Restriction map for
pF1KB4907
(Circular) (Six-base) MAPSORT of: pF1KB4907.seq Check: 5829 from: 1 to: 4319
pF1KB4907 4319 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 382 382
Size: 4319
AccI GT'mk_AC
Cuts at: 695 1357 695
Size: 662 3657
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 1129 2144 2576 2929 3591 494
Size: 635 1015 432 353 662 1222
Fragments arranged by size:
1222 1015 662 635 432 353
AflIII A'CryG_T
Cuts at: 1671 3471 3752 1671
Size: 1800 281 2238
Fragments arranged by size:
2238 1800 281
AleI CACnn'nnGTG
Cuts at: 1061 1061
Size: 4319
AlwNI CAG_nnn'CTG
Cuts at: 424 1624 3062 424
Size: 1200 1438 1681
Fragments arranged by size:
1681 1438 1200
ApaI G_GGCC'C
Cuts at: 548 548
Size: 4319
ApaLI G'TGCA_C
Cuts at: 3157 3157
Size: 4319
ApoI r'AATT_y
Cuts at: 627 1323 3672 3928 627
Size: 696 2349 256 1018
Fragments arranged by size:
2349 1018 696 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 791 1339 791
Size: 548 3771
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 488 521 907 940 1137 1170 488
Size: 33 386 33 197 33 3637
Fragments arranged by size:
3637 386 197 33 33 33
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 684 1335 1749 1997 2032 684
Size: 651 414 248 35 2971
Fragments arranged by size:
2971 651 414 248 35
BanII G_rGCy'C
Cuts at: 302 548 1333 2363 302
Size: 246 785 1030 2258
Fragments arranged by size:
2258 1030 785 246
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BbsI GAAGACnn'nnnn_
Cuts at: 455 455
Size: 4319
BciVI GTATCCnnnnn_n'
Cuts at: 1063 2217 3273 4319 1063
Size: 1154 1056 1046 1063
Fragments arranged by size:
1154 1063 1056 1046
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 1431 1431
Size: 4319
Bme1580I G_kGCm'C
Cuts at: 548 1944 2037 3161 548
Size: 1396 93 1124 1706
Fragments arranged by size:
1706 1396 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 400 922 1531 1612 1947 4091 400
Size: 522 609 81 335 2144 628
Fragments arranged by size:
2144 628 609 522 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 1023 524
Size: 499 3820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1495 2374 3088 3386 1495
Size: 879 714 298 2428
Fragments arranged by size:
2428 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 236 4023 236
Size: 3787 532
BsaAI yAC'GTr
Cuts at: 1672 1672
Size: 4319
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 379 1011 1750 1998 2700 4196 379
Size: 632 739 248 702 1496 502
Fragments arranged by size:
1496 739 702 632 502 248
BsaWI w'CCGG_w
Cuts at: 1118 1508 2029 2707 3118 3265 3539 1118
Size: 390 521 678 411 147 274 1898
Fragments arranged by size:
1898 678 521 411 390 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 298 328 298
Size: 30 4289
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 420 420
Size: 4319
BseYI C'CCAG_C
Cuts at: 1744 2502 3167 3575 1744
Size: 758 665 408 2488
Fragments arranged by size:
2488 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1152 1273 1152
Size: 121 4198
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3137 104
Size: 1757 46 1230 1286
Fragments arranged by size:
1757 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1006 1333 2111 2301 3161 1006
Size: 327 778 190 860 2164
Fragments arranged by size:
2164 860 778 327 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1011 1011
Size: 4319
Bsp1286I G_dGCh'C
Cuts at: 302 548 1006 1333 1944 2037 2111 2301 2363
Size: 246 458 327 611 93 74 190 62
Cuts at: 2363 3161 302
Size: 798 1460
Fragments arranged by size:
1460 798 611 458 327 246 190 93
74 62
BspHI T'CATG_A
Cuts at: 2751 2751
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 848 1369 1583 1885 2266 848
Size: 521 214 302 381 2901
Fragments arranged by size:
2901 521 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2611 2665 4149 4314 50
Size: 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 2231
Size: 4319
BsrFI r'CCGG_y
Cuts at: 186 2317 2498 186
Size: 2131 181 2007
Fragments arranged by size:
2131 2007 181
BsrGI T'GTAC_A
Cuts at: 1088 1088
Size: 4319
BssSI C'ACGA_G
Cuts at: 2590 3298 2590
Size: 708 3611
BstAPI GCAn_nnn'nTGC
Cuts at: 588 1696 588
Size: 1108 3211
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstEII G'GTnAC_C
Cuts at: 710 710
Size: 4319
BstYI r'GATC_y
Cuts at: 1 877 1344 1811 1834 2169 2415 2721 2733
Size: 876 467 467 23 335 246 306 12
Cuts at: 2733 2819 3871 1
Size: 86 1052 449
Fragments arranged by size:
1052 876 467 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 668 768 668
Size: 100 4219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 1562 1562
Size: 4319
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 3369 2025
Size: 1344 2975
EaeI y'GGCC_r
Cuts at: 1904 2078 2469 2496 3841 1904
Size: 174 391 27 1345 2382
Fragments arranged by size:
2382 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 118 459 2342 2552 118
Size: 341 1883 210 1885
Fragments arranged by size:
1885 1883 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 3269 3415 4129 2527
Size: 742 146 714 2717
Fragments arranged by size:
2717 742 714 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 524 1023 1129 2144 2576 2929 3591 494
Size: 30 499 106 1015 432 353 662 1222
Fragments arranged by size:
1222 1015 662 499 432 353 106 30
EcoO109I rG'GnC_Cy
Cuts at: 544 545 1458 544
Size: 1 913 3405
Fragments arranged by size:
3405 913 1
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 4319
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 1753 2001 3231 4002 1753
Size: 248 1230 771 2070
Fragments arranged by size:
2070 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 2161 2191 2193 2223 301
Size: 32 1828 30 2 30 2397
Fragments arranged by size:
2397 1828 32 30 30 2
HincII GTy'rAC
Cuts at: 527 1358 527
Size: 831 3488
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 552 3078 3262 552
Size: 2526 184 1609
Fragments arranged by size:
2526 1609 184
MscI TGG'CCA
Cuts at: 2080 2080
Size: 4319
MslI CAynn'nnrTG
Cuts at: 667 1061 1239 2308 2435 667
Size: 394 178 1069 127 2551
Fragments arranged by size:
2551 1069 394 178 127
MspA1I CmG'CkG
Cuts at: 703 1430 1744 2104 2888 3133 703
Size: 727 314 360 784 245 1889
Fragments arranged by size:
1889 784 727 360 314 245
NaeI GCC'GGC
Cuts at: 188 2500 188
Size: 2312 2007
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NcoI C'CATG_G
Cuts at: 768 768
Size: 4319
NdeI CA'TA_TG
Cuts at: 119 1304 119
Size: 1185 3134
NgoMIV G'CCGG_C
Cuts at: 186 2498 186
Size: 2312 2007
NspI r_CATG'y
Cuts at: 1372 1872 3475 1372
Size: 500 1603 2216
Fragments arranged by size:
2216 1603 500
PciI A'CATG_T
Cuts at: 3471 3471
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 4287
PspOMI G'GGCC_C
Cuts at: 544 544
Size: 4319
PstI C_TGCA'G
Cuts at: 1366 2051 1366
Size: 685 3634
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 703 1744 2104 703
Size: 1041 360 2918
Fragments arranged by size:
2918 1041 360
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 320 1362 2047 3015 3206 33
Size: 287 1042 685 968 191 1146
Fragments arranged by size:
1146 1042 968 685 287 191
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 1474 2389 3103 3365 1474
Size: 915 714 262 2428
Fragments arranged by size:
2428 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StuI AGG'CCT
Cuts at: 797 797
Size: 4319
StyI C'CwwG_G
Cuts at: 768 1453 1807 768
Size: 685 354 3280
Fragments arranged by size:
3280 685 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1902 2035 2494 2703 1902
Size: 133 459 209 3518
Fragments arranged by size:
3518 459 209 133
TatI w'GTAC_w
Cuts at: 906 1088 3834 906
Size: 182 2746 1391
Fragments arranged by size:
2746 1391 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4287
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1814 1814
Size: 4319
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
XmnI GAAnn'nnTTC
Cuts at: 266 1234 266
Size: 968 3351
ZraI GAC'GTC
Cuts at: 380 380
Size: 4319
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BspEI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI