Restriction map for
pF1KB4992
(Circular) (Six-base) MAPSORT of: pF1KB4992.seq Check: 6749 from: 1 to: 3956
pF1KB4992 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 592 592
Size: 3956
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 1781 2213 2960 3228 771
Size: 1010 432 747 268 1499
Fragments arranged by size:
1499 1010 747 432 268
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AleI CACnn'nnGTG
Cuts at: 133 133
Size: 3956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 255 255
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 1261 2828 1261
Size: 1567 2389
ApaLI G'TGCA_C
Cuts at: 274 2726 274
Size: 2452 1504
ApoI r'AATT_y
Cuts at: 406 445 710 960 3309 3565 406
Size: 39 265 250 2349 256 797
Fragments arranged by size:
2349 797 265 256 250 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 976 976
Size: 3956
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 972 1386 1634 1669 972
Size: 414 248 35 3259
Fragments arranged by size:
3259 414 248 35
BanII G_rGCy'C
Cuts at: 812 970 2000 812
Size: 158 1030 2768
Fragments arranged by size:
2768 1030 158
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BbsI GAAGACnn'nnnn_
Cuts at: 941 941
Size: 3956
BciVI GTATCCnnnnn_n'
Cuts at: 1854 2615 3956 1854
Size: 761 1341 1854
Fragments arranged by size:
1854 1341 761
BclI T'GATC_A
Cuts at: 534 1476 534
Size: 942 3014
BglII A'GATC_T
Cuts at: 539 1471 3508 539
Size: 932 2037 987
Fragments arranged by size:
2037 987 932
BlpI GC'TnA_GC
Cuts at: 631 1068 631
Size: 437 3519
Bme1580I G_kGCm'C
Cuts at: 278 1581 1674 2730 278
Size: 1303 93 1056 1504
Fragments arranged by size:
1504 1303 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 784
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 1132 2011 2503 2801 300
Size: 832 879 492 298 1455
Fragments arranged by size:
1455 879 832 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3660 3660
Size: 3956
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 589 1387 1635 2337 3833 589
Size: 798 248 702 1496 712
Fragments arranged by size:
1496 798 712 702 248
BsaWI w'CCGG_w
Cuts at: 659 1145 1666 2344 2618 2765 3176 659
Size: 486 521 678 274 147 411 1439
Fragments arranged by size:
1439 678 521 486 411 274 147
BseYI C'CCAG_C
Cuts at: 1381 2139 2716 3212 1381
Size: 758 577 496 2125
Fragments arranged by size:
2125 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 3956
BsiEI CG_ry'CG
Cuts at: 104 1498 1544 2752 104
Size: 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 278 812 970 1748 1938 2730 278
Size: 534 158 778 190 792 1504
Fragments arranged by size:
1504 792 778 534 190 158
BsmI GAATG_Cn'
Cuts at: 653 653
Size: 3956
Bsp1286I G_dGCh'C
Cuts at: 278 812 970 1581 1674 1748 1938 2000 2730
Size: 534 158 611 93 74 190 62 730
Cuts at: 2730 278
Size: 1504
Fragments arranged by size:
1504 730 611 534 190 158 93 74
62
BspEI T'CCGG_A
Cuts at: 659 3176 659
Size: 2517 1439
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1220 1522 1903 1006
Size: 214 302 381 3059
Fragments arranged by size:
3059 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 642 1868 642
Size: 1226 2730
BsrFI r'CCGG_y
Cuts at: 626 1954 2135 2344 626
Size: 1328 181 209 2238
Fragments arranged by size:
2238 1328 209 181
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 1333 1333
Size: 3956
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstYI r'GATC_y
Cuts at: 1 539 981 1448 1471 1806 2052 3064 3150
Size: 538 442 467 23 335 246 1012 86
Cuts at: 3150 3162 3508 1
Size: 12 346 449
Fragments arranged by size:
1012 538 467 449 442 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 902 902
Size: 3956
BtgI C'CryG_G
Cuts at: 107 107
Size: 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 1199 1199
Size: 3956
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 110 624 690 1541 1715 2106 2133 3478 110
Size: 514 66 851 174 391 27 1345 588
Fragments arranged by size:
1345 851 588 514 391 174 66 27
EagI C'GGCC_G
Cuts at: 1541 1541
Size: 3956
EarI CTCTTCn'nnn_
Cuts at: 265 914 1979 2189 265
Size: 649 1065 210 2032
Fragments arranged by size:
2032 1065 649 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 810 968 810
Size: 158 3798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 784 1781 2213 2960 3228 771
Size: 13 997 432 747 268 1499
Fragments arranged by size:
1499 997 747 432 268 13
EcoNI CCTnn'n_nnAGG
Cuts at: 527 527
Size: 3956
EcoO109I rG'GnC_Cy
Cuts at: 1095 1095
Size: 3956
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 465 1975 465
Size: 1510 2446
FspI TGC'GCA
Cuts at: 1737 1737
Size: 3956
HaeII r_GCGC'y
Cuts at: 517 1390 1638 2660 3639 517
Size: 873 248 1022 979 834
Fragments arranged by size:
1022 979 873 834 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1828 1830 1860 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 30 30 2
HincII GTy'rAC
Cuts at: 180 369 995 180
Size: 189 626 3141
Fragments arranged by size:
3141 626 189
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 1717 1717
Size: 3956
MslI CAynn'nnrTG
Cuts at: 133 253 830 1945 2072 133
Size: 120 577 1115 127 2017
Fragments arranged by size:
2017 1115 577 127 120
MspA1I CmG'CkG
Cuts at: 732 1067 1381 1741 2754 2999 732
Size: 335 314 360 1013 245 1689
Fragments arranged by size:
1689 1013 360 335 314 245
NaeI GCC'GGC
Cuts at: 628 2137 628
Size: 1509 2447
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NcoI C'CATG_G
Cuts at: 107 107
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 626 2135 626
Size: 1509 2447
NspI r_CATG'y
Cuts at: 258 615 1009 1509 2416 258
Size: 357 394 500 907 1798
Fragments arranged by size:
1798 907 500 394 357
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PflMI CCAn_nnn'nTGG
Cuts at: 249 732 249
Size: 483 3473
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PstI C_TGCA'G
Cuts at: 1003 1688 1003
Size: 685 3271
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 732 1381 1741 732
Size: 649 360 2947
Fragments arranged by size:
2947 649 360
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 812 970 812
Size: 158 3798
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 265 914 1979 2189 265
Size: 649 1065 210 2032
Fragments arranged by size:
2032 1065 649 210
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 383 999 1684 2677 2868 33
Size: 350 616 685 993 191 1121
Fragments arranged by size:
1121 993 685 616 350 191
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 978 978
Size: 3956
SmlI C'TyrA_G
Cuts at: 279 1111 2026 2518 2780 279
Size: 832 915 492 262 1455
Fragments arranged by size:
1455 915 832 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StyI C'CwwG_G
Cuts at: 107 1090 1444 107
Size: 983 354 2619
Fragments arranged by size:
2619 983 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 352 1539 1672 2131 2340 352
Size: 1187 133 459 209 1968
Fragments arranged by size:
1968 1187 459 209 133
TatI w'GTAC_w
Cuts at: 122 3471 122
Size: 3349 607
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 561 593 3473 3505 561
Size: 32 2880 32 1012
Fragments arranged by size:
2880 1012 32 32
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1451 1451
Size: 3956
XmaI C'CCGG_G
Cuts at: 976 976
Size: 3956
ZraI GAC'GTC
Cuts at: 590 590
Size: 3956
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI