Restriction map for
pF1KB5000
(Circular) (Six-base) MAPSORT of: pF1KB5000.seq Check: 811 from: 1 to: 3725
pF1KB5000 3725 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1550 1982 2729 2997 385
Size: 1165 432 747 268 1113
Fragments arranged by size:
1165 1113 747 432 268
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 1030 2597 1030
Size: 1567 2158
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 456 490 729 3078 3334 456
Size: 34 239 2349 256 847
Fragments arranged by size:
2349 847 256 239 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 745 745
Size: 3725
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 741 1155 1403 1438 741
Size: 414 248 35 3028
Fragments arranged by size:
3028 414 248 35
BanII G_rGCy'C
Cuts at: 225 444 739 1769 225
Size: 219 295 1030 2181
Fragments arranged by size:
2181 1030 295 219
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglI GCCn_nnn'nGGC
Cuts at: 260 314 260
Size: 54 3671
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 837 837
Size: 3725
Bme1580I G_kGCm'C
Cuts at: 1350 1443 2499 1350
Size: 93 1056 2576
Fragments arranged by size:
2576 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 268 322 474 546 937 1018 1353 3497 268
Size: 54 152 72 391 81 335 2144 496
Fragments arranged by size:
2144 496 391 335 152 81 72 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 3725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 685
Size: 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 1078 1078
Size: 3725
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 429 1156 1404 2106 3602 429
Size: 727 248 702 1496 552
Fragments arranged by size:
1496 727 702 552 248
BsaWI w'CCGG_w
Cuts at: 914 1435 2113 2387 2534 2945 914
Size: 521 678 274 147 411 1694
Fragments arranged by size:
1694 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 430
Size: 3725
BseYI C'CCAG_C
Cuts at: 1150 1908 2485 2981 1150
Size: 758 577 496 1894
Fragments arranged by size:
1894 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 739 1517 1707 2499 739
Size: 778 190 792 1965
Fragments arranged by size:
1965 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 225 444 739 1350 1443 1517 1707 1769 2499
Size: 219 295 611 93 74 190 62 730
Cuts at: 2499 225
Size: 1451
Fragments arranged by size:
1451 730 611 295 219 190 93 74
62
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 775 989 1291 1672 775
Size: 214 302 381 2828
Fragments arranged by size:
2828 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2017 2071 3555 3720 50
Size: 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 1637
Size: 3725
BsrFI r'CCGG_y
Cuts at: 1723 1904 2113 1723
Size: 181 209 3335
Fragments arranged by size:
3335 209 181
BssHII G'CGCG_C
Cuts at: 250 250
Size: 3725
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1102
Size: 3725
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstXI CCAn_nnnn'nTGG
Cuts at: 658 658
Size: 3725
BstYI r'GATC_y
Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931
Size: 749 467 23 335 246 1012 86 12
Cuts at: 2931 3277 1
Size: 346 449
Fragments arranged by size:
1012 749 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 335 335
Size: 3725
BtgI C'CryG_G
Cuts at: 107 309 621 107
Size: 202 312 3211
Fragments arranged by size:
3211 312 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 968 968
Size: 3725
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DraIII CAC_nnn'GTG
Cuts at: 554 554
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 315 1310 1484 1875 1902 3247 315
Size: 995 174 391 27 1345 793
Fragments arranged by size:
1345 995 793 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 685 1550 1982 2729 2997 385
Size: 300 865 432 747 268 1113
Fragments arranged by size:
1113 865 747 432 300 268
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 3725
EcoO109I rG'GnC_Cy
Cuts at: 864 864
Size: 3725
EcoRI G'AATT_C
Cuts at: 456 729 456
Size: 273 3452
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 1159 1407 2429 3408 1159
Size: 248 1022 979 1476
Fragments arranged by size:
1476 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1567 1597 1599 1629 1567
Size: 30 2 30 3663
Fragments arranged by size:
3663 30 30 2
HincII GTy'rAC
Cuts at: 375 764 375
Size: 389 3336
HpaI GTT'AAC
Cuts at: 375 375
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2580 2396
Size: 184 3541
MscI TGG'CCA
Cuts at: 1486 1486
Size: 3725
MslI CAynn'nnrTG
Cuts at: 368 1714 1841 368
Size: 1346 127 2252
Fragments arranged by size:
2252 1346 127
MspA1I CmG'CkG
Cuts at: 185 836 1150 1510 2523 2768 185
Size: 651 314 360 1013 245 1142
Fragments arranged by size:
1142 1013 651 360 314 245
NaeI GCC'GGC
Cuts at: 1906 1906
Size: 3725
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NcoI C'CATG_G
Cuts at: 107 107
Size: 3725
NgoMIV G'CCGG_C
Cuts at: 1904 1904
Size: 3725
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PfoI T'CCnGG_A
Cuts at: 128 128
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2864 2896 2864
Size: 32 3693
PstI C_TGCA'G
Cuts at: 609 772 1457 609
Size: 163 685 2877
Fragments arranged by size:
2877 685 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 1150 1510 1150
Size: 360 3365
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 605 768 1453 2446 2637 33
Size: 572 163 685 993 191 1121
Fragments arranged by size:
1121 993 685 572 191 163
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 747 747
Size: 3725
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 107 627 859 1213 107
Size: 520 232 354 2619
Fragments arranged by size:
2619 520 354 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 565 1308 1441 1900 2109 100
Size: 465 743 133 459 209 1716
Fragments arranged by size:
1716 743 465 459 209 133
TatI w'GTAC_w
Cuts at: 122 3240 122
Size: 3118 607
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 3693
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 655 658 1220 655
Size: 3 562 3160
Fragments arranged by size:
3160 562 3
XmaI C'CCGG_G
Cuts at: 745 745
Size: 3725
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI