Restriction map for
pF1KB4895
(Circular) (Six-base) MAPSORT of: pF1KB4895.seq Check: 7149 from: 1 to: 4460
pF1KB4895 4460 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1498 1498
Size: 4460
Acc65I G'GTAC_C
Cuts at: 1476 1476
Size: 4460
AclI AA'CG_TT
Cuts at: 4300 4300
Size: 4460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 695 2285 2717 3070 3732 317
Size: 378 1590 432 353 662 1045
Fragments arranged by size:
1590 1045 662 432 378 353
AflIII A'CryG_T
Cuts at: 1812 3612 3893 1812
Size: 1800 281 2379
Fragments arranged by size:
2379 1800 281
AgeI A'CCGG_T
Cuts at: 1090 1090
Size: 4460
AhdI GACnn_n'nnGTC
Cuts at: 1440 1440
Size: 4460
AlwNI CAG_nnn'CTG
Cuts at: 1353 1765 3203 1353
Size: 412 1438 2610
Fragments arranged by size:
2610 1438 412
ApaLI G'TGCA_C
Cuts at: 3298 3298
Size: 4460
ApoI r'AATT_y
Cuts at: 1108 1191 1251 1464 3813 4069 1108
Size: 83 60 213 2349 256 1499
Fragments arranged by size:
2349 1499 256 213 83 60
AseI AT'TA_AT
Cuts at: 20 20
Size: 4460
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
AvaI C'yCGr_G
Cuts at: 1480 1480
Size: 4460
BamHI G'GATC_C
Cuts at: 1485 1485
Size: 4460
BanI G'GyrC_C
Cuts at: 808 1476 1890 2138 2173 808
Size: 668 414 248 35 3095
Fragments arranged by size:
3095 668 414 248 35
BanII G_rGCy'C
Cuts at: 1184 1474 2504 1184
Size: 290 1030 3140
Fragments arranged by size:
3140 1030 290
BbeI G_GCGC'C
Cuts at: 1894 2142 1894
Size: 248 4212
BciVI GTATCCnnnnn_n'
Cuts at: 2358 3414 4460 2358
Size: 1056 1046 2358
Fragments arranged by size:
2358 1056 1046
BclI T'GATC_A
Cuts at: 924 1980 924
Size: 1056 3404
BglII A'GATC_T
Cuts at: 653 1975 4012 653
Size: 1322 2037 1101
Fragments arranged by size:
2037 1322 1101
BlpI GC'TnA_GC
Cuts at: 115 174 1572 115
Size: 59 1398 3003
Fragments arranged by size:
3003 1398 59
Bme1580I G_kGCm'C
Cuts at: 641 974 2085 2178 3302 641
Size: 333 1111 93 1124 1799
Fragments arranged by size:
1799 1124 1111 333 93
BmrI ACTGGGnnnn_n'
Cuts at: 1672 1753 2088 4232 1672
Size: 81 335 2144 1900
Fragments arranged by size:
2144 1900 335 81
BmtI G_CTAG'C
Cuts at: 1346 1346
Size: 4460
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1413 1413
Size: 4460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1636 2515 3229 3527 1636
Size: 879 714 298 2569
Fragments arranged by size:
2569 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 887 4164 887
Size: 3277 1183
BsaAI yAC'GTr
Cuts at: 1813 1813
Size: 4460
BsaBI GATnn'nnATC
Cuts at: 6 1974 4011 6
Size: 1968 2037 455
Fragments arranged by size:
2037 1968 455
BsaHI Gr'CG_yC
Cuts at: 1891 2139 2841 4337 1891
Size: 248 702 1496 2014
Fragments arranged by size:
2014 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1090 1649 2170 2848 3259 3406 3680 1090
Size: 559 521 678 411 147 274 1870
Fragments arranged by size:
1870 678 559 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 794 794
Size: 4460
BseYI C'CCAG_C
Cuts at: 784 1885 2643 3308 3716 784
Size: 1101 758 665 408 1528
Fragments arranged by size:
1528 1101 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 598 598
Size: 4460
BsiEI CG_ry'CG
Cuts at: 104 2002 2048 3278 104
Size: 1898 46 1230 1286
Fragments arranged by size:
1898 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 121 964 1184 1474 2252 2442 3302 121
Size: 843 220 290 778 190 860 1279
Fragments arranged by size:
1279 860 843 778 290 220 190
Bsp1286I G_dGCh'C
Cuts at: 121 641 964 974 1184 1474 2085 2178 2252
Size: 520 323 10 210 290 611 93 74
Cuts at: 2252 2442 2504 3302 121
Size: 190 62 798 1279
Fragments arranged by size:
1279 798 611 520 323 290 210 190
93 74 62 10
BspHI T'CATG_A
Cuts at: 1119 2892 1119
Size: 1773 2687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1510 1724 2026 2407 1510
Size: 214 302 381 3563
Fragments arranged by size:
3563 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2752 2806 4290 4455 50
Size: 2702 54 1484 165 55
Fragments arranged by size:
2702 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2372 2372
Size: 4460
BsrFI r'CCGG_y
Cuts at: 1090 2458 2639 1090
Size: 1368 181 2911
Fragments arranged by size:
2911 1368 181
BsrGI T'GTAC_A
Cuts at: 978 978
Size: 4460
BssSI C'ACGA_G
Cuts at: 2731 3439 2731
Size: 708 3752
BstAPI GCAn_nnn'nTGC
Cuts at: 1837 1837
Size: 4460
BstBI TT'CG_AA
Cuts at: 681 2821 681
Size: 2140 2320
BstEII G'GTnAC_C
Cuts at: 992 992
Size: 4460
BstYI r'GATC_y
Cuts at: 1 212 653 1485 1952 1975 2310 2556 2862
Size: 211 441 832 467 23 335 246 306
Cuts at: 2862 2874 2960 4012 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 832 467 449 441 335 306 246
211 86 23 12
BtgI C'CryG_G
Cuts at: 338 338
Size: 4460
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2410 2591 2754 2410
Size: 181 163 4116
Fragments arranged by size:
4116 181 163
BtsI GCAGTG_nn'
Cuts at: 532 1703 532
Size: 1171 3289
DraI TTT'AAA
Cuts at: 1459 1459
Size: 4460
DrdI GACnn_nn'nnGTC
Cuts at: 2166 3510 2166
Size: 1344 3116
EaeI y'GGCC_r
Cuts at: 724 2045 2219 2610 2637 3982 724
Size: 1321 174 391 27 1345 1202
Fragments arranged by size:
1345 1321 1202 391 174 27
EagI C'GGCC_G
Cuts at: 2045 2045
Size: 4460
EarI CTCTTCn'nnn_
Cuts at: 293 476 885 1189 2483 2693 293
Size: 183 409 304 1294 210 2060
Fragments arranged by size:
2060 1294 409 304 210 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2668 3410 3556 4270 2668
Size: 742 146 714 2858
Fragments arranged by size:
2858 742 714 146
EcoICRI GAG'CTC
Cuts at: 1182 1472 1182
Size: 290 4170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 695 1413 2285 2717 3070 3732 317
Size: 378 718 872 432 353 662 1045
Fragments arranged by size:
1045 872 718 662 432 378 353
EcoNI CCTnn'n_nnAGG
Cuts at: 738 738
Size: 4460
EcoO109I rG'GnC_Cy
Cuts at: 156 730 1336 1599 156
Size: 574 606 263 3017
Fragments arranged by size:
3017 606 574 263
EcoRI G'AATT_C
Cuts at: 1251 1464 1251
Size: 213 4247
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4460
FspI TGC'GCA
Cuts at: 2241 2241
Size: 4460
HaeII r_GCGC'y
Cuts at: 1280 1894 2142 3372 4143 1280
Size: 614 248 1230 771 1597
Fragments arranged by size:
1597 1230 771 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 931 963 1426 1458 2302 2332 2334 2364 931
Size: 32 463 32 844 30 2 30 3027
Fragments arranged by size:
3027 844 463 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1499 1499
Size: 4460
KasI G'GCGC_C
Cuts at: 1890 2138 1890
Size: 248 4212
KpnI G_GTAC'C
Cuts at: 1480 1480
Size: 4460
MluI A'CGCG_T
Cuts at: 3893 3893
Size: 4460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 488 1340 3219 3403 488
Size: 852 1879 184 1545
Fragments arranged by size:
1879 1545 852 184
MscI TGG'CCA
Cuts at: 2221 2221
Size: 4460
MslI CAynn'nnrTG
Cuts at: 715 1124 1172 1232 2449 2576 715
Size: 409 48 60 1217 127 2599
Fragments arranged by size:
2599 1217 409 127 60 48
MspA1I CmG'CkG
Cuts at: 723 1353 1571 1885 2245 3029 3274 723
Size: 630 218 314 360 784 245 1909
Fragments arranged by size:
1909 784 630 360 314 245 218
NaeI GCC'GGC
Cuts at: 2641 2641
Size: 4460
NarI GG'CG_CC
Cuts at: 1891 2139 1891
Size: 248 4212
NcoI C'CATG_G
Cuts at: 338 338
Size: 4460
NgoMIV G'CCGG_C
Cuts at: 2639 2639
Size: 4460
NheI G'CTAG_C
Cuts at: 1342 1342
Size: 4460
NspI r_CATG'y
Cuts at: 1513 2013 3616 1513
Size: 500 1603 2357
Fragments arranged by size:
2357 1603 500
PciI A'CATG_T
Cuts at: 3612 3612
Size: 4460
PflMI CCAn_nnn'nTGG
Cuts at: 1296 1296
Size: 4460
PmeI GTTT'AAAC
Cuts at: 1459 1459
Size: 4460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 662 694 2906 2938 662
Size: 32 2212 32 2184
Fragments arranged by size:
2212 2184 32 32
PpuMI rG'GwC_Cy
Cuts at: 730 1336 730
Size: 606 3854
PsiI TTA'TAA
Cuts at: 1025 1025
Size: 4460
PstI C_TGCA'G
Cuts at: 1203 1421 1507 2192 1203
Size: 218 86 685 3471
Fragments arranged by size:
3471 685 218 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4460
PvuII CAG'CTG
Cuts at: 1353 1885 2245 1353
Size: 532 360 3568
Fragments arranged by size:
3568 532 360
RsrII CG'GwC_CG
Cuts at: 2655 2655
Size: 4460
SacI G_AGCT'C
Cuts at: 1184 1474 1184
Size: 290 4170
SalI G'TCGA_C
Cuts at: 1497 1497
Size: 4460
SapI GCTCTTCn'nnn_
Cuts at: 1189 2483 2693 1189
Size: 1294 210 2956
Fragments arranged by size:
2956 1294 210
SbfI CC_TGCA'GG
Cuts at: 1507 1507
Size: 4460
ScaI AGT'ACT
Cuts at: 3977 3977
Size: 4460
SfcI C'TryA_G
Cuts at: 33 471 1199 1256 1417 1503 2188 3156 3347
Size: 438 728 57 161 86 685 968 191
Cuts at: 3347 33
Size: 1146
Fragments arranged by size:
1146 968 728 685 438 191 161 86
57
SfoI GGC'GCC
Cuts at: 1892 2140 1892
Size: 248 4212
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4460
SmaI CCC'GGG
Cuts at: 1482 1482
Size: 4460
SmlI C'TyrA_G
Cuts at: 1615 2530 3244 3506 1615
Size: 915 714 262 2569
Fragments arranged by size:
2569 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4460
SphI G_CATG'C
Cuts at: 1513 2013 1513
Size: 500 3960
StuI AGG'CCT
Cuts at: 610 610
Size: 4460
StyI C'CwwG_G
Cuts at: 338 1594 1948 338
Size: 1256 354 2850
Fragments arranged by size:
2850 1256 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 381 2043 2176 2635 2844 381
Size: 1662 133 459 209 1997
Fragments arranged by size:
1997 1662 459 209 133
TatI w'GTAC_w
Cuts at: 978 1056 3975 978
Size: 78 2919 1463
Fragments arranged by size:
2919 1463 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 789 821 3977 4009 789
Size: 32 3156 32 1240
Fragments arranged by size:
3156 1240 32 32
Tth111I GACn'n_nGTC
Cuts at: 2257 2257
Size: 4460
XbaI T'CTAG_A
Cuts at: 1491 1491
Size: 4460
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1327 1354 1955 1327
Size: 27 601 3832
Fragments arranged by size:
3832 601 27
XmaI C'CCGG_G
Cuts at: 1480 1480
Size: 4460
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PflMI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI