Restriction map for
pF1KB5010
(Circular) (Six-base) MAPSORT of: pF1KB5010.seq Check: 1804 from: 1 to: 4970
pF1KB5010 4970 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1414 1521 1834 1414
Size: 107 313 4550
Fragments arranged by size:
4550 313 107
AccI GT'mk_AC
Cuts at: 2008 2008
Size: 4970
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 632 1970 2795 3227 3580 4242 278
Size: 354 1338 825 432 353 662 1006
Fragments arranged by size:
1338 1006 825 662 432 354 353
AfeI AGC'GCT
Cuts at: 157 1714 157
Size: 1557 3413
AflIII A'CryG_T
Cuts at: 2322 4122 4403 2322
Size: 1800 281 2889
Fragments arranged by size:
2889 1800 281
AleI CACnn'nnGTG
Cuts at: 584 1088 584
Size: 504 4466
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 541 509
Size: 32 4938
AlwNI CAG_nnn'CTG
Cuts at: 1389 2275 3713 1389
Size: 886 1438 2646
Fragments arranged by size:
2646 1438 886
ApaLI G'TGCA_C
Cuts at: 493 1093 1177 1261 3808 493
Size: 600 84 84 2547 1655
Fragments arranged by size:
2547 1655 600 84 84
ApoI r'AATT_y
Cuts at: 1974 4323 4579 1974
Size: 2349 256 2365
Fragments arranged by size:
2365 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 159 397 451 930 984 1266 1990 159
Size: 238 54 479 54 282 724 3139
Fragments arranged by size:
3139 724 479 282 238 54 54
AvrII C'CTAG_G
Cuts at: 349 349
Size: 4970
BamHI G'GATC_C
Cuts at: 1995 1995
Size: 4970
BanI G'GyrC_C
Cuts at: 393 1986 2400 2648 2683 393
Size: 1593 414 248 35 2680
Fragments arranged by size:
2680 1593 414 248 35
BanII G_rGCy'C
Cuts at: 731 1730 1925 1984 3014 731
Size: 999 195 59 1030 2687
Fragments arranged by size:
2687 1030 999 195 59
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3924 4970 2868
Size: 1056 1046 2868
Fragments arranged by size:
2868 1056 1046
BclI T'GATC_A
Cuts at: 2490 2490
Size: 4970
BglI GCCn_nnn'nGGC
Cuts at: 596 1444 596
Size: 848 4122
BglII A'GATC_T
Cuts at: 2485 4522 2485
Size: 2037 2933
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 451 497 955 1097 1181 1265 1376 1898 2595
Size: 46 458 142 84 84 111 522 697
Cuts at: 2595 2688 3812 451
Size: 93 1124 1609
Fragments arranged by size:
1609 1124 697 522 458 142 111 93
84 84 46
BmrI ACTGGGnnnn_n'
Cuts at: 1644 1743 2182 2263 2598 4742 1644
Size: 99 439 81 335 2144 1872
Fragments arranged by size:
2144 1872 439 335 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1698 3337 1698
Size: 1639 3331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1939 1457
Size: 482 4488
Bpu10I CC'TnA_GC
Cuts at: 299 299
Size: 4970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 715 1144 1872 2146 3025 3739 4037 556
Size: 159 429 728 274 879 714 298 1489
Fragments arranged by size:
1489 879 728 714 429 298 274 159
BsaI GGTCTCn'nnnn_
Cuts at: 1669 4674 1669
Size: 3005 1965
BsaAI yAC'GTr
Cuts at: 827 2323 827
Size: 1496 3474
BsaBI GATnn'nnATC
Cuts at: 6 2484 4521 6
Size: 2478 2037 455
Fragments arranged by size:
2478 2037 455
BsaHI Gr'CG_yC
Cuts at: 1147 2401 2649 3351 4847 1147
Size: 1254 248 702 1496 1270
Fragments arranged by size:
1496 1270 1254 702 248
BsaWI w'CCGG_w
Cuts at: 2159 2680 3358 3769 3916 4190 2159
Size: 521 678 411 147 274 2939
Fragments arranged by size:
2939 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 313 343 313
Size: 30 4940
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1222 1642 1222
Size: 420 4550
BseYI C'CCAG_C
Cuts at: 138 1461 1777 2395 3153 3818 4226 138
Size: 1323 316 618 758 665 408 882
Fragments arranged by size:
1323 882 758 665 618 408 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1364 1728 1364
Size: 364 4606
BsiEI CG_ry'CG
Cuts at: 104 462 966 1107 1111 1964 2512 2558 3788
Size: 358 504 141 4 853 548 46 1230
Cuts at: 3788 104
Size: 1286
Fragments arranged by size:
1286 1230 853 548 504 358 141 46
4
BsiHKAI G_wGCw'C
Cuts at: 497 731 1097 1181 1265 1730 1984 2762 2952
Size: 234 366 84 84 465 254 778 190
Cuts at: 2952 3812 497
Size: 860 1655
Fragments arranged by size:
1655 860 778 465 366 254 234 190
84 84
BsmI GAATG_Cn'
Cuts at: 1567 1949 1567
Size: 382 4588
Bsp1286I G_dGCh'C
Cuts at: 451 497 731 955 1097 1181 1265 1376 1730
Size: 46 234 224 142 84 84 111 354
Cuts at: 1730 1898 1925 1984 2595 2688 2762 2952 3014
Size: 168 27 59 611 93 74 190 62
Cuts at: 3014 3812 451
Size: 798 1609
Fragments arranged by size:
1609 798 611 354 234 224 190 168
142 111 93 84 84 74 62 59
46 27
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1414 1521 1834 2020 2234 2536 2917 1414
Size: 107 313 186 214 302 381 3467
Fragments arranged by size:
3467 381 313 302 214 186 107
BsrBI CCG'CTC
Cuts at: 50 603 753 812 1341 3262 3316 4800 4965
Size: 553 150 59 529 1921 54 1484 165
Cuts at: 4965 50
Size: 55
Fragments arranged by size:
1921 1484 553 529 165 150 59 55
54
BsrDI GCAATG_nn'
Cuts at: 1132 2882 1132
Size: 1750 3220
BsrFI r'CCGG_y
Cuts at: 243 665 763 1065 1085 1106 1959 2968 3149
Size: 422 98 302 20 21 853 1009 181
Cuts at: 3149 243
Size: 2064
Fragments arranged by size:
2064 1009 853 422 302 181 98 21
20
BssHII G'CGCG_C
Cuts at: 220 557 1005 220
Size: 337 448 4185
Fragments arranged by size:
4185 448 337
BssSI C'ACGA_G
Cuts at: 916 1336 1708 3241 3949 916
Size: 420 372 1533 708 1937
Fragments arranged by size:
1937 1533 708 420 372
BstAPI GCAn_nnn'nTGC
Cuts at: 1751 2347 1751
Size: 596 4374
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 114 586 1573 1917 114
Size: 472 987 344 3167
Fragments arranged by size:
3167 987 472 344
BstYI r'GATC_y
Cuts at: 1 575 1995 2462 2485 2820 3066 3372 3384
Size: 574 1420 467 23 335 246 306 12
Cuts at: 3384 3470 4522 1
Size: 86 1052 449
Fragments arranged by size:
1420 1052 574 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1327 1327
Size: 4970
BtgI C'CryG_G
Cuts at: 107 338 411 1364 1898 107
Size: 231 73 953 534 3179
Fragments arranged by size:
3179 953 534 231 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 537 1032 1362 2213 537
Size: 495 330 851 3294
Fragments arranged by size:
3294 851 495 330
DraI TTT'AAA
Cuts at: 1969 1969
Size: 4970
DraIII CAC_nnn'GTG
Cuts at: 1526 1751 1893 1526
Size: 225 142 4603
Fragments arranged by size:
4603 225 142
DrdI GACnn_nn'nnGTC
Cuts at: 2676 4020 2676
Size: 1344 3626
EaeI y'GGCC_r
Cuts at: 266 341 459 588 963 1076 1108 1185 1517
Size: 75 118 129 375 113 32 77 332
Cuts at: 1517 1961 2555 2729 3120 3147 4492 266
Size: 444 594 174 391 27 1345 744
Fragments arranged by size:
1345 744 594 444 391 375 332 174
129 118 113 77 75 32 27
EagI C'GGCC_G
Cuts at: 459 963 1108 1961 2555 459
Size: 504 145 853 594 2874
Fragments arranged by size:
2874 853 594 504 145
EarI CTCTTCn'nnn_
Cuts at: 254 793 1946 2993 3203 254
Size: 539 1153 1047 210 2021
Fragments arranged by size:
2021 1153 1047 539 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1084 3178 3920 4066 4780 1084
Size: 2094 742 146 714 1274
Fragments arranged by size:
2094 1274 742 714 146
EcoICRI GAG'CTC
Cuts at: 729 1728 1982 729
Size: 999 254 3717
Fragments arranged by size:
3717 999 254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 632 1457 1939 1970 2795 3227 3580 4242
Size: 354 825 482 31 825 432 353 662
Cuts at: 4242 278
Size: 1006
Fragments arranged by size:
1006 825 825 662 482 432 354 353
31
EcoNI CCTnn'n_nnAGG
Cuts at: 1475 1475
Size: 4970
EcoO109I rG'GnC_Cy
Cuts at: 353 735 1155 1467 1904 2109 353
Size: 382 420 312 437 205 3214
Fragments arranged by size:
3214 437 420 382 312 205
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1695 1866 2989 1695
Size: 171 1123 3676
Fragments arranged by size:
3676 1123 171
FseI GG_CCGG'CC
Cuts at: 1963 1963
Size: 4970
FspI TGC'GCA
Cuts at: 406 536 1786 1870 2751 406
Size: 130 1250 84 881 2625
Fragments arranged by size:
2625 1250 881 130 84
FspAI rTGC'GCAy
Cuts at: 536 536
Size: 4970
HaeII r_GCGC'y
Cuts at: 159 774 1278 1408 1716 2404 2652 3882 4653
Size: 615 504 130 308 688 248 1230 771
Cuts at: 4653 159
Size: 476
Fragments arranged by size:
1230 771 688 615 504 476 308 248
130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 118 150 313 345 978 1010 2812 2842 2844
Size: 32 163 32 633 32 1802 30 2
Cuts at: 2844 2874 118
Size: 30 2214
Fragments arranged by size:
2214 1802 633 163 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2009 2009
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1001 3729 3913 1001
Size: 2728 184 2058
Fragments arranged by size:
2728 2058 184
MscI TGG'CCA
Cuts at: 590 1078 1187 1519 2731 590
Size: 488 109 332 1212 2829
Fragments arranged by size:
2829 1212 488 332 109
MslI CAynn'nnrTG
Cuts at: 112 584 1088 1546 2959 3086 112
Size: 472 504 458 1413 127 1996
Fragments arranged by size:
1996 1413 504 472 458 127
MspA1I CmG'CkG
Cuts at: 340 530 698 866 1191 1202 1366 2081 2395
Size: 190 168 168 325 11 164 715 314
Cuts at: 2395 2755 3539 3784 340
Size: 360 784 245 1526
Fragments arranged by size:
1526 784 715 360 325 314 245 190
168 168 164 11
NaeI GCC'GGC
Cuts at: 245 1067 1961 3151 245
Size: 822 894 1190 2064
Fragments arranged by size:
2064 1190 894 822
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 107 1898 107
Size: 1791 3179
NgoMIV G'CCGG_C
Cuts at: 243 1065 1959 3149 243
Size: 822 894 1190 2064
Fragments arranged by size:
2064 1190 894 822
NotI GC'GGCC_GC
Cuts at: 459 963 459
Size: 504 4466
NspI r_CATG'y
Cuts at: 972 1298 1558 2023 2523 4126 972
Size: 326 260 465 500 1603 1816
Fragments arranged by size:
1816 1603 500 465 326 260
PasI CC'CwG_GG
Cuts at: 212 212
Size: 4970
PciI A'CATG_T
Cuts at: 4122 4122
Size: 4970
PflMI CCAn_nnn'nTGG
Cuts at: 1526 1526
Size: 4970
PfoI T'CCnGG_A
Cuts at: 1580 1580
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PmlI CAC'GTG
Cuts at: 827 827
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3448 3416
Size: 32 4938
PpuMI rG'GwC_Cy
Cuts at: 735 1155 1467 1904 735
Size: 420 312 437 3801
Fragments arranged by size:
3801 437 420 312
PspXI vC'TCGA_Gb
Cuts at: 984 984
Size: 4970
PstI C_TGCA'G
Cuts at: 126 2017 2702 126
Size: 1891 685 2394
Fragments arranged by size:
2394 1891 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 698 2395 2755 698
Size: 1697 360 2913
Fragments arranged by size:
2913 1697 360
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 731 1730 1984 731
Size: 999 254 3717
Fragments arranged by size:
3717 999 254
SacII CC_GC'GG
Cuts at: 341 1367 341
Size: 1026 3944
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SanDI GG'GwC_CC
Cuts at: 1467 1904 1467
Size: 437 4533
SapI GCTCTTCn'nnn_
Cuts at: 793 2993 3203 793
Size: 2200 210 2560
Fragments arranged by size:
2560 2200 210
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 4487 4487
Size: 4970
SfcI C'TryA_G
Cuts at: 33 122 693 2013 2698 3666 3857 33
Size: 89 571 1320 685 968 191 1146
Fragments arranged by size:
1320 1146 968 685 571 191 89
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SgrAI Cr'CCGG_yG
Cuts at: 665 763 1085 665
Size: 98 322 4550
Fragments arranged by size:
4550 322 98
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 571 730 984 1159 1851 2125 3040 3754 4016
Size: 159 254 175 692 274 915 714 262
Cuts at: 4016 571
Size: 1525
Fragments arranged by size:
1525 915 714 692 274 262 254 175
159
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 2023 2523 2023
Size: 500 4470
StuI AGG'CCT
Cuts at: 382 550 1846 382
Size: 168 1296 3506
Fragments arranged by size:
3506 1296 168
StyI C'CwwG_G
Cuts at: 107 349 377 1598 1841 1898 2104 2458 107
Size: 242 28 1221 243 57 206 354 2619
Fragments arranged by size:
2619 1221 354 243 242 206 57 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1762 1900 2553 2686 3145 3354 1762
Size: 138 653 133 459 209 3378
Fragments arranged by size:
3378 653 459 209 138 133
TatI w'GTAC_w
Cuts at: 4485 4485
Size: 4970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4487 4519 4487
Size: 32 4938
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2465 2465
Size: 4970
XhoI C'TCGA_G
Cuts at: 984 984
Size: 4970
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
XmnI GAAnn'nnTTC
Cuts at: 802 943 802
Size: 141 4829
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI ApaI AscI BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmBI
BspEI BsrGI BstEII BstZ17I ClaI CspCI EcoRV HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PshAI
PsiI PspOMI PsrI SexAI SfiI SnaBI SrfI SspI
SwaI ZraI