Restriction map for
pF1KB4969
(Circular) (Six-base) MAPSORT of: pF1KB4969.seq Check: 4326 from: 1 to: 9785
pF1KB4969 9785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5603 5603
Size: 9785
AccI GT'mk_AC
Cuts at: 302 2652 3162 6823 302
Size: 2350 510 3661 3264
Fragments arranged by size:
3661 3264 2350 510
Acc65I G'GTAC_C
Cuts at: 1778 4817 6801 1778
Size: 3039 1984 4762
Fragments arranged by size:
4762 3039 1984
AclI AA'CG_TT
Cuts at: 9625 9625
Size: 9785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 2273 2624 3187 3515 3957 4092 4886 5078
Size: 1869 351 563 328 442 135 794 192
Cuts at: 5078 5867 6082 7610 8042 8789 9057 404
Size: 789 215 1528 432 747 268 1132
Fragments arranged by size:
1869 1528 1132 794 789 747 563 442
432 351 328 268 215 192 135
AflII C'TTAA_G
Cuts at: 1114 3298 1114
Size: 2184 7601
AflIII A'CryG_T
Cuts at: 6429 6666 7137 8241 9218 6429
Size: 237 471 1104 977 6996
Fragments arranged by size:
6996 1104 977 471 237
AgeI A'CCGG_T
Cuts at: 8173 8173
Size: 9785
AleI CACnn'nnGTG
Cuts at: 6578 6578
Size: 9785
AlwNI CAG_nnn'CTG
Cuts at: 933 5825 7090 8657 933
Size: 4892 1265 1567 2061
Fragments arranged by size:
4892 2061 1567 1265
ApaI G_GGCC'C
Cuts at: 674 1012 674
Size: 338 9447
ApaLI G'TGCA_C
Cuts at: 8555 8555
Size: 9785
ApoI r'AATT_y
Cuts at: 1967 2106 2140 2395 2674 3414 4304 4537 4624
Size: 139 34 255 279 740 890 233 87
Cuts at: 4624 4891 5033 5091 5410 5974 6789 9138 9394
Size: 267 142 58 319 564 815 2349 256
Cuts at: 9394 1967
Size: 2358
Fragments arranged by size:
2358 2349 890 815 740 564 319 279
267 256 255 233 142 139 87 58
34
AseI AT'TA_AT
Cuts at: 20 5531 20
Size: 5511 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9785
AvaI C'yCGr_G
Cuts at: 657 891 5006 6245 6805 657
Size: 234 4115 1239 560 3637
Fragments arranged by size:
4115 3637 1239 560 234
BamHI G'GATC_C
Cuts at: 2619 5993 6032 6810 2619
Size: 3374 39 778 5594
Fragments arranged by size:
5594 3374 778 39
BanI G'GyrC_C
Cuts at: 151 1778 4817 6046 6801 7215 7463 7498 151
Size: 1627 3039 1229 755 414 248 35 2438
Fragments arranged by size:
3039 2438 1627 1229 755 414 248 35
BanII G_rGCy'C
Cuts at: 254 674 892 1012 1383 3566 5909 6799 7829
Size: 420 218 120 371 2183 2343 890 1030
Cuts at: 7829 254
Size: 2210
Fragments arranged by size:
2343 2210 2183 1030 890 420 371 218
120
BbeI G_GCGC'C
Cuts at: 7219 7467 7219
Size: 248 9537
BbsI GAAGACnn'nnnn_
Cuts at: 727 2183 2612 3005 3300 4925 727
Size: 1456 429 393 295 1625 5587
Fragments arranged by size:
5587 1625 1456 429 393 295
BbvCI CC'TCA_GC
Cuts at: 2131 3676 3730 3811 3838 3865 3946 4081 4216
Size: 1545 54 81 27 27 81 135 135
Cuts at: 4216 4405 2131
Size: 189 7511
Fragments arranged by size:
7511 1545 189 135 135 81 81 54
27 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2379 2413 2379
Size: 34 9751
BciVI GTATCCnnnnn_n'
Cuts at: 2170 2639 7683 8444 9785 2170
Size: 469 5044 761 1341 2170
Fragments arranged by size:
5044 2170 1341 761 469
BclI T'GATC_A
Cuts at: 1520 3023 3513 4488 7305 1520
Size: 1503 490 975 2817 4000
Fragments arranged by size:
4000 2817 1503 975 490
BfrBI ATG'CAT
Cuts at: 2235 2235
Size: 9785
BglI GCCn_nnn'nGGC
Cuts at: 5284 5284
Size: 9785
BglII A'GATC_T
Cuts at: 4293 4362 6122 7300 9337 4293
Size: 69 1760 1178 2037 4741
Fragments arranged by size:
4741 2037 1760 1178 69
BlpI GC'TnA_GC
Cuts at: 2696 6897 2696
Size: 4201 5584
Bme1580I G_kGCm'C
Cuts at: 154 674 855 1012 1994 3473 4742 6411 7410
Size: 520 181 157 982 1479 1269 1669 999
Cuts at: 7410 7503 8559 154
Size: 93 1056 1380
Fragments arranged by size:
1669 1479 1380 1269 1056 999 982 520
181 157 93
BmgBI CAC'GTC
Cuts at: 1632 6430 1632
Size: 4798 4987
BmrI ACTGGGnnnn_n'
Cuts at: 1596 1997 3533 5910 6402 6997 7078 7413 9557
Size: 401 1536 2377 492 595 81 335 2144
Cuts at: 9557 1596
Size: 1824
Fragments arranged by size:
2377 2144 1824 1536 595 492 401 335
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1414 8152 1414
Size: 6738 3047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 798 955 2186 2401 2690 3434 3542 3653
Size: 277 157 1231 215 289 744 108 111
Cuts at: 3653 3680 3707 3734 3788 3815 3869 3896 3923
Size: 27 27 27 54 27 54 27 27
Cuts at: 3923 3977 4004 4058 4112 4166 4220 4328 4409
Size: 54 27 54 54 54 54 108 81
Cuts at: 4409 4463 4773 521
Size: 54 310 5533
Fragments arranged by size:
5533 1231 744 310 289 277 215 157
111 108 108 81 54 54 54 54
54 54 54 54 27 27 27 27
27 27 27
Bpu10I CC'TnA_GC
Cuts at: 199 221 248 2131 2788 3676 3730 3811 3838
Size: 22 27 1883 657 888 54 81 27
Cuts at: 3838 3865 3946 4081 4216 4405 199
Size: 27 81 135 135 189 5579
Fragments arranged by size:
5579 1883 888 657 189 135 135 81
81 54 27 27 27 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 434 456 1804 3598 6475 6961 7840 8332
Size: 188 22 1348 1794 2877 486 879 492
Cuts at: 8332 8630 246
Size: 298 1401
Fragments arranged by size:
2877 1794 1401 1348 879 492 486 298
188 22
BsaI GGTCTCn'nnnn_
Cuts at: 960 1241 9489 960
Size: 281 8248 1256
Fragments arranged by size:
8248 1256 281
BsaAI yAC'GTr
Cuts at: 213 3046 5232 5628 7138 213
Size: 2833 2186 396 1510 2860
Fragments arranged by size:
2860 2833 2186 1510 396
BsaBI GATnn'nnATC
Cuts at: 6 7299 9336 6
Size: 7293 2037 455
Fragments arranged by size:
7293 2037 455
BsaHI Gr'CG_yC
Cuts at: 7216 7464 8166 9662 7216
Size: 248 702 1496 7339
Fragments arranged by size:
7339 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2812 6974 7495 8173 8447 8594 9005 2812
Size: 4162 521 678 274 147 411 3592
Fragments arranged by size:
4162 3592 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 555 585 2042 2072 2731 2761 2790 2820 2841
Size: 30 1457 30 659 30 29 30 21
Cuts at: 2841 2871 3678 3708 3732 3762 3813 3843 4002
Size: 30 807 30 24 30 51 30 159
Cuts at: 4002 4032 4326 4356 4407 4437 4768 4798 555
Size: 30 294 30 51 30 331 30 5542
Fragments arranged by size:
5542 1457 807 659 331 294 159 51
51 30 30 30 30 30 30 30
30 30 30 30 30 29 24 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 4466 4776 4466
Size: 310 9475
BseYI C'CCAG_C
Cuts at: 158 167 879 1060 3777 4047 4155 4189 7210
Size: 9 712 181 2717 270 108 34 3021
Cuts at: 7210 7968 8545 9041 158
Size: 758 577 496 902
Fragments arranged by size:
3021 2717 902 758 712 577 496 270
181 108 34 9
BsiEI CG_ry'CG
Cuts at: 104 7327 7373 8581 104
Size: 7223 46 1208 1308
Fragments arranged by size:
7223 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 892 1870 3566 6799 7577 7767 8559 892
Size: 978 1696 3233 778 190 792 2118
Fragments arranged by size:
3233 2118 1696 978 792 778 190
BsmI GAATG_Cn'
Cuts at: 2586 3042 3303 6423 6441 2586
Size: 456 261 3120 18 5930
Fragments arranged by size:
5930 3120 456 261 18
Bsp1286I G_dGCh'C
Cuts at: 154 254 674 855 892 1012 1383 1870 1994
Size: 100 420 181 37 120 371 487 124
Cuts at: 1994 3473 3566 4742 5909 6411 6799 7410 7503
Size: 1479 93 1176 1167 502 388 611 93
Cuts at: 7503 7577 7767 7829 8559 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1479 1380 1176 1167 730 611 502 487
420 388 371 190 181 124 120 100
93 93 74 62 37
BspEI T'CCGG_A
Cuts at: 2812 9005 2812
Size: 6193 3592
BspHI T'CATG_A
Cuts at: 1640 1862 8961 1640
Size: 222 7099 2464
Fragments arranged by size:
7099 2464 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5603 6835 7049 7351 7732 5603
Size: 1232 214 302 381 7656
Fragments arranged by size:
7656 1232 381 302 214
BsrBI CCG'CTC
Cuts at: 50 8077 8131 9615 9780 50
Size: 8027 54 1484 165 55
Fragments arranged by size:
8027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1166 3361 4522 4805 4856 7697 1166
Size: 2195 1161 283 51 2841 3254
Fragments arranged by size:
3254 2841 2195 1161 283 51
BsrFI r'CCGG_y
Cuts at: 7783 7964 8173 7783
Size: 181 209 9395
Fragments arranged by size:
9395 209 181
BsrGI T'GTAC_A
Cuts at: 800 1292 4896 800
Size: 492 3604 5689
Fragments arranged by size:
5689 3604 492
BssSI C'ACGA_G
Cuts at: 1127 8056 8414 1127
Size: 6929 358 2498
Fragments arranged by size:
6929 2498 358
BstAPI GCAn_nnn'nTGC
Cuts at: 741 5977 7162 741
Size: 5236 1185 3364
Fragments arranged by size:
5236 3364 1185
BstBI TT'CG_AA
Cuts at: 4968 8146 4968
Size: 3178 6607
BstEII G'GTnAC_C
Cuts at: 3684 3684
Size: 9785
BstXI CCAn_nnnn'nTGG
Cuts at: 131 1987 2800 2851 6315 131
Size: 1856 813 51 3464 3601
Fragments arranged by size:
3601 3464 1856 813 51
BstYI r'GATC_y
Cuts at: 1 1693 2619 3547 4293 4362 4455 4794 5935
Size: 1692 926 928 746 69 93 339 1141
Cuts at: 5935 5993 6032 6122 6233 6810 7277 7300 7635
Size: 58 39 90 111 577 467 23 335
Cuts at: 7635 7881 8893 8979 8991 9337 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1692 1141 1012 928 926 746 577 467
449 346 339 335 246 111 93 90
86 69 58 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 1500 1500
Size: 9785
BtgI C'CryG_G
Cuts at: 5124 6623 5124
Size: 1499 8286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1635 7735 7916 8079 1635
Size: 6100 181 163 3341
Fragments arranged by size:
6100 3341 181 163
BtsI GCAGTG_nn'
Cuts at: 4713 6475 7028 4713
Size: 1762 553 7470
Fragments arranged by size:
7470 1762 553
DraI TTT'AAA
Cuts at: 414 5088 5715 6784 414
Size: 4674 627 1069 3415
Fragments arranged by size:
4674 3415 1069 627
DraIII CAC_nnn'GTG
Cuts at: 2665 2665
Size: 9785
DrdI GACnn_nn'nnGTC
Cuts at: 7491 8349 7491
Size: 858 8927
EaeI y'GGCC_r
Cuts at: 924 1421 3096 5643 7370 7544 7935 7962 9307
Size: 497 1675 2547 1727 174 391 27 1345
Cuts at: 9307 924
Size: 1402
Fragments arranged by size:
2547 1727 1675 1402 1345 497 391 174
27
EagI C'GGCC_G
Cuts at: 7370 7370
Size: 9785
EarI CTCTTCn'nnn_
Cuts at: 1144 1494 2041 2255 2348 3344 3558 4688 5015
Size: 350 547 214 93 996 214 1130 327
Cuts at: 5015 5440 7808 8018 1144
Size: 425 2368 210 2911
Fragments arranged by size:
2911 2368 1130 996 547 425 350 327
214 214 210 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 7993 8303 8449 9595 7993
Size: 310 146 1146 8183
Fragments arranged by size:
8183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 890 3564 6797 890
Size: 2674 3233 3878
Fragments arranged by size:
3878 3233 2674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 521 798 955 2186 2273 2401 2624 2690
Size: 117 277 157 1231 87 128 223 66
Cuts at: 2690 3187 3434 3515 3542 3653 3680 3707 3734
Size: 497 247 81 27 111 27 27 27
Cuts at: 3734 3788 3815 3869 3896 3923 3957 3977 4004
Size: 54 27 54 27 27 34 20 27
Cuts at: 4004 4058 4092 4112 4166 4220 4328 4409 4463
Size: 54 34 20 54 54 108 81 54
Cuts at: 4463 4773 4886 5078 5867 6082 7610 8042 8789
Size: 310 113 192 789 215 1528 432 747
Cuts at: 8789 9057 404
Size: 268 1132
Fragments arranged by size:
1528 1231 1132 789 747 497 432 310
277 268 247 223 215 192 157 128
117 113 111 108 87 81 81 66
54 54 54 54 54 54 34 34
27 27 27 27 27 27 27 27
20 20
EcoNI CCTnn'n_nnAGG
Cuts at: 703 1503 3679 4408 703
Size: 800 2176 729 6080
Fragments arranged by size:
6080 2176 800 729
EcoO109I rG'GnC_Cy
Cuts at: 135 671 695 930 1366 1955 2707 2822 2832
Size: 536 24 235 436 589 752 115 10
Cuts at: 2832 2873 2883 3245 5344 6924 135
Size: 41 10 362 2099 1580 2996
Fragments arranged by size:
2996 2099 1580 752 589 536 436 362
235 115 41 24 10 10
EcoRI G'AATT_C
Cuts at: 2106 2395 2674 3414 5033 6789 2106
Size: 289 279 740 1619 1756 5102
Fragments arranged by size:
5102 1756 1619 740 289 279
EcoRV GAT'ATC
Cuts at: 4469 4469
Size: 9785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7804 7804
Size: 9785
FspI TGC'GCA
Cuts at: 7566 7566
Size: 9785
HaeII r_GCGC'y
Cuts at: 7219 7467 8489 9468 7219
Size: 248 1022 979 7536
Fragments arranged by size:
7536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 429 461 640 672 1240 1272 1350 1370 1382
Size: 32 179 32 568 32 78 20 12
Cuts at: 1382 1402 1639 1671 2164 2196 3678 3710 3732
Size: 20 237 32 493 32 1482 32 22
Cuts at: 3732 3764 4002 4034 4326 4358 4407 4439 4458
Size: 32 238 32 292 32 49 32 19
Cuts at: 4458 4490 4566 4598 5314 5346 5399 5431 7627
Size: 32 76 32 716 32 53 32 2196
Cuts at: 7627 7657 7659 7689 429
Size: 30 2 30 2525
Fragments arranged by size:
2525 2196 1482 716 568 493 292 238
237 179 78 76 53 49 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 22 20
20 19 12 2
HincII GTy'rAC
Cuts at: 1485 2096 3012 6824 1485
Size: 611 916 3812 4446
Fragments arranged by size:
4446 3812 916 611
HpaI GTT'AAC
Cuts at: 2096 3012 2096
Size: 916 8869
KasI G'GCGC_C
Cuts at: 7215 7463 7215
Size: 248 9537
KpnI G_GTAC'C
Cuts at: 1782 4821 6805 1782
Size: 3039 1984 4762
Fragments arranged by size:
4762 3039 1984
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 9785
MluI A'CGCG_T
Cuts at: 9218 9218
Size: 9785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 214 369 2061 5751 6128 6265 8456 8640 214
Size: 155 1692 3690 377 137 2191 184 1359
Fragments arranged by size:
3690 2191 1692 1359 377 184 155 137
MscI TGG'CCA
Cuts at: 1423 3098 7546 1423
Size: 1675 4448 3662
Fragments arranged by size:
4448 3662 1675
MslI CAynn'nnrTG
Cuts at: 5123 5395 5626 6578 7774 7901 5123
Size: 272 231 952 1196 127 7007
Fragments arranged by size:
7007 1196 952 272 231 127
MspA1I CmG'CkG
Cuts at: 158 869 2524 3007 5305 6896 7210 7570 8583
Size: 711 1655 483 2298 1591 314 360 1013
Cuts at: 8583 8828 158
Size: 245 1115
Fragments arranged by size:
2298 1655 1591 1115 1013 711 483 360
314 245
NaeI GCC'GGC
Cuts at: 7966 7966
Size: 9785
NarI GG'CG_CC
Cuts at: 7216 7464 7216
Size: 248 9537
NcoI C'CATG_G
Cuts at: 5124 6623 5124
Size: 1499 8286
NdeI CA'TA_TG
Cuts at: 2184 4007 2184
Size: 1823 7962
NgoMIV G'CCGG_C
Cuts at: 7964 7964
Size: 9785
NsiI A_TGCA'T
Cuts at: 2237 2237
Size: 9785
NspI r_CATG'y
Cuts at: 2695 5122 5242 5394 6009 6670 6838 7338 8245
Size: 2427 120 152 615 661 168 500 907
Cuts at: 8245 2695
Size: 4235
Fragments arranged by size:
4235 2427 907 661 615 500 168 152
120
PasI CC'CwG_GG
Cuts at: 2825 6315 6423 2825
Size: 3490 108 6187
Fragments arranged by size:
6187 3490 108
PciI A'CATG_T
Cuts at: 6666 8241 6666
Size: 1575 8210
PflMI CCAn_nnn'nTGG
Cuts at: 933 4351 4378 4567 933
Size: 3418 27 189 6151
Fragments arranged by size:
6151 3418 189 27
PfoI T'CCnGG_A
Cuts at: 932 2749 4451 932
Size: 1817 1702 6266
Fragments arranged by size:
6266 1817 1702
PmeI GTTT'AAAC
Cuts at: 6784 6784
Size: 9785
PmlI CAC'GTG
Cuts at: 3046 5628 3046
Size: 2582 7203
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8924 8956 8924
Size: 32 9753
PpuMI rG'GwC_Cy
Cuts at: 135 695 930 1366 1955 2707 2832 2883 3245
Size: 560 235 436 589 752 125 51 362
Cuts at: 3245 5344 135
Size: 2099 4576
Fragments arranged by size:
4576 2099 752 589 560 436 362 235
125 51
PshAI GACnn'nnGTC
Cuts at: 3431 3539 3590 4568 3431
Size: 108 51 978 8648
Fragments arranged by size:
8648 978 108 51
PsiI TTA'TAA
Cuts at: 1280 1556 5942 6517 1280
Size: 276 4386 575 4548
Fragments arranged by size:
4548 4386 575 276
PspOMI G'GGCC_C
Cuts at: 670 1008 670
Size: 338 9447
PspXI vC'TCGA_Gb
Cuts at: 5006 6245 5006
Size: 1239 8546
PstI C_TGCA'G
Cuts at: 1176 2304 6832 7517 1176
Size: 1128 4528 685 3444
Fragments arranged by size:
4528 3444 1128 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9785
PvuII CAG'CTG
Cuts at: 158 869 2524 3007 5305 7210 7570 158
Size: 711 1655 483 2298 1905 360 2373
Fragments arranged by size:
2373 2298 1905 1655 711 483 360
RsrII CG'GwC_CG
Cuts at: 7980 7980
Size: 9785
SacI G_AGCT'C
Cuts at: 892 3566 6799 892
Size: 2674 3233 3878
Fragments arranged by size:
3878 3233 2674
SalI G'TCGA_C
Cuts at: 6822 6822
Size: 9785
SanDI GG'GwC_CC
Cuts at: 695 1955 2707 695
Size: 1260 752 7773
Fragments arranged by size:
7773 1260 752
SapI GCTCTTCn'nnn_
Cuts at: 5015 5440 7808 8018 5015
Size: 425 2368 210 6782
Fragments arranged by size:
6782 2368 425 210
SbfI CC_TGCA'GG
Cuts at: 6832 6832
Size: 9785
ScaI AGT'ACT
Cuts at: 9302 9302
Size: 9785
SfcI C'TryA_G
Cuts at: 33 303 1172 1434 1623 1944 2210 2300 2612
Size: 270 869 262 189 321 266 90 312
Cuts at: 2612 2626 5544 5826 6576 6828 7513 8506 8697
Size: 14 2918 282 750 252 685 993 191
Cuts at: 8697 33
Size: 1121
Fragments arranged by size:
2918 1121 993 869 750 685 321 312
282 270 266 262 252 191 189 90
14
SfoI GGC'GCC
Cuts at: 7217 7465 7217
Size: 248 9537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9785
SmaI CCC'GGG
Cuts at: 6807 6807
Size: 9785
SmlI C'TyrA_G
Cuts at: 261 435 449 1114 1783 3298 3577 5006 6245
Size: 174 14 665 669 1515 279 1429 1239
Cuts at: 6245 6490 6940 7855 8347 8609 261
Size: 245 450 915 492 262 1437
Fragments arranged by size:
1515 1437 1429 1239 915 669 665 492
450 279 262 245 174 14
SnaBI TAC'GTA
Cuts at: 5232 5232
Size: 9785
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9785
SphI G_CATG'C
Cuts at: 5242 6838 7338 5242
Size: 1596 500 7689
Fragments arranged by size:
7689 1596 500
SspI AAT'ATT
Cuts at: 2454 2454
Size: 9785
StyI C'CwwG_G
Cuts at: 177 2711 4350 4566 5124 5989 6042 6449 6623
Size: 2534 1639 216 558 865 53 407 174
Cuts at: 6623 6919 7273 177
Size: 296 354 2689
Fragments arranged by size:
2689 2534 1639 865 558 407 354 296
216 174 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 3116 3602 3775 3910 4045 4153 5571 7368
Size: 2512 486 173 135 135 108 1418 1797
Cuts at: 7368 7501 7960 8169 604
Size: 133 459 209 2220
Fragments arranged by size:
2512 2220 1797 1418 486 459 209 173
135 135 133 108
TatI w'GTAC_w
Cuts at: 800 1292 4896 9300 800
Size: 492 3604 4404 1285
Fragments arranged by size:
4404 3604 1285 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 555 587 2042 2074 2731 2763 2790 2822 2841
Size: 32 1455 32 657 32 27 32 19
Cuts at: 2841 2873 9302 9334 555
Size: 32 6429 32 1006
Fragments arranged by size:
6429 1455 1006 657 32 32 32 32
32 32 27 19
Tth111I GACn'n_nGTC
Cuts at: 1927 7582 1927
Size: 5655 4130
XbaI T'CTAG_A
Cuts at: 3966 4101 4209 6816 3966
Size: 135 108 2607 6935
Fragments arranged by size:
6935 2607 135 108
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1019 7280 1019
Size: 6261 3524
XhoI C'TCGA_G
Cuts at: 5006 6245 5006
Size: 1239 8546
XmaI C'CCGG_G
Cuts at: 6805 6805
Size: 9785
XmnI GAAnn'nnTTC
Cuts at: 3758 3771 6679 3758
Size: 13 2908 6864
Fragments arranged by size:
6864 2908 13
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI AscI AvrII BaeI
BmtI BsgI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
FseI FspAI HindIII NheI NotI NruI PacI PsrI
SacII SexAI SfiI SgrAI SrfI StuI SwaI ZraI