Restriction map for
pF1KB5006
(Circular) (Six-base) MAPSORT of: pF1KB5006.seq Check: 4601 from: 1 to: 4490
pF1KB5006 4490 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 433 433
Size: 4490
AccI GT'mk_AC
Cuts at: 1528 1528
Size: 4490
Acc65I G'GTAC_C
Cuts at: 1506 1506
Size: 4490
AclI AA'CG_TT
Cuts at: 4330 4330
Size: 4490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 2315 2747 3100 3762 229
Size: 2086 432 353 662 957
Fragments arranged by size:
2086 957 662 432 353
AflIII A'CryG_T
Cuts at: 1842 3642 3923 1842
Size: 1800 281 2409
Fragments arranged by size:
2409 1800 281
AleI CACnn'nnGTG
Cuts at: 922 922
Size: 4490
AlwNI CAG_nnn'CTG
Cuts at: 1795 3233 1795
Size: 1438 3052
ApaLI G'TGCA_C
Cuts at: 3328 3328
Size: 4490
ApoI r'AATT_y
Cuts at: 334 891 1494 3843 4099 334
Size: 557 603 2349 256 725
Fragments arranged by size:
2349 725 603 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4490
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
AvaI C'yCGr_G
Cuts at: 1322 1510 1322
Size: 188 4302
BamHI G'GATC_C
Cuts at: 1304 1515 1304
Size: 211 4279
BanI G'GyrC_C
Cuts at: 153 1506 1920 2168 2203 153
Size: 1353 414 248 35 2440
Fragments arranged by size:
2440 1353 414 248 35
BanII G_rGCy'C
Cuts at: 671 1504 2534 671
Size: 833 1030 2627
Fragments arranged by size:
2627 1030 833
BbeI G_GCGC'C
Cuts at: 157 1924 2172 157
Size: 1767 248 2475
Fragments arranged by size:
2475 1767 248
BbvCI CC'TCA_GC
Cuts at: 1364 1364
Size: 4490
BciVI GTATCCnnnnn_n'
Cuts at: 2388 3444 4490 2388
Size: 1056 1046 2388
Fragments arranged by size:
2388 1056 1046
BclI T'GATC_A
Cuts at: 2010 2010
Size: 4490
BglI GCCn_nnn'nGGC
Cuts at: 1198 1198
Size: 4490
BglII A'GATC_T
Cuts at: 528 2005 4042 528
Size: 1477 2037 976
Fragments arranged by size:
2037 1477 976
BlpI GC'TnA_GC
Cuts at: 593 830 1602 593
Size: 237 772 3481
Fragments arranged by size:
3481 772 237
Bme1580I G_kGCm'C
Cuts at: 232 940 2115 2208 3332 232
Size: 708 1175 93 1124 1390
Fragments arranged by size:
1390 1175 1124 708 93
BmrI ACTGGGnnnn_n'
Cuts at: 1702 1783 2118 4262 1702
Size: 81 335 2144 1930
Fragments arranged by size:
2144 1930 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1413 1481 2857 1413
Size: 68 1376 3046
Fragments arranged by size:
3046 1376 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 739 297
Size: 442 4048
Bpu10I CC'TnA_GC
Cuts at: 1364 1364
Size: 4490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 838 1666 2545 3259 3557 754
Size: 84 828 879 714 298 1687
Fragments arranged by size:
1687 879 828 714 298 84
BsaI GGTCTCn'nnnn_
Cuts at: 1397 4194 1397
Size: 2797 1693
BsaAI yAC'GTr
Cuts at: 1843 1843
Size: 4490
BsaBI GATnn'nnATC
Cuts at: 6 2004 4041 6
Size: 1998 2037 455
Fragments arranged by size:
2037 1998 455
BsaHI Gr'CG_yC
Cuts at: 154 1921 2169 2871 4367 154
Size: 1767 248 702 1496 277
Fragments arranged by size:
1767 1496 702 277 248
BsaWI w'CCGG_w
Cuts at: 1679 2200 2878 3289 3436 3710 1679
Size: 521 678 411 147 274 2459
Fragments arranged by size:
2459 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 322 352 322
Size: 30 4460
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 1381 1384 1447 133
Size: 1248 3 63 3176
Fragments arranged by size:
3176 1248 63 3
BseYI C'CCAG_C
Cuts at: 775 1280 1915 2673 3338 3746 775
Size: 505 635 758 665 408 1519
Fragments arranged by size:
1519 758 665 635 505 408
BsiEI CG_ry'CG
Cuts at: 104 134 2032 2078 3308 104
Size: 30 1898 46 1230 1286
Fragments arranged by size:
1898 1286 1230 46 30
BsiHKAI G_wGCw'C
Cuts at: 671 919 1504 2282 2472 3332 671
Size: 248 585 778 190 860 1829
Fragments arranged by size:
1829 860 778 585 248 190
Bsp1286I G_dGCh'C
Cuts at: 232 671 919 940 1504 2115 2208 2282 2472
Size: 439 248 21 564 611 93 74 190
Cuts at: 2472 2534 3332 232
Size: 62 798 1390
Fragments arranged by size:
1390 798 611 564 439 248 190 93
74 62 21
BspHI T'CATG_A
Cuts at: 2922 2922
Size: 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 433 1540 1754 2056 2437 433
Size: 1107 214 302 381 2486
Fragments arranged by size:
2486 1107 381 302 214
BsrBI CCG'CTC
Cuts at: 50 140 933 2782 2836 4320 4485 50
Size: 90 793 1849 54 1484 165 55
Fragments arranged by size:
1849 1484 793 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 380 2402 380
Size: 2022 2468
BsrFI r'CCGG_y
Cuts at: 2488 2669 2488
Size: 181 4309
BssSI C'ACGA_G
Cuts at: 2761 3469 2761
Size: 708 3782
BstAPI GCAn_nnn'nTGC
Cuts at: 1867 1867
Size: 4490
BstBI TT'CG_AA
Cuts at: 2851 2851
Size: 4490
BstXI CCAn_nnnn'nTGG
Cuts at: 280 402 280
Size: 122 4368
BstYI r'GATC_y
Cuts at: 1 528 702 1304 1515 1982 2005 2340 2586
Size: 527 174 602 211 467 23 335 246
Cuts at: 2586 2892 2904 2990 4042 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 602 527 467 449 335 306 246
211 174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1398 1398
Size: 4490
BtgI C'CryG_G
Cuts at: 164 164
Size: 4490
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2440 2621 2784 2440
Size: 181 163 4146
Fragments arranged by size:
4146 181 163
BtsI GCAGTG_nn'
Cuts at: 798 1733 798
Size: 935 3555
DraI TTT'AAA
Cuts at: 1489 1489
Size: 4490
DrdI GACnn_nn'nnGTC
Cuts at: 2196 3540 2196
Size: 1344 3146
EaeI y'GGCC_r
Cuts at: 1082 1199 2075 2249 2640 2667 4012 1082
Size: 117 876 174 391 27 1345 1560
Fragments arranged by size:
1560 1345 876 391 174 117 27
EagI C'GGCC_G
Cuts at: 2075 2075
Size: 4490
EarI CTCTTCn'nnn_
Cuts at: 533 893 1180 1435 2513 2723 533
Size: 360 287 255 1078 210 2300
Fragments arranged by size:
2300 1078 360 287 255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 566 1168 2698 3440 3586 4300 566
Size: 602 1530 742 146 714 756
Fragments arranged by size:
1530 756 742 714 602 146
EcoICRI GAG'CTC
Cuts at: 669 1502 669
Size: 833 3657
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 297 739 2315 2747 3100 3762 229
Size: 68 442 1576 432 353 662 957
Fragments arranged by size:
1576 957 662 442 432 353 68
EcoO109I rG'GnC_Cy
Cuts at: 1053 1140 1170 1242 1629 1053
Size: 87 30 72 387 3914
Fragments arranged by size:
3914 387 87 72 30
EcoRI G'AATT_C
Cuts at: 891 1494 891
Size: 603 3887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1191 2509 1191
Size: 1318 3172
FspI TGC'GCA
Cuts at: 2271 2271
Size: 4490
HaeII r_GCGC'y
Cuts at: 157 1924 2172 3402 4173 157
Size: 1767 248 1230 771 474
Fragments arranged by size:
1767 1230 771 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 164 196 254 286 322 354 2332 2362 2364
Size: 32 58 32 36 32 1978 30 2
Cuts at: 2364 2394 164
Size: 30 2260
Fragments arranged by size:
2260 1978 58 36 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1529 1529
Size: 4490
HindIII A'AGCT_T
Cuts at: 971 971
Size: 4490
KasI G'GCGC_C
Cuts at: 153 1920 2168 153
Size: 1767 248 2475
Fragments arranged by size:
2475 1767 248
KpnI G_GTAC'C
Cuts at: 1510 1510
Size: 4490
MluI A'CGCG_T
Cuts at: 3923 3923
Size: 4490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3249 3433 3249
Size: 184 4306
MscI TGG'CCA
Cuts at: 1084 2251 1084
Size: 1167 3323
MslI CAynn'nnrTG
Cuts at: 400 922 1423 2479 2606 400
Size: 522 501 1056 127 2284
Fragments arranged by size:
2284 1056 522 501 127
MspA1I CmG'CkG
Cuts at: 159 663 1284 1302 1601 1915 2275 3059 3304
Size: 504 621 18 299 314 360 784 245
Cuts at: 3304 159
Size: 1345
Fragments arranged by size:
1345 784 621 504 360 314 299 245
18
NaeI GCC'GGC
Cuts at: 2671 2671
Size: 4490
NarI GG'CG_CC
Cuts at: 154 1921 2169 154
Size: 1767 248 2475
Fragments arranged by size:
2475 1767 248
NcoI C'CATG_G
Cuts at: 164 164
Size: 4490
NgoMIV G'CCGG_C
Cuts at: 2669 2669
Size: 4490
NspI r_CATG'y
Cuts at: 1543 2043 3646 1543
Size: 500 1603 2387
Fragments arranged by size:
2387 1603 500
PciI A'CATG_T
Cuts at: 3642 3642
Size: 4490
PmeI GTTT'AAAC
Cuts at: 1489 1489
Size: 4490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 4458
PpuMI rG'GwC_Cy
Cuts at: 1242 1242
Size: 4490
PshAI GACnn'nnGTC
Cuts at: 314 314
Size: 4490
PstI C_TGCA'G
Cuts at: 1250 1537 2222 1250
Size: 287 685 3518
Fragments arranged by size:
3518 685 287
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4490
PvuII CAG'CTG
Cuts at: 1284 1915 2275 1284
Size: 631 360 3499
Fragments arranged by size:
3499 631 360
RsrII CG'GwC_CG
Cuts at: 2685 2685
Size: 4490
SacI G_AGCT'C
Cuts at: 671 1504 671
Size: 833 3657
SalI G'TCGA_C
Cuts at: 1527 1527
Size: 4490
SapI GCTCTTCn'nnn_
Cuts at: 1435 2513 2723 1435
Size: 1078 210 3202
Fragments arranged by size:
3202 1078 210
SbfI CC_TGCA'GG
Cuts at: 1537 1537
Size: 4490
ScaI AGT'ACT
Cuts at: 4007 4007
Size: 4490
SfcI C'TryA_G
Cuts at: 33 979 1102 1246 1533 2218 3186 3377 33
Size: 946 123 144 287 685 968 191 1146
Fragments arranged by size:
1146 968 946 685 287 191 144 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 1198 1198
Size: 4490
SfoI GGC'GCC
Cuts at: 155 1922 2170 155
Size: 1767 248 2475
Fragments arranged by size:
2475 1767 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
SmaI CCC'GGG
Cuts at: 1324 1512 1324
Size: 188 4302
SmlI C'TyrA_G
Cuts at: 769 853 1645 2560 3274 3536 769
Size: 84 792 915 714 262 1723
Fragments arranged by size:
1723 915 792 714 262 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4490
SphI G_CATG'C
Cuts at: 1543 2043 1543
Size: 500 3990
StuI AGG'CCT
Cuts at: 1108 1108
Size: 4490
StyI C'CwwG_G
Cuts at: 164 872 1382 1624 1978 164
Size: 708 510 242 354 2676
Fragments arranged by size:
2676 708 510 354 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 148 275 2073 2206 2665 2874 148
Size: 127 1798 133 459 209 1764
Fragments arranged by size:
1798 1764 459 209 133 127
TatI w'GTAC_w
Cuts at: 944 4005 944
Size: 3061 1429
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4007 4039 4007
Size: 32 4458
Tth111I GACn'n_nGTC
Cuts at: 262 2287 262
Size: 2025 2465
XbaI T'CTAG_A
Cuts at: 1521 1521
Size: 4490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 399 735 1281 1985 171
Size: 228 336 546 704 2676
Fragments arranged by size:
2676 704 546 336 228
XmaI C'CCGG_G
Cuts at: 1322 1510 1322
Size: 188 4302
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI