Restriction map for
pF1KB4940
(Circular) (Six-base) MAPSORT of: pF1KB4940.seq Check: 2597 from: 1 to: 4550
pF1KB4940 4550 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 208 556 1364 208
Size: 348 808 3394
Fragments arranged by size:
3394 808 348
AccI GT'mk_AC
Cuts at: 1588 1588
Size: 4550
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 2375 2807 3160 3822 820
Size: 1555 432 353 662 1548
Fragments arranged by size:
1555 1548 662 432 353
AflIII A'CryG_T
Cuts at: 1902 3702 3983 1902
Size: 1800 281 2469
Fragments arranged by size:
2469 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 209 1064 209
Size: 855 3695
AleI CACnn'nnGTG
Cuts at: 1298 1370 1298
Size: 72 4478
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 811 811
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 1094 1855 3293 1094
Size: 761 1438 2351
Fragments arranged by size:
2351 1438 761
ApaI G_GGCC'C
Cuts at: 1145 1145
Size: 4550
ApaLI G'TGCA_C
Cuts at: 960 3388 960
Size: 2428 2122
ApoI r'AATT_y
Cuts at: 1414 1554 3903 4159 1414
Size: 140 2349 256 1805
Fragments arranged by size:
2349 1805 256 140
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 585 693 1137 1570 585
Size: 108 444 433 3565
Fragments arranged by size:
3565 444 433 108
BamHI G'GATC_C
Cuts at: 1575 1575
Size: 4550
BanI G'GyrC_C
Cuts at: 220 372 594 897 1463 1566 1980 2228 2263
Size: 152 222 303 566 103 414 248 35
Cuts at: 2263 220
Size: 2507
Fragments arranged by size:
2507 566 414 303 248 222 152 103
35
BanII G_rGCy'C
Cuts at: 251 691 716 1145 1211 1244 1564 2594 251
Size: 440 25 429 66 33 320 1030 2207
Fragments arranged by size:
2207 1030 440 429 320 66 33 25
BbeI G_GCGC'C
Cuts at: 376 598 901 1984 2232 376
Size: 222 303 1083 248 2694
Fragments arranged by size:
2694 1083 303 248 222
BbsI GAAGACnn'nnnn_
Cuts at: 208 1266 208
Size: 1058 3492
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3504 4550 2448
Size: 1056 1046 2448
Fragments arranged by size:
2448 1056 1046
BclI T'GATC_A
Cuts at: 2070 2070
Size: 4550
BglI GCCn_nnn'nGGC
Cuts at: 349 695 875 349
Size: 346 180 4024
Fragments arranged by size:
4024 346 180
BglII A'GATC_T
Cuts at: 2065 4102 2065
Size: 2037 2513
BlpI GC'TnA_GC
Cuts at: 811 1081 1662 811
Size: 270 581 3699
Fragments arranged by size:
3699 581 270
Bme1580I G_kGCm'C
Cuts at: 225 931 964 1145 1466 2175 2268 3392 225
Size: 706 33 181 321 709 93 1124 1383
Fragments arranged by size:
1383 1124 709 706 321 181 93 33
BmgBI CAC'GTC
Cuts at: 684 684
Size: 4550
BmrI ACTGGGnnnn_n'
Cuts at: 268 1404 1762 1843 2178 4322 268
Size: 1136 358 81 335 2144 496
Fragments arranged by size:
2144 1136 496 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 267 637 969 2917 267
Size: 370 332 1948 1900
Fragments arranged by size:
1948 1900 370 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 349
Size: 4550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1090 1330 1726 2605 3319 3617 1090
Size: 240 396 879 714 298 2023
Fragments arranged by size:
2023 879 714 396 298 240
BsaI GGTCTCn'nnnn_
Cuts at: 385 957 4254 385
Size: 572 3297 681
Fragments arranged by size:
3297 681 572
BsaAI yAC'GTr
Cuts at: 926 1903 926
Size: 977 3573
BsaBI GATnn'nnATC
Cuts at: 6 2064 4101 6
Size: 2058 2037 455
Fragments arranged by size:
2058 2037 455
BsaHI Gr'CG_yC
Cuts at: 373 595 805 898 1981 2229 2931 4427 373
Size: 222 210 93 1083 248 702 1496 496
Fragments arranged by size:
1496 1083 702 496 248 222 210 93
BsaWI w'CCGG_w
Cuts at: 456 1739 2260 2938 3349 3496 3770 456
Size: 1283 521 678 411 147 274 1236
Fragments arranged by size:
1283 1236 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 438 468 438
Size: 30 4520
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 412 489 610 1252 259
Size: 153 77 121 642 3557
Fragments arranged by size:
3557 642 153 121 77
BseYI C'CCAG_C
Cuts at: 1042 1975 2733 3398 3806 1042
Size: 933 758 665 408 1786
Fragments arranged by size:
1786 933 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 995
Size: 4550
BsiEI CG_ry'CG
Cuts at: 104 288 314 1006 1267 1494 2092 2138 3368
Size: 184 26 692 261 227 598 46 1230
Cuts at: 3368 104
Size: 1286
Fragments arranged by size:
1286 1230 692 598 261 227 184 46
26
BsiHKAI G_wGCw'C
Cuts at: 251 716 964 1211 1564 2342 2532 3392 251
Size: 465 248 247 353 778 190 860 1409
Fragments arranged by size:
1409 860 778 465 353 248 247 190
Bsp1286I G_dGCh'C
Cuts at: 225 251 691 716 931 964 1145 1211 1244
Size: 26 440 25 215 33 181 66 33
Cuts at: 1244 1466 1564 2175 2268 2342 2532 2594 3392
Size: 222 98 611 93 74 190 62 798
Cuts at: 3392 225
Size: 1383
Fragments arranged by size:
1383 798 611 440 222 215 190 181
98 93 74 66 62 33 33 26
25
BspEI T'CCGG_A
Cuts at: 456 456
Size: 4550
BspHI T'CATG_A
Cuts at: 2982 2982
Size: 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 208 556 1364 1600 1814 2116 2497 208
Size: 348 808 236 214 302 381 2261
Fragments arranged by size:
2261 808 381 348 302 236 214
BsrBI CCG'CTC
Cuts at: 50 335 640 1194 1424 2842 2896 4380 4545
Size: 285 305 554 230 1418 54 1484 165
Cuts at: 4545 50
Size: 55
Fragments arranged by size:
1484 1418 554 305 285 230 165 55
54
BsrDI GCAATG_nn'
Cuts at: 999 2462 999
Size: 1463 3087
BsrFI r'CCGG_y
Cuts at: 343 1001 1005 2548 2729 343
Size: 658 4 1543 181 2164
Fragments arranged by size:
2164 1543 658 181 4
BsrGI T'GTAC_A
Cuts at: 740 740
Size: 4550
BssHII G'CGCG_C
Cuts at: 536 536
Size: 4550
BssSI C'ACGA_G
Cuts at: 2821 3529 2821
Size: 708 3842
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstXI CCAn_nnnn'nTGG
Cuts at: 327 327
Size: 4550
BstYI r'GATC_y
Cuts at: 1 1575 2042 2065 2400 2646 2952 2964 3050
Size: 1574 467 23 335 246 306 12 86
Cuts at: 3050 4102 1
Size: 1052 449
Fragments arranged by size:
1574 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 314 314
Size: 4550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 205 1793 205
Size: 1588 2962
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DraIII CAC_nnn'GTG
Cuts at: 888 888
Size: 4550
DrdI GACnn_nn'nnGTC
Cuts at: 139 2256 3600 139
Size: 2117 1344 1089
Fragments arranged by size:
2117 1344 1089
EaeI y'GGCC_r
Cuts at: 285 317 467 707 721 996 1003 2135 2309
Size: 32 150 240 14 275 7 1132 174
Cuts at: 2309 2700 2727 4072 285
Size: 391 27 1345 763
Fragments arranged by size:
1345 1132 763 391 275 240 174 150
32 27 14 7
EagI C'GGCC_G
Cuts at: 285 1003 2135 285
Size: 718 1132 2700
Fragments arranged by size:
2700 1132 718
EarI CTCTTCn'nnn_
Cuts at: 507 960 1528 2573 2783 507
Size: 453 568 1045 210 2274
Fragments arranged by size:
2274 1045 568 453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 353 626 685 737 2758 3500 3646 4360 353
Size: 273 59 52 2021 742 146 714 543
Fragments arranged by size:
2021 742 714 543 273 146 59 52
EcoICRI GAG'CTC
Cuts at: 249 714 1209 1562 249
Size: 465 495 353 3237
Fragments arranged by size:
3237 495 465 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 820 2375 2807 3160 3822 349
Size: 471 1555 432 353 662 1077
Fragments arranged by size:
1555 1077 662 471 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1141 1689 1141
Size: 548 4002
EcoRI G'AATT_C
Cuts at: 1554 1554
Size: 4550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 376 598 818 901 977 1984 2232 3462 4233
Size: 222 220 83 76 1007 248 1230 771
Cuts at: 4233 376
Size: 693
Fragments arranged by size:
1230 1007 771 693 248 222 220 83
76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 438 470 971 1003 1050 1082 1326
Size: 32 211 32 501 32 47 32 244
Cuts at: 1326 1358 2392 2422 2424 2454 195
Size: 32 1034 30 2 30 2291
Fragments arranged by size:
2291 1034 501 244 211 47 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1589 1589
Size: 4550
KasI G'GCGC_C
Cuts at: 372 594 897 1980 2228 372
Size: 222 303 1083 248 2694
Fragments arranged by size:
2694 1083 303 248 222
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 673 1490 3309 3493 673
Size: 817 1819 184 1730
Fragments arranged by size:
1819 1730 817 184
MscI TGG'CCA
Cuts at: 319 998 2311 319
Size: 679 1313 2558
Fragments arranged by size:
2558 1313 679
MslI CAynn'nnrTG
Cuts at: 1298 1370 2539 2666 1298
Size: 72 1169 127 3182
Fragments arranged by size:
3182 1169 127 72
MspA1I CmG'CkG
Cuts at: 236 524 1661 1975 2335 3119 3364 236
Size: 288 1137 314 360 784 245 1422
Fragments arranged by size:
1422 1137 784 360 314 288 245
NaeI GCC'GGC
Cuts at: 1007 2731 1007
Size: 1724 2826
NarI GG'CG_CC
Cuts at: 373 595 898 1981 2229 373
Size: 222 303 1083 248 2694
Fragments arranged by size:
2694 1083 303 248 222
NgoMIV G'CCGG_C
Cuts at: 1005 2729 1005
Size: 1724 2826
NspI r_CATG'y
Cuts at: 1233 1603 2103 3706 1233
Size: 370 500 1603 2077
Fragments arranged by size:
2077 1603 500 370
PasI CC'CwG_GG
Cuts at: 1026 1062 1026
Size: 36 4514
PciI A'CATG_T
Cuts at: 3702 3702
Size: 4550
PflMI CCAn_nnn'nTGG
Cuts at: 888 888
Size: 4550
PfoI T'CCnGG_A
Cuts at: 413 413
Size: 4550
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PmlI CAC'GTG
Cuts at: 926 926
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 4518
PspOMI G'GGCC_C
Cuts at: 1141 1141
Size: 4550
PstI C_TGCA'G
Cuts at: 553 1597 2282 553
Size: 1044 685 2821
Fragments arranged by size:
2821 1044 685
PvuI CG_AT'CG
Cuts at: 104 1267 104
Size: 1163 3387
PvuII CAG'CTG
Cuts at: 1975 2335 1975
Size: 360 4190
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 251 716 1211 1564 251
Size: 465 495 353 3237
Fragments arranged by size:
3237 495 465 353
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 2573 2783 2573
Size: 210 4340
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SfcI C'TryA_G
Cuts at: 33 549 1593 2278 3246 3437 33
Size: 516 1044 685 968 191 1146
Fragments arranged by size:
1146 1044 968 685 516 191
SfoI GGC'GCC
Cuts at: 374 596 899 1982 2230 374
Size: 222 303 1083 248 2694
Fragments arranged by size:
2694 1083 303 248 222
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 587 1139 1572 587
Size: 552 433 3565
Fragments arranged by size:
3565 552 433
SmlI C'TyrA_G
Cuts at: 1069 1345 1705 2620 3334 3596 1069
Size: 276 360 915 714 262 2023
Fragments arranged by size:
2023 915 714 360 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1233 1603 2103 1233
Size: 370 500 3680
Fragments arranged by size:
3680 500 370
StuI AGG'CCT
Cuts at: 617 617
Size: 4550
StyI C'CwwG_G
Cuts at: 1684 2038 1684
Size: 354 4196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 834 979 1306 1508 2133 2266 2725 2934
Size: 425 145 327 202 625 133 459 209
Cuts at: 2934 409
Size: 2025
Fragments arranged by size:
2025 625 459 425 327 209 202 145
133
TatI w'GTAC_w
Cuts at: 740 4065 740
Size: 3325 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1004 1036 4067 4099 1004
Size: 32 3031 32 1455
Fragments arranged by size:
3031 1455 32 32
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 973 2045 973
Size: 1072 3478
XmaI C'CCGG_G
Cuts at: 585 1137 1570 585
Size: 552 433 3565
Fragments arranged by size:
3565 552 433
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI