
Size: 32 1288 30 2 30 2751
Fragments arranged by size:
2751 1288 32 30 30 2
HincII GTy'rAC
Cuts at: 1172 1172
Size: 4133
KasI G'GCGC_C
Cuts at: 712 1563 1811 712
Size: 851 248 3034
Fragments arranged by size:
3034 851 248
KpnI G_GTAC'C
Cuts at: 543 1153 543
Size: 610 3523
MluI A'CGCG_T
Cuts at: 3566 3566
Size: 4133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 812 2804 2988 437
Size: 375 1992 184 1582
Fragments arranged by size:
1992 1582 375 184
MscI TGG'CCA
Cuts at: 530 1037 1090 1894 530
Size: 507 53 804 2769
Fragments arranged by size:
2769 804 507 53
MslI CAynn'nnrTG
Cuts at: 703 1084 2122 2249 703
Size: 381 1038 127 2587
Fragments arranged by size:
2587 1038 381 127
MspA1I CmG'CkG
Cuts at: 211 390 950 1244 1558 1918 2931 3176 211
Size: 179 560 294 314 360 1013 245 1168
Fragments arranged by size:
1168 1013 560 360 314 294 245 179
NaeI GCC'GGC
Cuts at: 2314 2314
Size: 4133
NarI GG'CG_CC
Cuts at: 713 1564 1812 713
Size: 851 248 3034
Fragments arranged by size:
3034 851 248
NgoMIV G'CCGG_C
Cuts at: 2312 2312
Size: 4133
NspI r_CATG'y
Cuts at: 182 1186 1686 2593 182
Size: 1004 500 907 1722
Fragments arranged by size:
1722 1004 907 500
PciI A'CATG_T
Cuts at: 2589 2589
Size: 4133
PflMI CCAn_nnn'nTGG
Cuts at: 293 293
Size: 4133
PfoI T'CCnGG_A
Cuts at: 161 560 161
Size: 399 3734
PmeI GTTT'AAAC
Cuts at: 1132 1132
Size: 4133
PmlI CAC'GTG
Cuts at: 854 854
Size: 4133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 4101
PpuMI rG'GwC_Cy
Cuts at: 245 254 245
Size: 9 4124
PstI C_TGCA'G
Cuts at: 1180 1865 1180
Size: 685 3448
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4133
PvuII CAG'CTG
Cuts at: 211 1558 1918 211
Size: 1347 360 2426
Fragments arranged by size:
2426 1347 360
RsrII CG'GwC_CG
Cuts at: 2328 2328
Size: 4133
SacI G_AGCT'C
Cuts at: 890 1147 890
Size: 257 3876
SalI G'TCGA_C
Cuts at: 1170 1170
Size: 4133
SapI GCTCTTCn'nnn_
Cuts at: 2156 2366 2156
Size: 210 3923
SbfI CC_TGCA'GG
Cuts at: 1180 1180
Size: 4133
ScaI AGT'ACT
Cuts at: 3650 3650
Size: 4133
SfcI C'TryA_G
Cuts at: 33 477 1176 1861 2854 3045 33
Size: 444 699 685 993 191 1121
Fragments arranged by size:
1121 993 699 685 444 191
SfoI GGC'GCC
Cuts at: 714 1565 1813 714
Size: 851 248 3034
Fragments arranged by size:
3034 851 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4133
SmaI CCC'GGG
Cuts at: 1155 1155
Size: 4133
SmlI C'TyrA_G
Cuts at: 306 484 1114 1288 2203 2695 2957 306
Size: 178 630 174 915 492 262 1482
Fragments arranged by size:
1482 915 630 492 262 178 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4133
SphI G_CATG'C
Cuts at: 1186 1686 1186
Size: 500 3633
StyI C'CwwG_G
Cuts at: 1267 1621 1267
Size: 354 3779
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 1716 1849 2308 2517 316
Size: 1400 133 459 209 1932
Fragments arranged by size:
1932 1400 459 209 133
TatI w'GTAC_w
Cuts at: 896 3648 896
Size: 2752 1381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 246 278 742 774 3650 3682 246
Size: 32 464 32 2876 32 697
Fragments arranged by size:
2876 697 464 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1930 1930
Size: 4133
XbaI T'CTAG_A
Cuts at: 1164 1164
Size: 4133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 208 1628 114
Size: 94 1420 2619
Fragments arranged by size:
2619 1420 94
XmaI C'CCGG_G
Cuts at: 1153 1153
Size: 4133
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI