Restriction map for
pF1KB4980
(Circular) (Six-base) MAPSORT of: pF1KB4980.seq Check: 3366 from: 1 to: 5654
pF1KB4980 5654 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 191 2282 191
Size: 2091 3563
AccI GT'mk_AC
Cuts at: 167 2692 167
Size: 2525 3129
Acc65I G'GTAC_C
Cuts at: 2670 2670
Size: 5654
AclI AA'CG_TT
Cuts at: 5494 5494
Size: 5654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 406 721 976 1498 1779 3479 3911 4264
Size: 123 315 255 522 281 1700 432 353
Cuts at: 4264 4926 283
Size: 662 1011
Fragments arranged by size:
1700 1011 662 522 432 353 315 281
255 123
AflIII A'CryG_T
Cuts at: 2246 3006 4806 5087 2246
Size: 760 1800 281 2813
Fragments arranged by size:
2813 1800 760 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 355 355
Size: 5654
AlwNI CAG_nnn'CTG
Cuts at: 286 735 954 1323 2337 2959 4397 286
Size: 449 219 369 1014 622 1438 1543
Fragments arranged by size:
1543 1438 1014 622 449 369 219
ApaI G_GGCC'C
Cuts at: 1020 1020
Size: 5654
ApaLI G'TGCA_C
Cuts at: 4492 4492
Size: 5654
ApoI r'AATT_y
Cuts at: 2658 5007 5263 2658
Size: 2349 256 3049
Fragments arranged by size:
3049 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5654
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5654
AvaI C'yCGr_G
Cuts at: 866 2674 866
Size: 1808 3846
BamHI G'GATC_C
Cuts at: 707 2679 707
Size: 1972 3682
BanI G'GyrC_C
Cuts at: 1443 2106 2172 2670 3084 3332 3367 1443
Size: 663 66 498 414 248 35 3730
Fragments arranged by size:
3730 663 498 414 248 66 35
BanII G_rGCy'C
Cuts at: 860 1020 1772 2090 2668 3698 860
Size: 160 752 318 578 1030 2816
Fragments arranged by size:
2816 1030 752 578 318 160
BbeI G_GCGC'C
Cuts at: 3088 3336 3088
Size: 248 5406
BbsI GAAGACnn'nnnn_
Cuts at: 964 2420 2477 964
Size: 1456 57 4141
Fragments arranged by size:
4141 1456 57
BbvCI CC'TCA_GC
Cuts at: 572 1490 572
Size: 918 4736
BciVI GTATCCnnnnn_n'
Cuts at: 3552 4608 5654 3552
Size: 1056 1046 3552
Fragments arranged by size:
3552 1056 1046
BclI T'GATC_A
Cuts at: 1718 3174 1718
Size: 1456 4198
BglI GCCn_nnn'nGGC
Cuts at: 1850 1850
Size: 5654
BglII A'GATC_T
Cuts at: 1547 3169 5206 1547
Size: 1622 2037 1995
Fragments arranged by size:
2037 1995 1622
BlpI GC'TnA_GC
Cuts at: 1436 1937 2766 1436
Size: 501 829 4324
Fragments arranged by size:
4324 829 501
Bme1580I G_kGCm'C
Cuts at: 273 1020 1145 1234 1424 1448 2177 2458 2568
Size: 747 125 89 190 24 729 281 110
Cuts at: 2568 3279 3372 4496 273
Size: 711 93 1124 1431
Fragments arranged by size:
1431 1124 747 729 711 281 190 125
110 93 89 24
BmrI ACTGGGnnnn_n'
Cuts at: 635 1921 2636 2866 2947 3282 5426 635
Size: 1286 715 230 81 335 2144 863
Fragments arranged by size:
2144 1286 863 715 335 230 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1103 4021 1103
Size: 2918 2736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 689 763 1001 1124 1139 1300 2074 2548
Size: 460 74 238 123 15 161 774 474
Cuts at: 2548 229
Size: 3335
Fragments arranged by size:
3335 774 474 460 238 161 123 74
15
Bpu10I CC'TnA_GC
Cuts at: 572 1490 2425 572
Size: 918 935 3801
Fragments arranged by size:
3801 935 918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 475 874 1023 1186 2830 3709 4423 4721
Size: 96 399 149 163 1644 879 714 298
Cuts at: 4721 379
Size: 1312
Fragments arranged by size:
1644 1312 879 714 399 298 163 149
96
BsaI GGTCTCn'nnnn_
Cuts at: 132 655 5358 132
Size: 523 4703 428
Fragments arranged by size:
4703 523 428
BsaAI yAC'GTr
Cuts at: 3007 3007
Size: 5654
BsaBI GATnn'nnATC
Cuts at: 6 3168 5205 6
Size: 3162 2037 455
Fragments arranged by size:
3162 2037 455
BsaHI Gr'CG_yC
Cuts at: 245 3085 3333 4035 5531 245
Size: 2840 248 702 1496 368
Fragments arranged by size:
2840 1496 702 368 248
BsaWI w'CCGG_w
Cuts at: 1694 1982 2843 3364 4042 4453 4600 4874 1694
Size: 288 861 521 678 411 147 274 2474
Fragments arranged by size:
2474 861 678 521 411 288 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1444 1474 1676 1706 2185 2215 2558 2588 1444
Size: 30 202 30 479 30 343 30 4510
Fragments arranged by size:
4510 479 343 202 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 775 1264 1777 2474 775
Size: 489 513 697 3955
Fragments arranged by size:
3955 697 513 489
BseYI C'CCAG_C
Cuts at: 815 1020 1629 2034 2287 3079 3837 4502 4910
Size: 205 609 405 253 792 758 665 408
Cuts at: 4910 815
Size: 1559
Fragments arranged by size:
1559 792 758 665 609 408 405 253
205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 577 172
Size: 405 5249
BsiEI CG_ry'CG
Cuts at: 104 3196 3242 4472 104
Size: 3092 46 1230 1286
Fragments arranged by size:
3092 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 334 1268 1526 2090 2668 3446 3636 4496 334
Size: 934 258 564 578 778 190 860 1492
Fragments arranged by size:
1492 934 860 778 578 564 258 190
BsmI GAATG_Cn'
Cuts at: 1157 1157
Size: 5654
Bsp1286I G_dGCh'C
Cuts at: 273 334 860 1020 1145 1234 1268 1424 1448
Size: 61 526 160 125 89 34 156 24
Cuts at: 1448 1526 1772 2090 2177 2458 2568 2668 3279
Size: 78 246 318 87 281 110 100 611
Cuts at: 3279 3372 3446 3636 3698 4496 273
Size: 93 74 190 62 798 1431
Fragments arranged by size:
1431 798 611 526 318 281 246 190
160 156 125 110 100 93 89 87
78 74 62 61 34 24
BspEI T'CCGG_A
Cuts at: 1694 1982 1694
Size: 288 5366
BspHI T'CATG_A
Cuts at: 4086 4086
Size: 5654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 1202 2282 2704 2918 3220 3601 191
Size: 1011 1080 422 214 302 381 2244
Fragments arranged by size:
2244 1080 1011 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1224 1440 3946 4000 5484 5649 50
Size: 1174 216 2506 54 1484 165 55
Fragments arranged by size:
2506 1484 1174 216 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1823 3566 1823
Size: 1743 3911
BsrFI r'CCGG_y
Cuts at: 884 1712 3652 3833 884
Size: 828 1940 181 2705
Fragments arranged by size:
2705 1940 828 181
BssSI C'ACGA_G
Cuts at: 3925 4633 3925
Size: 708 4946
BstAPI GCAn_nnn'nTGC
Cuts at: 3031 3031
Size: 5654
BstBI TT'CG_AA
Cuts at: 4015 4015
Size: 5654
BstYI r'GATC_y
Cuts at: 1 536 707 1547 1622 1929 2606 2679 3146
Size: 535 171 840 75 307 677 73 467
Cuts at: 3146 3169 3504 3750 4056 4068 4154 5206 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 840 677 535 467 449 335 307
306 246 171 86 75 73 23 12
Bsu36I CC'TnA_GG
Cuts at: 1088 2354 2450 2629 1088
Size: 1266 96 179 4113
Fragments arranged by size:
4113 1266 179 96
BtgI C'CryG_G
Cuts at: 1350 1635 2054 2102 2405 1350
Size: 285 419 48 303 4599
Fragments arranged by size:
4599 419 303 285 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 857 3604 3785 3948 857
Size: 2747 181 163 2563
Fragments arranged by size:
2747 2563 181 163
BtsI GCAGTG_nn'
Cuts at: 1660 2897 1660
Size: 1237 4417
DraI TTT'AAA
Cuts at: 2653 2653
Size: 5654
DrdI GACnn_nn'nnGTC
Cuts at: 3360 4704 3360
Size: 1344 4310
EaeI y'GGCC_r
Cuts at: 395 579 644 882 3239 3413 3804 3831 5176
Size: 184 65 238 2357 174 391 27 1345
Cuts at: 5176 395
Size: 873
Fragments arranged by size:
2357 1345 873 391 238 184 174 65
27
EagI C'GGCC_G
Cuts at: 3239 3239
Size: 5654
EarI CTCTTCn'nnn_
Cuts at: 697 1273 1537 3677 3887 697
Size: 576 264 2140 210 2464
Fragments arranged by size:
2464 2140 576 264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 439 3862 4604 4750 5464 439
Size: 3423 742 146 714 629
Fragments arranged by size:
3423 742 714 629 146
EcoICRI GAG'CTC
Cuts at: 2088 2666 2088
Size: 578 5076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 283 406 689 721 763 976 1001 1124
Size: 54 123 283 32 42 213 25 123
Cuts at: 1124 1139 1300 1498 1779 2074 2548 3479 3911
Size: 15 161 198 281 295 474 931 432
Cuts at: 3911 4264 4926 229
Size: 353 662 957
Fragments arranged by size:
957 931 662 474 432 353 295 283
281 213 198 161 123 123 54 42
32 25 15
EcoNI CCTnn'n_nnAGG
Cuts at: 562 562
Size: 5654
EcoO109I rG'GnC_Cy
Cuts at: 378 639 1016 1017 1093 1320 2334 2508 2555
Size: 261 377 1 76 227 1014 174 47
Cuts at: 2555 2793 378
Size: 238 3239
Fragments arranged by size:
3239 1014 377 261 238 227 174 76
47 1
EcoRI G'AATT_C
Cuts at: 2658 2658
Size: 5654
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 522 3673 522
Size: 3151 2503
FspI TGC'GCA
Cuts at: 3435 3435
Size: 5654
HaeII r_GCGC'y
Cuts at: 3088 3336 4566 5337 3088
Size: 248 1230 771 3405
Fragments arranged by size:
3405 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 354 386 594 626 1112 1144 3496 3526 3528
Size: 32 208 32 486 32 2352 30 2
Cuts at: 3528 3558 354
Size: 30 2450
Fragments arranged by size:
2450 2352 486 208 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 168 2244 2693 168
Size: 2076 449 3129
Fragments arranged by size:
3129 2076 449
HindIII A'AGCT_T
Cuts at: 2518 2518
Size: 5654
KasI G'GCGC_C
Cuts at: 3084 3332 3084
Size: 248 5406
KpnI G_GTAC'C
Cuts at: 2674 2674
Size: 5654
MluI A'CGCG_T
Cuts at: 5087 5087
Size: 5654
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 4413 4597 266
Size: 4147 184 1323
Fragments arranged by size:
4147 1323 184
MscI TGG'CCA
Cuts at: 397 581 646 3415 397
Size: 184 65 2769 2636
Fragments arranged by size:
2769 2636 184 65
MslI CAynn'nnrTG
Cuts at: 2047 2149 3643 3770 2047
Size: 102 1494 127 3931
Fragments arranged by size:
3931 1494 127 102
MspA1I CmG'CkG
Cuts at: 156 735 1035 2420 2765 3079 3439 4223 4468
Size: 579 300 1385 345 314 360 784 245
Cuts at: 4468 156
Size: 1342
Fragments arranged by size:
1385 1342 784 579 360 345 314 300
245
NaeI GCC'GGC
Cuts at: 886 3835 886
Size: 2949 2705
NarI GG'CG_CC
Cuts at: 3085 3333 3085
Size: 248 5406
NcoI C'CATG_G
Cuts at: 1350 2102 2405 1350
Size: 752 303 4599
Fragments arranged by size:
4599 752 303
NgoMIV G'CCGG_C
Cuts at: 884 3833 884
Size: 2949 2705
NspI r_CATG'y
Cuts at: 2154 2365 2707 3207 4810 2154
Size: 211 342 500 1603 2998
Fragments arranged by size:
2998 1603 500 342 211
PasI CC'CwG_GG
Cuts at: 221 1448 2331 221
Size: 1227 883 3544
Fragments arranged by size:
3544 1227 883
PciI A'CATG_T
Cuts at: 4806 4806
Size: 5654
PfoI T'CCnGG_A
Cuts at: 779 1670 1925 2261 2525 779
Size: 891 255 336 264 3908
Fragments arranged by size:
3908 891 336 264 255
PmeI GTTT'AAAC
Cuts at: 2653 2653
Size: 5654
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1159 1191 2495 2527 4100 4132 1159
Size: 32 1304 32 1573 32 2681
Fragments arranged by size:
2681 1573 1304 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 378 1093 2508 2555 378
Size: 715 1415 47 3477
Fragments arranged by size:
3477 1415 715 47
PspOMI G'GGCC_C
Cuts at: 1016 1016
Size: 5654
PstI C_TGCA'G
Cuts at: 521 563 659 2279 2582 2701 3386 521
Size: 42 96 1620 303 119 685 2789
Fragments arranged by size:
2789 1620 685 303 119 96 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5654
PvuII CAG'CTG
Cuts at: 735 2420 3079 3439 735
Size: 1685 659 360 2950
Fragments arranged by size:
2950 1685 659 360
RsrII CG'GwC_CG
Cuts at: 3849 3849
Size: 5654
SacI G_AGCT'C
Cuts at: 2090 2668 2090
Size: 578 5076
SalI G'TCGA_C
Cuts at: 166 2691 166
Size: 2525 3129
SanDI GG'GwC_CC
Cuts at: 378 378
Size: 5654
SapI GCTCTTCn'nnn_
Cuts at: 1273 3677 3887 1273
Size: 2404 210 3040
Fragments arranged by size:
3040 2404 210
SbfI CC_TGCA'GG
Cuts at: 2701 2701
Size: 5654
ScaI AGT'ACT
Cuts at: 5171 5171
Size: 5654
SfcI C'TryA_G
Cuts at: 33 517 559 655 820 2275 2578 2697 3382
Size: 484 42 96 165 1455 303 119 685
Cuts at: 3382 4350 4541 33
Size: 968 191 1146
Fragments arranged by size:
1455 1146 968 685 484 303 191 165
119 96 42
SfoI GGC'GCC
Cuts at: 3086 3334 3086
Size: 248 5406
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5654
SmaI CCC'GGG
Cuts at: 868 2676 868
Size: 1808 3846
SmlI C'TyrA_G
Cuts at: 358 490 853 1002 1201 2809 3724 4438 4700
Size: 132 363 149 199 1608 915 714 262
Cuts at: 4700 358
Size: 1312
Fragments arranged by size:
1608 1312 915 714 363 262 199 149
132
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5654
SphI G_CATG'C
Cuts at: 2154 2365 2707 3207 2154
Size: 211 342 500 4601
Fragments arranged by size:
4601 500 342 211
StuI AGG'CCT
Cuts at: 304 1579 2222 304
Size: 1275 643 3736
Fragments arranged by size:
3736 1275 643
StyI C'CwwG_G
Cuts at: 1145 1350 1967 2102 2121 2405 2558 2788 3142
Size: 205 617 135 19 284 153 230 354
Cuts at: 3142 1145
Size: 3657
Fragments arranged by size:
3657 617 354 284 230 205 153 135
19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 1934 3237 3370 3829 4038 187
Size: 1747 1303 133 459 209 1803
Fragments arranged by size:
1803 1747 1303 459 209 133
TatI w'GTAC_w
Cuts at: 5169 5169
Size: 5654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1444 1476 2185 2217 2558 2590 5171 5203 1444
Size: 32 709 32 341 32 2581 32 1895
Fragments arranged by size:
2581 1895 709 341 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 286 3451 286
Size: 3165 2489
XbaI T'CTAG_A
Cuts at: 2685 2685
Size: 5654
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 924 1032 1908 3149 924
Size: 108 876 1241 3429
Fragments arranged by size:
3429 1241 876 108
XmaI C'CCGG_G
Cuts at: 866 2674 866
Size: 1808 3846
XmnI GAAnn'nnTTC
Cuts at: 1549 1549
Size: 5654
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI