Restriction map for
pF1KB4964
(Circular) (Six-base) MAPSORT of: pF1KB4964.seq Check: 239 from: 1 to: 7505
pF1KB4964 7505 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AatII G_ACGT'C
Cuts at: 532 532
Size: 7505
AccI GT'mk_AC
Cuts at: 532 3905 3995 4543 532
Size: 3373 90 548 3494
Fragments arranged by size:
3494 3373 548 90
Acc65I G'GTAC_C
Cuts at: 4521 4521
Size: 7505
AclI AA'CG_TT
Cuts at: 1659 7345 1659
Size: 5686 1819
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 784 1519 1685 1827 2386 2431 3853 5330
Size: 180 735 166 142 559 45 1422 1477
Cuts at: 5330 5762 6509 6777 604
Size: 432 747 268 1332
Fragments arranged by size:
1477 1422 1332 747 735 559 432 268
180 166 142 45
AflIII A'CryG_T
Cuts at: 362 1390 2786 4461 4857 5961 6938 362
Size: 1028 1396 1675 396 1104 977 929
Fragments arranged by size:
1675 1396 1104 1028 977 929 396
AgeI A'CCGG_T
Cuts at: 2383 5893 2383
Size: 3510 3995
AhdI GACnn_n'nnGTC
Cuts at: 375 2946 375
Size: 2571 4934
AleI CACnn'nnGTG
Cuts at: 361 2659 361
Size: 2298 5207
AlwNI CAG_nnn'CTG
Cuts at: 444 3755 4810 6377 444
Size: 3311 1055 1567 1572
Fragments arranged by size:
3311 1572 1567 1055
ApaI G_GGCC'C
Cuts at: 277 277
Size: 7505
ApaLI G'TGCA_C
Cuts at: 1177 2728 6275 1177
Size: 1551 3547 2407
Fragments arranged by size:
3547 2407 1551
ApoI r'AATT_y
Cuts at: 652 2077 2227 2925 4074 4443 4509 6858 7114
Size: 1425 150 698 1149 369 66 2349 256
Cuts at: 7114 652
Size: 1043
Fragments arranged by size:
2349 1425 1149 1043 698 369 256 150
66
AseI AT'TA_AT
Cuts at: 20 20
Size: 7505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7505
AvaI C'yCGr_G
Cuts at: 277 1048 3532 4116 4525 277
Size: 771 2484 584 409 3257
Fragments arranged by size:
3257 2484 771 584 409
AvrII C'CTAG_G
Cuts at: 3253 4003 3253
Size: 750 6755
BamHI G'GATC_C
Cuts at: 522 2773 3564 4530 522
Size: 2251 791 966 3497
Fragments arranged by size:
3497 2251 966 791
BanI G'GyrC_C
Cuts at: 171 1732 4521 4935 5183 5218 171
Size: 1561 2789 414 248 35 2458
Fragments arranged by size:
2789 2458 1561 414 248 35
BanII G_rGCy'C
Cuts at: 277 908 3253 4519 5549 277
Size: 631 2345 1266 1030 2233
Fragments arranged by size:
2345 2233 1266 1030 631
BbeI G_GCGC'C
Cuts at: 175 4939 5187 175
Size: 4764 248 2493
Fragments arranged by size:
4764 2493 248
BbsI GAAGACnn'nnnn_
Cuts at: 606 1373 3863 606
Size: 767 2490 4248
Fragments arranged by size:
4248 2490 767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1619 1653 1619
Size: 34 7471
BciVI GTATCCnnnnn_n'
Cuts at: 1849 5403 6164 7505 1849
Size: 3554 761 1341 1849
Fragments arranged by size:
3554 1849 1341 761
BclI T'GATC_A
Cuts at: 506 716 5025 506
Size: 210 4309 2986
Fragments arranged by size:
4309 2986 210
BfrBI ATG'CAT
Cuts at: 2211 2211
Size: 7505
BglI GCCn_nnn'nGGC
Cuts at: 2053 2590 2053
Size: 537 6968
BglII A'GATC_T
Cuts at: 3455 5020 7057 3455
Size: 1565 2037 3903
Fragments arranged by size:
3903 2037 1565
BlpI GC'TnA_GC
Cuts at: 1255 4617 1255
Size: 3362 4143
Bme1580I G_kGCm'C
Cuts at: 277 387 1181 1688 2732 3924 5130 5223 6279
Size: 110 794 507 1044 1192 1206 93 1056
Cuts at: 6279 277
Size: 1503
Fragments arranged by size:
1503 1206 1192 1056 1044 794 507 110
93
BmgBI CAC'GTC
Cuts at: 243 306 1391 243
Size: 63 1085 6357
Fragments arranged by size:
6357 1085 63
BmrI ACTGGGnnnn_n'
Cuts at: 966 2507 3501 3563 3927 4717 4798 5133 7277
Size: 1541 994 62 364 790 81 335 2144
Cuts at: 7277 966
Size: 1194
Fragments arranged by size:
2144 1541 1194 994 790 364 335 81
62
BmtI G_CTAG'C
Cuts at: 2039 2039
Size: 7505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 855 1053 2911 4190 5872 855
Size: 198 1858 1279 1682 2488
Fragments arranged by size:
2488 1858 1682 1279 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 991 1048 1360 2500 2738 4087 4171 991
Size: 57 312 1140 238 1349 84 4325
Fragments arranged by size:
4325 1349 1140 312 238 84 57
Bpu10I CC'TnA_GC
Cuts at: 2596 3958 4018 2596
Size: 1362 60 6083
Fragments arranged by size:
6083 1362 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2829 4030 4089 4341 4681 5560 6052 6350 2829
Size: 1201 59 252 340 879 492 298 3984
Fragments arranged by size:
3984 1201 879 492 340 298 252 59
BsaI GGTCTCn'nnnn_
Cuts at: 894 2115 2476 3706 7209 894
Size: 1221 361 1230 3503 1190
Fragments arranged by size:
3503 1230 1221 1190 361
BsaAI yAC'GTr
Cuts at: 363 3314 4858 363
Size: 2951 1544 3010
Fragments arranged by size:
3010 2951 1544
BsaBI GATnn'nnATC
Cuts at: 6 5019 7056 6
Size: 5013 2037 455
Fragments arranged by size:
5013 2037 455
BsaHI Gr'CG_yC
Cuts at: 172 529 2252 4936 5184 5886 7382 172
Size: 357 1723 2684 248 702 1496 295
Fragments arranged by size:
2684 1723 1496 702 357 295 248
BsaWI w'CCGG_w
Cuts at: 2383 2801 3611 4694 5215 5893 6167 6314 6725
Size: 418 810 1083 521 678 274 147 411
Cuts at: 6725 2383
Size: 3163
Fragments arranged by size:
3163 1083 810 678 521 418 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2473 2503 2824 2854 4177 4207 2473
Size: 30 321 30 1323 30 5771
Fragments arranged by size:
5771 1323 321 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 447 1357 2488 2788 2832 4179 447
Size: 910 1131 300 44 1347 3773
Fragments arranged by size:
3773 1347 1131 910 300 44
BseYI C'CCAG_C
Cuts at: 225 698 880 2323 3885 4930 5688 6265 6761
Size: 473 182 1443 1562 1045 758 577 496
Cuts at: 6761 225
Size: 969
Fragments arranged by size:
1562 1443 1045 969 758 577 496 473
182
BsiEI CG_ry'CG
Cuts at: 104 2673 4315 5047 5093 6301 104
Size: 2569 1642 732 46 1208 1308
Fragments arranged by size:
2569 1642 1308 1208 732 46
BsiHKAI G_wGCw'C
Cuts at: 908 1181 2624 2668 2732 3112 4519 5297 5487
Size: 273 1443 44 64 380 1407 778 190
Cuts at: 5487 6279 908
Size: 792 2134
Fragments arranged by size:
2134 1443 1407 792 778 380 273 190
64 44
BsmI GAATG_Cn'
Cuts at: 485 2717 3975 485
Size: 2232 1258 4015
Fragments arranged by size:
4015 2232 1258
BsmBI CGTCTCn'nnnn_
Cuts at: 1381 1743 1381
Size: 362 7143
Bsp1286I G_dGCh'C
Cuts at: 277 387 908 1181 1688 2624 2668 2732 3112
Size: 110 521 273 507 936 44 64 380
Cuts at: 3112 3253 3924 4519 5130 5223 5297 5487 5549
Size: 141 671 595 611 93 74 190 62
Cuts at: 5549 6279 277
Size: 730 1503
Fragments arranged by size:
1503 936 730 671 611 595 521 507
380 273 190 141 110 93 74 64
62 44
BspEI T'CCGG_A
Cuts at: 6725 6725
Size: 7505
BspHI T'CATG_A
Cuts at: 2063 2127 6681 2063
Size: 64 4554 2887
Fragments arranged by size:
4554 2887 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3891 4555 4769 5071 5452 3891
Size: 664 214 302 381 5944
Fragments arranged by size:
5944 664 381 302 214
BsrBI CCG'CTC
Cuts at: 50 839 5797 5851 7335 7500 50
Size: 789 4958 54 1484 165 55
Fragments arranged by size:
4958 1484 789 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5417 5417
Size: 7505
BsrFI r'CCGG_y
Cuts at: 144 2095 2383 2672 3014 3728 5503 5684 5893
Size: 1951 288 289 342 714 1775 181 209
Cuts at: 5893 144
Size: 1756
Fragments arranged by size:
1951 1775 1756 714 342 289 288 209
181
BsrGI T'GTAC_A
Cuts at: 4466 4466
Size: 7505
BssSI C'ACGA_G
Cuts at: 400 5776 6134 400
Size: 5376 358 1771
Fragments arranged by size:
5376 1771 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1107 3755 4392 4882 1107
Size: 2648 637 490 3730
Fragments arranged by size:
3730 2648 637 490
BstBI TT'CG_AA
Cuts at: 261 5866 261
Size: 5605 1900
BstEII G'GTnAC_C
Cuts at: 624 1545 2349 3880 624
Size: 921 804 1531 4249
Fragments arranged by size:
4249 1531 921 804
BstXI CCAn_nnnn'nTGG
Cuts at: 1191 1191
Size: 7505
BstYI r'GATC_y
Cuts at: 1 522 770 2501 2773 3455 3498 3564 4530
Size: 521 248 1731 272 682 43 66 966
Cuts at: 4530 4997 5020 5355 5601 6613 6699 6711 7057
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7057 1
Size: 449
Fragments arranged by size:
1731 1012 966 682 521 467 449 346
335 272 248 246 86 66 43 23
12
Bsu36I CC'TnA_GG
Cuts at: 1261 1261
Size: 7505
BtgI C'CryG_G
Cuts at: 107 393 572 1890 2588 3158 107
Size: 286 179 1318 698 570 4454
Fragments arranged by size:
4454 1318 698 570 286 179
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3830 5455 5636 5799 3830
Size: 1625 181 163 5536
Fragments arranged by size:
5536 1625 181 163
BtsI GCAGTG_nn'
Cuts at: 3744 4748 3744
Size: 1004 6501
ClaI AT'CG_AT
Cuts at: 3851 3851
Size: 7505
DraI TTT'AAA
Cuts at: 3366 4442 4504 3366
Size: 1076 62 6367
Fragments arranged by size:
6367 1076 62
DraIII CAC_nnn'GTG
Cuts at: 243 1272 243
Size: 1029 6476
DrdI GACnn_nn'nnGTC
Cuts at: 610 2257 5211 6069 610
Size: 1647 2954 858 2046
Fragments arranged by size:
2954 2046 1647 858
EaeI y'GGCC_r
Cuts at: 3008 3101 5090 5264 5655 5682 7027 3008
Size: 93 1989 174 391 27 1345 3486
Fragments arranged by size:
3486 1989 1345 391 174 93 27
EagI C'GGCC_G
Cuts at: 5090 5090
Size: 7505
EarI CTCTTCn'nnn_
Cuts at: 783 2416 2632 2692 3263 3626 5528 5738 783
Size: 1633 216 60 571 363 1902 210 2550
Fragments arranged by size:
2550 1902 1633 571 363 216 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 202 5713 6023 6169 7315 202
Size: 5511 310 146 1146 392
Fragments arranged by size:
5511 1146 392 310 146
EcoICRI GAG'CTC
Cuts at: 906 4517 906
Size: 3611 3894
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 784 991 1048 1360 1519 1685 1827 2386
Size: 180 207 57 312 159 166 142 559
Cuts at: 2386 2431 2500 2738 3853 4087 4171 5330 5762
Size: 45 69 238 1115 234 84 1159 432
Cuts at: 5762 6509 6777 604
Size: 747 268 1332
Fragments arranged by size:
1332 1159 1115 747 559 432 312 268
238 234 207 180 166 159 142 84
69 57 45
EcoNI CCTnn'n_nnAGG
Cuts at: 1342 1342
Size: 7505
EcoO109I rG'GnC_Cy
Cuts at: 196 273 274 1092 1347 1935 2948 3233 3800
Size: 77 1 818 255 588 1013 285 567
Cuts at: 3800 4025 4644 196
Size: 225 619 3057
Fragments arranged by size:
3057 1013 818 619 588 567 285 255
225 77 1
EcoRI G'AATT_C
Cuts at: 652 4509 652
Size: 3857 3648
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2661 3378 3655 4194 5524 2661
Size: 717 277 539 1330 4642
Fragments arranged by size:
4642 1330 717 539 277
FspI TGC'GCA
Cuts at: 5286 5286
Size: 7505
HaeII r_GCGC'y
Cuts at: 175 1772 4939 5187 6209 7188 175
Size: 1597 3167 248 1022 979 492
Fragments arranged by size:
3167 1597 1022 979 492 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 213 245 361 393 439 471 514 542 546
Size: 32 116 32 46 32 43 28 4
Cuts at: 546 574 910 942 2932 2964 3169 3201 3440
Size: 28 336 32 1990 32 205 32 239
Cuts at: 3440 3472 3954 3986 4177 4209 5347 5377 5379
Size: 32 482 32 191 32 1138 30 2
Cuts at: 5379 5409 213
Size: 30 2309
Fragments arranged by size:
2309 1990 1138 482 336 239 205 191
116 46 43 32 32 32 32 32
32 32 32 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 1575 3996 4328 4544 1575
Size: 2421 332 216 4536
Fragments arranged by size:
4536 2421 332 216
HindIII A'AGCT_T
Cuts at: 1504 1504
Size: 7505
HpaI GTT'AAC
Cuts at: 1575 1575
Size: 7505
KasI G'GCGC_C
Cuts at: 171 4935 5183 171
Size: 4764 248 2493
Fragments arranged by size:
4764 2493 248
KpnI G_GTAC'C
Cuts at: 4525 4525
Size: 7505
MfeI C'AATT_G
Cuts at: 1907 1907
Size: 7505
MluI A'CGCG_T
Cuts at: 4461 6938 4461
Size: 2477 5028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 550 1322 1396 1833 2693 2855 3104 3668 3788
Size: 772 74 437 860 162 249 564 120
Cuts at: 3788 6176 6360 550
Size: 2388 184 1695
Fragments arranged by size:
2388 1695 860 772 564 437 249 184
162 120 74
MscI TGG'CCA
Cuts at: 3010 5266 3010
Size: 2256 5249
MslI CAynn'nnrTG
Cuts at: 361 577 2659 2905 4223 4367 5494 5621 361
Size: 216 2082 246 1318 144 1127 127 2245
Fragments arranged by size:
2245 2082 1318 1127 246 216 144 127
MspA1I CmG'CkG
Cuts at: 762 1012 1064 2541 2714 3144 4616 4930 5290
Size: 250 52 1477 173 430 1472 314 360
Cuts at: 5290 6303 6548 762
Size: 1013 245 1719
Fragments arranged by size:
1719 1477 1472 1013 430 360 314 250
245 173 52
NaeI GCC'GGC
Cuts at: 146 5686 146
Size: 5540 1965
NarI GG'CG_CC
Cuts at: 172 4936 5184 172
Size: 4764 248 2493
Fragments arranged by size:
4764 2493 248
NcoI C'CATG_G
Cuts at: 107 1890 2588 107
Size: 1783 698 5024
Fragments arranged by size:
5024 1783 698
NgoMIV G'CCGG_C
Cuts at: 144 5684 144
Size: 5540 1965
NheI G'CTAG_C
Cuts at: 2035 2035
Size: 7505
NsiI A_TGCA'T
Cuts at: 2213 2213
Size: 7505
NspI r_CATG'y
Cuts at: 613 708 2211 2577 2790 2829 3771 4390 4558
Size: 95 1503 366 213 39 942 619 168
Cuts at: 4558 5058 5965 613
Size: 500 907 2153
Fragments arranged by size:
2153 1503 942 907 619 500 366 213
168 95 39
PasI CC'CwG_GG
Cuts at: 4433 4433
Size: 7505
PciI A'CATG_T
Cuts at: 2786 5961 2786
Size: 3175 4330
PflMI CCAn_nnn'nTGG
Cuts at: 444 4149 4236 444
Size: 3705 87 3713
Fragments arranged by size:
3713 3705 87
PfoI T'CCnGG_A
Cuts at: 3548 4030 3548
Size: 482 7023
PmeI GTTT'AAAC
Cuts at: 4504 4504
Size: 7505
PmlI CAC'GTG
Cuts at: 363 3314 363
Size: 2951 4554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6644 6676 6644
Size: 32 7473
PpuMI rG'GwC_Cy
Cuts at: 196 1092 2948 3233 3800 196
Size: 896 1856 285 567 3901
Fragments arranged by size:
3901 1856 896 567 285
PshAI GACnn'nnGTC
Cuts at: 754 754
Size: 7505
PspOMI G'GGCC_C
Cuts at: 273 273
Size: 7505
PstI C_TGCA'G
Cuts at: 3751 4453 4552 5237 3751
Size: 702 99 685 6019
Fragments arranged by size:
6019 702 685 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7505
PvuII CAG'CTG
Cuts at: 762 1064 2541 2714 3144 4930 5290 762
Size: 302 1477 173 430 1786 360 2977
Fragments arranged by size:
2977 1786 1477 430 360 302 173
RsrII CG'GwC_CG
Cuts at: 166 5700 166
Size: 5534 1971
SacI G_AGCT'C
Cuts at: 908 4519 908
Size: 3611 3894
SalI G'TCGA_C
Cuts at: 3994 4542 3994
Size: 548 6957
SanDI GG'GwC_CC
Cuts at: 196 196
Size: 7505
SapI GCTCTTCn'nnn_
Cuts at: 2692 5528 5738 2692
Size: 2836 210 4459
Fragments arranged by size:
4459 2836 210
SbfI CC_TGCA'GG
Cuts at: 4552 4552
Size: 7505
ScaI AGT'ACT
Cuts at: 7022 7022
Size: 7505
SexAI A'CCwGG_T
Cuts at: 1172 1172
Size: 7505
SfcI C'TryA_G
Cuts at: 33 645 963 2709 3399 3464 3747 4449 4548
Size: 612 318 1746 690 65 283 702 99
Cuts at: 4548 5233 6226 6417 33
Size: 685 993 191 1121
Fragments arranged by size:
1746 1121 993 702 690 685 612 318
283 191 99 65
SfoI GGC'GCC
Cuts at: 173 4937 5185 173
Size: 4764 248 2493
Fragments arranged by size:
4764 2493 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7505
SmaI CCC'GGG
Cuts at: 4527 4527
Size: 7505
SmlI C'TyrA_G
Cuts at: 2844 4045 4104 4320 4660 5575 6067 6329 2844
Size: 1201 59 216 340 915 492 262 4020
Fragments arranged by size:
4020 1201 915 492 340 262 216 59
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7505
SphI G_CATG'C
Cuts at: 4558 5058 4558
Size: 500 7005
SspI AAT'ATT
Cuts at: 213 213
Size: 7505
StyI C'CwwG_G
Cuts at: 107 843 1055 1890 2255 2416 2588 3253 3353
Size: 736 212 835 365 161 172 665 100
Cuts at: 3353 3392 4003 4143 4639 4993 107
Size: 39 611 140 496 354 2619
Fragments arranged by size:
2619 835 736 665 611 496 365 354
212 172 161 140 100 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1127 1770 2446 2874 4301 5088 5221 5680 5889
Size: 643 676 428 1427 787 133 459 209
Cuts at: 5889 1127
Size: 2743
Fragments arranged by size:
2743 1427 787 676 643 459 428 209
133
TatI w'GTAC_w
Cuts at: 2216 2933 4421 4466 7020 2216
Size: 717 1488 45 2554 2701
Fragments arranged by size:
2701 2554 1488 717 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2473 2505 7022 7054 2473
Size: 32 4517 32 2924
Fragments arranged by size:
4517 2924 32 32
Tth111I GACn'n_nGTC
Cuts at: 5302 5302
Size: 7505
XbaI T'CTAG_A
Cuts at: 1755 3130 4536 1755
Size: 1375 1406 4724
Fragments arranged by size:
4724 1406 1375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1992 5000 114
Size: 1878 3008 2619
Fragments arranged by size:
3008 2619 1878
XmaI C'CCGG_G
Cuts at: 4525 4525
Size: 7505
XmnI GAAnn'nnTTC
Cuts at: 4061 4061
Size: 7505
ZraI GAC'GTC
Cuts at: 530 530
Size: 7505
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI AscI BaeI BbvCI
BsgI BsiWI BssHII BstZ17I CspCI EcoRV FseI FspAI
NdeI NotI NruI PacI PsiI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI