Restriction map for
pF1KB4982
(Circular) (Six-base) MAPSORT of: pF1KB4982.seq Check: 4172 from: 1 to: 5516
pF1KB4982 5516 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 2554 2554
Size: 5516
Acc65I G'GTAC_C
Cuts at: 2532 2532
Size: 5516
AclI AA'CG_TT
Cuts at: 695 5356 695
Size: 4661 855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 1130 3341 3773 4126 4788 524
Size: 606 2211 432 353 662 1252
Fragments arranged by size:
2211 1252 662 606 432 353
AflII C'TTAA_G
Cuts at: 2128 2128
Size: 5516
AflIII A'CryG_T
Cuts at: 2868 4668 4949 2868
Size: 1800 281 3435
Fragments arranged by size:
3435 1800 281
AleI CACnn'nnGTG
Cuts at: 1514 1964 1514
Size: 450 5066
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 5516
AlwNI CAG_nnn'CTG
Cuts at: 850 2440 2821 4259 850
Size: 1590 381 1438 2107
Fragments arranged by size:
2107 1590 1438 381
ApaI G_GGCC'C
Cuts at: 2372 2372
Size: 5516
ApaLI G'TGCA_C
Cuts at: 4354 4354
Size: 5516
ApoI r'AATT_y
Cuts at: 1525 1605 2520 4869 5125 1525
Size: 80 915 2349 256 1916
Fragments arranged by size:
2349 1916 915 256 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 5516
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5516
AvaI C'yCGr_G
Cuts at: 2536 2536
Size: 5516
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2034 2067 2034
Size: 33 5483
BamHI G'GATC_C
Cuts at: 2541 2541
Size: 5516
BanI G'GyrC_C
Cuts at: 628 2532 2946 3194 3229 628
Size: 1904 414 248 35 2915
Fragments arranged by size:
2915 1904 414 248 35
BanII G_rGCy'C
Cuts at: 295 899 2372 2530 3560 295
Size: 604 1473 158 1030 2251
Fragments arranged by size:
2251 1473 1030 604 158
BbeI G_GCGC'C
Cuts at: 2950 3198 2950
Size: 248 5268
BbsI GAAGACnn'nnnn_
Cuts at: 631 1193 2459 631
Size: 562 1266 3688
Fragments arranged by size:
3688 1266 562
BbvCI CC'TCA_GC
Cuts at: 1072 1072
Size: 5516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5482
BciVI GTATCCnnnnn_n'
Cuts at: 3414 4470 5516 3414
Size: 1056 1046 3414
Fragments arranged by size:
3414 1056 1046
BclI T'GATC_A
Cuts at: 3036 3036
Size: 5516
BfrBI ATG'CAT
Cuts at: 111 879 111
Size: 768 4748
BglII A'GATC_T
Cuts at: 2427 3031 5068 2427
Size: 604 2037 2875
Fragments arranged by size:
2875 2037 604
BlpI GC'TnA_GC
Cuts at: 2628 2628
Size: 5516
Bme1580I G_kGCm'C
Cuts at: 633 960 1894 2372 3141 3234 4358 633
Size: 327 934 478 769 93 1124 1791
Fragments arranged by size:
1791 1124 934 769 478 327 93
BmrI ACTGGGnnnn_n'
Cuts at: 253 641 2123 2728 2809 3144 5288 253
Size: 388 1482 605 81 335 2144 481
Fragments arranged by size:
2144 1482 605 481 388 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2430 3883 2430
Size: 1453 4063
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 777 913 1415 2110 2361 427
Size: 350 136 502 695 251 3582
Fragments arranged by size:
3582 695 502 350 251 136
Bpu10I CC'TnA_GC
Cuts at: 289 1072 289
Size: 783 4733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 2158 2183 2262 2692 3571 4285 4583 2002
Size: 156 25 79 430 879 714 298 2935
Fragments arranged by size:
2935 879 714 430 298 156 79 25
BsaI GGTCTCn'nnnn_
Cuts at: 5220 5220
Size: 5516
BsaAI yAC'GTr
Cuts at: 2184 2869 2184
Size: 685 4831
BsaBI GATnn'nnATC
Cuts at: 6 554 1739 3030 5067 6
Size: 548 1185 1291 2037 455
Fragments arranged by size:
2037 1291 1185 548 455
BsaHI Gr'CG_yC
Cuts at: 2947 3195 3897 5393 2947
Size: 248 702 1496 3070
Fragments arranged by size:
3070 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2705 3226 3904 4315 4462 4736 2705
Size: 521 678 411 147 274 3485
Fragments arranged by size:
3485 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 369 399 1021 1051 1364 1394 369
Size: 30 622 30 313 30 4491
Fragments arranged by size:
4491 622 313 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 1086 2410 754
Size: 332 1324 3860
Fragments arranged by size:
3860 1324 332
BseYI C'CCAG_C
Cuts at: 1996 2941 3699 4364 4772 1996
Size: 945 758 665 408 2740
Fragments arranged by size:
2740 945 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1629 2486 1629
Size: 857 4659
BsiEI CG_ry'CG
Cuts at: 104 3058 3104 4334 104
Size: 2954 46 1230 1286
Fragments arranged by size:
2954 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 295 899 994 1885 2189 2530 3308 3498 4358
Size: 604 95 891 304 341 778 190 860
Cuts at: 4358 295
Size: 1453
Fragments arranged by size:
1453 891 860 778 604 341 304 190
95
BsmI GAATG_Cn'
Cuts at: 771 771
Size: 5516
Bsp1286I G_dGCh'C
Cuts at: 295 633 899 960 994 1885 1894 2189 2372
Size: 338 266 61 34 891 9 295 183
Cuts at: 2372 2530 3141 3234 3308 3498 3560 4358 295
Size: 158 611 93 74 190 62 798 1453
Fragments arranged by size:
1453 891 798 611 338 295 266 190
183 158 93 74 62 61 34 9
BspHI T'CATG_A
Cuts at: 801 1623 2277 3948 801
Size: 822 654 1671 2369
Fragments arranged by size:
2369 1671 822 654
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2012 2368 2566 2780 3082 3463 2012
Size: 356 198 214 302 381 4065
Fragments arranged by size:
4065 381 356 302 214 198
BsrBI CCG'CTC
Cuts at: 50 3808 3862 5346 5511 50
Size: 3758 54 1484 165 55
Fragments arranged by size:
3758 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 186 923 3428 186
Size: 737 2505 2274
Fragments arranged by size:
2505 2274 737
BsrFI r'CCGG_y
Cuts at: 3514 3695 3514
Size: 181 5335
BsrGI T'GTAC_A
Cuts at: 2311 2311
Size: 5516
BssSI C'ACGA_G
Cuts at: 3787 4495 3787
Size: 708 4808
BstAPI GCAn_nnn'nTGC
Cuts at: 1763 2184 2893 1763
Size: 421 709 4386
Fragments arranged by size:
4386 709 421
BstBI TT'CG_AA
Cuts at: 3877 3877
Size: 5516
BstEII G'GTnAC_C
Cuts at: 359 705 359
Size: 346 5170
BstXI CCAn_nnnn'nTGG
Cuts at: 1066 1066
Size: 5516
BstYI r'GATC_y
Cuts at: 1 549 2427 2541 3008 3031 3366 3612 3918
Size: 548 1878 114 467 23 335 246 306
Cuts at: 3918 3930 4016 5068 1
Size: 12 86 1052 449
Fragments arranged by size:
1878 1052 548 467 449 335 306 246
114 86 23 12
BtgI C'CryG_G
Cuts at: 1454 1845 1454
Size: 391 5125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3466 3647 3810 3466
Size: 181 163 5172
Fragments arranged by size:
5172 181 163
BtsI GCAGTG_nn'
Cuts at: 1174 2759 1174
Size: 1585 3931
DraI TTT'AAA
Cuts at: 2515 2515
Size: 5516
DraIII CAC_nnn'GTG
Cuts at: 628 981 628
Size: 353 5163
DrdI GACnn_nn'nnGTC
Cuts at: 3222 4566 3222
Size: 1344 4172
EaeI y'GGCC_r
Cuts at: 654 983 1437 3101 3275 3666 3693 5038 654
Size: 329 454 1664 174 391 27 1345 1132
Fragments arranged by size:
1664 1345 1132 454 391 329 174 27
EagI C'GGCC_G
Cuts at: 3101 3101
Size: 5516
EarI CTCTTCn'nnn_
Cuts at: 1592 2399 2414 3539 3749 1592
Size: 807 15 1125 210 3359
Fragments arranged by size:
3359 1125 807 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3724 4466 4612 5326 3724
Size: 742 146 714 3914
Fragments arranged by size:
3914 742 714 146
EcoICRI GAG'CTC
Cuts at: 293 897 2528 293
Size: 604 1631 3281
Fragments arranged by size:
3281 1631 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 524 777 913 1130 1415 2110 2361 3341
Size: 97 253 136 217 285 695 251 980
Cuts at: 3341 3773 4126 4788 427
Size: 432 353 662 1155
Fragments arranged by size:
1155 980 695 662 432 353 285 253
251 217 136 97
EcoNI CCTnn'n_nnAGG
Cuts at: 248 248
Size: 5516
EcoO109I rG'GnC_Cy
Cuts at: 280 2169 2368 2655 280
Size: 1889 199 287 3141
Fragments arranged by size:
3141 1889 287 199
EcoRI G'AATT_C
Cuts at: 1525 2520 1525
Size: 995 4521
EcoRV GAT'ATC
Cuts at: 1659 1659
Size: 5516
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2256 3535 2256
Size: 1279 4237
FspI TGC'GCA
Cuts at: 1579 3297 1579
Size: 1718 3798
HaeII r_GCGC'y
Cuts at: 2950 3198 4428 5199 2950
Size: 248 1230 771 3267
Fragments arranged by size:
3267 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 595 627 2202 2234 2235 2267 3358 3388 3390
Size: 32 1575 32 1 32 1091 30 2
Cuts at: 3390 3420 595
Size: 30 2691
Fragments arranged by size:
2691 1575 1091 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1488 2555 1488
Size: 1067 4449
HindIII A'AGCT_T
Cuts at: 1898 1898
Size: 5516
HpaI GTT'AAC
Cuts at: 1488 1488
Size: 5516
KasI G'GCGC_C
Cuts at: 2946 3194 2946
Size: 248 5268
KpnI G_GTAC'C
Cuts at: 2536 2536
Size: 5516
MfeI C'AATT_G
Cuts at: 1364 1364
Size: 5516
MluI A'CGCG_T
Cuts at: 4949 4949
Size: 5516
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 555 4275 4459 555
Size: 3720 184 1612
Fragments arranged by size:
3720 1612 184
MscI TGG'CCA
Cuts at: 656 985 1439 3277 656
Size: 329 454 1838 2895
Fragments arranged by size:
2895 1838 454 329
MslI CAynn'nnrTG
Cuts at: 647 863 884 1136 1408 1514 1733 1948 1964
Size: 216 21 252 272 106 219 215 16
Cuts at: 1964 3505 3632 647
Size: 1541 127 2531
Fragments arranged by size:
2531 1541 272 252 219 216 215 127
106 21 16
MspA1I CmG'CkG
Cuts at: 159 2485 2627 2941 3301 4085 4330 159
Size: 2326 142 314 360 784 245 1345
Fragments arranged by size:
2326 1345 784 360 314 245 142
NaeI GCC'GGC
Cuts at: 3697 3697
Size: 5516
NarI GG'CG_CC
Cuts at: 2947 3195 2947
Size: 248 5268
NcoI C'CATG_G
Cuts at: 1454 1845 1454
Size: 391 5125
NgoMIV G'CCGG_C
Cuts at: 3695 3695
Size: 5516
NsiI A_TGCA'T
Cuts at: 113 881 113
Size: 768 4748
NspI r_CATG'y
Cuts at: 948 2569 3069 4672 948
Size: 1621 500 1603 1792
Fragments arranged by size:
1792 1621 1603 500
PasI CC'CwG_GG
Cuts at: 1827 1827
Size: 5516
PciI A'CATG_T
Cuts at: 4668 4668
Size: 5516
PflMI CCAn_nnn'nTGG
Cuts at: 663 1935 663
Size: 1272 4244
PmeI GTTT'AAAC
Cuts at: 2515 2515
Size: 5516
PmlI CAC'GTG
Cuts at: 2184 2184
Size: 5516
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 3962
Size: 32 5484
PpuMI rG'GwC_Cy
Cuts at: 280 2169 280
Size: 1889 3627
PspOMI G'GGCC_C
Cuts at: 2368 2368
Size: 5516
PstI C_TGCA'G
Cuts at: 2563 3248 2563
Size: 685 4831
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5516
PvuII CAG'CTG
Cuts at: 159 2941 3301 159
Size: 2782 360 2374
Fragments arranged by size:
2782 2374 360
RsrII CG'GwC_CG
Cuts at: 3711 3711
Size: 5516
SacI G_AGCT'C
Cuts at: 295 899 2530 295
Size: 604 1631 3281
Fragments arranged by size:
3281 1631 604
SalI G'TCGA_C
Cuts at: 2553 2553
Size: 5516
SanDI GG'GwC_CC
Cuts at: 280 280
Size: 5516
SapI GCTCTTCn'nnn_
Cuts at: 1592 3539 3749 1592
Size: 1947 210 3359
Fragments arranged by size:
3359 1947 210
SbfI CC_TGCA'GG
Cuts at: 2563 2563
Size: 5516
ScaI AGT'ACT
Cuts at: 5033 5033
Size: 5516
SfcI C'TryA_G
Cuts at: 33 851 1974 2043 2457 2559 3244 4212 4403
Size: 818 1123 69 414 102 685 968 191
Cuts at: 4403 33
Size: 1146
Fragments arranged by size:
1146 1123 968 818 685 414 191 102
69
SfoI GGC'GCC
Cuts at: 2948 3196 2948
Size: 248 5268
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5516
SmaI CCC'GGG
Cuts at: 2538 2538
Size: 5516
SmlI C'TyrA_G
Cuts at: 2017 2128 2173 2198 2241 2671 3586 4300 4562
Size: 111 45 25 43 430 915 714 262
Cuts at: 4562 2017
Size: 2971
Fragments arranged by size:
2971 915 714 430 262 111 45 43
25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5516
SphI G_CATG'C
Cuts at: 2569 3069 2569
Size: 500 5016
StuI AGG'CCT
Cuts at: 254 254
Size: 5516
StyI C'CwwG_G
Cuts at: 668 1454 1845 2650 3004 668
Size: 786 391 805 354 3180
Fragments arranged by size:
3180 805 786 391 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 1120 1745 2089 3099 3232 3691 3900 240
Size: 880 625 344 1010 133 459 209 1856
Fragments arranged by size:
1856 1010 880 625 459 344 209 133
TatI w'GTAC_w
Cuts at: 476 1237 2311 5031 476
Size: 761 1074 2720 961
Fragments arranged by size:
2720 1074 961 761
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 369 401 464 496 1643 1675 2077 2109 2454
Size: 32 63 32 1147 32 402 32 345
Cuts at: 2454 2486 5033 5065 369
Size: 32 2547 32 820
Fragments arranged by size:
2547 1147 820 402 345 63 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3313 3313
Size: 5516
XbaI T'CTAG_A
Cuts at: 2547 2547
Size: 5516
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 784 978 3011 784
Size: 194 2033 3289
Fragments arranged by size:
3289 2033 194
XmaI C'CCGG_G
Cuts at: 2536 2536
Size: 5516
XmnI GAAnn'nnTTC
Cuts at: 1403 1403
Size: 5516
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI AscI AvrII
BglI BmgBI BmtI BsiWI BsmBI BspEI BssHII BstZ17I
Bsu36I ClaI CspCI FseI FspAI NdeI NheI NotI
NruI PacI PfoI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI