Restriction map for
pF1KB4957
(Circular) (Six-base) MAPSORT of: pF1KB4957.seq Check: 9512 from: 1 to: 4715
pF1KB4957 4715 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1753 1753
Size: 4715
Acc65I G'GTAC_C
Cuts at: 1731 1731
Size: 4715
AclI AA'CG_TT
Cuts at: 4555 4555
Size: 4715
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1535 2540 2972 3719 3987 878
Size: 657 1005 432 747 268 1606
Fragments arranged by size:
1606 1005 747 657 432 268
AflII C'TTAA_G
Cuts at: 415 415
Size: 4715
AflIII A'CryG_T
Cuts at: 2067 3171 4148 2067
Size: 1104 977 2634
Fragments arranged by size:
2634 1104 977
AgeI A'CCGG_T
Cuts at: 3103 3103
Size: 4715
AlwNI CAG_nnn'CTG
Cuts at: 2020 3587 2020
Size: 1567 3148
ApaLI G'TGCA_C
Cuts at: 3485 3485
Size: 4715
ApoI r'AATT_y
Cuts at: 134 1066 1675 1719 4068 4324 134
Size: 932 609 44 2349 256 525
Fragments arranged by size:
2349 932 609 525 256 44
AseI AT'TA_AT
Cuts at: 20 124 367 1298 20
Size: 104 243 931 3437
Fragments arranged by size:
3437 931 243 104
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
AvaI C'yCGr_G
Cuts at: 1735 1735
Size: 4715
BamHI G'GATC_C
Cuts at: 1740 1740
Size: 4715
BanI G'GyrC_C
Cuts at: 1731 2145 2393 2428 1731
Size: 414 248 35 4018
Fragments arranged by size:
4018 414 248 35
BanII G_rGCy'C
Cuts at: 1729 2759 1729
Size: 1030 3685
BbeI G_GCGC'C
Cuts at: 2149 2397 2149
Size: 248 4467
BbsI GAAGACnn'nnnn_
Cuts at: 1689 1689
Size: 4715
BbvCI CC'TCA_GC
Cuts at: 599 1186 599
Size: 587 4128
BciVI GTATCCnnnnn_n'
Cuts at: 516 531 1653 2613 3374 4715 516
Size: 15 1122 960 761 1341 516
Fragments arranged by size:
1341 1122 960 761 516 15
BclI T'GATC_A
Cuts at: 909 2235 909
Size: 1326 3389
BfrBI ATG'CAT
Cuts at: 358 358
Size: 4715
BglII A'GATC_T
Cuts at: 216 1314 2230 4267 216
Size: 1098 916 2037 664
Fragments arranged by size:
2037 1098 916 664
BlpI GC'TnA_GC
Cuts at: 1827 1827
Size: 4715
Bme1580I G_kGCm'C
Cuts at: 2340 2433 3489 2340
Size: 93 1056 3566
Fragments arranged by size:
3566 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 141 1927 2008 2343 4487 141
Size: 1786 81 335 2144 369
Fragments arranged by size:
2144 1786 369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 4715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1064 502
Size: 562 4153
Bpu10I CC'TnA_GC
Cuts at: 599 1186 599
Size: 587 4128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1891 2770 3262 3560 1891
Size: 879 492 298 3046
Fragments arranged by size:
3046 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 758 4419 758
Size: 3661 1054
BsaAI yAC'GTr
Cuts at: 2068 2068
Size: 4715
BsaBI GATnn'nnATC
Cuts at: 6 482 2229 4266 6
Size: 476 1747 2037 455
Fragments arranged by size:
2037 1747 476 455
BsaHI Gr'CG_yC
Cuts at: 2146 2394 3096 4592 2146
Size: 248 702 1496 2269
Fragments arranged by size:
2269 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1904 2425 3103 3377 3524 3935 1904
Size: 521 678 274 147 411 2684
Fragments arranged by size:
2684 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1490 1514 1520 1544 1490
Size: 24 6 24 4661
Fragments arranged by size:
4661 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1511 1511
Size: 4715
BseYI C'CCAG_C
Cuts at: 1437 2140 2898 3475 3971 1437
Size: 703 758 577 496 2181
Fragments arranged by size:
2181 758 703 577 496
BsiEI CG_ry'CG
Cuts at: 104 2257 2303 3511 104
Size: 2153 46 1208 1308
Fragments arranged by size:
2153 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1729 2507 2697 3489 1729
Size: 778 190 792 2955
Fragments arranged by size:
2955 792 778 190
BsmI GAATG_Cn'
Cuts at: 358 358
Size: 4715
BsmBI CGTCTCn'nnnn_
Cuts at: 520 1222 520
Size: 702 4013
Bsp1286I G_dGCh'C
Cuts at: 1729 2340 2433 2507 2697 2759 3489 1729
Size: 611 93 74 190 62 730 2955
Fragments arranged by size:
2955 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3935 3935
Size: 4715
BspHI T'CATG_A
Cuts at: 3891 3891
Size: 4715
BspMI ACCTGCnnnn'nnnn_
Cuts at: 962 1765 1979 2281 2662 962
Size: 803 214 302 381 3015
Fragments arranged by size:
3015 803 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3007 3061 4545 4710 50
Size: 2957 54 1484 165 55
Fragments arranged by size:
2957 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2627 2627
Size: 4715
BsrFI r'CCGG_y
Cuts at: 2713 2894 3103 2713
Size: 181 209 4325
Fragments arranged by size:
4325 209 181
BssSI C'ACGA_G
Cuts at: 2986 3344 2986
Size: 358 4357
BstAPI GCAn_nnn'nTGC
Cuts at: 2092 2092
Size: 4715
BstBI TT'CG_AA
Cuts at: 1680 3076 1680
Size: 1396 3319
BstXI CCAn_nnnn'nTGG
Cuts at: 1666 1666
Size: 4715
BstYI r'GATC_y
Cuts at: 1 216 324 810 1314 1740 2207 2230 2565
Size: 215 108 486 504 426 467 23 335
Cuts at: 2565 2811 3823 3909 3921 4267 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 504 486 467 449 426 346 335
246 215 108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 679 679
Size: 4715
BtgI C'CryG_G
Cuts at: 987 987
Size: 4715
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2665 2846 3009 2665
Size: 181 163 4371
Fragments arranged by size:
4371 181 163
BtsI GCAGTG_nn'
Cuts at: 1958 1958
Size: 4715
DraI TTT'AAA
Cuts at: 1714 1714
Size: 4715
DrdI GACnn_nn'nnGTC
Cuts at: 2421 3279 2421
Size: 858 3857
EaeI y'GGCC_r
Cuts at: 2300 2474 2865 2892 4237 2300
Size: 174 391 27 1345 2778
Fragments arranged by size:
2778 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2300 2300
Size: 4715
EarI CTCTTCn'nnn_
Cuts at: 248 2738 2948 248
Size: 2490 210 2015
Fragments arranged by size:
2490 2015 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2923 3233 3379 4525 2923
Size: 310 146 1146 3113
Fragments arranged by size:
3113 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1727 1727
Size: 4715
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 878 1064 1535 2540 2972 3719 3987 502
Size: 376 186 471 1005 432 747 268 1230
Fragments arranged by size:
1230 1005 747 471 432 376 268 186
EcoNI CCTnn'n_nnAGG
Cuts at: 772 772
Size: 4715
EcoO109I rG'GnC_Cy
Cuts at: 1854 1854
Size: 4715
EcoRI G'AATT_C
Cuts at: 134 1719 134
Size: 1585 3130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4715
FspI TGC'GCA
Cuts at: 2496 2496
Size: 4715
HaeII r_GCGC'y
Cuts at: 2149 2397 3419 4398 2149
Size: 248 1022 979 2466
Fragments arranged by size:
2466 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 309 341 535 567 706 738 889 921 2557
Size: 32 194 32 139 32 151 32 1636
Cuts at: 2557 2587 2589 2619 309
Size: 30 2 30 2405
Fragments arranged by size:
2405 1636 194 151 139 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1754 1754
Size: 4715
HindIII A'AGCT_T
Cuts at: 796 796
Size: 4715
KasI G'GCGC_C
Cuts at: 2145 2393 2145
Size: 248 4467
KpnI G_GTAC'C
Cuts at: 1735 1735
Size: 4715
MfeI C'AATT_G
Cuts at: 179 179
Size: 4715
MluI A'CGCG_T
Cuts at: 4148 4148
Size: 4715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 380 1353 1500 3386 3570 380
Size: 973 147 1886 184 1525
Fragments arranged by size:
1886 1525 973 184 147
MscI TGG'CCA
Cuts at: 2476 2476
Size: 4715
MslI CAynn'nnrTG
Cuts at: 1664 2704 2831 1664
Size: 1040 127 3548
Fragments arranged by size:
3548 1040 127
MspA1I CmG'CkG
Cuts at: 1826 2140 2500 3513 3758 1826
Size: 314 360 1013 245 2783
Fragments arranged by size:
2783 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2896 2896
Size: 4715
NarI GG'CG_CC
Cuts at: 2146 2394 2146
Size: 248 4467
NgoMIV G'CCGG_C
Cuts at: 2894 2894
Size: 4715
NsiI A_TGCA'T
Cuts at: 360 360
Size: 4715
NspI r_CATG'y
Cuts at: 1609 1768 2268 3175 1609
Size: 159 500 907 3149
Fragments arranged by size:
3149 907 500 159
PciI A'CATG_T
Cuts at: 3171 3171
Size: 4715
PflMI CCAn_nnn'nTGG
Cuts at: 113 153 1652 113
Size: 40 1499 3176
Fragments arranged by size:
3176 1499 40
PfoI T'CCnGG_A
Cuts at: 518 567 518
Size: 49 4666
PmeI GTTT'AAAC
Cuts at: 1714 1714
Size: 4715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 4683
PstI C_TGCA'G
Cuts at: 1494 1762 2447 1494
Size: 268 685 3762
Fragments arranged by size:
3762 685 268
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4715
PvuII CAG'CTG
Cuts at: 2140 2500 2140
Size: 360 4355
RsrII CG'GwC_CG
Cuts at: 2910 2910
Size: 4715
SacI G_AGCT'C
Cuts at: 1729 1729
Size: 4715
SalI G'TCGA_C
Cuts at: 1752 1752
Size: 4715
SapI GCTCTTCn'nnn_
Cuts at: 2738 2948 2738
Size: 210 4505
SbfI CC_TGCA'GG
Cuts at: 1762 1762
Size: 4715
ScaI AGT'ACT
Cuts at: 466 4232 466
Size: 3766 949
SfcI C'TryA_G
Cuts at: 33 1490 1758 2443 3436 3627 33
Size: 1457 268 685 993 191 1121
Fragments arranged by size:
1457 1121 993 685 268 191
SfoI GGC'GCC
Cuts at: 2147 2395 2147
Size: 248 4467
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4715
SmaI CCC'GGG
Cuts at: 1737 1737
Size: 4715
SmlI C'TyrA_G
Cuts at: 415 1870 2785 3277 3539 415
Size: 1455 915 492 262 1591
Fragments arranged by size:
1591 1455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 307 67
Size: 240 4475
SphI G_CATG'C
Cuts at: 1609 1768 2268 1609
Size: 159 500 4056
Fragments arranged by size:
4056 500 159
SspI AAT'ATT
Cuts at: 847 847
Size: 4715
StyI C'CwwG_G
Cuts at: 1180 1849 2203 1180
Size: 669 354 3692
Fragments arranged by size:
3692 669 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2298 2431 2890 3099 2298
Size: 133 459 209 3914
Fragments arranged by size:
3914 459 209 133
TatI w'GTAC_w
Cuts at: 310 464 1597 4230 310
Size: 154 1133 2633 795
Fragments arranged by size:
2633 1133 795 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1494 1526 4232 4264 1494
Size: 32 2706 32 1945
Fragments arranged by size:
2706 1945 32 32
Tth111I GACn'n_nGTC
Cuts at: 2512 2512
Size: 4715
XbaI T'CTAG_A
Cuts at: 729 1746 729
Size: 1017 3698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2210 2210
Size: 4715
XmaI C'CCGG_G
Cuts at: 1735 1735
Size: 4715
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BglI BmgBI BmtI BsgI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI