Restriction map for
pF1KB4899
(Circular) (Six-base) MAPSORT of: pF1KB4899.seq Check: 675 from: 1 to: 4520
pF1KB4899 4520 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 1408 1408
Size: 4520
AccI GT'mk_AC
Cuts at: 1558 1558
Size: 4520
Acc65I G'GTAC_C
Cuts at: 1536 1536
Size: 4520
AclI AA'CG_TT
Cuts at: 4360 4360
Size: 4520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 2345 2777 3130 3792 351
Size: 1994 432 353 662 1079
Fragments arranged by size:
1994 1079 662 432 353
AfeI AGC'GCT
Cuts at: 1417 1417
Size: 4520
AflIII A'CryG_T
Cuts at: 1421 1872 3672 3953 1421
Size: 451 1800 281 1988
Fragments arranged by size:
1988 1800 451 281
AlwNI CAG_nnn'CTG
Cuts at: 522 1825 3263 522
Size: 1303 1438 1779
Fragments arranged by size:
1779 1438 1303
ApaLI G'TGCA_C
Cuts at: 3358 3358
Size: 4520
ApoI r'AATT_y
Cuts at: 648 897 1524 3873 4129 648
Size: 249 627 2349 256 1039
Fragments arranged by size:
2349 1039 627 256 249
AseI AT'TA_AT
Cuts at: 20 20
Size: 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
AvaI C'yCGr_G
Cuts at: 1468 1540 1468
Size: 72 4448
BamHI G'GATC_C
Cuts at: 1545 1545
Size: 4520
BanI G'GyrC_C
Cuts at: 392 558 714 1536 1950 2198 2233 392
Size: 166 156 822 414 248 35 2679
Fragments arranged by size:
2679 822 414 248 166 156 35
BanII G_rGCy'C
Cuts at: 973 1534 2564 973
Size: 561 1030 2929
Fragments arranged by size:
2929 1030 561
BbeI G_GCGC'C
Cuts at: 396 718 1954 2202 396
Size: 322 1236 248 2714
Fragments arranged by size:
2714 1236 322 248
BciVI GTATCCnnnnn_n'
Cuts at: 2418 3474 4520 2418
Size: 1056 1046 2418
Fragments arranged by size:
2418 1056 1046
BclI T'GATC_A
Cuts at: 605 2040 605
Size: 1435 3085
BglI GCCn_nnn'nGGC
Cuts at: 151 151
Size: 4520
BglII A'GATC_T
Cuts at: 2035 4072 2035
Size: 2037 2483
BlpI GC'TnA_GC
Cuts at: 838 1357 1632 838
Size: 519 275 3726
Fragments arranged by size:
3726 519 275
Bme1580I G_kGCm'C
Cuts at: 2145 2238 3362 2145
Size: 93 1124 3303
Fragments arranged by size:
3303 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1732 1813 2148 4292 1732
Size: 81 335 2144 1960
Fragments arranged by size:
2144 1960 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 190 2887 190
Size: 2697 1823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 616 970 1237 1283 1429 571
Size: 45 354 267 46 146 3662
Fragments arranged by size:
3662 354 267 146 46 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1696 2575 3289 3587 160
Size: 1536 879 714 298 1093
Fragments arranged by size:
1536 1093 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4224 4224
Size: 4520
BsaAI yAC'GTr
Cuts at: 1422 1873 1422
Size: 451 4069
BsaBI GATnn'nnATC
Cuts at: 6 2034 4071 6
Size: 2028 2037 455
Fragments arranged by size:
2037 2028 455
BsaHI Gr'CG_yC
Cuts at: 393 715 922 1405 1493 1951 2199 2901 4397
Size: 322 207 483 88 458 248 702 1496
Cuts at: 4397 393
Size: 516
Fragments arranged by size:
1496 702 516 483 458 322 248 207
88
BsaWI w'CCGG_w
Cuts at: 1709 2230 2908 3319 3466 3740 1709
Size: 521 678 411 147 274 2489
Fragments arranged by size:
2489 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 922 952 646
Size: 30 246 30 4214
Fragments arranged by size:
4214 246 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 973 1134 973
Size: 161 4359
BseYI C'CCAG_C
Cuts at: 787 1455 1945 2703 3368 3776 787
Size: 668 490 758 665 408 1531
Fragments arranged by size:
1531 758 668 665 490 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 1462 348
Size: 1114 3406
BsiEI CG_ry'CG
Cuts at: 104 2062 2108 3338 104
Size: 1958 46 1230 1286
Fragments arranged by size:
1958 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 973 1534 2312 2502 3362 973
Size: 561 778 190 860 2131
Fragments arranged by size:
2131 860 778 561 190
BsmI GAATG_Cn'
Cuts at: 401 731 401
Size: 330 4190
BsmBI CGTCTCn'nnnn_
Cuts at: 1259 1259
Size: 4520
Bsp1286I G_dGCh'C
Cuts at: 973 1534 2145 2238 2312 2502 2564 3362 973
Size: 561 611 93 74 190 62 798 2131
Fragments arranged by size:
2131 798 611 561 190 93 74 62
BspHI T'CATG_A
Cuts at: 288 2952 288
Size: 2664 1856
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1164 1570 1784 2086 2467 1164
Size: 406 214 302 381 3217
Fragments arranged by size:
3217 406 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2812 2866 4350 4515 50
Size: 2762 54 1484 165 55
Fragments arranged by size:
2762 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2432 2432
Size: 4520
BsrFI r'CCGG_y
Cuts at: 1171 1310 2518 2699 1171
Size: 139 1208 181 2992
Fragments arranged by size:
2992 1208 181 139
BssSI C'ACGA_G
Cuts at: 2791 3499 2791
Size: 708 3812
BstAPI GCAn_nnn'nTGC
Cuts at: 252 261 1897 252
Size: 9 1636 2875
Fragments arranged by size:
2875 1636 9
BstBI TT'CG_AA
Cuts at: 1049 2881 1049
Size: 1832 2688
BstYI r'GATC_y
Cuts at: 1 1545 2012 2035 2370 2616 2922 2934 3020
Size: 1544 467 23 335 246 306 12 86
Cuts at: 3020 4072 1
Size: 1052 449
Fragments arranged by size:
1544 1052 467 449 335 306 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2470 2651 2814 2470
Size: 181 163 4176
Fragments arranged by size:
4176 181 163
BtsI GCAGTG_nn'
Cuts at: 250 1763 250
Size: 1513 3007
DraI TTT'AAA
Cuts at: 1519 1519
Size: 4520
DrdI GACnn_nn'nnGTC
Cuts at: 2226 3570 2226
Size: 1344 3176
EaeI y'GGCC_r
Cuts at: 513 1169 2105 2279 2670 2697 4042 513
Size: 656 936 174 391 27 1345 991
Fragments arranged by size:
1345 991 936 656 391 174 27
EagI C'GGCC_G
Cuts at: 2105 2105
Size: 4520
EarI CTCTTCn'nnn_
Cuts at: 236 2543 2753 236
Size: 2307 210 2003
Fragments arranged by size:
2307 2003 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 285 2728 3470 3616 4330 285
Size: 2443 742 146 714 475
Fragments arranged by size:
2443 742 714 475 146
EcoICRI GAG'CTC
Cuts at: 971 1532 971
Size: 561 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 351 571 616 970 1237 1283 1429 2345 2777
Size: 220 45 354 267 46 146 916 432
Cuts at: 2777 3130 3792 351
Size: 353 662 1079
Fragments arranged by size:
1079 916 662 432 354 353 267 220
146 46 45
EcoNI CCTnn'n_nnAGG
Cuts at: 535 535
Size: 4520
EcoO109I rG'GnC_Cy
Cuts at: 209 1659 209
Size: 1450 3070
EcoRI G'AATT_C
Cuts at: 1524 1524
Size: 4520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 561 2539 561
Size: 1978 2542
FspI TGC'GCA
Cuts at: 2301 2301
Size: 4520
HaeII r_GCGC'y
Cuts at: 183 396 718 1419 1954 2202 3432 4203 183
Size: 213 322 701 535 248 1230 771 500
Fragments arranged by size:
1230 771 701 535 500 322 248 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 618 650 922 954 1210 1242 2362 2392 2394
Size: 32 272 32 256 32 1120 30 2
Cuts at: 2394 2424 618
Size: 30 2714
Fragments arranged by size:
2714 1120 272 256 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 320 1559 320
Size: 1239 3281
HpaI GTT'AAC
Cuts at: 320 320
Size: 4520
KasI G'GCGC_C
Cuts at: 392 714 1950 2198 392
Size: 322 1236 248 2714
Fragments arranged by size:
2714 1236 322 248
KpnI G_GTAC'C
Cuts at: 1540 1540
Size: 4520
MluI A'CGCG_T
Cuts at: 3953 3953
Size: 4520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3279 3463 3279
Size: 184 4336
MscI TGG'CCA
Cuts at: 515 2281 515
Size: 1766 2754
MslI CAynn'nnrTG
Cuts at: 2509 2636 2509
Size: 127 4393
MspA1I CmG'CkG
Cuts at: 390 1162 1200 1256 1631 1945 2305 3089 3334
Size: 772 38 56 375 314 360 784 245
Cuts at: 3334 390
Size: 1576
Fragments arranged by size:
1576 784 772 375 360 314 245 56
38
NaeI GCC'GGC
Cuts at: 1173 2701 1173
Size: 1528 2992
NarI GG'CG_CC
Cuts at: 393 715 1951 2199 393
Size: 322 1236 248 2714
Fragments arranged by size:
2714 1236 322 248
NgoMIV G'CCGG_C
Cuts at: 1171 2699 1171
Size: 1528 2992
NspI r_CATG'y
Cuts at: 771 1573 2073 3676 771
Size: 802 500 1603 1615
Fragments arranged by size:
1615 1603 802 500
PciI A'CATG_T
Cuts at: 3672 3672
Size: 4520
PfoI T'CCnGG_A
Cuts at: 758 991 1214 758
Size: 233 223 4064
Fragments arranged by size:
4064 233 223
PmeI GTTT'AAAC
Cuts at: 1519 1519
Size: 4520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 2966
Size: 32 4488
PpuMI rG'GwC_Cy
Cuts at: 209 209
Size: 4520
PstI C_TGCA'G
Cuts at: 497 1063 1161 1567 2252 497
Size: 566 98 406 685 2765
Fragments arranged by size:
2765 685 566 406 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4520
PvuII CAG'CTG
Cuts at: 1162 1200 1256 1945 2305 1162
Size: 38 56 689 360 3377
Fragments arranged by size:
3377 689 360 56 38
RsrII CG'GwC_CG
Cuts at: 2715 2715
Size: 4520
SacI G_AGCT'C
Cuts at: 973 1534 973
Size: 561 3959
SalI G'TCGA_C
Cuts at: 1557 1557
Size: 4520
SanDI GG'GwC_CC
Cuts at: 209 209
Size: 4520
SapI GCTCTTCn'nnn_
Cuts at: 236 2543 2753 236
Size: 2307 210 2003
Fragments arranged by size:
2307 2003 210
SbfI CC_TGCA'GG
Cuts at: 1567 1567
Size: 4520
ScaI AGT'ACT
Cuts at: 4037 4037
Size: 4520
SexAI A'CCwGG_T
Cuts at: 137 137
Size: 4520
SfcI C'TryA_G
Cuts at: 33 493 1059 1157 1563 2248 3216 3407 33
Size: 460 566 98 406 685 968 191 1146
Fragments arranged by size:
1146 968 685 566 460 406 191 98
SfoI GGC'GCC
Cuts at: 394 716 1952 2200 394
Size: 322 1236 248 2714
Fragments arranged by size:
2714 1236 322 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4520
SgrAI Cr'CCGG_yG
Cuts at: 1310 1310
Size: 4520
SmaI CCC'GGG
Cuts at: 1542 1542
Size: 4520
SmlI C'TyrA_G
Cuts at: 175 1675 2590 3304 3566 175
Size: 1500 915 714 262 1129
Fragments arranged by size:
1500 1129 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4520
SphI G_CATG'C
Cuts at: 1573 2073 1573
Size: 500 4020
StyI C'CwwG_G
Cuts at: 531 1654 2008 531
Size: 1123 354 3043
Fragments arranged by size:
3043 1123 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 801 2103 2236 2695 2904 562
Size: 239 1302 133 459 209 2178
Fragments arranged by size:
2178 1302 459 239 209 133
TatI w'GTAC_w
Cuts at: 4035 4035
Size: 4520
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 651 678 683 754 786 4037 4069 646
Size: 5 27 5 71 32 3251 32 1097
Fragments arranged by size:
3251 1097 71 32 32 27 5 5
Tth111I GACn'n_nGTC
Cuts at: 2317 2317
Size: 4520
XbaI T'CTAG_A
Cuts at: 1551 1551
Size: 4520
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 413 2015 413
Size: 1602 2918
XmaI C'CCGG_G
Cuts at: 1540 1540
Size: 4520
XmnI GAAnn'nnTTC
Cuts at: 115 545 115
Size: 430 4090
ZraI GAC'GTC
Cuts at: 1406 1406
Size: 4520
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BspEI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SfiI SnaBI SrfI SspI StuI
SwaI XhoI