Restriction map for
pF1KB4991
(Circular) (Six-base) MAPSORT of: pF1KB4991.seq Check: 2100 from: 1 to: 4046
pF1KB4991 4046 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 454 1084 454
Size: 630 3416
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 644 1871 2303 3050 3318 460
Size: 184 1227 432 747 268 1188
Fragments arranged by size:
1227 1188 747 432 268 184
AflIII A'CryG_T
Cuts at: 1398 2502 3479 1398
Size: 1104 977 1965
Fragments arranged by size:
1965 1104 977
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 223 223
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 418 1050 3399 3655 418
Size: 632 2349 256 809
Fragments arranged by size:
2349 809 632 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 183 1066 183
Size: 883 3163
BamHI G'GATC_C
Cuts at: 1071 1071
Size: 4046
BanI G'GyrC_C
Cuts at: 563 1062 1476 1724 1759 563
Size: 499 414 248 35 2850
Fragments arranged by size:
2850 499 414 248 35
BanII G_rGCy'C
Cuts at: 777 900 1060 2090 777
Size: 123 160 1030 2733
Fragments arranged by size:
2733 1030 160 123
BbeI G_GCGC'C
Cuts at: 567 1480 1728 567
Size: 913 248 2885
Fragments arranged by size:
2885 913 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4012
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 809 1566 809
Size: 757 3289
BglI GCCn_nnn'nGGC
Cuts at: 904 904
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 1158 1158
Size: 4046
Bme1580I G_kGCm'C
Cuts at: 1671 1764 2820 1671
Size: 93 1056 2897
Fragments arranged by size:
2897 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 860 1258 1339 1674 3818 860
Size: 398 81 335 2144 1088
Fragments arranged by size:
2144 1088 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 603 875 1222 2101 2593 2891 603
Size: 272 347 879 492 298 1758
Fragments arranged by size:
1758 879 492 347 298 272
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 474 1399 474
Size: 925 3121
BsaBI GATnn'nnATC
Cuts at: 6 1560 3597 6
Size: 1554 2037 455
Fragments arranged by size:
2037 1554 455
BsaHI Gr'CG_yC
Cuts at: 564 1477 1725 2427 3923 564
Size: 913 248 702 1496 687
Fragments arranged by size:
1496 913 702 687 248
BsaWI w'CCGG_w
Cuts at: 425 980 1235 1756 2434 2708 2855 3266 425
Size: 555 255 521 678 274 147 411 1205
Fragments arranged by size:
1205 678 555 521 411 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 887 917 887
Size: 30 4016
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 204 785 201
Size: 3 581 3462
Fragments arranged by size:
3462 581 3
BseYI C'CCAG_C
Cuts at: 1471 2229 2806 3302 1471
Size: 758 577 496 2215
Fragments arranged by size:
2215 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 137 454 1588 1634 2842 104
Size: 33 317 1134 46 1208 1308
Fragments arranged by size:
1308 1208 1134 317 46 33
BsiHKAI G_wGCw'C
Cuts at: 371 1060 1838 2028 2820 371
Size: 689 778 190 792 1597
Fragments arranged by size:
1597 792 778 689 190
Bsp1286I G_dGCh'C
Cuts at: 371 777 900 1060 1671 1764 1838 2028 2090
Size: 406 123 160 611 93 74 190 62
Cuts at: 2090 2820 371
Size: 730 1597
Fragments arranged by size:
1597 730 611 406 190 160 123 93
74 62
BspEI T'CCGG_A
Cuts at: 980 3266 980
Size: 2286 1760
BspHI T'CATG_A
Cuts at: 812 3222 812
Size: 2410 1636
BspMI ACCTGCnnnn'nnnn_
Cuts at: 755 1096 1310 1612 1993 755
Size: 341 214 302 381 2808
Fragments arranged by size:
2808 381 341 302 214
BsrBI CCG'CTC
Cuts at: 50 194 2338 2392 3876 4041 50
Size: 144 2144 54 1484 165 55
Fragments arranged by size:
2144 1484 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 318 389 542 1958 318
Size: 71 153 1416 2406
Fragments arranged by size:
2406 1416 153 71
BsrFI r'CCGG_y
Cuts at: 2044 2225 2434 2044
Size: 181 209 3656
Fragments arranged by size:
3656 209 181
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 4046
BssSI C'ACGA_G
Cuts at: 2317 2675 2317
Size: 358 3688
BstAPI GCAn_nnn'nTGC
Cuts at: 886 1423 886
Size: 537 3509
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstYI r'GATC_y
Cuts at: 1 428 1071 1538 1561 1896 2142 3154 3240
Size: 427 643 467 23 335 246 1012 86
Cuts at: 3240 3252 3598 1
Size: 12 346 449
Fragments arranged by size:
1012 643 467 449 427 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 377 377
Size: 4046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 667 1752 2610 667
Size: 1085 858 2103
Fragments arranged by size:
2103 1085 858
EaeI y'GGCC_r
Cuts at: 699 1631 1805 2196 2223 3568 699
Size: 932 174 391 27 1345 1177
Fragments arranged by size:
1345 1177 932 391 174 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 235 244 943 2069 2279 235
Size: 9 699 1126 210 2002
Fragments arranged by size:
2002 1126 699 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 644 1871 2303 3050 3318 460
Size: 184 1227 432 747 268 1188
Fragments arranged by size:
1227 1188 747 432 268 184
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 418 1050 418
Size: 632 3414
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 708 2065 708
Size: 1357 2689
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 172 567 1480 1728 2750 3729 172
Size: 395 913 248 1022 979 489
Fragments arranged by size:
1022 979 913 489 395 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1888 1918 1920 1950 1888
Size: 30 2 30 3984
Fragments arranged by size:
3984 30 30 2
HincII GTy'rAC
Cuts at: 455 1085 455
Size: 630 3416
KasI G'GCGC_C
Cuts at: 563 1476 1724 563
Size: 913 248 2885
Fragments arranged by size:
2885 913 248
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2717 2901 2717
Size: 184 3862
MscI TGG'CCA
Cuts at: 701 1807 701
Size: 1106 2940
MslI CAynn'nnrTG
Cuts at: 866 2035 2162 866
Size: 1169 127 2750
Fragments arranged by size:
2750 1169 127
MspA1I CmG'CkG
Cuts at: 1157 1471 1831 2844 3089 1157
Size: 314 360 1013 245 2114
Fragments arranged by size:
2114 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 564 1477 1725 564
Size: 913 248 2885
Fragments arranged by size:
2885 913 248
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NruI TCG'CGA
Cuts at: 403 403
Size: 4046
NspI r_CATG'y
Cuts at: 1099 1599 2506 1099
Size: 500 907 2639
Fragments arranged by size:
2639 907 500
PciI A'CATG_T
Cuts at: 2502 2502
Size: 4046
PfoI T'CCnGG_A
Cuts at: 140 671 140
Size: 531 3515
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 752 800 1093 1778 752
Size: 48 293 685 3020
Fragments arranged by size:
3020 685 293 48
PvuI CG_AT'CG
Cuts at: 104 137 104
Size: 33 4013
PvuII CAG'CTG
Cuts at: 1471 1831 1471
Size: 360 3686
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 453 1083 453
Size: 630 3416
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 752 1093 752
Size: 341 3705
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 642 748 796 1089 1774 2767 2958 33
Size: 609 106 48 293 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 293 191 106 48
SfoI GGC'GCC
Cuts at: 565 1478 1726 565
Size: 913 248 2885
Fragments arranged by size:
2885 913 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 582 890 1201 2116 2608 2870 582
Size: 308 311 915 492 262 1758
Fragments arranged by size:
1758 915 492 311 308 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
StyI C'CwwG_G
Cuts at: 1180 1534 1180
Size: 354 3692
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1629 1762 2221 2430 1629
Size: 133 459 209 3245
Fragments arranged by size:
3245 459 209 133
TatI w'GTAC_w
Cuts at: 719 3561 719
Size: 2842 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 887 919 3563 3595 887
Size: 32 2644 32 1338
Fragments arranged by size:
2644 1338 32 32
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 873 1541 873
Size: 668 3378
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
XmnI GAAnn'nnTTC
Cuts at: 688 688
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
BpmI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI