Restriction map for
pF1KB4998
(Circular) (Six-base) MAPSORT of: pF1KB4998.seq Check: 9932 from: 1 to: 3776
pF1KB4998 3776 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 583 814 583
Size: 231 3545
Acc65I G'GTAC_C
Cuts at: 792 792
Size: 3776
AclI AA'CG_TT
Cuts at: 3616 3616
Size: 3776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 767 1601 2033 2386 3048 767
Size: 834 432 353 662 1495
Fragments arranged by size:
1495 834 662 432 353
AflIII A'CryG_T
Cuts at: 233 1128 2928 3209 233
Size: 895 1800 281 800
Fragments arranged by size:
1800 895 800 281
AlwNI CAG_nnn'CTG
Cuts at: 1081 2519 1081
Size: 1438 2338
ApaLI G'TGCA_C
Cuts at: 2614 2614
Size: 3776
ApoI r'AATT_y
Cuts at: 780 3129 3385 780
Size: 2349 256 1171
Fragments arranged by size:
2349 1171 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
AvaI C'yCGr_G
Cuts at: 796 796
Size: 3776
AvrII C'CTAG_G
Cuts at: 472 472
Size: 3776
BamHI G'GATC_C
Cuts at: 801 801
Size: 3776
BanI G'GyrC_C
Cuts at: 792 1206 1454 1489 792
Size: 414 248 35 3079
Fragments arranged by size:
3079 414 248 35
BanII G_rGCy'C
Cuts at: 790 1820 790
Size: 1030 2746
BbeI G_GCGC'C
Cuts at: 1210 1458 1210
Size: 248 3528
BbsI GAAGACnn'nnnn_
Cuts at: 198 198
Size: 3776
BbvCI CC'TCA_GC
Cuts at: 196 311 196
Size: 115 3661
BciVI GTATCCnnnnn_n'
Cuts at: 1674 2730 3776 1674
Size: 1056 1046 1674
Fragments arranged by size:
1674 1056 1046
BclI T'GATC_A
Cuts at: 1296 1296
Size: 3776
BglII A'GATC_T
Cuts at: 640 1291 3328 640
Size: 651 2037 1088
Fragments arranged by size:
2037 1088 651
BlpI GC'TnA_GC
Cuts at: 888 888
Size: 3776
Bme1580I G_kGCm'C
Cuts at: 1401 1494 2618 1401
Size: 93 1124 2559
Fragments arranged by size:
2559 1124 93
BmgBI CAC'GTC
Cuts at: 234 234
Size: 3776
BmrI ACTGGGnnnn_n'
Cuts at: 682 988 1069 1404 3548 682
Size: 306 81 335 2144 910
Fragments arranged by size:
2144 910 335 306 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 3776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 621
Size: 3776
Bpu10I CC'TnA_GC
Cuts at: 196 311 196
Size: 115 3661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 952 1831 2545 2843 139
Size: 813 879 714 298 1072
Fragments arranged by size:
1072 879 813 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 524 639 671 3480 524
Size: 115 32 2809 820
Fragments arranged by size:
2809 820 115 32
BsaAI yAC'GTr
Cuts at: 1129 1129
Size: 3776
BsaBI GATnn'nnATC
Cuts at: 6 1290 3327 6
Size: 1284 2037 455
Fragments arranged by size:
2037 1284 455
BsaHI Gr'CG_yC
Cuts at: 456 480 1207 1455 2157 3653 456
Size: 24 727 248 702 1496 579
Fragments arranged by size:
1496 727 702 579 248 24
BsaWI w'CCGG_w
Cuts at: 965 1486 2164 2575 2722 2996 965
Size: 521 678 411 147 274 1745
Fragments arranged by size:
1745 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1201 1959 2624 3032 1201
Size: 758 665 408 1945
Fragments arranged by size:
1945 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 120 354 387 583 724 1318 1364 2594
Size: 16 234 33 196 141 594 46 1230
Cuts at: 2594 104
Size: 1286
Fragments arranged by size:
1286 1230 594 234 196 141 46 33
16
BsiHKAI G_wGCw'C
Cuts at: 790 1568 1758 2618 790
Size: 778 190 860 1948
Fragments arranged by size:
1948 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 790 1401 1494 1568 1758 1820 2618 790
Size: 611 93 74 190 62 798 1948
Fragments arranged by size:
1948 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2208 2208
Size: 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 826 1040 1342 1723 422
Size: 404 214 302 381 2475
Fragments arranged by size:
2475 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 545 760 2068 2122 3606 3771 50
Size: 495 215 1308 54 1484 165 55
Fragments arranged by size:
1484 1308 495 215 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1688 1688
Size: 3776
BsrFI r'CCGG_y
Cuts at: 1774 1955 1774
Size: 181 3595
BsrGI T'GTAC_A
Cuts at: 236 236
Size: 3776
BssHII G'CGCG_C
Cuts at: 376 376
Size: 3776
BssSI C'ACGA_G
Cuts at: 2047 2755 2047
Size: 708 3068
BstAPI GCAn_nnn'nTGC
Cuts at: 1153 1153
Size: 3776
BstBI TT'CG_AA
Cuts at: 2137 2137
Size: 3776
BstYI r'GATC_y
Cuts at: 1 598 640 801 1268 1291 1626 1872 2178
Size: 597 42 161 467 23 335 246 306
Cuts at: 2178 2190 2276 3328 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 597 467 449 335 306 246 161
86 42 23 12
BtgI C'CryG_G
Cuts at: 244 270 329 244
Size: 26 59 3691
Fragments arranged by size:
3691 59 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1726 1907 2070 1726
Size: 181 163 3432
Fragments arranged by size:
3432 181 163
BtsI GCAGTG_nn'
Cuts at: 1019 1019
Size: 3776
DraI TTT'AAA
Cuts at: 775 775
Size: 3776
DrdI GACnn_nn'nnGTC
Cuts at: 591 1482 2826 591
Size: 891 1344 1541
Fragments arranged by size:
1541 1344 891
EaeI y'GGCC_r
Cuts at: 117 351 384 1361 1535 1926 1953 3298 117
Size: 234 33 977 174 391 27 1345 595
Fragments arranged by size:
1345 977 595 391 234 174 33 27
EagI C'GGCC_G
Cuts at: 117 351 384 1361 117
Size: 234 33 977 2532
Fragments arranged by size:
2532 977 234 33
EarI CTCTTCn'nnn_
Cuts at: 721 743 1799 2009 721
Size: 22 1056 210 2488
Fragments arranged by size:
2488 1056 210 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 2726 2872 3586 1984
Size: 742 146 714 2174
Fragments arranged by size:
2174 742 714 146
EcoICRI GAG'CTC
Cuts at: 788 788
Size: 3776
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 767 1601 2033 2386 3048 621
Size: 146 834 432 353 662 1349
Fragments arranged by size:
1349 834 662 432 353 146
EcoO109I rG'GnC_Cy
Cuts at: 659 915 659
Size: 256 3520
EcoRI G'AATT_C
Cuts at: 780 780
Size: 3776
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1795 1795
Size: 3776
FspI TGC'GCA
Cuts at: 1557 1557
Size: 3776
HaeII r_GCGC'y
Cuts at: 1210 1458 2688 3459 1210
Size: 248 1230 771 1527
Fragments arranged by size:
1527 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1618 1648 1650 1680 1618
Size: 30 2 30 3714
Fragments arranged by size:
3714 30 30 2
HincII GTy'rAC
Cuts at: 584 815 584
Size: 231 3545
KasI G'GCGC_C
Cuts at: 1206 1454 1206
Size: 248 3528
KpnI G_GTAC'C
Cuts at: 796 796
Size: 3776
MluI A'CGCG_T
Cuts at: 3209 3209
Size: 3776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 484 2535 2719 484
Size: 2051 184 1541
Fragments arranged by size:
2051 1541 184
MscI TGG'CCA
Cuts at: 1537 1537
Size: 3776
MslI CAynn'nnrTG
Cuts at: 1765 1892 1765
Size: 127 3649
MspA1I CmG'CkG
Cuts at: 246 272 887 1201 1561 2345 2590 246
Size: 26 615 314 360 784 245 1432
Fragments arranged by size:
1432 784 615 360 314 245 26
NaeI GCC'GGC
Cuts at: 1957 1957
Size: 3776
NarI GG'CG_CC
Cuts at: 1207 1455 1207
Size: 248 3528
NcoI C'CATG_G
Cuts at: 329 329
Size: 3776
NgoMIV G'CCGG_C
Cuts at: 1955 1955
Size: 3776
NruI TCG'CGA
Cuts at: 720 720
Size: 3776
NspI r_CATG'y
Cuts at: 829 1329 2932 829
Size: 500 1603 1673
Fragments arranged by size:
1673 1603 500
PciI A'CATG_T
Cuts at: 2928 2928
Size: 3776
PfoI T'CCnGG_A
Cuts at: 496 673 496
Size: 177 3599
PmeI GTTT'AAAC
Cuts at: 775 775
Size: 3776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2254 2222
Size: 32 3744
PpuMI rG'GwC_Cy
Cuts at: 659 659
Size: 3776
PstI C_TGCA'G
Cuts at: 823 1508 823
Size: 685 3091
PvuI CG_AT'CG
Cuts at: 104 724 104
Size: 620 3156
PvuII CAG'CTG
Cuts at: 1201 1561 1201
Size: 360 3416
RsrII CG'GwC_CG
Cuts at: 1971 1971
Size: 3776
SacI G_AGCT'C
Cuts at: 790 790
Size: 3776
SacII CC_GC'GG
Cuts at: 247 273 247
Size: 26 3750
SalI G'TCGA_C
Cuts at: 582 813 582
Size: 231 3545
SapI GCTCTTCn'nnn_
Cuts at: 1799 2009 1799
Size: 210 3566
SbfI CC_TGCA'GG
Cuts at: 823 823
Size: 3776
ScaI AGT'ACT
Cuts at: 3293 3293
Size: 3776
SfcI C'TryA_G
Cuts at: 33 537 819 1504 2472 2663 33
Size: 504 282 685 968 191 1146
Fragments arranged by size:
1146 968 685 504 282 191
SfoI GGC'GCC
Cuts at: 1208 1456 1208
Size: 248 3528
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3776
SmaI CCC'GGG
Cuts at: 798 798
Size: 3776
SmlI C'TyrA_G
Cuts at: 154 931 1846 2560 2822 154
Size: 777 915 714 262 1108
Fragments arranged by size:
1108 915 777 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3776
SphI G_CATG'C
Cuts at: 829 1329 829
Size: 500 3276
StyI C'CwwG_G
Cuts at: 260 329 472 910 1264 260
Size: 69 143 438 354 2772
Fragments arranged by size:
2772 438 354 143 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 545 569 621 659 1359 1492 1951 2160 545
Size: 24 52 38 700 133 459 209 2161
Fragments arranged by size:
2161 700 459 209 133 52 38 24
TatI w'GTAC_w
Cuts at: 236 3291 236
Size: 3055 721
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 3744
Tth111I GACn'n_nGTC
Cuts at: 1573 1573
Size: 3776
XbaI T'CTAG_A
Cuts at: 807 807
Size: 3776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1271 1271
Size: 3776
XmaI C'CCGG_G
Cuts at: 796 796
Size: 3776
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI BaeI BcgI BfrBI BglI BmtI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI