Restriction map for
pF1KB4944
(Circular) (Six-base) MAPSORT of: pF1KB4944.seq Check: 2608 from: 1 to: 4649
pF1KB4944 4649 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1687 1687
Size: 4649
Acc65I G'GTAC_C
Cuts at: 1665 1665
Size: 4649
AclI AA'CG_TT
Cuts at: 4489 4489
Size: 4649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2474 2906 3259 3921 1462
Size: 1012 432 353 662 2190
Fragments arranged by size:
2190 1012 662 432 353
AflIII A'CryG_T
Cuts at: 2001 3801 4082 2001
Size: 1800 281 2568
Fragments arranged by size:
2568 1800 281
AleI CACnn'nnGTG
Cuts at: 1565 1565
Size: 4649
AlwNI CAG_nnn'CTG
Cuts at: 1954 3392 1954
Size: 1438 3211
ApaLI G'TGCA_C
Cuts at: 3487 3487
Size: 4649
ApoI r'AATT_y
Cuts at: 895 1653 4002 4258 895
Size: 758 2349 256 1286
Fragments arranged by size:
2349 1286 758 256
AseI AT'TA_AT
Cuts at: 20 509 1526 20
Size: 489 1017 3143
Fragments arranged by size:
3143 1017 489
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
AvaI C'yCGr_G
Cuts at: 1451 1669 1451
Size: 218 4431
BamHI G'GATC_C
Cuts at: 1674 1674
Size: 4649
BanI G'GyrC_C
Cuts at: 1665 2079 2327 2362 1665
Size: 414 248 35 3952
Fragments arranged by size:
3952 414 248 35
BanII G_rGCy'C
Cuts at: 936 1663 2693 936
Size: 727 1030 2892
Fragments arranged by size:
2892 1030 727
BbeI G_GCGC'C
Cuts at: 2083 2331 2083
Size: 248 4401
BbsI GAAGACnn'nnnn_
Cuts at: 328 640 829 328
Size: 312 189 4148
Fragments arranged by size:
4148 312 189
BbvCI CC'TCA_GC
Cuts at: 1590 1590
Size: 4649
BciVI GTATCCnnnnn_n'
Cuts at: 2547 3603 4649 2547
Size: 1056 1046 2547
Fragments arranged by size:
2547 1056 1046
BclI T'GATC_A
Cuts at: 2169 2169
Size: 4649
BglII A'GATC_T
Cuts at: 2164 4201 2164
Size: 2037 2612
BlpI GC'TnA_GC
Cuts at: 1761 1761
Size: 4649
Bme1580I G_kGCm'C
Cuts at: 2274 2367 3491 2274
Size: 93 1124 3432
Fragments arranged by size:
3432 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1861 1942 2277 4421 1861
Size: 81 335 2144 2089
Fragments arranged by size:
2144 2089 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4649
Bpu10I CC'TnA_GC
Cuts at: 1590 1590
Size: 4649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1342 1825 2704 3418 3716 1342
Size: 483 879 714 298 2275
Fragments arranged by size:
2275 879 714 483 298
BsaI GGTCTCn'nnnn_
Cuts at: 4353 4353
Size: 4649
BsaAI yAC'GTr
Cuts at: 2002 2002
Size: 4649
BsaBI GATnn'nnATC
Cuts at: 6 2163 4200 6
Size: 2157 2037 455
Fragments arranged by size:
2157 2037 455
BsaHI Gr'CG_yC
Cuts at: 1394 2080 2328 3030 4526 1394
Size: 686 248 702 1496 1517
Fragments arranged by size:
1517 1496 702 686 248
BsaWI w'CCGG_w
Cuts at: 1490 1838 2359 3037 3448 3595 3869 1490
Size: 348 521 678 411 147 274 2270
Fragments arranged by size:
2270 678 521 411 348 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 741 771 741
Size: 30 4619
BseYI C'CCAG_C
Cuts at: 952 2074 2832 3497 3905 952
Size: 1122 758 665 408 1696
Fragments arranged by size:
1696 1122 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 144 2191 2237 3467 104
Size: 40 2047 46 1230 1286
Fragments arranged by size:
2047 1286 1230 46 40
BsiHKAI G_wGCw'C
Cuts at: 836 865 1202 1663 2441 2631 3491 836
Size: 29 337 461 778 190 860 1994
Fragments arranged by size:
1994 860 778 461 337 190 29
BsmI GAATG_Cn'
Cuts at: 556 1541 556
Size: 985 3664
Bsp1286I G_dGCh'C
Cuts at: 836 865 936 1202 1663 2274 2367 2441 2631
Size: 29 71 266 461 611 93 74 190
Cuts at: 2631 2693 3491 836
Size: 62 798 1994
Fragments arranged by size:
1994 798 611 461 266 190 93 74
71 62 29
BspEI T'CCGG_A
Cuts at: 1490 1490
Size: 4649
BspHI T'CATG_A
Cuts at: 546 1079 3081 546
Size: 533 2002 2114
Fragments arranged by size:
2114 2002 533
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 1699 1913 2215 2596 791
Size: 908 214 302 381 2844
Fragments arranged by size:
2844 908 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2941 2995 4479 4644 50
Size: 2891 54 1484 165 55
Fragments arranged by size:
2891 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 822 2561 822
Size: 1739 2910
BsrFI r'CCGG_y
Cuts at: 2647 2828 2647
Size: 181 4468
BsrGI T'GTAC_A
Cuts at: 687 965 687
Size: 278 4371
BssSI C'ACGA_G
Cuts at: 2920 3628 2920
Size: 708 3941
BstAPI GCAn_nnn'nTGC
Cuts at: 2026 2026
Size: 4649
BstBI TT'CG_AA
Cuts at: 3010 3010
Size: 4649
BstXI CCAn_nnnn'nTGG
Cuts at: 952 952
Size: 4649
BstYI r'GATC_y
Cuts at: 1 1487 1674 2141 2164 2499 2745 3051 3063
Size: 1486 187 467 23 335 246 306 12
Cuts at: 3063 3149 4201 1
Size: 86 1052 449
Fragments arranged by size:
1486 1052 467 449 335 306 246 187
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 310 310
Size: 4649
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2599 2780 2943 2599
Size: 181 163 4305
Fragments arranged by size:
4305 181 163
BtsI GCAGTG_nn'
Cuts at: 1892 1892
Size: 4649
DraI TTT'AAA
Cuts at: 233 503 1648 233
Size: 270 1145 3234
Fragments arranged by size:
3234 1145 270
DrdI GACnn_nn'nnGTC
Cuts at: 2355 3699 2355
Size: 1344 3305
EaeI y'GGCC_r
Cuts at: 2234 2408 2799 2826 4171 2234
Size: 174 391 27 1345 2712
Fragments arranged by size:
2712 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2234 2234
Size: 4649
EarI CTCTTCn'nnn_
Cuts at: 260 967 2672 2882 260
Size: 707 1705 210 2027
Fragments arranged by size:
2027 1705 707 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2857 3599 3745 4459 2857
Size: 742 146 714 3047
Fragments arranged by size:
3047 742 714 146
EcoICRI GAG'CTC
Cuts at: 1661 1661
Size: 4649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2474 2906 3259 3921 1462
Size: 1012 432 353 662 2190
Fragments arranged by size:
2190 1012 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1362 1788 1362
Size: 426 4223
EcoRI G'AATT_C
Cuts at: 1653 1653
Size: 4649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4649
FspI TGC'GCA
Cuts at: 1610 2430 1610
Size: 820 3829
HaeII r_GCGC'y
Cuts at: 123 2083 2331 3561 4332 123
Size: 1960 248 1230 771 440
Fragments arranged by size:
1960 1230 771 440 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 498 530 2491 2521 2523 2553 498
Size: 32 1961 30 2 30 2594
Fragments arranged by size:
2594 1961 32 30 30 2
HincII GTy'rAC
Cuts at: 1688 1688
Size: 4649
KasI G'GCGC_C
Cuts at: 2079 2327 2079
Size: 248 4401
KpnI G_GTAC'C
Cuts at: 1669 1669
Size: 4649
MluI A'CGCG_T
Cuts at: 4082 4082
Size: 4649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 196 387 3408 3592 196
Size: 191 3021 184 1253
Fragments arranged by size:
3021 1253 191 184
MscI TGG'CCA
Cuts at: 2410 2410
Size: 4649
MslI CAynn'nnrTG
Cuts at: 1183 1565 1601 2638 2765 1183
Size: 382 36 1037 127 3067
Fragments arranged by size:
3067 1037 382 127 36
MspA1I CmG'CkG
Cuts at: 1760 2074 2434 3218 3463 1760
Size: 314 360 784 245 2946
Fragments arranged by size:
2946 784 360 314 245
NaeI GCC'GGC
Cuts at: 2830 2830
Size: 4649
NarI GG'CG_CC
Cuts at: 2080 2328 2080
Size: 248 4401
NdeI CA'TA_TG
Cuts at: 1207 1207
Size: 4649
NgoMIV G'CCGG_C
Cuts at: 2828 2828
Size: 4649
NspI r_CATG'y
Cuts at: 1606 1702 2202 3805 1606
Size: 96 500 1603 2450
Fragments arranged by size:
2450 1603 500 96
PciI A'CATG_T
Cuts at: 3801 3801
Size: 4649
PfoI T'CCnGG_A
Cuts at: 112 112
Size: 4649
PmeI GTTT'AAAC
Cuts at: 1648 1648
Size: 4649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 4617
PstI C_TGCA'G
Cuts at: 888 1696 2381 888
Size: 808 685 3156
Fragments arranged by size:
3156 808 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4649
PvuII CAG'CTG
Cuts at: 2074 2434 2074
Size: 360 4289
RsrII CG'GwC_CG
Cuts at: 2844 2844
Size: 4649
SacI G_AGCT'C
Cuts at: 1663 1663
Size: 4649
SalI G'TCGA_C
Cuts at: 1686 1686
Size: 4649
SapI GCTCTTCn'nnn_
Cuts at: 967 2672 2882 967
Size: 1705 210 2734
Fragments arranged by size:
2734 1705 210
SbfI CC_TGCA'GG
Cuts at: 1696 1696
Size: 4649
ScaI AGT'ACT
Cuts at: 4166 4166
Size: 4649
SfcI C'TryA_G
Cuts at: 33 873 884 1256 1692 2377 3345 3536 33
Size: 840 11 372 436 685 968 191 1146
Fragments arranged by size:
1146 968 840 685 436 372 191 11
SfoI GGC'GCC
Cuts at: 2081 2329 2081
Size: 248 4401
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
SmaI CCC'GGG
Cuts at: 1453 1671 1453
Size: 218 4431
SmlI C'TyrA_G
Cuts at: 1357 1804 2719 3433 3695 1357
Size: 447 915 714 262 2311
Fragments arranged by size:
2311 915 714 447 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4649
SphI G_CATG'C
Cuts at: 1702 2202 1702
Size: 500 4149
SspI AAT'ATT
Cuts at: 604 604
Size: 4649
StyI C'CwwG_G
Cuts at: 827 1623 1783 2137 827
Size: 796 160 354 3339
Fragments arranged by size:
3339 796 354 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 433 1535 2232 2365 2824 3033 433
Size: 1102 697 133 459 209 2049
Fragments arranged by size:
2049 1102 697 459 209 133
TatI w'GTAC_w
Cuts at: 687 965 4164 687
Size: 278 3199 1172
Fragments arranged by size:
3199 1172 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 975 1007 4166 4198 975
Size: 32 3159 32 1426
Fragments arranged by size:
3159 1426 32 32
Tth111I GACn'n_nGTC
Cuts at: 145 2446 145
Size: 2301 2348
XbaI T'CTAG_A
Cuts at: 1680 1680
Size: 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1115 1302 2144 1115
Size: 187 842 3620
Fragments arranged by size:
3620 842 187
XmaI C'CCGG_G
Cuts at: 1451 1669 1451
Size: 218 4431
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI