Restriction map for
pF1KB4967
(Circular) (Six-base) MAPSORT of: pF1KB4967.seq Check: 4886 from: 1 to: 10355
pF1KB4967 10355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5122 5197 5883 6856 5122
Size: 75 686 973 8621
Fragments arranged by size:
8621 973 686 75
AccI GT'mk_AC
Cuts at: 3521 4286 5108 6876 7393 3521
Size: 765 822 1768 517 6483
Fragments arranged by size:
6483 1768 822 765 517
Acc65I G'GTAC_C
Cuts at: 7371 7371
Size: 10355
AclI AA'CG_TT
Cuts at: 10195 10195
Size: 10355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 283 760 2290 3188 4601 6886 8180 8612
Size: 158 477 1530 898 1413 2285 1294 432
Cuts at: 8612 8965 9627 125
Size: 353 662 853
Fragments arranged by size:
2285 1530 1413 1294 898 853 662 477
432 353 158
AfeI AGC'GCT
Cuts at: 5317 5317
Size: 10355
AflII C'TTAA_G
Cuts at: 832 832
Size: 10355
AflIII A'CryG_T
Cuts at: 1961 7347 7707 9507 9788 1961
Size: 5386 360 1800 281 2528
Fragments arranged by size:
5386 2528 1800 360 281
AhdI GACnn_n'nnGTC
Cuts at: 6029 6029
Size: 10355
AleI CACnn'nnGTG
Cuts at: 2989 4723 2989
Size: 1734 8621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 5480 6389 5480
Size: 909 9446
AlwNI CAG_nnn'CTG
Cuts at: 991 2692 2875 3279 3630 5331 6241 7660 9098
Size: 1701 183 404 351 1701 910 1419 1438
Cuts at: 9098 991
Size: 2248
Fragments arranged by size:
2248 1701 1701 1438 1419 910 404 351
183
ApaI G_GGCC'C
Cuts at: 5870 5870
Size: 10355
ApaLI G'TGCA_C
Cuts at: 3654 3944 9193 3654
Size: 290 5249 4816
Fragments arranged by size:
5249 4816 290
ApoI r'AATT_y
Cuts at: 1719 1805 2595 2994 5945 7215 7314 7359 9708
Size: 86 790 399 2951 1270 99 45 2349
Cuts at: 9708 9964 1719
Size: 256 2110
Fragments arranged by size:
2951 2349 2110 1270 790 399 256 99
86 45
AseI AT'TA_AT
Cuts at: 20 247 290 20
Size: 227 43 10085
Fragments arranged by size:
10085 227 43
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10355
AvaI C'yCGr_G
Cuts at: 4819 7375 4819
Size: 2556 7799
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3582 3615 5166 5199 3582
Size: 33 1551 33 8738
Fragments arranged by size:
8738 1551 33 33
BamHI G'GATC_C
Cuts at: 1893 3312 3825 7380 1893
Size: 1419 513 3555 4868
Fragments arranged by size:
4868 3555 1419 513
BanI G'GyrC_C
Cuts at: 4003 5441 5514 6868 7371 7785 8033 8068 4003
Size: 1438 73 1354 503 414 248 35 6290
Fragments arranged by size:
6290 1438 1354 503 414 248 73 35
BanII G_rGCy'C
Cuts at: 787 2340 2661 3394 3449 5049 5287 5870 6595
Size: 1553 321 733 55 1600 238 583 725
Cuts at: 6595 6946 7369 8399 787
Size: 351 423 1030 2743
Fragments arranged by size:
2743 1600 1553 1030 733 725 583 423
351 321 238 55
BbeI G_GCGC'C
Cuts at: 7789 8037 7789
Size: 248 10107
BbsI GAAGACnn'nnnn_
Cuts at: 1678 3122 3194 4881 1678
Size: 1444 72 1687 7152
Fragments arranged by size:
7152 1687 1444 72
BbvCI CC'TCA_GC
Cuts at: 3350 5732 6110 7106 3350
Size: 2382 378 996 6599
Fragments arranged by size:
6599 2382 996 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2830 2864 6054 6088 2830
Size: 34 3190 34 7097
Fragments arranged by size:
7097 3190 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3583 5320 6334 8253 9309 10355 3583
Size: 1737 1014 1919 1056 1046 3583
Fragments arranged by size:
3583 1919 1737 1056 1046 1014
BclI T'GATC_A
Cuts at: 7875 7875
Size: 10355
BfrBI ATG'CAT
Cuts at: 3870 4236 3870
Size: 366 9989
BglI GCCn_nnn'nGGC
Cuts at: 178 427 1259 2721 6432 178
Size: 249 832 1462 3711 4101
Fragments arranged by size:
4101 3711 1462 832 249
BglII A'GATC_T
Cuts at: 3875 7870 9907 3875
Size: 3995 2037 4323
Fragments arranged by size:
4323 3995 2037
BlpI GC'TnA_GC
Cuts at: 2660 5849 6602 7467 2660
Size: 3189 753 865 5548
Fragments arranged by size:
5548 3189 865 753
Bme1580I G_kGCm'C
Cuts at: 526 1760 3658 3948 5446 5870 7980 8073 9197
Size: 1234 1898 290 1498 424 2110 93 1124
Cuts at: 9197 526
Size: 1684
Fragments arranged by size:
2110 1898 1684 1498 1234 1124 424 290
93
BmrI ACTGGGnnnn_n'
Cuts at: 3207 3565 5043 6029 6969 7567 7648 7983 10127
Size: 358 1478 986 940 598 81 335 2144
Cuts at: 10127 3207
Size: 3435
Fragments arranged by size:
3435 2144 1478 986 940 598 358 335
81
BmtI G_CTAG'C
Cuts at: 7241 7241
Size: 10355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4269 8722 4269
Size: 4453 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1043 1103 1421 2354 2459 2519 3212 5044
Size: 136 60 318 933 105 60 693 1832
Cuts at: 5044 6111 6553 7065 907
Size: 1067 442 512 4197
Fragments arranged by size:
4197 1832 1067 933 693 512 442 318
136 105 60 60
Bpu10I CC'TnA_GC
Cuts at: 1465 3350 5732 6110 7106 1465
Size: 1885 2382 378 996 4714
Fragments arranged by size:
4714 2382 1885 996 378
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2468 4353 5762 6675 7531 8410 9124 9422 2468
Size: 1885 1409 913 856 879 714 298 3401
Fragments arranged by size:
3401 1885 1409 913 879 856 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 925 2546 6290 10059 925
Size: 1621 3744 3769 1221
Fragments arranged by size:
3769 3744 1621 1221
BsaAI yAC'GTr
Cuts at: 5940 7708 5940
Size: 1768 8587
BsaBI GATnn'nnATC
Cuts at: 6 290 4952 7869 9906 6
Size: 284 4662 2917 2037 455
Fragments arranged by size:
4662 2917 2037 455 284
BsaHI Gr'CG_yC
Cuts at: 181 5710 7786 8034 8736 10232 181
Size: 5529 2076 248 702 1496 304
Fragments arranged by size:
5529 2076 1496 702 304 248
BsaWI w'CCGG_w
Cuts at: 3503 7544 8065 8743 9154 9301 9575 3503
Size: 4041 521 678 411 147 274 4283
Fragments arranged by size:
4283 4041 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1394 1424 5630 5660 1394
Size: 30 4206 30 6089
Fragments arranged by size:
6089 4206 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2291 3196 4293 5828 2291
Size: 905 1097 1535 6818
Fragments arranged by size:
6818 1535 1097 905
BseYI C'CCAG_C
Cuts at: 824 1489 2270 2567 2837 2930 3767 5714 6116
Size: 665 781 297 270 93 837 1947 402
Cuts at: 6116 6164 6289 6341 6545 6947 7037 7067 7780
Size: 48 125 52 204 402 90 30 713
Cuts at: 7780 8538 9203 9611 824
Size: 758 665 408 1568
Fragments arranged by size:
1947 1568 837 781 758 713 665 665
408 402 402 297 270 204 125 93
90 52 48 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 2818 5065 5422 2801
Size: 17 2247 357 7734
Fragments arranged by size:
7734 2247 357 17
BsiEI CG_ry'CG
Cuts at: 104 7897 7943 9173 104
Size: 7793 46 1230 1286
Fragments arranged by size:
7793 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 230 1089 1407 2340 2866 3658 3948 7369 8147
Size: 859 318 933 526 792 290 3421 778
Cuts at: 8147 8337 9197 230
Size: 190 860 1388
Fragments arranged by size:
3421 1388 933 860 859 792 778 526
318 290 190
BsmI GAATG_Cn'
Cuts at: 621 1007 1707 5128 5361 6355 621
Size: 386 700 3421 233 994 4621
Fragments arranged by size:
4621 3421 994 700 386 233
BsmBI CGTCTCn'nnnn_
Cuts at: 5911 6651 5911
Size: 740 9615
Bsp1286I G_dGCh'C
Cuts at: 230 526 787 1089 1407 1760 2340 2661 2866
Size: 296 261 302 318 353 580 321 205
Cuts at: 2866 3394 3449 3658 3948 5049 5287 5446 5870
Size: 528 55 209 290 1101 238 159 424
Cuts at: 5870 6595 6946 7369 7980 8073 8147 8337 8399
Size: 725 351 423 611 93 74 190 62
Cuts at: 8399 9197 230
Size: 798 1388
Fragments arranged by size:
1388 1101 798 725 611 580 528 424
423 353 351 321 318 302 296 290
261 238 209 205 190 159 93 74
62 55
BspHI T'CATG_A
Cuts at: 680 4350 8787 680
Size: 3670 4437 2248
Fragments arranged by size:
4437 3670 2248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1023 5122 5197 5883 6856 7405 7619 7921 8302
Size: 4099 75 686 973 549 214 302 381
Cuts at: 8302 1023
Size: 3076
Fragments arranged by size:
4099 3076 973 686 549 381 302 214
75
BsrBI CCG'CTC
Cuts at: 50 1987 8647 8701 10185 10350 50
Size: 1937 6660 54 1484 165 55
Fragments arranged by size:
6660 1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 657 3005 4452 4716 4907 5124 5462 5900 8267
Size: 2348 1447 264 191 217 338 438 2367
Cuts at: 8267 657
Size: 2745
Fragments arranged by size:
2745 2367 2348 1447 438 338 264 217
191
BsrFI r'CCGG_y
Cuts at: 8353 8534 8353
Size: 181 10174
BsrGI T'GTAC_A
Cuts at: 1324 3847 1324
Size: 2523 7832
BssSI C'ACGA_G
Cuts at: 3665 8626 9334 3665
Size: 4961 708 4686
Fragments arranged by size:
4961 4686 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1268 1285 2076 2824 5123 5559 6315 6672 6786
Size: 17 791 748 2299 436 756 357 114
Cuts at: 6786 6798 6807 6957 7732 1268
Size: 12 9 150 775 3891
Fragments arranged by size:
3891 2299 791 775 756 748 436 357
150 114 17 12 9
BstBI TT'CG_AA
Cuts at: 1845 8716 1845
Size: 6871 3484
BstEII G'GTnAC_C
Cuts at: 5820 5820
Size: 10355
BstXI CCAn_nnnn'nTGG
Cuts at: 4644 6757 7182 7284 4644
Size: 2113 425 102 7715
Fragments arranged by size:
7715 2113 425 102
BstYI r'GATC_y
Cuts at: 1 1839 1893 2024 3312 3399 3728 3825 3875
Size: 1838 54 131 1288 87 329 97 50
Cuts at: 3875 7001 7380 7847 7870 8205 8451 8757 8769
Size: 3126 379 467 23 335 246 306 12
Cuts at: 8769 8855 9907 1
Size: 86 1052 449
Fragments arranged by size:
3126 1838 1288 1052 467 449 379 335
329 306 246 131 97 87 86 54
50 23 12
BstZ17I GTA'TAC
Cuts at: 3522 4287 3522
Size: 765 9590
BtgI C'CryG_G
Cuts at: 107 593 2190 2313 3395 4103 4868 5990 5999
Size: 486 1597 123 1082 708 765 1122 9
Cuts at: 5999 6578 7054 7181 7263 7277 107
Size: 579 476 127 82 14 3185
Fragments arranged by size:
3185 1597 1122 1082 765 708 579 486
476 127 123 82 14 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 206 8305 8486 8649 206
Size: 8099 181 163 1912
Fragments arranged by size:
8099 1912 181 163
BtsI GCAGTG_nn'
Cuts at: 1201 1248 2202 5368 5490 5888 7598 1201
Size: 47 954 3166 122 398 1710 3958
Fragments arranged by size:
3958 3166 1710 954 398 122 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4118 4153 5747 5782 4118
Size: 35 1594 35 8691
Fragments arranged by size:
8691 1594 35 35
DraI TTT'AAA
Cuts at: 1907 3935 4569 7354 1907
Size: 2028 634 2785 4908
Fragments arranged by size:
4908 2785 2028 634
DraIII CAC_nnn'GTG
Cuts at: 1285 4429 5063 5136 1285
Size: 3144 634 73 6504
Fragments arranged by size:
6504 3144 634 73
DrdI GACnn_nn'nnGTC
Cuts at: 1337 5104 6282 8061 9405 1337
Size: 3767 1178 1779 1344 2287
Fragments arranged by size:
3767 2287 1779 1344 1178
EaeI y'GGCC_r
Cuts at: 110 419 2253 5603 5685 6722 7057 7940 8114
Size: 309 1834 3350 82 1037 335 883 174
Cuts at: 8114 8505 8532 9877 110
Size: 391 27 1345 588
Fragments arranged by size:
3350 1834 1345 1037 883 588 391 335
309 174 82 27
EagI C'GGCC_G
Cuts at: 7940 7940
Size: 10355
EarI CTCTTCn'nnn_
Cuts at: 779 2309 2591 3140 3382 4676 5037 8378 8588
Size: 1530 282 549 242 1294 361 3341 210
Cuts at: 8588 779
Size: 2546
Fragments arranged by size:
3341 2546 1530 1294 549 361 282 242
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 4891 8563 9305 9451 10165 174
Size: 4717 3672 742 146 714 364
Fragments arranged by size:
4717 3672 742 714 364 146
EcoICRI GAG'CTC
Cuts at: 2338 7367 2338
Size: 5029 5326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 283 760 907 1043 1103 1421 2290 2354
Size: 158 477 147 136 60 318 869 64
Cuts at: 2354 2459 2519 3188 3212 4601 5044 6111 6553
Size: 105 60 669 24 1389 443 1067 442
Cuts at: 6553 6886 7065 8180 8612 8965 9627 125
Size: 333 179 1115 432 353 662 853
Fragments arranged by size:
1389 1115 1067 869 853 669 662 477
443 442 432 353 333 318 179 158
147 136 105 64 60 60 24
EcoNI CCTnn'n_nnAGG
Cuts at: 449 995 4058 449
Size: 546 3063 6746
Fragments arranged by size:
6746 3063 546
EcoO109I rG'GnC_Cy
Cuts at: 544 820 1768 2122 5643 5866 5867 6031 6712
Size: 276 948 354 3521 223 1 164 681
Cuts at: 6712 6917 7494 544
Size: 205 577 3405
Fragments arranged by size:
3521 3405 948 681 577 354 276 223
205 164 1
EcoRI G'AATT_C
Cuts at: 1805 2595 2994 7215 7314 7359 1805
Size: 790 399 4221 99 45 4801
Fragments arranged by size:
4801 4221 790 399 99 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8374 8374
Size: 10355
FspI TGC'GCA
Cuts at: 8136 8136
Size: 10355
HaeII r_GCGC'y
Cuts at: 1167 5319 5928 7277 7789 8037 9267 10038 1167
Size: 4152 609 1349 512 248 1230 771 1484
Fragments arranged by size:
4152 1484 1349 1230 771 609 512 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 558 590 756 788 1477 1509 1626
Size: 32 333 32 166 32 689 32 117
Cuts at: 1626 1658 2202 2234 3304 3336 4968 5000 6015
Size: 32 544 32 1070 32 1632 32 1015
Cuts at: 6015 6047 6777 6809 7089 7121 8197 8227 8229
Size: 32 730 32 280 32 1076 30 2
Cuts at: 8229 8259 193
Size: 30 2289
Fragments arranged by size:
2289 1632 1076 1070 1015 730 689 544
333 280 166 117 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 557 1018 1447 3337 4215 6175 7394 557
Size: 461 429 1890 878 1960 1219 3518
Fragments arranged by size:
3518 1960 1890 1219 878 461 429
KasI G'GCGC_C
Cuts at: 7785 8033 7785
Size: 248 10107
KpnI G_GTAC'C
Cuts at: 7375 7375
Size: 10355
MfeI C'AATT_G
Cuts at: 692 5361 692
Size: 4669 5686
MluI A'CGCG_T
Cuts at: 9788 9788
Size: 10355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1392 2408 3590 5332 5695 5854 5988 9114 9298
Size: 1016 1182 1742 363 159 134 3126 184
Cuts at: 9298 1392
Size: 2449
Fragments arranged by size:
3126 2449 1742 1182 1016 363 184 159
134
MscI TGG'CCA
Cuts at: 421 2255 5605 5687 6724 8116 421
Size: 1834 3350 82 1037 1392 2660
Fragments arranged by size:
3350 2660 1834 1392 1037 82
MslI CAynn'nnrTG
Cuts at: 517 598 610 2989 3707 4723 5065 5071 6004
Size: 81 12 2379 718 1016 342 6 933
Cuts at: 6004 8344 8471 517
Size: 2340 127 2401
Fragments arranged by size:
2401 2379 2340 1016 933 718 342 127
81 12 6
MspA1I CmG'CkG
Cuts at: 348 1096 2465 2898 2938 5559 5616 5914 6345
Size: 748 1369 433 40 2621 57 298 431
Cuts at: 6345 6369 6783 7466 7780 8140 8924 9169 348
Size: 24 414 683 314 360 784 245 1534
Fragments arranged by size:
2621 1534 1369 784 748 683 433 431
414 360 314 298 245 57 40 24
NaeI GCC'GGC
Cuts at: 8536 8536
Size: 10355
NarI GG'CG_CC
Cuts at: 7786 8034 7786
Size: 248 10107
NcoI C'CATG_G
Cuts at: 107 593 2313 3395 4868 5990 5999 6578 7181
Size: 486 1720 1082 1473 1122 9 579 603
Cuts at: 7181 7263 107
Size: 82 3199
Fragments arranged by size:
3199 1720 1473 1122 1082 603 579 486
82 9
NdeI CA'TA_TG
Cuts at: 1938 6956 1938
Size: 5018 5337
NgoMIV G'CCGG_C
Cuts at: 8534 8534
Size: 10355
NheI G'CTAG_C
Cuts at: 7237 7237
Size: 10355
NsiI A_TGCA'T
Cuts at: 3872 4238 3872
Size: 366 9989
NspI r_CATG'y
Cuts at: 1150 1617 1965 2079 4236 4240 4421 5070 5613
Size: 467 348 114 2157 4 181 649 543
Cuts at: 5613 5757 6426 6801 7408 7908 9511 1150
Size: 144 669 375 607 500 1603 1994
Fragments arranged by size:
2157 1994 1603 669 649 607 543 500
467 375 348 181 144 114 4
PasI CC'CwG_GG
Cuts at: 1764 5799 6409 6429 1764
Size: 4035 610 20 5690
Fragments arranged by size:
5690 4035 610 20
PciI A'CATG_T
Cuts at: 1961 9507 1961
Size: 7546 2809
PflMI CCAn_nnn'nTGG
Cuts at: 1074 2236 5220 5331 6348 6852 7153 1074
Size: 1162 2984 111 1017 504 301 4276
Fragments arranged by size:
4276 2984 1162 1017 504 301 111
PfoI T'CCnGG_A
Cuts at: 444 630 888 3228 7152 444
Size: 186 258 2340 3924 3647
Fragments arranged by size:
3924 3647 2340 258 186
PmeI GTTT'AAAC
Cuts at: 7354 7354
Size: 10355
PmlI CAC'GTG
Cuts at: 5940 5940
Size: 10355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8801 8833 8801
Size: 32 10323
PpuMI rG'GwC_Cy
Cuts at: 544 1768 6031 6917 544
Size: 1224 4263 886 3982
Fragments arranged by size:
4263 3982 1224 886
PshAI GACnn'nnGTC
Cuts at: 4342 4342
Size: 10355
PspOMI G'GGCC_C
Cuts at: 5866 5866
Size: 10355
PstI C_TGCA'G
Cuts at: 648 2094 4344 4499 5274 5564 5880 6305 7402
Size: 1446 2250 155 775 290 316 425 1097
Cuts at: 7402 8087 648
Size: 685 2916
Fragments arranged by size:
2916 2250 1446 1097 775 685 425 316
290 155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10355
PvuII CAG'CTG
Cuts at: 348 1096 2938 5559 6345 7780 8140 348
Size: 748 1842 2621 786 1435 360 2563
Fragments arranged by size:
2621 2563 1842 1435 786 748 360
RsrII CG'GwC_CG
Cuts at: 8550 8550
Size: 10355
SacI G_AGCT'C
Cuts at: 2340 7369 2340
Size: 5029 5326
SalI G'TCGA_C
Cuts at: 7392 7392
Size: 10355
SanDI GG'GwC_CC
Cuts at: 6917 6917
Size: 10355
SapI GCTCTTCn'nnn_
Cuts at: 2591 3382 5037 8378 8588 2591
Size: 791 1655 3341 210 4358
Fragments arranged by size:
4358 3341 1655 791 210
SbfI CC_TGCA'GG
Cuts at: 7402 7402
Size: 10355
ScaI AGT'ACT
Cuts at: 3201 9872 3201
Size: 6671 3684
SfcI C'TryA_G
Cuts at: 33 257 644 2062 2090 4340 4495 5270 5560
Size: 224 387 1418 28 2250 155 775 290
Cuts at: 5560 5572 5876 6124 6301 6445 6877 7398 8083
Size: 12 304 248 177 144 432 521 685
Cuts at: 8083 9051 9242 33
Size: 968 191 1146
Fragments arranged by size:
2250 1418 1146 968 775 685 521 432
387 304 290 248 224 191 177 155
144 28 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 427 427
Size: 10355
SfoI GGC'GCC
Cuts at: 7787 8035 7787
Size: 248 10107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10355
SmaI CCC'GGG
Cuts at: 4821 7377 4821
Size: 2556 7799
SmlI C'TyrA_G
Cuts at: 832 2483 4332 5777 6654 7510 8425 9139 9401
Size: 1651 1849 1445 877 856 915 714 262
Cuts at: 9401 832
Size: 1786
Fragments arranged by size:
1849 1786 1651 1445 915 877 856 714
262
SpeI A'CTAG_T
Cuts at: 67 526 2977 67
Size: 459 2451 7445
Fragments arranged by size:
7445 2451 459
SphI G_CATG'C
Cuts at: 1150 1617 4236 5070 5613 5757 6426 7408 7908
Size: 467 2619 834 543 144 669 982 500
Cuts at: 7908 1150
Size: 3597
Fragments arranged by size:
3597 2619 982 834 669 543 500 467
144
StuI AGG'CCT
Cuts at: 1049 5234 6850 6883 1049
Size: 4185 1616 33 4521
Fragments arranged by size:
4521 4185 1616 33
StyI C'CwwG_G
Cuts at: 107 593 915 1068 1608 2313 2752 3395 3585
Size: 486 322 153 540 705 439 643 190
Cuts at: 3585 3732 4730 4868 5235 5411 5990 5999 6096
Size: 147 998 138 367 176 579 9 97
Cuts at: 6096 6139 6578 6750 6851 7093 7181 7263 7489
Size: 43 439 172 101 242 88 82 226
Cuts at: 7489 7843 107
Size: 354 2619
Fragments arranged by size:
2619 998 705 643 579 540 486 439
439 367 354 322 242 226 190 176
172 153 147 138 101 97 88 82
43 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4284 5293 6078 6591 7938 8071 8530 8739 4284
Size: 1009 785 513 1347 133 459 209 5900
Fragments arranged by size:
5900 1347 1009 785 513 459 209 133
TatI w'GTAC_w
Cuts at: 942 1324 3199 3847 5155 7333 9870 942
Size: 382 1875 648 1308 2178 2537 1427
Fragments arranged by size:
2537 2178 1875 1427 1308 648 382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3810 3842 6389 6421 7111 7143 9872 9904 3810
Size: 32 2547 32 690 32 2729 32 4261
Fragments arranged by size:
4261 2729 2547 690 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 8152 8152
Size: 10355
XbaI T'CTAG_A
Cuts at: 4011 7386 4011
Size: 3375 6980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 516 3054 5067 5997 6042 6156 7850 516
Size: 2538 2013 930 45 114 1694 3021
Fragments arranged by size:
3021 2538 2013 1694 930 114 45
XmaI C'CCGG_G
Cuts at: 4819 7375 4819
Size: 2556 7799
XmnI GAAnn'nnTTC
Cuts at: 2154 3323 3718 4163 2154
Size: 1169 395 445 8346
Fragments arranged by size:
8346 1169 445 395
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AgeI AloI AscI AvrII BmgBI BsiWI BspEI
BssHII Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI
NotI NruI PacI PsiI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI