Restriction map for
pF1KB4989
(Circular) (Six-base) MAPSORT of: pF1KB4989.seq Check: 5080 from: 1 to: 3896
pF1KB4989 3896 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 131 131
Size: 3896
AccI GT'mk_AC
Cuts at: 544 934 544
Size: 390 3506
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 890 3736 890
Size: 2846 1050
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 772 1721 2153 2900 3168 418
Size: 354 949 432 747 268 1146
Fragments arranged by size:
1146 949 747 432 354 268
AflII C'TTAA_G
Cuts at: 475 475
Size: 3896
AflIII A'CryG_T
Cuts at: 1248 2352 3329 1248
Size: 1104 977 1815
Fragments arranged by size:
1815 1104 977
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 396 783 1201 2768 396
Size: 387 418 1567 1524
Fragments arranged by size:
1567 1524 418 387
ApaLI G'TGCA_C
Cuts at: 2666 2666
Size: 3896
ApoI r'AATT_y
Cuts at: 366 412 633 646 900 3249 3505 366
Size: 46 221 13 254 2349 256 757
Fragments arranged by size:
2349 757 256 254 221 46 13
AseI AT'TA_AT
Cuts at: 20 791 20
Size: 771 3125
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 912 1326 1574 1609 912
Size: 414 248 35 3199
Fragments arranged by size:
3199 414 248 35
BanII G_rGCy'C
Cuts at: 236 910 1940 236
Size: 674 1030 2192
Fragments arranged by size:
2192 1030 674
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BbsI GAAGACnn'nnnn_
Cuts at: 427 427
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 712 1794 2555 3896 712
Size: 1082 761 1341 712
Fragments arranged by size:
1341 1082 761 712
BclI T'GATC_A
Cuts at: 702 1416 702
Size: 714 3182
BglII A'GATC_T
Cuts at: 161 1411 3448 161
Size: 1250 2037 609
Fragments arranged by size:
2037 1250 609
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 1521 1614 2670 1521
Size: 93 1056 2747
Fragments arranged by size:
2747 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 220
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2443 2741 1072
Size: 879 492 298 2227
Fragments arranged by size:
2227 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 348 1249 348
Size: 901 2995
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 484 484
Size: 3896
BseYI C'CCAG_C
Cuts at: 880 1321 2079 2656 3152 880
Size: 441 758 577 496 1624
Fragments arranged by size:
1624 758 577 496 441
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 646
Size: 3896
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 236 910 1688 1878 2670 236
Size: 674 778 190 792 1462
Fragments arranged by size:
1462 792 778 674 190
Bsp1286I G_dGCh'C
Cuts at: 236 910 1521 1614 1688 1878 1940 2670 236
Size: 674 611 93 74 190 62 730 1462
Fragments arranged by size:
1462 730 674 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 416 3072 416
Size: 2656 1240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 131 503 946 1160 1462 1843 131
Size: 372 443 214 302 381 2184
Fragments arranged by size:
2184 443 381 372 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 720 1808 720
Size: 1088 2808
BsrFI r'CCGG_y
Cuts at: 1894 2075 2284 1894
Size: 181 209 3506
Fragments arranged by size:
3506 209 181
BssSI C'ACGA_G
Cuts at: 2167 2525 2167
Size: 358 3538
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstEII G'GTnAC_C
Cuts at: 193 193
Size: 3896
BstYI r'GATC_y
Cuts at: 1 161 756 780 858 921 1388 1411 1746
Size: 160 595 24 78 63 467 23 335
Cuts at: 1746 1992 3004 3090 3102 3448 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 595 467 449 346 335 246 160
86 78 63 24 23 12
BstZ17I GTA'TAC
Cuts at: 545 545
Size: 3896
BtgI C'CryG_G
Cuts at: 107 107
Size: 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 648 683 648
Size: 35 3861
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 608 1481 1655 2046 2073 3418 608
Size: 873 174 391 27 1345 1086
Fragments arranged by size:
1345 1086 873 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 356 385 1919 2129 356
Size: 29 1534 210 2123
Fragments arranged by size:
2123 1534 210 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 234 908 234
Size: 674 3222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 418 772 1721 2153 2900 3168 220
Size: 198 354 949 432 747 268 948
Fragments arranged by size:
949 948 747 432 354 268 198
EcoO109I rG'GnC_Cy
Cuts at: 492 1035 492
Size: 543 3353
EcoRI G'AATT_C
Cuts at: 412 900 412
Size: 488 3408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2600 3579 1330
Size: 248 1022 979 1647
Fragments arranged by size:
1647 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1738 1768 1770 1800 1738
Size: 30 2 30 3834
Fragments arranged by size:
3834 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 812 2567 2751 812
Size: 1755 184 1957
Fragments arranged by size:
1957 1755 184
MscI TGG'CCA
Cuts at: 610 1657 610
Size: 1047 2849
MslI CAynn'nnrTG
Cuts at: 1885 2012 1885
Size: 127 3769
MspA1I CmG'CkG
Cuts at: 396 1007 1321 1681 2694 2939 396
Size: 611 314 360 1013 245 1353
Fragments arranged by size:
1353 1013 611 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NcoI C'CATG_G
Cuts at: 107 107
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PfoI T'CCnGG_A
Cuts at: 227 227
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 492 492
Size: 3896
PstI C_TGCA'G
Cuts at: 128 218 943 1628 128
Size: 90 725 685 2396
Fragments arranged by size:
2396 725 685 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 396 1321 1681 396
Size: 925 360 2611
Fragments arranged by size:
2611 925 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 236 910 236
Size: 674 3222
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 385 1919 2129 385
Size: 1534 210 2152
Fragments arranged by size:
2152 1534 210
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 124 214 939 1624 2617 2808 33
Size: 91 90 725 685 993 191 1121
Fragments arranged by size:
1121 993 725 685 191 91 90
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 475 1051 1966 2458 2720 475
Size: 576 915 492 262 1651
Fragments arranged by size:
1651 915 576 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
SspI AAT'ATT
Cuts at: 798 798
Size: 3896
StyI C'CwwG_G
Cuts at: 107 1030 1384 107
Size: 923 354 2619
Fragments arranged by size:
2619 923 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
XmnI GAAnn'nnTTC
Cuts at: 676 676
Size: 3896
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstXI
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI