Restriction map for
pF1KB4919
(Circular) (Six-base) MAPSORT of: pF1KB4919.seq Check: 8334 from: 1 to: 8612
pF1KB4919 8612 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 861 1607 1671 861
Size: 746 64 7802
Fragments arranged by size:
7802 746 64
AccI GT'mk_AC
Cuts at: 5388 5650 5388
Size: 262 8350
Acc65I G'GTAC_C
Cuts at: 5628 5628
Size: 8612
AclI AA'CG_TT
Cuts at: 8452 8452
Size: 8612
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 587 752 1509 1642 2948 3160 4127 4340
Size: 430 165 757 133 1306 212 967 213
Cuts at: 4340 4803 4884 6437 6869 7616 7884 157
Size: 463 81 1553 432 747 268 885
Fragments arranged by size:
1553 1306 967 885 757 747 463 432
430 268 213 212 165 133 81
AflIII A'CryG_T
Cuts at: 5364 5604 5964 7068 8045 5364
Size: 240 360 1104 977 5931
Fragments arranged by size:
5931 1104 977 360 240
AgeI A'CCGG_T
Cuts at: 7000 7000
Size: 8612
AhdI GACnn_n'nnGTC
Cuts at: 624 624
Size: 8612
AleI CACnn'nnGTG
Cuts at: 427 427
Size: 8612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1619 2605 2911 1619
Size: 986 306 7320
Fragments arranged by size:
7320 986 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2253 2284 2285 2252
Size: 1 31 1 8579
Fragments arranged by size:
8579 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2451 3877 5917 7484 2451
Size: 1426 2040 1567 3579
Fragments arranged by size:
3579 2040 1567 1426
ApaLI G'TGCA_C
Cuts at: 5360 7382 5360
Size: 2022 6590
ApoI r'AATT_y
Cuts at: 1018 3377 3891 5616 7965 8221 1018
Size: 2359 514 1725 2349 256 1409
Fragments arranged by size:
2359 2349 1725 1409 514 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8612
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8612
AvaI C'yCGr_G
Cuts at: 1710 2909 5305 5632 1710
Size: 1199 2396 327 4690
Fragments arranged by size:
4690 2396 1199 327
AvrII C'CTAG_G
Cuts at: 633 4501 633
Size: 3868 4744
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2276 2309 3708 3741 2276
Size: 33 1399 33 7147
Fragments arranged by size:
7147 1399 33 33
BamHI G'GATC_C
Cuts at: 294 2279 3565 4967 5637 294
Size: 1985 1286 1402 670 3269
Fragments arranged by size:
3269 1985 1402 1286 670
BanI G'GyrC_C
Cuts at: 303 1625 2913 4120 4953 5628 6042 6290 6325
Size: 1322 1288 1207 833 675 414 248 35
Cuts at: 6325 303
Size: 2590
Fragments arranged by size:
2590 1322 1288 1207 833 675 414 248
35
BanII G_rGCy'C
Cuts at: 822 1711 2618 3013 4941 5339 5626 6656 822
Size: 889 907 395 1928 398 287 1030 2778
Fragments arranged by size:
2778 1928 1030 907 889 398 395 287
BbeI G_GCGC'C
Cuts at: 4124 6046 6294 4124
Size: 1922 248 6442
Fragments arranged by size:
6442 1922 248
BbsI GAAGACnn'nnnn_
Cuts at: 2855 4890 5210 2855
Size: 2035 320 6257
Fragments arranged by size:
6257 2035 320
BbvCI CC'TCA_GC
Cuts at: 3968 4082 5415 3968
Size: 114 1333 7165
Fragments arranged by size:
7165 1333 114
BciVI GTATCCnnnnn_n'
Cuts at: 1907 6510 7271 8612 1907
Size: 4603 761 1341 1907
Fragments arranged by size:
4603 1907 1341 761
BclI T'GATC_A
Cuts at: 3948 4172 6132 3948
Size: 224 1960 6428
Fragments arranged by size:
6428 1960 224
BfrBI ATG'CAT
Cuts at: 3399 3399
Size: 8612
BglII A'GATC_T
Cuts at: 4509 6127 8164 4509
Size: 1618 2037 4957
Fragments arranged by size:
4957 2037 1618
BlpI GC'TnA_GC
Cuts at: 1705 1928 1973 5724 1705
Size: 223 45 3751 4593
Fragments arranged by size:
4593 3751 223 45
Bme1580I G_kGCm'C
Cuts at: 2916 4958 5364 6237 6330 7386 2916
Size: 2042 406 873 93 1056 4142
Fragments arranged by size:
4142 2042 1056 873 406 93
BmgBI CAC'GTC
Cuts at: 3649 3649
Size: 8612
BmrI ACTGGGnnnn_n'
Cuts at: 796 3179 4220 5824 5905 6240 8384 796
Size: 2383 1041 1604 81 335 2144 1024
Fragments arranged by size:
2383 2144 1604 1041 1024 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1134 4383 5097 6979 1134
Size: 3249 714 1882 2767
Fragments arranged by size:
3249 2767 1882 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1182 1612 2582 4394 4848 1182
Size: 430 970 1812 454 4946
Fragments arranged by size:
4946 1812 970 454 430
Bpu10I CC'TnA_GC
Cuts at: 1253 3968 4082 5393 5415 1253
Size: 2715 114 1311 22 4450
Fragments arranged by size:
4450 2715 1311 114 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 2822 4377 5788 6667 7159 7457 279
Size: 2543 1555 1411 879 492 298 1434
Fragments arranged by size:
2543 1555 1434 1411 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1036 4643 4826 8316 1036
Size: 3607 183 3490 1332
Fragments arranged by size:
3607 3490 1332 183
BsaAI yAC'GTr
Cuts at: 2292 5965 2292
Size: 3673 4939
BsaBI GATnn'nnATC
Cuts at: 6 6126 8163 6
Size: 6120 2037 455
Fragments arranged by size:
6120 2037 455
BsaHI Gr'CG_yC
Cuts at: 4121 6043 6291 6993 8489 4121
Size: 1922 248 702 1496 4244
Fragments arranged by size:
4244 1922 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1613 2902 5276 5801 6322 7000 7274 7421 7832
Size: 1289 2374 525 521 678 274 147 411
Cuts at: 7832 1613
Size: 2393
Fragments arranged by size:
2393 2374 1289 678 525 521 411 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 607 967 1107 1643 2695 2805 2820 4089 4354
Size: 360 140 536 1052 110 15 1269 265
Cuts at: 4354 4359 5065 5157 5162 607
Size: 5 706 92 5 4057
Fragments arranged by size:
4057 1269 1052 706 536 360 265 140
110 92 15 5 5
BseYI C'CCAG_C
Cuts at: 1052 1168 1386 1730 2236 2451 2747 2884 3112
Size: 116 218 344 506 215 296 137 228
Cuts at: 3112 6037 6795 7372 7868 1052
Size: 2925 758 577 496 1796
Fragments arranged by size:
2925 1796 758 577 506 496 344 296
228 218 215 137 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 895 1229 1705 5350 857
Size: 38 334 476 3645 4119
Fragments arranged by size:
4119 3645 476 334 38
BsiEI CG_ry'CG
Cuts at: 104 6154 6200 7408 104
Size: 6050 46 1208 1308
Fragments arranged by size:
6050 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1115 1598 1711 2057 2618 5197 5364 5626 6404
Size: 483 113 346 561 2579 167 262 778
Cuts at: 6404 6594 7386 1115
Size: 190 792 2341
Fragments arranged by size:
2579 2341 792 778 561 483 346 262
190 167 113
BsmI GAATG_Cn'
Cuts at: 1275 1881 2594 1275
Size: 606 713 7293
Fragments arranged by size:
7293 713 606
BsmBI CGTCTCn'nnnn_
Cuts at: 2980 2980
Size: 8612
Bsp1286I G_dGCh'C
Cuts at: 822 1115 1598 1711 2057 2618 2916 3013 4941
Size: 293 483 113 346 561 298 97 1928
Cuts at: 4941 4958 5197 5339 5364 5626 6237 6330 6404
Size: 17 239 142 25 262 611 93 74
Cuts at: 6404 6594 6656 7386 822
Size: 190 62 730 2048
Fragments arranged by size:
2048 1928 730 611 561 483 346 298
293 262 239 190 142 113 97 93
74 62 25 17
BspEI T'CCGG_A
Cuts at: 5276 7832 5276
Size: 2556 6056
BspHI T'CATG_A
Cuts at: 7788 7788
Size: 8612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 861 1607 1671 2079 2193 5662 5876 6178 6559
Size: 746 64 408 114 3469 214 302 381
Cuts at: 6559 861
Size: 2914
Fragments arranged by size:
3469 2914 746 408 381 302 214 114
64
BsrBI CCG'CTC
Cuts at: 50 5226 6904 6958 8442 8607 50
Size: 5176 1678 54 1484 165 55
Fragments arranged by size:
5176 1678 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 307 2438 5023 5264 6524 307
Size: 2131 2585 241 1260 2395
Fragments arranged by size:
2585 2395 2131 1260 241
BsrFI r'CCGG_y
Cuts at: 3412 6610 6791 7000 3412
Size: 3198 181 209 5024
Fragments arranged by size:
5024 3198 209 181
BsrGI T'GTAC_A
Cuts at: 2315 5254 2315
Size: 2939 5673
BssSI C'ACGA_G
Cuts at: 6883 7241 6883
Size: 358 8254
BstAPI GCAn_nnn'nTGC
Cuts at: 4932 5989 4932
Size: 1057 7555
BstBI TT'CG_AA
Cuts at: 6973 6973
Size: 8612
BstEII G'GTnAC_C
Cuts at: 2403 2403
Size: 8612
BstXI CCAn_nnnn'nTGG
Cuts at: 2610 2610
Size: 8612
BstYI r'GATC_y
Cuts at: 1 294 2279 3565 4509 4967 5577 5637 6104
Size: 293 1985 1286 944 458 610 60 467
Cuts at: 6104 6127 6462 6708 7720 7806 7818 8164 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1985 1286 1012 944 610 467 458 449
346 335 293 246 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 1119 4805 4877 1119
Size: 3686 72 4854
Fragments arranged by size:
4854 3686 72
BtgI C'CryG_G
Cuts at: 299 497 1061 1469 1629 4131 4739 4949 5285
Size: 198 564 408 160 2502 608 210 336
Cuts at: 5285 299
Size: 3626
Fragments arranged by size:
3626 2502 608 564 408 336 210 198
160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6562 6743 6906 6562
Size: 181 163 8268
Fragments arranged by size:
8268 181 163
BtsI GCAGTG_nn'
Cuts at: 1717 3368 5855 1717
Size: 1651 2487 4474
Fragments arranged by size:
4474 2487 1651
ClaI AT'CG_AT
Cuts at: 2981 2981
Size: 8612
DraI TTT'AAA
Cuts at: 269 5611 269
Size: 5342 3270
DraIII CAC_nnn'GTG
Cuts at: 4228 4228
Size: 8612
DrdI GACnn_nn'nnGTC
Cuts at: 184 6318 7176 184
Size: 6134 858 1620
Fragments arranged by size:
6134 1620 858
EaeI y'GGCC_r
Cuts at: 2564 3651 6197 6371 6762 6789 8134 2564
Size: 1087 2546 174 391 27 1345 3042
Fragments arranged by size:
3042 2546 1345 1087 391 174 27
EagI C'GGCC_G
Cuts at: 6197 6197
Size: 8612
EarI CTCTTCn'nnn_
Cuts at: 364 380 1661 1981 2623 3047 3143 3350 4554
Size: 16 1281 320 642 424 96 207 1204
Cuts at: 4554 4641 4659 4788 5285 6635 6845 364
Size: 87 18 129 497 1350 210 2131
Fragments arranged by size:
2131 1350 1281 1204 642 497 424 320
210 207 129 96 87 18 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6820 7130 7276 8422 6820
Size: 310 146 1146 7010
Fragments arranged by size:
7010 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1709 2616 5624 1709
Size: 907 3008 4697
Fragments arranged by size:
4697 3008 907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 587 752 1182 1509 1612 1642 2582 2948
Size: 430 165 430 327 103 30 940 366
Cuts at: 2948 3160 4127 4340 4394 4803 4848 4884 6437
Size: 212 967 213 54 409 45 36 1553
Cuts at: 6437 6869 7616 7884 157
Size: 432 747 268 885
Fragments arranged by size:
1553 967 940 885 747 432 430 430
409 366 327 268 213 212 165 103
54 45 36 30
EcoNI CCTnn'n_nnAGG
Cuts at: 4374 5396 4374
Size: 1022 7590
EcoO109I rG'GnC_Cy
Cuts at: 1668 1690 2724 4777 4882 5088 5125 5751 1668
Size: 22 1034 2053 105 206 37 626 4529
Fragments arranged by size:
4529 2053 1034 626 206 105 37 22
EcoRI G'AATT_C
Cuts at: 5616 5616
Size: 8612
EcoRV GAT'ATC
Cuts at: 2979 4635 2979
Size: 1656 6956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1518 6631 1518
Size: 5113 3499
FspI TGC'GCA
Cuts at: 6393 6393
Size: 8612
HaeII r_GCGC'y
Cuts at: 4124 4699 6046 6294 7316 8295 4124
Size: 575 1347 248 1022 979 4441
Fragments arranged by size:
4441 1347 1022 979 575 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 610 642 1362 1394 2209 2241 2977 3009 3550
Size: 32 720 32 815 32 736 32 541
Cuts at: 3550 3582 4928 4960 5569 5601 6454 6484 6486
Size: 32 1346 32 609 32 853 30 2
Cuts at: 6486 6516 610
Size: 30 2706
Fragments arranged by size:
2706 1346 853 815 736 720 609 541
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2183 5651 2183
Size: 3468 5144
HindIII A'AGCT_T
Cuts at: 1237 1517 3418 1237
Size: 280 1901 6431
Fragments arranged by size:
6431 1901 280
KasI G'GCGC_C
Cuts at: 4120 6042 6290 4120
Size: 1922 248 6442
Fragments arranged by size:
6442 1922 248
KpnI G_GTAC'C
Cuts at: 5632 5632
Size: 8612
MfeI C'AATT_G
Cuts at: 4766 4766
Size: 8612
MluI A'CGCG_T
Cuts at: 8045 8045
Size: 8612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 172 1809 5507 7283 7467 172
Size: 1637 3698 1776 184 1317
Fragments arranged by size:
3698 1776 1637 1317 184
MscI TGG'CCA
Cuts at: 2566 6373 2566
Size: 3807 4805
MslI CAynn'nnrTG
Cuts at: 397 427 1601 1747 3004 3151 3734 5441 6601
Size: 30 1174 146 1257 147 583 1707 1160
Cuts at: 6601 6728 397
Size: 127 2281
Fragments arranged by size:
2281 1707 1257 1174 1160 583 147 146
127 30
MspA1I CmG'CkG
Cuts at: 1063 1390 1590 1704 2751 3048 3116 5723 6037
Size: 327 200 114 1047 297 68 2607 314
Cuts at: 6037 6397 7410 7655 1063
Size: 360 1013 245 2020
Fragments arranged by size:
2607 2020 1047 1013 360 327 314 297
245 200 114 68
NaeI GCC'GGC
Cuts at: 6793 6793
Size: 8612
NarI GG'CG_CC
Cuts at: 4121 6043 6291 4121
Size: 1922 248 6442
Fragments arranged by size:
6442 1922 248
NcoI C'CATG_G
Cuts at: 497 1629 4739 4949 497
Size: 1132 3110 210 4160
Fragments arranged by size:
4160 3110 1132 210
NdeI CA'TA_TG
Cuts at: 2365 3803 2365
Size: 1438 7174
NgoMIV G'CCGG_C
Cuts at: 6791 6791
Size: 8612
NsiI A_TGCA'T
Cuts at: 3401 3401
Size: 8612
NspI r_CATG'y
Cuts at: 330 1606 2047 2464 3090 3708 3933 4350 5368
Size: 1276 441 417 626 618 225 417 1018
Cuts at: 5368 5608 5665 6165 7072 330
Size: 240 57 500 907 1870
Fragments arranged by size:
1870 1276 1018 907 626 618 500 441
417 417 240 225 57
PasI CC'CwG_GG
Cuts at: 1548 1672 2571 4155 4376 5352 1548
Size: 124 899 1584 221 976 4808
Fragments arranged by size:
4808 1584 976 899 221 124
PciI A'CATG_T
Cuts at: 5364 5604 7068 5364
Size: 240 1464 6908
Fragments arranged by size:
6908 1464 240
PflMI CCAn_nnn'nTGG
Cuts at: 2302 2451 2785 2302
Size: 149 334 8129
Fragments arranged by size:
8129 334 149
PfoI T'CCnGG_A
Cuts at: 651 4410 651
Size: 3759 4853
PmeI GTTT'AAAC
Cuts at: 5611 5611
Size: 8612
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7751 7783 7751
Size: 32 8580
PpuMI rG'GwC_Cy
Cuts at: 1668 1690 2724 4882 5088 5125 1668
Size: 22 1034 2158 206 37 5155
Fragments arranged by size:
5155 2158 1034 206 37 22
PshAI GACnn'nnGTC
Cuts at: 548 2401 548
Size: 1853 6759
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 111 143 111
Size: 32 8580
PstI C_TGCA'G
Cuts at: 409 467 566 3375 5659 6344 409
Size: 58 99 2809 2284 685 2677
Fragments arranged by size:
2809 2677 2284 685 99 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8612
PvuII CAG'CTG
Cuts at: 1590 2751 3048 6037 6397 1590
Size: 1161 297 2989 360 3805
Fragments arranged by size:
3805 2989 1161 360 297
RsrII CG'GwC_CG
Cuts at: 1616 6807 1616
Size: 5191 3421
SacI G_AGCT'C
Cuts at: 1711 2618 5626 1711
Size: 907 3008 4697
Fragments arranged by size:
4697 3008 907
SacII CC_GC'GG
Cuts at: 1064 1064
Size: 8612
SalI G'TCGA_C
Cuts at: 5649 5649
Size: 8612
SapI GCTCTTCn'nnn_
Cuts at: 364 1981 2623 3350 6635 6845 364
Size: 1617 642 727 3285 210 2131
Fragments arranged by size:
3285 2131 1617 727 642 210
SbfI CC_TGCA'GG
Cuts at: 5659 5659
Size: 8612
ScaI AGT'ACT
Cuts at: 8129 8129
Size: 8612
SfcI C'TryA_G
Cuts at: 33 240 405 463 562 1901 3371 5655 6340
Size: 207 165 58 99 1339 1470 2284 685
Cuts at: 6340 7333 7524 33
Size: 993 191 1121
Fragments arranged by size:
2284 1470 1339 1121 993 685 207 191
165 99 58
SfoI GGC'GCC
Cuts at: 4122 6044 6292 4122
Size: 1922 248 6442
Fragments arranged by size:
6442 1922 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8612
SmaI CCC'GGG
Cuts at: 2911 5634 2911
Size: 2723 5889
SmlI C'TyrA_G
Cuts at: 258 2837 4392 5767 6682 7174 7436 258
Size: 2579 1555 1375 915 492 262 1434
Fragments arranged by size:
2579 1555 1434 1375 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3504 67
Size: 3437 5175
SphI G_CATG'C
Cuts at: 1606 2464 3090 3708 4350 5665 6165 1606
Size: 858 626 618 642 1315 500 4053
Fragments arranged by size:
4053 1315 858 642 626 618 500
SspI AAT'ATT
Cuts at: 286 3599 286
Size: 3313 5299
StuI AGG'CCT
Cuts at: 1154 3405 1154
Size: 2251 6361
StyI C'CwwG_G
Cuts at: 497 633 1629 4501 4606 4739 4949 5084 5746
Size: 136 996 2872 105 133 210 135 662
Cuts at: 5746 6100 497
Size: 354 3009
Fragments arranged by size:
3009 2872 996 662 354 210 136 135
133 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 964 1092 1400 2150 4239 4533 5034 5437 6195
Size: 128 308 750 2089 294 501 403 758
Cuts at: 6195 6328 6787 6996 964
Size: 133 459 209 2580
Fragments arranged by size:
2580 2089 758 750 501 459 403 308
294 209 133 128
TatI w'GTAC_w
Cuts at: 2315 3662 5254 8127 2315
Size: 1347 1592 2873 2800
Fragments arranged by size:
2873 2800 1592 1347
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2281 2313 2660 2692 4327 4359 8129 8161 2281
Size: 32 347 32 1635 32 3770 32 2732
Fragments arranged by size:
3770 2732 1635 347 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4894 6409 4894
Size: 1515 7097
XbaI T'CTAG_A
Cuts at: 5643 5643
Size: 8612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 565 1555 3120 3336 6107 565
Size: 990 1565 216 2771 3070
Fragments arranged by size:
3070 2771 1565 990 216
XmaI C'CCGG_G
Cuts at: 2909 5632 2909
Size: 2723 5889
XmnI GAAnn'nnTTC
Cuts at: 1019 2592 1019
Size: 1573 7039
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII ApaI AscI BcgI BglI BmtI
BsaXI BsiWI BssHII BstZ17I CspCI FseI FspAI HpaI
NheI NotI NruI PacI PmlI PsiI PspOMI PspXI
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI