Restriction map for
pF1KB5005
(Circular) (Six-base) MAPSORT of: pF1KB5005.seq Check: 4239 from: 1 to: 4367
pF1KB5005 4367 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 246 997 2192 2624 3371 3639 246
Size: 751 1195 432 747 268 974
Fragments arranged by size:
1195 974 751 747 432 268
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 631 631
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 196 1672 3239 196
Size: 1476 1567 1324
Fragments arranged by size:
1567 1476 1324
ApaLI G'TGCA_C
Cuts at: 427 3137 427
Size: 2710 1657
ApoI r'AATT_y
Cuts at: 745 1371 3720 3976 745
Size: 626 2349 256 1136
Fragments arranged by size:
2349 1136 626 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 664 940 1387 664
Size: 276 447 3644
Fragments arranged by size:
3644 447 276
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 697 1319 1383 1797 2045 2080 697
Size: 622 64 414 248 35 2984
Fragments arranged by size:
2984 622 414 248 64 35
BanII G_rGCy'C
Cuts at: 220 347 1381 2411 220
Size: 127 1034 1030 2176
Fragments arranged by size:
2176 1034 1030 127
BbeI G_GCGC'C
Cuts at: 701 1323 1801 2049 701
Size: 622 478 248 3019
Fragments arranged by size:
3019 622 478 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglI GCCn_nnn'nGGC
Cuts at: 256 256
Size: 4367
BglII A'GATC_T
Cuts at: 980 1882 3919 980
Size: 902 2037 1428
Fragments arranged by size:
2037 1428 902
BlpI GC'TnA_GC
Cuts at: 397 1479 397
Size: 1082 3285
Bme1580I G_kGCm'C
Cuts at: 431 1992 2085 3141 431
Size: 1561 93 1056 1657
Fragments arranged by size:
1657 1561 1056 93
BmgBI CAC'GTC
Cuts at: 681 681
Size: 4367
BmrI ACTGGGnnnn_n'
Cuts at: 744 1579 1660 1995 4139 744
Size: 835 81 335 2144 972
Fragments arranged by size:
2144 972 835 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 885 166
Size: 719 3648
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 2422 2914 3212 1543
Size: 879 492 298 2698
Fragments arranged by size:
2698 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 930 1720 930
Size: 790 3577
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 376 698 1320 1798 2046 2748 4244 376
Size: 322 622 478 248 702 1496 499
Fragments arranged by size:
1496 702 622 499 478 322 248
BsaWI w'CCGG_w
Cuts at: 821 1556 2077 2755 3029 3176 3587 821
Size: 735 521 678 274 147 411 1601
Fragments arranged by size:
1601 735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 883 913 883
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 436 1243 436
Size: 807 3560
BseYI C'CCAG_C
Cuts at: 339 354 495 517 1072 1077 1792 2550 3127
Size: 15 141 22 555 5 715 758 577
Cuts at: 3127 3623 339
Size: 496 1083
Fragments arranged by size:
1083 758 715 577 555 496 141 22
15 5
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 431 581 1381 2159 2349 3141 431
Size: 150 800 778 190 792 1657
Fragments arranged by size:
1657 800 792 778 190 150
BsmBI CGTCTCn'nnnn_
Cuts at: 1346 1346
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 220 347 431 581 1381 1992 2085 2159 2349
Size: 127 84 150 800 611 93 74 190
Cuts at: 2349 2411 3141 220
Size: 62 730 1446
Fragments arranged by size:
1446 800 730 611 190 150 127 93
84 74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 809 3543 809
Size: 2734 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 186 1064 1417 1631 1933 2314 186
Size: 878 353 214 302 381 2239
Fragments arranged by size:
2239 878 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 250 433 2365 2546 2755 250
Size: 183 1932 181 209 1862
Fragments arranged by size:
1932 1862 209 183 181
BssHII G'CGCG_C
Cuts at: 475 611 475
Size: 136 4231
BssSI C'ACGA_G
Cuts at: 346 2638 2996 346
Size: 2292 358 1717
Fragments arranged by size:
2292 1717 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstEII G'GTnAC_C
Cuts at: 669 1044 669
Size: 375 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 681 1035 681
Size: 354 4013
BstYI r'GATC_y
Cuts at: 1 980 1392 1859 1882 2217 2463 3475 3561
Size: 979 412 467 23 335 246 1012 86
Cuts at: 3561 3573 3919 1
Size: 12 346 449
Fragments arranged by size:
1012 979 467 449 412 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1334 1334
Size: 4367
BtgI C'CryG_G
Cuts at: 278 320 371 470 771 800 278
Size: 42 51 99 301 29 3845
Fragments arranged by size:
3845 301 99 51 42 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 200 302 398 1610 200
Size: 102 96 1212 2957
Fragments arranged by size:
2957 1212 102 96
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1158 1193 1158
Size: 35 4332
DraI TTT'AAA
Cuts at: 1366 1366
Size: 4367
DrdI GACnn_nn'nnGTC
Cuts at: 375 2073 2931 375
Size: 1698 858 1811
Fragments arranged by size:
1811 1698 858
EaeI y'GGCC_r
Cuts at: 158 227 281 1151 1952 2126 2517 2544 3889
Size: 69 54 870 801 174 391 27 1345
Cuts at: 3889 158
Size: 636
Fragments arranged by size:
1345 870 801 636 391 174 69 54
27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 246 885 997 2192 2624 3371 3639 166
Size: 80 639 112 1195 432 747 268 894
Fragments arranged by size:
1195 894 747 639 432 268 112 80
EcoNI CCTnn'n_nnAGG
Cuts at: 145 1087 145
Size: 942 3425
EcoO109I rG'GnC_Cy
Cuts at: 1140 1506 1140
Size: 366 4001
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 609 1131 2386 609
Size: 522 1255 2590
Fragments arranged by size:
2590 1255 522
FspI TGC'GCA
Cuts at: 550 1237 2148 550
Size: 687 911 2769
Fragments arranged by size:
2769 911 687
HaeII r_GCGC'y
Cuts at: 134 251 404 701 948 1323 1801 2049 3071
Size: 117 153 297 247 375 478 248 1022
Cuts at: 3071 4050 134
Size: 979 451
Fragments arranged by size:
1022 979 478 451 375 297 248 247
153 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2209 2239 2241 2271 2209
Size: 30 2 30 4305
Fragments arranged by size:
4305 30 30 2
HincII GTy'rAC
Cuts at: 711 1406 711
Size: 695 3672
KasI G'GCGC_C
Cuts at: 697 1319 1797 2045 697
Size: 622 478 248 3019
Fragments arranged by size:
3019 622 478 248
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1163 3038 3222 1163
Size: 1875 184 2308
Fragments arranged by size:
2308 1875 184
MscI TGG'CCA
Cuts at: 283 1153 2128 283
Size: 870 975 2522
Fragments arranged by size:
2522 975 870
MslI CAynn'nnrTG
Cuts at: 310 737 814 916 2356 2483 310
Size: 427 77 102 1440 127 2194
Fragments arranged by size:
2194 1440 427 127 102 77
MspA1I CmG'CkG
Cuts at: 411 975 1478 1792 2152 3165 3410 411
Size: 564 503 314 360 1013 245 1368
Fragments arranged by size:
1368 1013 564 503 360 314 245
NaeI GCC'GGC
Cuts at: 252 435 2548 252
Size: 183 2113 2071
Fragments arranged by size:
2113 2071 183
NarI GG'CG_CC
Cuts at: 698 1320 1798 2046 698
Size: 622 478 248 3019
Fragments arranged by size:
3019 622 478 248
NcoI C'CATG_G
Cuts at: 278 278
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 250 433 2546 250
Size: 183 2113 2071
Fragments arranged by size:
2113 2071 183
NspI r_CATG'y
Cuts at: 1128 1420 1920 2827 1128
Size: 292 500 907 2668
Fragments arranged by size:
2668 907 500 292
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PflMI CCAn_nnn'nTGG
Cuts at: 279 297 759 930 279
Size: 18 462 171 3716
Fragments arranged by size:
3716 462 171 18
PfoI T'CCnGG_A
Cuts at: 143 143
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PmlI CAC'GTG
Cuts at: 930 930
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PpuMI rG'GwC_Cy
Cuts at: 1140 1140
Size: 4367
PstI C_TGCA'G
Cuts at: 183 620 1061 1414 2099 183
Size: 437 441 353 685 2451
Fragments arranged by size:
2451 685 441 437 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 411 975 1792 2152 411
Size: 564 817 360 2626
Fragments arranged by size:
2626 817 564 360
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 179 616 1057 1410 2095 3088 3279 33
Size: 146 437 441 353 685 993 191 1121
Fragments arranged by size:
1121 993 685 441 437 353 191 146
SfoI GGC'GCC
Cuts at: 699 1321 1799 2047 699
Size: 622 478 248 3019
Fragments arranged by size:
3019 622 478 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 940 1522 2437 2929 3191 940
Size: 582 915 492 262 2116
Fragments arranged by size:
2116 915 582 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StuI AGG'CCT
Cuts at: 830 830
Size: 4367
StyI C'CwwG_G
Cuts at: 221 278 284 386 674 1028 1501 1855 221
Size: 57 6 102 288 354 473 354 2733
Fragments arranged by size:
2733 473 354 354 288 102 57 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 779 793 1306 1950 2083 2542 2751 779
Size: 14 513 644 133 459 209 2395
Fragments arranged by size:
2395 644 513 459 209 133 14
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 883 915 3884 3916 883
Size: 32 2969 32 1334
Fragments arranged by size:
2969 1334 32 32
Tth111I GACn'n_nGTC
Cuts at: 789 2164 789
Size: 1375 2992
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1035 1862 1035
Size: 827 3540
XhoI C'TCGA_G
Cuts at: 940 940
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I
BsgI BsiWI BsmI BsrGI BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI