Restriction map for
pF1KB4983
(Circular) (Six-base) MAPSORT of: pF1KB4983.seq Check: 6487 from: 1 to: 4640
pF1KB4983 4640 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 1678 1678
Size: 4640
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1319 2465 2897 3644 3912 1031
Size: 288 1146 432 747 268 1759
Fragments arranged by size:
1759 1146 747 432 288 268
AflIII A'CryG_T
Cuts at: 1992 3096 4073 1992
Size: 1104 977 2559
Fragments arranged by size:
2559 1104 977
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 1486 1945 3512 1486
Size: 459 1567 2614
Fragments arranged by size:
2614 1567 459
ApaLI G'TGCA_C
Cuts at: 1252 3410 1252
Size: 2158 2482
ApoI r'AATT_y
Cuts at: 462 835 919 1068 1115 1145 1366 1644 3993
Size: 373 84 149 47 30 221 278 2349
Cuts at: 3993 4249 462
Size: 256 853
Fragments arranged by size:
2349 853 373 278 256 221 149 84
47 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4640
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 1660 1660
Size: 4640
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 692 725 692
Size: 33 4607
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 1656 2070 2318 2353 1656
Size: 414 248 35 3943
Fragments arranged by size:
3943 414 248 35
BanII G_rGCy'C
Cuts at: 1654 2684 1654
Size: 1030 3610
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4606
BciVI GTATCCnnnnn_n'
Cuts at: 2538 3299 4640 2538
Size: 761 1341 2538
Fragments arranged by size:
2538 1341 761
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglII A'GATC_T
Cuts at: 2155 4192 2155
Size: 2037 2603
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 1256 2265 2358 3414 1256
Size: 1009 93 1056 2482
Fragments arranged by size:
2482 1056 1009 93
BmrI ACTGGGnnnn_n'
Cuts at: 285 1468 1852 1933 2268 4412 285
Size: 1183 384 81 335 2144 513
Fragments arranged by size:
2144 1183 513 384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 281 1508 138
Size: 143 1227 3270
Fragments arranged by size:
3270 1227 143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 2695 3187 3485 1816
Size: 879 492 298 2971
Fragments arranged by size:
2971 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4344 4344
Size: 4640
BsaAI yAC'GTr
Cuts at: 1993 1993
Size: 4640
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3302 3449 3860 1829
Size: 521 678 274 147 411 2609
Fragments arranged by size:
2609 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 254 284 1481 1511 254
Size: 30 1197 30 3383
Fragments arranged by size:
3383 1197 30 30
BseYI C'CCAG_C
Cuts at: 1086 2065 2823 3400 3896 1086
Size: 979 758 577 496 1830
Fragments arranged by size:
1830 979 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 836 788
Size: 48 4592
BsiEI CG_ry'CG
Cuts at: 104 2182 2228 3436 104
Size: 2078 46 1208 1308
Fragments arranged by size:
2078 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1256 1467 1654 2432 2622 3414 1256
Size: 211 187 778 190 792 2482
Fragments arranged by size:
2482 792 778 211 190 187
Bsp1286I G_dGCh'C
Cuts at: 1256 1467 1654 2265 2358 2432 2622 2684 3414
Size: 211 187 611 93 74 190 62 730
Cuts at: 3414 1256
Size: 2482
Fragments arranged by size:
2482 730 611 211 190 187 93 74
62
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 3816 3816
Size: 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1690 1904 2206 2587 1690
Size: 214 302 381 3743
Fragments arranged by size:
3743 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2552 2552
Size: 4640
BsrFI r'CCGG_y
Cuts at: 2638 2819 3028 2638
Size: 181 209 4250
Fragments arranged by size:
4250 209 181
BssSI C'ACGA_G
Cuts at: 2911 3269 2911
Size: 358 4282
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 2017 1603
Size: 414 4226
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstYI r'GATC_y
Cuts at: 1 1101 1427 1665 2132 2155 2490 2736 3748
Size: 1100 326 238 467 23 335 246 1012
Cuts at: 3748 3834 3846 4192 1
Size: 86 12 346 449
Fragments arranged by size:
1100 1012 467 449 346 335 326 246
238 86 23 12
BtgI C'CryG_G
Cuts at: 411 653 411
Size: 242 4398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2590 2771 2934 2590
Size: 181 163 4296
Fragments arranged by size:
4296 181 163
BtsI GCAGTG_nn'
Cuts at: 200 593 1883 200
Size: 393 1290 2957
Fragments arranged by size:
2957 1290 393
DraI TTT'AAA
Cuts at: 735 918 924 1639 735
Size: 183 6 715 3736
Fragments arranged by size:
3736 715 183 6
DraIII CAC_nnn'GTG
Cuts at: 337 337
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 2346 3204 2346
Size: 858 3782
EaeI y'GGCC_r
Cuts at: 140 1047 2225 2399 2790 2817 4162 140
Size: 907 1178 174 391 27 1345 618
Fragments arranged by size:
1345 1178 907 618 391 174 27
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 481 1076 1319 1334 2663 2873 481
Size: 595 243 15 1329 210 2248
Fragments arranged by size:
2248 1329 595 243 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2848 3158 3304 4450 2848
Size: 310 146 1146 3038
Fragments arranged by size:
3038 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 281 1031 1319 1508 2465 2897 3644 3912
Size: 143 750 288 189 957 432 747 268
Cuts at: 3912 138
Size: 866
Fragments arranged by size:
957 866 750 747 432 288 268 189
143
EcoO109I rG'GnC_Cy
Cuts at: 1779 1779
Size: 4640
EcoRI G'AATT_C
Cuts at: 1115 1644 1115
Size: 529 4111
EcoRV GAT'ATC
Cuts at: 878 878
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1528 1618 2659 1528
Size: 90 1041 3509
Fragments arranged by size:
3509 1041 90
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 2074 2322 3344 4323 2074
Size: 248 1022 979 2391
Fragments arranged by size:
2391 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 412 444 2482 2512 2514 2544 105
Size: 32 275 32 2038 30 2 30 2201
Fragments arranged by size:
2201 2038 275 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1679 1679
Size: 4640
HindIII A'AGCT_T
Cuts at: 503 1583 503
Size: 1080 3560
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MfeI C'AATT_G
Cuts at: 1266 1266
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 840 1594 3311 3495 840
Size: 754 1717 184 1985
Fragments arranged by size:
1985 1717 754 184
MscI TGG'CCA
Cuts at: 142 2401 142
Size: 2259 2381
MslI CAynn'nnrTG
Cuts at: 557 851 2629 2756 557
Size: 294 1778 127 2441
Fragments arranged by size:
2441 1778 294 127
MspA1I CmG'CkG
Cuts at: 1751 2065 2425 3438 3683 1751
Size: 314 360 1013 245 2708
Fragments arranged by size:
2708 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2821 2821
Size: 4640
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NcoI C'CATG_G
Cuts at: 411 411
Size: 4640
NdeI CA'TA_TG
Cuts at: 404 404
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 2819 2819
Size: 4640
NspI r_CATG'y
Cuts at: 347 1693 2193 3100 347
Size: 1346 500 907 1887
Fragments arranged by size:
1887 1346 907 500
PciI A'CATG_T
Cuts at: 3096 3096
Size: 4640
PflMI CCAn_nnn'nTGG
Cuts at: 1486 1486
Size: 4640
PfoI T'CCnGG_A
Cuts at: 258 678 258
Size: 420 4220
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PstI C_TGCA'G
Cuts at: 195 498 1687 2372 195
Size: 303 1189 685 2463
Fragments arranged by size:
2463 1189 685 303
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 2065 2425 2065
Size: 360 4280
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 1076 1319 2663 2873 1076
Size: 243 1344 210 2843
Fragments arranged by size:
2843 1344 243 210
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 492 1457 4157 492
Size: 965 2700 975
Fragments arranged by size:
2700 975 965
SexAI A'CCwGG_T
Cuts at: 1479 1479
Size: 4640
SfcI C'TryA_G
Cuts at: 33 178 191 494 716 1683 2368 3361 3552
Size: 145 13 303 222 967 685 993 191
Cuts at: 3552 33
Size: 1121
Fragments arranged by size:
1121 993 967 685 303 222 191 145
13
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 1795 2710 3202 3464 1795
Size: 915 492 262 2971
Fragments arranged by size:
2971 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
StyI C'CwwG_G
Cuts at: 137 411 1431 1774 2128 137
Size: 274 1020 343 354 2649
Fragments arranged by size:
2649 1020 354 343 274
SwaI ATTT'AAAT
Cuts at: 924 924
Size: 4640
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2223 2356 2815 3024 2223
Size: 133 459 209 3839
Fragments arranged by size:
3839 459 209 133
TatI w'GTAC_w
Cuts at: 128 490 1029 1352 1455 4155 128
Size: 362 539 323 103 2700 613
Fragments arranged by size:
2700 613 539 362 323 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4157 4189 4157
Size: 32 4608
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 430 1671 430
Size: 1241 3399
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 409 2135 409
Size: 1726 2914
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI
FspAI HpaI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI XhoI
XmnI ZraI