Restriction map for
pF1KB4988
(Circular) (Six-base) MAPSORT of: pF1KB4988.seq Check: 1374 from: 1 to: 3872
pF1KB4988 3872 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2129 2876 3144 1697
Size: 432 747 268 2425
Fragments arranged by size:
2425 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 780 1177 2744 780
Size: 397 1567 1908
Fragments arranged by size:
1908 1567 397
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 554 876 3225 3481 554
Size: 322 2349 256 945
Fragments arranged by size:
2349 945 322 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 892 892
Size: 3872
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 888 1302 1550 1585 888
Size: 414 248 35 3175
Fragments arranged by size:
3175 414 248 35
BanII G_rGCy'C
Cuts at: 294 842 886 1916 294
Size: 548 44 1030 2250
Fragments arranged by size:
2250 1030 548 44
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BbsI GAAGACnn'nnnn_
Cuts at: 201 201
Size: 3872
BbvCI CC'TCA_GC
Cuts at: 689 689
Size: 3872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 667 701 667
Size: 34 3838
BciVI GTATCCnnnnn_n'
Cuts at: 1770 2531 3872 1770
Size: 761 1341 1770
Fragments arranged by size:
1770 1341 761
BclI T'GATC_A
Cuts at: 275 1392 275
Size: 1117 2755
BfrBI ATG'CAT
Cuts at: 738 738
Size: 3872
BglI GCCn_nnn'nGGC
Cuts at: 119 757 119
Size: 638 3234
BglII A'GATC_T
Cuts at: 1387 3424 1387
Size: 2037 1835
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 1497 1590 2646 1497
Size: 93 1056 2723
Fragments arranged by size:
2723 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 582 1084 1165 1500 3644 582
Size: 502 81 335 2144 810
Fragments arranged by size:
2144 810 502 335 81
BmtI G_CTAG'C
Cuts at: 512 512
Size: 3872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 635 2239 635
Size: 1604 2268
Bpu10I CC'TnA_GC
Cuts at: 689 689
Size: 3872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3576 3576
Size: 3872
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 191 221 191
Size: 30 3842
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 120 832 120
Size: 712 3160
BseYI C'CCAG_C
Cuts at: 780 1297 2055 2632 3128 780
Size: 517 758 577 496 1524
Fragments arranged by size:
1524 758 577 517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 831
Size: 3872
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 842 886 1664 1854 2646 842
Size: 44 778 190 792 2068
Fragments arranged by size:
2068 792 778 190 44
BsmI GAATG_Cn'
Cuts at: 461 461
Size: 3872
Bsp1286I G_dGCh'C
Cuts at: 294 842 886 1497 1590 1664 1854 1916 2646
Size: 548 44 611 93 74 190 62 730
Cuts at: 2646 294
Size: 1520
Fragments arranged by size:
1520 730 611 548 190 93 74 62
44
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 731 3048 731
Size: 2317 1555
BspMI ACCTGCnnnn'nnnn_
Cuts at: 922 1136 1438 1819 922
Size: 214 302 381 2975
Fragments arranged by size:
2975 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1784 1784
Size: 3872
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BsrGI T'GTAC_A
Cuts at: 251 528 251
Size: 277 3595
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 188 1249 188
Size: 1061 2811
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstYI r'GATC_y
Cuts at: 1 825 897 1364 1387 1722 1968 2980 3066
Size: 824 72 467 23 335 246 1012 86
Cuts at: 3066 3078 3424 1
Size: 12 346 449
Fragments arranged by size:
1012 824 467 449 346 335 246 86
72 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3872
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 1115
Size: 3872
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 1457 1631 2022 2049 3394 1457
Size: 174 391 27 1345 1935
Fragments arranged by size:
1935 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 869 2080 2390 2536 3682 869
Size: 1211 310 146 1146 1059
Fragments arranged by size:
1211 1146 1059 310 146
EcoICRI GAG'CTC
Cuts at: 840 884 840
Size: 44 3828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1697 2129 2876 3144 1697
Size: 432 747 268 2425
Fragments arranged by size:
2425 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1011 1011
Size: 3872
EcoRI G'AATT_C
Cuts at: 554 876 554
Size: 322 3550
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 1306 1554 2576 3555 1306
Size: 248 1022 979 1623
Fragments arranged by size:
1623 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 191 223 810 842 1714 1744 1746 1776 191
Size: 32 587 32 872 30 2 30 2287
Fragments arranged by size:
2287 872 587 32 32 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MfeI C'AATT_G
Cuts at: 648 648
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 701 2543 2727 701
Size: 1842 184 1846
Fragments arranged by size:
1846 1842 184
MscI TGG'CCA
Cuts at: 1633 1633
Size: 3872
MslI CAynn'nnrTG
Cuts at: 548 1861 1988 548
Size: 1313 127 2432
Fragments arranged by size:
2432 1313 127
MspA1I CmG'CkG
Cuts at: 178 540 777 983 1297 1657 2670 2915 178
Size: 362 237 206 314 360 1013 245 1135
Fragments arranged by size:
1135 1013 362 360 314 245 237 206
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NcoI C'CATG_G
Cuts at: 107 107
Size: 3872
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NheI G'CTAG_C
Cuts at: 508 508
Size: 3872
NsiI A_TGCA'T
Cuts at: 740 740
Size: 3872
NspI r_CATG'y
Cuts at: 742 789 925 1425 2332 742
Size: 47 136 500 907 2282
Fragments arranged by size:
2282 907 500 136 47
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PfoI T'CCnGG_A
Cuts at: 335 335
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PstI C_TGCA'G
Cuts at: 634 919 1604 634
Size: 285 685 2902
Fragments arranged by size:
2902 685 285
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 178 540 777 1297 1657 178
Size: 362 237 520 360 2393
Fragments arranged by size:
2393 520 362 360 237
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 842 886 842
Size: 44 3828
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 3389 3389
Size: 3872
SfcI C'TryA_G
Cuts at: 33 269 284 630 915 1600 2593 2784 33
Size: 236 15 346 285 685 993 191 1121
Fragments arranged by size:
1121 993 685 346 285 236 191 15
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 894 894
Size: 3872
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 789 925 1425 789
Size: 136 500 3236
Fragments arranged by size:
3236 500 136
StyI C'CwwG_G
Cuts at: 107 1006 1360 107
Size: 899 354 2619
Fragments arranged by size:
2619 899 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 251 528 3387 251
Size: 277 2859 736
Fragments arranged by size:
2859 736 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1367 141
Size: 1226 2646
XmaI C'CCGG_G
Cuts at: 892 892
Size: 3872
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BmgBI BpmI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI