Restriction map for
pF1KB5008
(Circular) (Six-base) MAPSORT of: pF1KB5008.seq Check: 8786 from: 1 to: 3995
pF1KB5008 3995 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 120 667 120
Size: 547 3448
AccI GT'mk_AC
Cuts at: 1033 1033
Size: 3995
Acc65I G'GTAC_C
Cuts at: 1011 1011
Size: 3995
AclI AA'CG_TT
Cuts at: 3835 3835
Size: 3995
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1820 2252 2999 3267 1820
Size: 432 747 268 2548
Fragments arranged by size:
2548 747 432 268
AflIII A'CryG_T
Cuts at: 1347 2451 3428 1347
Size: 1104 977 1914
Fragments arranged by size:
1914 1104 977
AgeI A'CCGG_T
Cuts at: 2383 2383
Size: 3995
AleI CACnn'nnGTG
Cuts at: 880 880
Size: 3995
AlwNI CAG_nnn'CTG
Cuts at: 846 1300 2867 846
Size: 454 1567 1974
Fragments arranged by size:
1974 1567 454
ApaI G_GGCC'C
Cuts at: 216 932 216
Size: 716 3279
ApaLI G'TGCA_C
Cuts at: 2765 2765
Size: 3995
ApoI r'AATT_y
Cuts at: 999 3348 3604 999
Size: 2349 256 1390
Fragments arranged by size:
2349 1390 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3995
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
AvaI C'yCGr_G
Cuts at: 878 1015 878
Size: 137 3858
BamHI G'GATC_C
Cuts at: 1020 1020
Size: 3995
BanI G'GyrC_C
Cuts at: 648 934 1011 1425 1673 1708 648
Size: 286 77 414 248 35 2935
Fragments arranged by size:
2935 414 286 248 77 35
BanII G_rGCy'C
Cuts at: 216 428 923 932 1009 2039 216
Size: 212 495 9 77 1030 2172
Fragments arranged by size:
2172 1030 495 212 77 9
BbeI G_GCGC'C
Cuts at: 1429 1677 1429
Size: 248 3747
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 604 638 90
Size: 34 480 34 3447
Fragments arranged by size:
3447 480 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1893 2654 3995 1893
Size: 761 1341 1893
Fragments arranged by size:
1893 1341 761
BclI T'GATC_A
Cuts at: 776 1515 776
Size: 739 3256
BglI GCCn_nnn'nGGC
Cuts at: 286 286
Size: 3995
BglII A'GATC_T
Cuts at: 1510 3547 1510
Size: 2037 1958
BlpI GC'TnA_GC
Cuts at: 1107 1107
Size: 3995
Bme1580I G_kGCm'C
Cuts at: 216 627 653 932 1620 1713 2769 216
Size: 411 26 279 688 93 1056 1442
Fragments arranged by size:
1442 1056 688 411 279 93 26
BmrI ACTGGGnnnn_n'
Cuts at: 834 1207 1288 1623 3767 834
Size: 373 81 335 2144 1062
Fragments arranged by size:
2144 1062 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 3995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 275
Size: 3995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1171 2050 2542 2840 151
Size: 1020 879 492 298 1306
Fragments arranged by size:
1306 1020 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 339 3699 339
Size: 3360 635
BsaAI yAC'GTr
Cuts at: 753 1348 753
Size: 595 3400
BsaBI GATnn'nnATC
Cuts at: 6 1509 3546 6
Size: 1503 2037 455
Fragments arranged by size:
2037 1503 455
BsaHI Gr'CG_yC
Cuts at: 317 355 1426 1674 2376 3872 317
Size: 38 1071 248 702 1496 440
Fragments arranged by size:
1496 1071 702 440 248 38
BsaWI w'CCGG_w
Cuts at: 455 1184 1705 2383 2657 2804 3215 455
Size: 729 521 678 274 147 411 1235
Fragments arranged by size:
1235 729 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 943 1420 2178 2755 3251 943
Size: 477 758 577 496 1687
Fragments arranged by size:
1687 758 577 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 747 757 747
Size: 10 3985
BsiEI CG_ry'CG
Cuts at: 104 1537 1583 2791 104
Size: 1433 46 1208 1308
Fragments arranged by size:
1433 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 428 905 1009 1787 1977 2769 428
Size: 477 104 778 190 792 1654
Fragments arranged by size:
1654 792 778 477 190 104
BsmI GAATG_Cn'
Cuts at: 388 388
Size: 3995
Bsp1286I G_dGCh'C
Cuts at: 216 428 627 653 905 923 932 1009 1620
Size: 212 199 26 252 18 9 77 611
Cuts at: 1620 1713 1787 1977 2039 2769 216
Size: 93 74 190 62 730 1442
Fragments arranged by size:
1442 730 611 252 212 199 190 93
77 74 62 26 18 9
BspEI T'CCGG_A
Cuts at: 455 3215 455
Size: 2760 1235
BspHI T'CATG_A
Cuts at: 3171 3171
Size: 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 120 667 1045 1259 1561 1942 120
Size: 547 378 214 302 381 2173
Fragments arranged by size:
2173 547 381 378 302 214
BsrBI CCG'CTC
Cuts at: 50 252 2287 2341 3825 3990 50
Size: 202 2035 54 1484 165 55
Fragments arranged by size:
2035 1484 202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1907 1907
Size: 3995
BsrFI r'CCGG_y
Cuts at: 1993 2174 2383 1993
Size: 181 209 3605
Fragments arranged by size:
3605 209 181
BsrGI T'GTAC_A
Cuts at: 395 869 395
Size: 474 3521
BssHII G'CGCG_C
Cuts at: 143 145 143
Size: 2 3993
BssSI C'ACGA_G
Cuts at: 2266 2624 2266
Size: 358 3637
BstAPI GCAn_nnn'nTGC
Cuts at: 968 1372 968
Size: 404 3591
BstBI TT'CG_AA
Cuts at: 492 2356 492
Size: 1864 2131
BstYI r'GATC_y
Cuts at: 1 407 1020 1487 1510 1845 2091 3103 3189
Size: 406 613 467 23 335 246 1012 86
Cuts at: 3189 3201 3547 1
Size: 12 346 449
Fragments arranged by size:
1012 613 467 449 406 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1945 2126 2289 1945
Size: 181 163 3651
Fragments arranged by size:
3651 181 163
BtsI GCAGTG_nn'
Cuts at: 627 1238 627
Size: 611 3384
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 552 587 552
Size: 35 3960
DraI TTT'AAA
Cuts at: 994 994
Size: 3995
DraIII CAC_nnn'GTG
Cuts at: 698 698
Size: 3995
DrdI GACnn_nn'nnGTC
Cuts at: 1701 2559 1701
Size: 858 3137
EaeI y'GGCC_r
Cuts at: 1580 1754 2145 2172 3517 1580
Size: 174 391 27 1345 2058
Fragments arranged by size:
2058 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1580 1580
Size: 3995
EarI CTCTTCn'nnn_
Cuts at: 489 2018 2228 489
Size: 1529 210 2256
Fragments arranged by size:
2256 1529 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2203 2513 2659 3805 2203
Size: 310 146 1146 2393
Fragments arranged by size:
2393 1146 310 146
EcoICRI GAG'CTC
Cuts at: 426 1007 426
Size: 581 3414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 275 1820 2252 2999 3267 275
Size: 1545 432 747 268 1003
Fragments arranged by size:
1545 1003 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 212 213 928 1134 212
Size: 1 715 206 3073
Fragments arranged by size:
3073 715 206 1
EcoRI G'AATT_C
Cuts at: 999 999
Size: 3995
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2014 2014
Size: 3995
FspI TGC'GCA
Cuts at: 1776 1776
Size: 3995
HaeII r_GCGC'y
Cuts at: 1429 1677 2699 3678 1429
Size: 248 1022 979 1746
Fragments arranged by size:
1746 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 1837 1867 1869 1899 588
Size: 32 1217 30 2 30 2684
Fragments arranged by size:
2684 1217 32 30 30 2
HincII GTy'rAC
Cuts at: 690 1034 690
Size: 344 3651
KasI G'GCGC_C
Cuts at: 1425 1673 1425
Size: 248 3747
KpnI G_GTAC'C
Cuts at: 1015 1015
Size: 3995
MluI A'CGCG_T
Cuts at: 3428 3428
Size: 3995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2666 2850 2666
Size: 184 3811
MscI TGG'CCA
Cuts at: 1756 1756
Size: 3995
MslI CAynn'nnrTG
Cuts at: 328 346 751 805 880 895 1984 2111 328
Size: 18 405 54 75 15 1089 127 2212
Fragments arranged by size:
2212 1089 405 127 75 54 18 15
MspA1I CmG'CkG
Cuts at: 1106 1420 1780 2793 3038 1106
Size: 314 360 1013 245 2063
Fragments arranged by size:
2063 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2176 2176
Size: 3995
NarI GG'CG_CC
Cuts at: 1426 1674 1426
Size: 248 3747
NgoMIV G'CCGG_C
Cuts at: 2174 2174
Size: 3995
NspI r_CATG'y
Cuts at: 1048 1548 2455 1048
Size: 500 907 2588
Fragments arranged by size:
2588 907 500
PciI A'CATG_T
Cuts at: 2451 2451
Size: 3995
PflMI CCAn_nnn'nTGG
Cuts at: 846 846
Size: 3995
PmeI GTTT'AAAC
Cuts at: 994 994
Size: 3995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3963
PspOMI G'GGCC_C
Cuts at: 212 928 212
Size: 716 3279
PstI C_TGCA'G
Cuts at: 622 1042 1727 622
Size: 420 685 2890
Fragments arranged by size:
2890 685 420
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3995
PvuII CAG'CTG
Cuts at: 1420 1780 1420
Size: 360 3635
RsrII CG'GwC_CG
Cuts at: 2190 2190
Size: 3995
SacI G_AGCT'C
Cuts at: 428 1009 428
Size: 581 3414
SalI G'TCGA_C
Cuts at: 1032 1032
Size: 3995
SapI GCTCTTCn'nnn_
Cuts at: 2018 2228 2018
Size: 210 3785
SbfI CC_TGCA'GG
Cuts at: 1042 1042
Size: 3995
ScaI AGT'ACT
Cuts at: 3512 3512
Size: 3995
SfcI C'TryA_G
Cuts at: 33 618 1038 1723 2716 2907 33
Size: 585 420 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 420 191
SfoI GGC'GCC
Cuts at: 1427 1675 1427
Size: 248 3747
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3995
SmaI CCC'GGG
Cuts at: 880 1017 880
Size: 137 3858
SmlI C'TyrA_G
Cuts at: 166 1150 2065 2557 2819 166
Size: 984 915 492 262 1342
Fragments arranged by size:
1342 984 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3995
SphI G_CATG'C
Cuts at: 1048 1548 1048
Size: 500 3495
StyI C'CwwG_G
Cuts at: 402 1129 1483 402
Size: 727 354 2914
Fragments arranged by size:
2914 727 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1578 1711 2170 2379 1578
Size: 133 459 209 3194
Fragments arranged by size:
3194 459 209 133
TatI w'GTAC_w
Cuts at: 395 869 3510 395
Size: 474 2641 880
Fragments arranged by size:
2641 880 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3512 3544 3512
Size: 32 3963
Tth111I GACn'n_nGTC
Cuts at: 1792 1792
Size: 3995
XbaI T'CTAG_A
Cuts at: 1026 1026
Size: 3995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 945 1490 945
Size: 545 3450
XmaI C'CCGG_G
Cuts at: 878 1015 878
Size: 137 3858
XmnI GAAnn'nnTTC
Cuts at: 559 559
Size: 3995
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmBI BstEII BstXI BstZ17I Bsu36I BtgI
ClaI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI