Restriction map for
pF1KB4995
(Circular) (Six-base) MAPSORT of: pF1KB4995.seq Check: 7416 from: 1 to: 5135
pF1KB4995 5135 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 747 747
Size: 5135
AccI GT'mk_AC
Cuts at: 158 2173 158
Size: 2015 3120
Acc65I G'GTAC_C
Cuts at: 2151 2151
Size: 5135
AclI AA'CG_TT
Cuts at: 4975 4975
Size: 5135
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2960 3392 3745 4407 2960
Size: 432 353 662 3688
Fragments arranged by size:
3688 662 432 353
AfeI AGC'GCT
Cuts at: 451 451
Size: 5135
AflIII A'CryG_T
Cuts at: 2127 2487 4287 4568 2127
Size: 360 1800 281 2694
Fragments arranged by size:
2694 1800 360 281
AgeI A'CCGG_T
Cuts at: 1460 1460
Size: 5135
AleI CACnn'nnGTG
Cuts at: 1436 1436
Size: 5135
AlwNI CAG_nnn'CTG
Cuts at: 228 504 1135 1598 1807 2440 3878 228
Size: 276 631 463 209 633 1438 1485
Fragments arranged by size:
1485 1438 633 631 463 276 209
ApaI G_GGCC'C
Cuts at: 1502 1502
Size: 5135
ApaLI G'TGCA_C
Cuts at: 256 3973 256
Size: 3717 1418
ApoI r'AATT_y
Cuts at: 2139 4488 4744 2139
Size: 2349 256 2530
Fragments arranged by size:
2530 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5135
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
AvaI C'yCGr_G
Cuts at: 894 1065 1206 1266 1448 2155 894
Size: 171 141 60 182 707 3874
Fragments arranged by size:
3874 707 182 171 141 60
BamHI G'GATC_C
Cuts at: 2160 2160
Size: 5135
BanI G'GyrC_C
Cuts at: 1568 2151 2565 2813 2848 1568
Size: 583 414 248 35 3855
Fragments arranged by size:
3855 583 414 248 35
BanII G_rGCy'C
Cuts at: 295 1447 1502 1702 2149 3179 295
Size: 1152 55 200 447 1030 2251
Fragments arranged by size:
2251 1152 1030 447 200 55
BbeI G_GCGC'C
Cuts at: 2569 2817 2569
Size: 248 4887
BbsI GAAGACnn'nnnn_
Cuts at: 1949 1949
Size: 5135
BbvCI CC'TCA_GC
Cuts at: 943 1606 943
Size: 663 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 103 137 138 172 337 371 103
Size: 34 1 34 165 34 4867
Fragments arranged by size:
4867 165 34 34 34 1
BciVI GTATCCnnnnn_n'
Cuts at: 3033 4089 5135 3033
Size: 1056 1046 3033
Fragments arranged by size:
3033 1056 1046
BclI T'GATC_A
Cuts at: 2655 2655
Size: 5135
BglI GCCn_nnn'nGGC
Cuts at: 1482 1482
Size: 5135
BglII A'GATC_T
Cuts at: 512 765 2650 4687 512
Size: 253 1885 2037 960
Fragments arranged by size:
2037 1885 960 253
BlpI GC'TnA_GC
Cuts at: 2247 2247
Size: 5135
Bme1580I G_kGCm'C
Cuts at: 260 360 1502 2760 2853 3977 260
Size: 100 1142 1258 93 1124 1418
Fragments arranged by size:
1418 1258 1142 1124 100 93
BmgBI CAC'GTC
Cuts at: 723 735 723
Size: 12 5123
BmrI ACTGGGnnnn_n'
Cuts at: 216 2347 2428 2763 4907 216
Size: 2131 81 335 2144 444
Fragments arranged by size:
2144 2131 444 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 705 1047 1059 3502 705
Size: 342 12 2443 2338
Fragments arranged by size:
2443 2338 342 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1260 1272 1284 526
Size: 734 12 12 4377
Fragments arranged by size:
4377 734 12 12
Bpu10I CC'TnA_GC
Cuts at: 943 1606 943
Size: 663 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 2079 2311 3190 3904 4202 892
Size: 1187 232 879 714 298 1825
Fragments arranged by size:
1825 1187 879 714 298 232
BsaI GGTCTCn'nnnn_
Cuts at: 540 4839 540
Size: 4299 836
BsaAI yAC'GTr
Cuts at: 2488 2488
Size: 5135
BsaBI GATnn'nnATC
Cuts at: 6 2649 4686 6
Size: 2643 2037 455
Fragments arranged by size:
2643 2037 455
BsaHI Gr'CG_yC
Cuts at: 900 912 1825 2566 2814 3516 5012 900
Size: 12 913 741 248 702 1496 1023
Fragments arranged by size:
1496 1023 913 741 702 248 12
BsaWI w'CCGG_w
Cuts at: 824 1460 2324 2845 3523 3934 4081 4355 824
Size: 636 864 521 678 411 147 274 1604
Fragments arranged by size:
1604 864 678 636 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 1339 1369 1450 1480 649
Size: 30 660 30 81 30 4304
Fragments arranged by size:
4304 660 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 508 670 673 676 691 694 853 889 892
Size: 162 3 3 15 3 159 36 3
Cuts at: 892 1090 1132 1227 1230 1233 1236 1239 1242
Size: 198 42 95 3 3 3 3 3
Cuts at: 1242 1245 508
Size: 3 4398
Fragments arranged by size:
4398 198 162 159 95 42 36 15
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 216 550 967 1187 1654 1660 2560 3318 3983
Size: 334 417 220 467 6 900 758 665
Cuts at: 3983 4391 216
Size: 408 960
Fragments arranged by size:
960 900 758 665 467 417 408 334
220 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 487
Size: 5135
BsiEI CG_ry'CG
Cuts at: 104 674 1344 1557 1742 2677 2723 3953 104
Size: 570 670 213 185 935 46 1230 1286
Fragments arranged by size:
1286 1230 935 670 570 213 185 46
BsiHKAI G_wGCw'C
Cuts at: 260 2149 2927 3117 3977 260
Size: 1889 778 190 860 1418
Fragments arranged by size:
1889 1418 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 177 1445 177
Size: 1268 3867
Bsp1286I G_dGCh'C
Cuts at: 260 295 360 1447 1502 1702 2149 2760 2853
Size: 35 65 1087 55 200 447 611 93
Cuts at: 2853 2927 3117 3179 3977 260
Size: 74 190 62 798 1418
Fragments arranged by size:
1418 1087 798 611 447 200 190 93
74 65 62 55 35
BspEI T'CCGG_A
Cuts at: 824 824
Size: 5135
BspHI T'CATG_A
Cuts at: 3567 3567
Size: 5135
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 747 1791 2185 2399 2701 3082 222
Size: 525 1044 394 214 302 381 2275
Fragments arranged by size:
2275 1044 525 394 381 302 214
BsrBI CCG'CTC
Cuts at: 50 135 180 905 1043 1049 1055 1235 1286
Size: 85 45 725 138 6 6 180 51
Cuts at: 1286 1310 1355 1367 1379 1385 1391 1472 1523
Size: 24 45 12 12 6 6 81 51
Cuts at: 1523 1929 1938 1986 2045 3427 3481 4965 5130
Size: 406 9 48 59 1382 54 1484 165
Cuts at: 5130 50
Size: 55
Fragments arranged by size:
1484 1382 725 406 180 165 138 85
81 59 55 54 51 51 48 45
45 24 12 12 9 6 6 6
6
BsrDI GCAATG_nn'
Cuts at: 3047 3047
Size: 5135
BsrFI r'CCGG_y
Cuts at: 481 1424 1460 1737 2033 3133 3314 481
Size: 943 36 277 296 1100 181 2302
Fragments arranged by size:
2302 1100 943 296 277 181 36
BssSI C'ACGA_G
Cuts at: 115 3406 4114 115
Size: 3291 708 1136
Fragments arranged by size:
3291 1136 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 2512 1135
Size: 1377 3758
BstBI TT'CG_AA
Cuts at: 3496 3496
Size: 5135
BstEII G'GTnAC_C
Cuts at: 1013 1013
Size: 5135
BstYI r'GATC_y
Cuts at: 1 512 765 1970 2160 2627 2650 2985 3231
Size: 511 253 1205 190 467 23 335 246
Cuts at: 3231 3537 3549 3635 4687 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1205 1052 511 467 449 335 306 253
246 190 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1564 1829 1564
Size: 265 4870
BtgI C'CryG_G
Cuts at: 1317 1419 1958 1317
Size: 102 539 4494
Fragments arranged by size:
4494 539 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1127 3085 3266 3429 1127
Size: 1958 181 163 2833
Fragments arranged by size:
2833 1958 181 163
BtsI GCAGTG_nn'
Cuts at: 505 2378 505
Size: 1873 3262
DraI TTT'AAA
Cuts at: 2134 2134
Size: 5135
DrdI GACnn_nn'nnGTC
Cuts at: 2841 4185 2841
Size: 1344 3791
EaeI y'GGCC_r
Cuts at: 240 281 623 671 797 1341 1422 1554 1739
Size: 41 342 48 126 544 81 132 185
Cuts at: 1739 2720 2894 3285 3312 4657 240
Size: 981 174 391 27 1345 718
Fragments arranged by size:
1345 981 718 544 391 342 185 174
132 126 81 48 41 27
EagI C'GGCC_G
Cuts at: 671 1341 1554 1739 2720 671
Size: 670 213 185 981 3086
Fragments arranged by size:
3086 981 670 213 185
EarI CTCTTCn'nnn_
Cuts at: 163 1263 2006 2085 3158 3368 163
Size: 1100 743 79 1073 210 1930
Fragments arranged by size:
1930 1100 1073 743 210 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 1897 3343 4085 4231 4945 190
Size: 1707 1446 742 146 714 380
Fragments arranged by size:
1707 1446 742 714 380 146
EcoICRI GAG'CTC
Cuts at: 2147 2147
Size: 5135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1260 1272 1284 2960 3392 3745 4407 526
Size: 734 12 12 1676 432 353 662 1254
Fragments arranged by size:
1676 1254 734 662 432 353 12 12
EcoO109I rG'GnC_Cy
Cuts at: 213 1271 1499 1560 2099 2274 213
Size: 1058 228 61 539 175 3074
Fragments arranged by size:
3074 1058 539 228 175 61
EcoRI G'AATT_C
Cuts at: 2139 2139
Size: 5135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1966 3154 1966
Size: 1188 3947
FseI GG_CCGG'CC
Cuts at: 1741 1741
Size: 5135
FspI TGC'GCA
Cuts at: 2916 2916
Size: 5135
HaeII r_GCGC'y
Cuts at: 453 1226 1401 1864 2094 2569 2817 4047 4818
Size: 773 175 463 230 475 248 1230 771
Cuts at: 4818 453
Size: 770
Fragments arranged by size:
1230 773 771 770 475 463 248 230
175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 649 681 2977 3007 3009 3039 335
Size: 32 282 32 2296 30 2 30 2431
Fragments arranged by size:
2431 2296 282 32 32 30 30 2
HincII GTy'rAC
Cuts at: 159 2174 159
Size: 2015 3120
HindIII A'AGCT_T
Cuts at: 139 139
Size: 5135
KasI G'GCGC_C
Cuts at: 2565 2813 2565
Size: 248 4887
KpnI G_GTAC'C
Cuts at: 2155 2155
Size: 5135
MluI A'CGCG_T
Cuts at: 4568 4568
Size: 5135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 3894 4078 404
Size: 3490 184 1461
Fragments arranged by size:
3490 1461 184
MscI TGG'CCA
Cuts at: 2896 2896
Size: 5135
MslI CAynn'nnrTG
Cuts at: 721 1436 3124 3251 721
Size: 715 1688 127 2605
Fragments arranged by size:
2605 1688 715 127
MspA1I CmG'CkG
Cuts at: 286 670 918 1135 1598 1789 2246 2560 2920
Size: 384 248 217 463 191 457 314 360
Cuts at: 2920 3704 3949 286
Size: 784 245 1472
Fragments arranged by size:
1472 784 463 457 384 360 314 248
245 217 191
NaeI GCC'GGC
Cuts at: 483 1426 1739 2035 3316 483
Size: 943 313 296 1281 2302
Fragments arranged by size:
2302 1281 943 313 296
NarI GG'CG_CC
Cuts at: 2566 2814 2566
Size: 248 4887
NcoI C'CATG_G
Cuts at: 1958 1958
Size: 5135
NgoMIV G'CCGG_C
Cuts at: 481 1424 1737 2033 3314 481
Size: 943 313 296 1281 2302
Fragments arranged by size:
2302 1281 943 313 296
NspI r_CATG'y
Cuts at: 862 2131 2188 2688 4291 862
Size: 1269 57 500 1603 1706
Fragments arranged by size:
1706 1603 1269 500 57
PciI A'CATG_T
Cuts at: 2127 4287 2127
Size: 2160 2975
PmeI GTTT'AAAC
Cuts at: 2134 2134
Size: 5135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 5103
PpuMI rG'GwC_Cy
Cuts at: 213 1271 2099 213
Size: 1058 828 3249
Fragments arranged by size:
3249 1058 828
PspOMI G'GGCC_C
Cuts at: 1498 1498
Size: 5135
PspXI vC'TCGA_Gb
Cuts at: 1266 1266
Size: 5135
PstI C_TGCA'G
Cuts at: 233 273 2182 2867 233
Size: 40 1909 685 2501
Fragments arranged by size:
2501 1909 685 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5135
PvuII CAG'CTG
Cuts at: 1598 2560 2920 1598
Size: 962 360 3813
Fragments arranged by size:
3813 962 360
RsrII CG'GwC_CG
Cuts at: 3330 3330
Size: 5135
SacI G_AGCT'C
Cuts at: 2149 2149
Size: 5135
SalI G'TCGA_C
Cuts at: 157 2172 157
Size: 2015 3120
SapI GCTCTTCn'nnn_
Cuts at: 2085 3158 3368 2085
Size: 1073 210 3852
Fragments arranged by size:
3852 1073 210
SbfI CC_TGCA'GG
Cuts at: 233 2182 233
Size: 1949 3186
ScaI AGT'ACT
Cuts at: 4652 4652
Size: 5135
SfcI C'TryA_G
Cuts at: 33 229 269 453 1542 2178 2863 3831 4022
Size: 196 40 184 1089 636 685 968 191
Cuts at: 4022 33
Size: 1146
Fragments arranged by size:
1146 1089 968 685 636 196 191 184
40
SfoI GGC'GCC
Cuts at: 2567 2815 2567
Size: 248 4887
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5135
SmaI CCC'GGG
Cuts at: 1067 1208 2157 1067
Size: 141 949 4045
Fragments arranged by size:
4045 949 141
SmlI C'TyrA_G
Cuts at: 871 1266 2094 2290 3205 3919 4181 871
Size: 395 828 196 915 714 262 1825
Fragments arranged by size:
1825 915 828 714 395 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5135
SphI G_CATG'C
Cuts at: 862 2188 2688 862
Size: 1326 500 3309
Fragments arranged by size:
3309 1326 500
StuI AGG'CCT
Cuts at: 461 539 461
Size: 78 5057
StyI C'CwwG_G
Cuts at: 863 1958 2269 2623 863
Size: 1095 311 354 3375
Fragments arranged by size:
3375 1095 354 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 356 981 1792 2718 2851 3310 3519 356
Size: 625 811 926 133 459 209 1972
Fragments arranged by size:
1972 926 811 625 459 209 133
TatI w'GTAC_w
Cuts at: 890 2027 4650 890
Size: 1137 2623 1375
Fragments arranged by size:
2623 1375 1137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1339 1371 1450 1482 4652 4684 1339
Size: 32 79 32 3170 32 1790
Fragments arranged by size:
3170 1790 79 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 811 2932 811
Size: 2121 3014
XbaI T'CTAG_A
Cuts at: 2166 2166
Size: 5135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2630 2630
Size: 5135
XhoI C'TCGA_G
Cuts at: 1266 1266
Size: 5135
XmaI C'CCGG_G
Cuts at: 1065 1206 2155 1065
Size: 141 949 4045
Fragments arranged by size:
4045 949 141
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI