Restriction map for
pF1KB4985
(Circular) (Six-base) MAPSORT of: pF1KB4985.seq Check: 7714 from: 1 to: 4718
pF1KB4985 4718 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1051 1051
Size: 4718
AccI GT'mk_AC
Cuts at: 1756 1756
Size: 4718
Acc65I G'GTAC_C
Cuts at: 1734 1734
Size: 4718
AclI AA'CG_TT
Cuts at: 4558 4558
Size: 4718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1652 2543 2975 3328 3990 1652
Size: 891 432 353 662 2380
Fragments arranged by size:
2380 891 662 432 353
AflII C'TTAA_G
Cuts at: 955 955
Size: 4718
AflIII A'CryG_T
Cuts at: 1689 2070 3870 4151 1689
Size: 381 1800 281 2256
Fragments arranged by size:
2256 1800 381 281
AhdI GACnn_n'nnGTC
Cuts at: 179 179
Size: 4718
AleI CACnn'nnGTG
Cuts at: 1709 1709
Size: 4718
AlwNI CAG_nnn'CTG
Cuts at: 154 2023 3461 154
Size: 1869 1438 1411
Fragments arranged by size:
1869 1438 1411
ApaLI G'TGCA_C
Cuts at: 3556 3556
Size: 4718
ApoI r'AATT_y
Cuts at: 257 568 815 1250 1722 4071 4327 257
Size: 311 247 435 472 2349 256 648
Fragments arranged by size:
2349 648 472 435 311 256 247
AseI AT'TA_AT
Cuts at: 20 991 20
Size: 971 3747
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
AvaI C'yCGr_G
Cuts at: 869 1738 869
Size: 869 3849
BamHI G'GATC_C
Cuts at: 1743 1743
Size: 4718
BanI G'GyrC_C
Cuts at: 199 307 1237 1555 1677 1734 2148 2396 2431
Size: 108 930 318 122 57 414 248 35
Cuts at: 2431 199
Size: 2486
Fragments arranged by size:
2486 930 414 318 248 122 108 57
35
BanII G_rGCy'C
Cuts at: 1732 2762 1732
Size: 1030 3688
BbeI G_GCGC'C
Cuts at: 2152 2400 2152
Size: 248 4470
BbsI GAAGACnn'nnnn_
Cuts at: 1181 1181
Size: 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1134 1168 1134
Size: 34 4684
BciVI GTATCCnnnnn_n'
Cuts at: 834 2616 3672 4718 834
Size: 1782 1056 1046 834
Fragments arranged by size:
1782 1056 1046 834
BclI T'GATC_A
Cuts at: 546 1113 1698 2238 546
Size: 567 585 540 3026
Fragments arranged by size:
3026 585 567 540
BglI GCCn_nnn'nGGC
Cuts at: 511 511
Size: 4718
BglII A'GATC_T
Cuts at: 2233 4270 2233
Size: 2037 2681
BlpI GC'TnA_GC
Cuts at: 1830 1830
Size: 4718
Bme1580I G_kGCm'C
Cuts at: 601 2343 2436 3560 601
Size: 1742 93 1124 1759
Fragments arranged by size:
1759 1742 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1930 2011 2346 4490 1930
Size: 81 335 2144 2158
Fragments arranged by size:
2158 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 4718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 715
Size: 4718
Bpu10I CC'TnA_GC
Cuts at: 1501 1501
Size: 4718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1894 2773 3487 3785 1894
Size: 879 714 298 2827
Fragments arranged by size:
2827 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4422 4422
Size: 4718
BsaAI yAC'GTr
Cuts at: 2071 2071
Size: 4718
BsaBI GATnn'nnATC
Cuts at: 6 2232 4269 6
Size: 2226 2037 455
Fragments arranged by size:
2226 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 2149 2397 3099 4595 112
Size: 2037 248 702 1496 235
Fragments arranged by size:
2037 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1907 2428 3106 3517 3664 3938 1907
Size: 521 678 411 147 274 2687
Fragments arranged by size:
2687 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 206 236 206
Size: 30 4688
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1349 1349
Size: 4718
BseYI C'CCAG_C
Cuts at: 2143 2901 3566 3974 2143
Size: 758 665 408 2887
Fragments arranged by size:
2887 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1502 1646 1502
Size: 144 4574
BsiEI CG_ry'CG
Cuts at: 104 2260 2306 3536 104
Size: 2156 46 1230 1286
Fragments arranged by size:
2156 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 232 1732 2510 2700 3560 232
Size: 1500 778 190 860 1390
Fragments arranged by size:
1500 1390 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 232 601 1732 2343 2436 2510 2700 2762 3560
Size: 369 1131 611 93 74 190 62 798
Cuts at: 3560 232
Size: 1390
Fragments arranged by size:
1390 1131 798 611 369 190 93 74
62
BspHI T'CATG_A
Cuts at: 1163 3150 1163
Size: 1987 2731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1051 1768 1982 2284 2665 1051
Size: 717 214 302 381 3104
Fragments arranged by size:
3104 717 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3010 3064 4548 4713 50
Size: 2960 54 1484 165 55
Fragments arranged by size:
2960 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1273 1418 2630 1273
Size: 145 1212 3361
Fragments arranged by size:
3361 1212 145
BsrFI r'CCGG_y
Cuts at: 2716 2897 2716
Size: 181 4537
BssSI C'ACGA_G
Cuts at: 2989 3697 2989
Size: 708 4010
BstAPI GCAn_nnn'nTGC
Cuts at: 898 1057 2095 898
Size: 159 1038 3521
Fragments arranged by size:
3521 1038 159
BstBI TT'CG_AA
Cuts at: 3079 3079
Size: 4718
BstEII G'GTnAC_C
Cuts at: 294 294
Size: 4718
BstXI CCAn_nnnn'nTGG
Cuts at: 249 249
Size: 4718
BstYI r'GATC_y
Cuts at: 1 1743 2210 2233 2568 2814 3120 3132 3218
Size: 1742 467 23 335 246 306 12 86
Cuts at: 3218 4270 1
Size: 1052 449
Fragments arranged by size:
1742 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4718
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 189 2668 2849 3012 189
Size: 2479 181 163 1895
Fragments arranged by size:
2479 1895 181 163
BtsI GCAGTG_nn'
Cuts at: 276 1529 1961 276
Size: 1253 432 3033
Fragments arranged by size:
3033 1253 432
DraI TTT'AAA
Cuts at: 1717 1717
Size: 4718
DrdI GACnn_nn'nnGTC
Cuts at: 2424 3768 2424
Size: 1344 3374
EaeI y'GGCC_r
Cuts at: 2303 2477 2868 2895 4240 2303
Size: 174 391 27 1345 2781
Fragments arranged by size:
2781 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2303 2303
Size: 4718
EarI CTCTTCn'nnn_
Cuts at: 155 2741 2951 155
Size: 2586 210 1922
Fragments arranged by size:
2586 1922 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2926 3668 3814 4528 2926
Size: 742 146 714 3116
Fragments arranged by size:
3116 742 714 146
EcoICRI GAG'CTC
Cuts at: 1730 1730
Size: 4718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1652 2543 2975 3328 3990 715
Size: 937 891 432 353 662 1443
Fragments arranged by size:
1443 937 891 662 432 353
EcoNI CCTnn'n_nnAGG
Cuts at: 1210 1210
Size: 4718
EcoO109I rG'GnC_Cy
Cuts at: 238 1857 238
Size: 1619 3099
EcoRI G'AATT_C
Cuts at: 1722 1722
Size: 4718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 249 2737 249
Size: 2488 2230
FspI TGC'GCA
Cuts at: 1301 2499 1301
Size: 1198 3520
HaeII r_GCGC'y
Cuts at: 529 2152 2400 3630 4401 529
Size: 1623 248 1230 771 846
Fragments arranged by size:
1623 1230 846 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 165 197 2560 2590 2592 2622 165
Size: 32 2363 30 2 30 2261
Fragments arranged by size:
2363 2261 32 30 30 2
HincII GTy'rAC
Cuts at: 135 336 882 1757 135
Size: 201 546 875 3096
Fragments arranged by size:
3096 875 546 201
HindIII A'AGCT_T
Cuts at: 1132 1132
Size: 4718
HpaI GTT'AAC
Cuts at: 135 135
Size: 4718
KasI G'GCGC_C
Cuts at: 2148 2396 2148
Size: 248 4470
KpnI G_GTAC'C
Cuts at: 1738 1738
Size: 4718
MluI A'CGCG_T
Cuts at: 4151 4151
Size: 4718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 475 746 3477 3661 475
Size: 271 2731 184 1532
Fragments arranged by size:
2731 1532 271 184
MscI TGG'CCA
Cuts at: 2479 2479
Size: 4718
MslI CAynn'nnrTG
Cuts at: 1709 2707 2834 1709
Size: 998 127 3593
Fragments arranged by size:
3593 998 127
MspA1I CmG'CkG
Cuts at: 1630 1829 2143 2503 3287 3532 1630
Size: 199 314 360 784 245 2816
Fragments arranged by size:
2816 784 360 314 245 199
NaeI GCC'GGC
Cuts at: 2899 2899
Size: 4718
NarI GG'CG_CC
Cuts at: 2149 2397 2149
Size: 248 4470
NcoI C'CATG_G
Cuts at: 107 107
Size: 4718
NgoMIV G'CCGG_C
Cuts at: 2897 2897
Size: 4718
NspI r_CATG'y
Cuts at: 916 1051 1771 2271 3874 916
Size: 135 720 500 1603 1760
Fragments arranged by size:
1760 1603 720 500 135
PciI A'CATG_T
Cuts at: 3870 3870
Size: 4718
PmeI GTTT'AAAC
Cuts at: 1717 1717
Size: 4718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 3164
Size: 32 4686
PpuMI rG'GwC_Cy
Cuts at: 238 238
Size: 4718
PsiI TTA'TAA
Cuts at: 998 998
Size: 4718
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 650 682 650
Size: 32 4686
PstI C_TGCA'G
Cuts at: 543 1765 2450 543
Size: 1222 685 2811
Fragments arranged by size:
2811 1222 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4718
PvuII CAG'CTG
Cuts at: 1630 2143 2503 1630
Size: 513 360 3845
Fragments arranged by size:
3845 513 360
RsrII CG'GwC_CG
Cuts at: 2913 2913
Size: 4718
SacI G_AGCT'C
Cuts at: 1732 1732
Size: 4718
SalI G'TCGA_C
Cuts at: 1755 1755
Size: 4718
SanDI GG'GwC_CC
Cuts at: 238 238
Size: 4718
SapI GCTCTTCn'nnn_
Cuts at: 2741 2951 2741
Size: 210 4508
SbfI CC_TGCA'GG
Cuts at: 1765 1765
Size: 4718
ScaI AGT'ACT
Cuts at: 4235 4235
Size: 4718
SexAI A'CCwGG_T
Cuts at: 1487 1487
Size: 4718
SfcI C'TryA_G
Cuts at: 33 539 656 1761 2446 3414 3605 33
Size: 506 117 1105 685 968 191 1146
Fragments arranged by size:
1146 1105 968 685 506 191 117
SfoI GGC'GCC
Cuts at: 2150 2398 2150
Size: 248 4470
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
SmaI CCC'GGG
Cuts at: 871 1740 871
Size: 869 3849
SmlI C'TyrA_G
Cuts at: 955 1873 2788 3502 3764 955
Size: 918 915 714 262 1909
Fragments arranged by size:
1909 918 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4718
SphI G_CATG'C
Cuts at: 1051 1771 2271 1051
Size: 720 500 3498
Fragments arranged by size:
3498 720 500
SspI AAT'ATT
Cuts at: 591 591
Size: 4718
StyI C'CwwG_G
Cuts at: 107 233 1206 1852 2206 107
Size: 126 973 646 354 2619
Fragments arranged by size:
2619 973 646 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1133 1695 2301 2434 2893 3102 1133
Size: 562 606 133 459 209 2749
Fragments arranged by size:
2749 606 562 459 209 133
TatI w'GTAC_w
Cuts at: 4233 4233
Size: 4718
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4235 4267 4235
Size: 32 4686
Tth111I GACn'n_nGTC
Cuts at: 2515 2515
Size: 4718
XbaI T'CTAG_A
Cuts at: 1318 1749 1318
Size: 431 4287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 1536 1566 2213 216
Size: 1320 30 647 2721
Fragments arranged by size:
2721 1320 647 30
XmaI C'CCGG_G
Cuts at: 869 1738 869
Size: 869 3849
XmnI GAAnn'nnTTC
Cuts at: 665 950 1595 665
Size: 285 645 3788
Fragments arranged by size:
3788 645 285
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PspOMI
PspXI SacII SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI